P39735 (SAW1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Single-strand annealing weakened protein 1

Gene names

Name:	SAW1
Ordered Locus Names:	YAL027W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes 3'-non-homologous tail removal of RAD1/RAD10-dependent single-strand annealing recombination intermediates. Plays a key role in targeting RAD1/RAD10 complex to 3'-flap cleavage substrate in recombination. Also contributes to the integrity of ribosomal DNA arrays. Ref.7
Subunit structure	Interacts with MSH2, MSH3, RAD1, RAD10, RAD51 and RAD52. Ref.6 Ref.7
Subcellular location	Nucleus Ref.4.
Disruption phenotype	Accumulates recombination intermediates blocked at the RAD1/RAD10-dependent 3' flap cleavage step. Abolishes association of RAD1 at single-strand annealing (SSA) intermediates. Insensitive to MMS, HU, or phleomycin treatment. Doesn't sensitize cells to UV lesions. Substantially increases the rDNA recombination rate. Ref.7
Miscellaneous	Present with 1170 molecules/cell in log phase SD medium. Ref.5

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	double-strand break repair via single-strand annealing, removal of nonhomologous ends Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	nucleus Inferred from direct assay Ref.4. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RAD1	P06777	5	EBI-20627,EBI-14752

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Single-strand annealing weakened protein 1		PRO_0000202414

Sequences

	Sequence		Length	Mass (Da)
	P39735 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: F525EF2E5347E500	FASTA	261	29,770
	10 20 30 40 50 60 MAPSIATVKI ARDMVLPLRI FVNRKQILQT NDKTSNKSNA TIFEAPLLSN NSIICLKSPN 70 80 90 100 110 120 TRIYLSQQDK KNLCDEIKED LLLIVYELAS PEIISSVLSK IRVGHSTDFQ INVLPKLFAG 130 140 150 160 170 180 ADTDNAVTSH IQSVTRLAKF KYKLHYKHKW ELDIFINSIK KIANLRHYLM FQTLTLNGFS 190 200 210 220 230 240 LNAGPKTLLA RKIEKQPQVP NLLIENGDAD ALDTPVEEDI KPVIEFMYKP VINLGEIIDV 250 260 HVLHRPRRHK VRTQSKQPQE E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Examining protein protein interactions using endogenously tagged yeast arrays: the cross-and-capture system." Suter B., Fetchko M.J., Imhof R., Graham C.I., Stoffel-Studer I., Zbinden C., Raghavan M., Lopez L., Beneti L., Hort J., Fillingham J., Greenblatt J.F., Giaever G., Nislow C., Stagljar I. Genome Res. 17:1774-1782(2007) [PubMed: 17989249] [Abstract] Cited for: INTERACTION WITH RAD1 AND RAD10.
[7]	"Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates." Li F., Dong J., Pan X., Oum J.-H., Boeke J.D., Lee S.E. Mol. Cell 30:325-335(2008) [PubMed: 18471978] [Abstract] Cited for: FUNCTION, INTERACTION WITH RAD1; MSH2; MSH3; RAD51 AND RAD52, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12980 Genomic DNA. Translation: AAC05005.1. AY692559 Genomic DNA. Translation: AAT92578.1. BK006935 Genomic DNA. Translation: DAA06961.1.
PIR	S51994.
RefSeq	NP_009375.1. NM_001178172.1.
3D structure databases
ProteinModelPortal	P39735.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6595N.
IntAct	P39735. 22 interactions.
MINT	MINT-622986.
STRING	P39735.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL027W; YAL027W; YAL027W.
GeneID	851206.
KEGG	sce:YAL027W.
NMPDR	fig|4932.3.peg.55.
Organism-specific databases
CYGD	YAL027w.
SGD	S000000025. SAW1.
Phylogenomic databases
eggNOG	fuNOG11874.
HOGENOM	HBG512041.
OMA	ATIFEAP.
OrthoDB	EOG4NKG54.
Gene expression databases
ArrayExpress	P39735.
Genevestigator	P39735.
GermOnline	YAL027W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR021624. Saw1. [Graphical view]
KO	K15080.
Pfam	PF11561. Saw1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968073.

Entry information

Entry name

SAW1_YEAST

Accession

Primary (citable) accession number: P39735
Secondary accession number(s): D6VPJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program