P39734 (HPH2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein HPH2

Alternative name(s):
Functionally related to TCP1 protein 2
High pH protein 2

Gene names

Name:	FRT2
Synonyms:	HPH2
Ordered Locus Names:	YAL028W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for growth under high NaCl, alkaline pH and cell wall stress. Ref.5
Subunit structure	Interacts with HPH1/FRT1. Ref.5
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein. Note: Punctate foci at the endoplasmic reticulum membrane. Ref.5
Post-translational modification	Phosphorylated by CDC28. Ref.4

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to stress Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	endoplasmic reticulum Inferred from direct assay Ref.5. Source: SGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 528	528	Protein HPH2		PRO_0000202415
Regions
	Transmembrane	505 – 521	17	Helical; Potential
	Compositional bias	308 – 328	21	Poly-Ser
	Compositional bias	512 – 517	6	Poly-Ile

Sequences

	Sequence		Length	Mass (Da)
	P39734 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: E5A3CC7C6D60977A	FASTA	528	58,750
	10 20 30 40 50 60 MQNAQIKSSS KGSGIDGTDR NSKDGVEKRP LEDVKQMIDA GTPDVGHKST VETKPNVGWQ 70 80 90 100 110 120 ASHSNLAALH EKEQKYEMEH HHARHKLHRQ VIPDYTSAST AMFSDCMFNA APDKVRSLST 130 140 150 160 170 180 MKSSGLSPKH PFNVVATFKG PFPQHSVESK PLDGGYSAKD HFPSFKMLQA QQHPAHRHYK 190 200 210 220 230 240 DNDKYGLKSP SRSFVKDKKR LVHRFLKSME PSSSGQSKDS SALAPAFDPI LPNVISKPSK 250 260 270 280 290 300 RPTHHSHSSD GSSSTQTDIS LQSLLYHDLE SSPKKHVSPS RPPSVASESS PAVANPIGLS 310 320 330 340 350 360 PKDACNASFS QSSSSSLSSS SSSSSSTSFS QSVAVDPLEP PGNITYSSSN LSLNSDELDY 370 380 390 400 410 420 YQRHIGLQLQ QTEALLKHSL KDEVLKDEND LVKNIANFDK IVKELRDLRS RTIGWKELVE 430 440 450 460 470 480 EDYLMNLKQD FDKENPESFE ARLSDTINTN VAKLQDLEKR MASCKDRLAS RKEVMRKMES 490 500 510 520 LLSLENSLMI SKKNVTFASK YRNEALDIVF LIIIIVICYT FKHLVSHK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Vo D.T. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Targets of the cyclin-dependent kinase Cdk1." Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O. Nature 425:859-864(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION BY CDC28.
[5]	"Hph1p and Hph2p, novel components of calcineurin-mediated stress responses in Saccharomyces cerevisiae." Heath V.L., Shaw S.L., Roy S., Cyert M.S. Eukaryot. Cell 3:695-704(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH HPH1, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12980 Genomic DNA. Translation: AAC05004.1. BK006935 Genomic DNA. Translation: DAA06960.1.
PIR	S70295.
RefSeq	NP_009374.1. NM_001178173.1.
3D structure databases
ProteinModelPortal	P39734.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1309N.
IntAct	P39734. 4 interactions.
MINT	MINT-397872.
Proteomic databases
PaxDb	P39734.
PeptideAtlas	P39734.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL028W; YAL028W; YAL028W.
GeneID	851205.
KEGG	sce:YAL028W.
Organism-specific databases
CYGD	YAL028w.
SGD	S000000026. FRT2.
Phylogenomic databases
eggNOG	NOG74487.
GeneTree	ENSGT00530000068925.
OrthoDB	EOG4H1F5H.
Enzyme and pathway databases
BioCyc	YEAST:G3O-28839-MONOMER.
Gene expression databases
Genevestigator	P39734.
GermOnline	YAL028W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR025752. HPH_family. [Graphical view]
Pfam	PF13694. Hph. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968070.

Entry information

Entry name

HPH2_YEAST

Accession

Primary (citable) accession number: P39734
Secondary accession number(s): D6VPJ0, P39733

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program