ID   GIP4_YEAST              Reviewed;         760 AA.
AC   P39732; D6VPI7;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   27-MAR-2024, entry version 156.
DE   RecName: Full=GLC7-interacting protein 4;
GN   Name=GIP4; Synonyms=FUN21; OrderedLocusNames=YAL031C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7731988; DOI=10.1073/pnas.92.9.3809;
RA   Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N.,
RA   Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J.,
RA   Storms R.K.;
RT   "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995).
RN   [2]
RP   SEQUENCE REVISION.
RA   Vo D.T.;
RL   Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 550-760.
RX   PubMed=1583694; DOI=10.1016/0022-2836(92)91025-k;
RA   Harris S.D., Cheng J., Pugh T.A., Pringle J.R.;
RT   "Molecular analysis of Saccharomyces cerevisiae chromosome I. On the number
RT   of genes and the identification of essential genes using temperature-
RT   sensitive-lethal mutations.";
RL   J. Mol. Biol. 225:53-65(1992).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [7]
RP   INTERACTION WITH GLC7, AND FUNCTION.
RX   PubMed=16537909; DOI=10.1128/mcb.26.7.2648-2660.2006;
RA   Pinsky B.A., Kotwaliwale C.V., Tatsutani S.Y., Breed C.A., Biggins S.;
RT   "Glc7/protein phosphatase 1 regulatory subunits can oppose the Ipl1/aurora
RT   protein kinase by redistributing Glc7.";
RL   Mol. Cell. Biol. 26:2648-2660(2006).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497 AND SER-501, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ADR376;
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-609, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [10]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-609, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: GLC7 phosphatase-regulatory protein involved in GLC7
CC       subcellular redistribution and chromosome segregation.
CC       {ECO:0000269|PubMed:16537909}.
CC   -!- SUBUNIT: Interacts with GLC7. {ECO:0000269|PubMed:16537909}.
CC   -!- INTERACTION:
CC       P39732; P32598: GLC7; NbExp=4; IntAct=EBI-20636, EBI-13715;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 227 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the GIP4 family. {ECO:0000305}.
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DR   EMBL; U12980; AAC05001.1; -; Genomic_DNA.
DR   EMBL; X62577; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BK006935; DAA06957.1; -; Genomic_DNA.
DR   PIR; S70294; S70294.
DR   RefSeq; NP_009371.1; NM_001178176.1.
DR   AlphaFoldDB; P39732; -.
DR   SMR; P39732; -.
DR   BioGRID; 31735; 62.
DR   DIP; DIP-6268N; -.
DR   IntAct; P39732; 3.
DR   MINT; P39732; -.
DR   STRING; 4932.YAL031C; -.
DR   iPTMnet; P39732; -.
DR   MaxQB; P39732; -.
DR   PaxDb; 4932-YAL031C; -.
DR   PeptideAtlas; P39732; -.
DR   EnsemblFungi; YAL031C_mRNA; YAL031C; YAL031C.
DR   GeneID; 851202; -.
DR   KEGG; sce:YAL031C; -.
DR   AGR; SGD:S000000029; -.
DR   SGD; S000000029; GIP4.
DR   VEuPathDB; FungiDB:YAL031C; -.
DR   eggNOG; ENOG502QRIU; Eukaryota.
DR   HOGENOM; CLU_021324_0_0_1; -.
DR   InParanoid; P39732; -.
DR   OMA; WYKKPAV; -.
DR   OrthoDB; 2034742at2759; -.
DR   BioCyc; YEAST:G3O-28842-MONOMER; -.
DR   BioGRID-ORCS; 851202; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P39732; -.
DR   Proteomes; UP000002311; Chromosome I.
DR   RNAct; P39732; Protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0008157; F:protein phosphatase 1 binding; IDA:SGD.
DR   GO; GO:0019888; F:protein phosphatase regulator activity; IMP:SGD.
DR   GO; GO:0007059; P:chromosome segregation; IMP:SGD.
DR   InterPro; IPR026241; GIP4.
DR   PRINTS; PR02082; GLC7IP4.
PE   1: Evidence at protein level;
KW   Cytoplasm; Phosphoprotein; Reference proteome.
FT   CHAIN           1..760
FT                   /note="GLC7-interacting protein 4"
FT                   /id="PRO_0000202416"
FT   REGION          449..573
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          593..626
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        455..515
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        531..573
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         497
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950"
FT   MOD_RES         501
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950"
FT   MOD_RES         609
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18407956,
FT                   ECO:0007744|PubMed:19779198"
SQ   SEQUENCE   760 AA;  86636 MW;  95C5C7DAD4B5D2C8 CRC64;
     MVDVQKRKKL LAKAAASASI PAIKGSVPLD SYDIKIIQYK NALYKLNELN RLLNVLVPHL
     KKKRDNDESY KIIPLVNFIL SLCEGPIFNV SPVLAKRYHL LCRFQLIKLS EVQQRLSTNF
     IDVEGWMFPE EVPLDHYKSC IYNNSLQWKI LNSLSCIAQN AIKIYNAKLR QILLERDAYK
     ARSLPFDTSI IEDLLNPVEM TLILDLAVLI NDPVRDKSTH SFYKLQWQVM EKLNSCVHSK
     IFPILRTYYN QLQKFSETRP TSLSNLQKDL PHWEWTLHRI YTFHLRVFSV LCVIISFSRQ
     IFLPNKQHFL DIKTRLSSEN VYHYDLIICE LMALLSPECD DVTALFELQE NLKFWTQTAR
     TDNNSSRTPI FHLQPGLVVE LFNNHICKII PKLRSIMGLL SNWMDCWKYI EKNYKTFDET
     NDLRENLKEK LERDKALYLE VKNAKSKLKK KPSITKLPAS SSPSPSPTSS ASPSRQASLE
     SIRTRARAHL ASNSSRSPSV SPVRTTFNNK NAETKKSVVS PEKRKLINGR RPRSSSLQSY
     TNKQQTSYLN STRHPSIAPP SKLNNQRSNS LQSSTMTLNQ KIVQDTVRHL MNKSASTPNP
     SASSSLAPSP KVSSINNTSS GKSSSTLIAN SSDTLAIETL TLDPESNSSE LSIKRVRFAG
     VPPMTEAENP KPTKVGWYKK PAVLHYPPIP ASAMIKPLQH KSKYNTLRQE EGFTFRKSLR
     DGLEWENGES GSETTMMPFG IEIKESTGHR IASKIRSKLR
//