P39732 (GIP4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GLC7-interacting protein 4

Gene names

Name:	GIP4
Synonyms:	FUN21
Ordered Locus Names:	YAL031C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	760 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	GLC7 phosphatase-regulatory protein involved in GLC7 subcellular redistribution and chromosome segregation. Ref.7
Subunit structure	Interacts with GLC7. Ref.7
Subcellular location	Cytoplasm Ref.5.
Miscellaneous	Present with 227 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the GIP4 family.

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chromosome segregation Inferred from mutant phenotype Ref.7. Source: SGD regulation of phosphoprotein phosphatase activity Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	cytoplasm Inferred from direct assay Ref.5. Source: SGD
Molecular function	protein phosphatase 1 binding Inferred from direct assay Ref.7. Source: SGD protein phosphatase type 1 regulator activity Inferred from mutant phenotype Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GLC7	P32598	4	EBI-20636,EBI-13715

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 760	760	GLC7-interacting protein 4		PRO_0000202416
Amino acid modifications
	Modified residue	462	1	Phosphoserine Ref.10
	Modified residue	464	1	Phosphoserine Ref.10
	Modified residue	492	1	Phosphoserine Ref.9
	Modified residue	497	1	Phosphoserine Ref.8 Ref.9
	Modified residue	501	1	Phosphoserine Ref.8 Ref.9
	Modified residue	536	1	Phosphoserine Ref.10
	Modified residue	570	1	Phosphoserine Ref.10
	Modified residue	609	1	Phosphoserine Ref.10
	Modified residue	649	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P39732 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 95C5C7DAD4B5D2C8	FASTA	760	86,636
	10 20 30 40 50 60 MVDVQKRKKL LAKAAASASI PAIKGSVPLD SYDIKIIQYK NALYKLNELN RLLNVLVPHL 70 80 90 100 110 120 KKKRDNDESY KIIPLVNFIL SLCEGPIFNV SPVLAKRYHL LCRFQLIKLS EVQQRLSTNF 130 140 150 160 170 180 IDVEGWMFPE EVPLDHYKSC IYNNSLQWKI LNSLSCIAQN AIKIYNAKLR QILLERDAYK 190 200 210 220 230 240 ARSLPFDTSI IEDLLNPVEM TLILDLAVLI NDPVRDKSTH SFYKLQWQVM EKLNSCVHSK 250 260 270 280 290 300 IFPILRTYYN QLQKFSETRP TSLSNLQKDL PHWEWTLHRI YTFHLRVFSV LCVIISFSRQ 310 320 330 340 350 360 IFLPNKQHFL DIKTRLSSEN VYHYDLIICE LMALLSPECD DVTALFELQE NLKFWTQTAR 370 380 390 400 410 420 TDNNSSRTPI FHLQPGLVVE LFNNHICKII PKLRSIMGLL SNWMDCWKYI EKNYKTFDET 430 440 450 460 470 480 NDLRENLKEK LERDKALYLE VKNAKSKLKK KPSITKLPAS SSPSPSPTSS ASPSRQASLE 490 500 510 520 530 540 SIRTRARAHL ASNSSRSPSV SPVRTTFNNK NAETKKSVVS PEKRKLINGR RPRSSSLQSY 550 560 570 580 590 600 TNKQQTSYLN STRHPSIAPP SKLNNQRSNS LQSSTMTLNQ KIVQDTVRHL MNKSASTPNP 610 620 630 640 650 660 SASSSLAPSP KVSSINNTSS GKSSSTLIAN SSDTLAIETL TLDPESNSSE LSIKRVRFAG 670 680 690 700 710 720 VPPMTEAENP KPTKVGWYKK PAVLHYPPIP ASAMIKPLQH KSKYNTLRQE EGFTFRKSLR 730 740 750 760 DGLEWENGES GSETTMMPFG IEIKESTGHR IASKIRSKLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Vo D.T. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Molecular analysis of Saccharomyces cerevisiae chromosome I. On the number of genes and the identification of essential genes using temperature-sensitive-lethal mutations." Harris S.D., Cheng J., Pugh T.A., Pringle J.R. J. Mol. Biol. 225:53-65(1992) [PubMed: 1583694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 550-760.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Glc7/protein phosphatase 1 regulatory subunits can oppose the Ipl1/aurora protein kinase by redistributing Glc7." Pinsky B.A., Kotwaliwale C.V., Tatsutani S.Y., Breed C.A., Biggins S. Mol. Cell. Biol. 26:2648-2660(2006) [PubMed: 16537909] [Abstract] Cited for: INTERACTION WITH GLC7, FUNCTION.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-497 AND SER-501, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-492; SER-497 AND SER-501, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-462; SER-464; SER-536; SER-570; SER-609 AND SER-649, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12980 Genomic DNA. Translation: AAC05001.1. X62577 Genomic DNA. No translation available. BK006935 Genomic DNA. Translation: DAA06957.1.
PIR	S70294.
RefSeq	NP_009371.1. NM_001178176.1.
3D structure databases
ProteinModelPortal	P39732.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6268N.
IntAct	P39732. 3 interactions.
MINT	MINT-597926.
STRING	P39732.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL031C; YAL031C; YAL031C.
GeneID	851202.
KEGG	sce:YAL031C.
NMPDR	fig|4932.3.peg.51.
Organism-specific databases
CYGD	YAL031c.
SGD	S000000029. GIP4.
Phylogenomic databases
eggNOG	fuNOG09815.
OMA	WYKKPAV.
OrthoDB	EOG4H1F4W.
Gene expression databases
ArrayExpress	P39732.
Genevestigator	P39732.
GermOnline	YAL031C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	968061.

Entry information

Entry name

GIP4_YEAST

Accession

Primary (citable) accession number: P39732
Secondary accession number(s): D6VPI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program