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P39730 (IF2P_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Eukaryotic translation initiation factor 5B

Short name=eIF-5B
Alternative name(s):
Translation initiation factor IF-2
Gene names
Name:FUN12
Ordered Locus Names:YAL035W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1002 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.

Miscellaneous

Present with 13400 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the IF-2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10021002Eukaryotic translation initiation factor 5B
PRO_0000137296

Regions

Nucleotide binding412 – 4198GTP By similarity
Compositional bias361 – 37111Poly-Glu

Amino acid modifications

Modified residue4051Phosphoserine Ref.8

Sequences

Sequence LengthMass (Da)Tools
P39730 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 1A496195DAE1C283

FASTA1,002112,268
        10         20         30         40         50         60 
MAKKSKKNQQ NYWDEEFEED AAQNEEISAT PTPNPESSAG ADDTSREASA SAEGAEAIEG 

        70         80         90        100        110        120 
DFMSTLKQSK KKQEKKVIEE KKDGKPILKS KKEKEKEKKE KEKQKKKEQA ARKKAQQQAQ 

       130        140        150        160        170        180 
KEKNKELNKQ NVEKAAAEKA AAEKSQKSKG ESDKPSASAK KPAKKVPAGL AALRRQLELK 

       190        200        210        220        230        240 
KQLEEQEKLE REEEERLEKE EEERLANEEK MKEEAKAAKK EKEKAKREKR KAEGKLLTRK 

       250        260        270        280        290        300 
QKEEKKLLER RRAALLSSGN VKVAGLAKKD GEENKPKKVV YSKKKKRTTQ ENASEAIKSD 

       310        320        330        340        350        360 
SKKDSEVVPD DELKESEDVL IDDWENLALG DDDEEGTNEE TQESTASHEN EDQNQGEEEE 

       370        380        390        400        410        420 
EGEEEEEEEE ERAHVHEVAK STPAATPAAT PTPSSASPNK KDLRSPICCI LGHVDTGKTK 

       430        440        450        460        470        480 
LLDKIRQTNV QGGEAGGITQ QIGATYFPID AIKAKTKVMA EYEKQTFDVP GLLVIDTPGH 

       490        500        510        520        530        540 
ESFSNLRSRG SSLCNIAILV IDIMHGLEQQ TIESIKLLRD RKAPFVVALN KIDRLYDWKA 

       550        560        570        580        590        600 
IPNNSFRDSF AKQSRAVQEE FQSRYSKIQL ELAEQGLNSE LYFQNKNMSK YVSIVPTSAV 

       610        620        630        640        650        660 
TGEGVPDLLW LLLELTQKRM SKQLMYLSHV EATILEVKVV EGFGTTIDVI LSNGYLREGD 

       670        680        690        700        710        720 
RIVLCGMNGP IVTNIRALLT PQPLRELRLK SEYVHHKEVK AALGVKIAAN DLEKAVSGSR 

       730        740        750        760        770        780 
LLVVGPEDDE DELMDDVMDD LTGLLDSVDT TGKGVVVQAS TLGSLEALLD FLKDMKIPVM 

       790        800        810        820        830        840 
SIGLGPVYKR DVMKASTMLE KAPEYAVMLC FDVKVDKEAE QYAEQEGIKI FNADVIYHLF 

       850        860        870        880        890        900 
DSFTAYQEKL LEERRKDFLD YAIFPCVLQT LQIINKRGPM IIGVDVLEGT LRVGTPICAV 

       910        920        930        940        950        960 
KTDPTTKERQ TLILGKVISL EINHQPVQEV KKGQTAAGVA VRLEDPSGQQ PIWGRHVDEN 

       970        980        990       1000 
DTLYSLVSRR SIDTLKDKAF RDQVARSDWL LLKKLKVVFG IE 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Vo D.
Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
Strain: ATCC 204511 / S288c / AB972.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Isolation, identification and characterization of the FUN12 gene of Saccharomyces cerevisiae."
Sutrave P., Shafer B.K., Strathern J.N., Hughes S.H.
Gene 146:209-213(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Promotion of met-tRNAiMet binding to ribosomes by yIF2, a bacterial IF2 homolog in yeast."
Choi S.K., Lee J.H., Zoll W.L., Merrick W.C., Dever T.E.
Science 280:1757-1760(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-405, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12980 Genomic DNA. Translation: AAC04996.1.
L29389 Genomic DNA. Translation: AAA57228.1. Sequence problems.
BK006935 Genomic DNA. Translation: DAA06952.1.
PIRS70292.
RefSeqNP_009365.1. NM_001178180.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4BYRelectron microscopy4.30P401-739[»]
4BYXelectron microscopy6.60V401-739[»]
ProteinModelPortalP39730.
SMRP39730. Positions 403-983.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31730. 85 interactions.
DIPDIP-3790N.
IntActP39730. 50 interactions.
MINTMINT-508011.
STRING4932.YAL035W.

Proteomic databases

PaxDbP39730.
PeptideAtlasP39730.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAL035W; YAL035W; YAL035W.
GeneID851196.
KEGGsce:YAL035W.

Organism-specific databases

CYGDYAL035w.
SGDS000000033. FUN12.

Phylogenomic databases

eggNOGCOG0532.
GeneTreeENSGT00730000111064.
HOGENOMHOG000105770.
KOK03243.
OMARGEYIHH.
OrthoDBEOG73BVNC.

Enzyme and pathway databases

BioCycYEAST:G3O-28845-MONOMER.

Gene expression databases

GenevestigatorP39730.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
InterProIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SUPFAMSSF50447. SSF50447. 1 hit.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
ProtoNetSearch...

Other

NextBio968046.
PROP39730.

Entry information

Entry nameIF2P_YEAST
AccessionPrimary (citable) accession number: P39730
Secondary accession number(s): D6VPI2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Translation initiation factors

List of translation initiation factor entries