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P39730

- IF2P_YEAST

UniProt

P39730 - IF2P_YEAST

Protein

Eukaryotic translation initiation factor 5B

Gene

FUN12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi412 – 4198GTPBy similarity

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: SGD
    3. protein binding Source: SGD
    4. ribosome binding Source: SGD
    5. translation initiation factor activity Source: SGD
    6. translation initiation factor binding Source: SGD

    GO - Biological processi

    1. formation of translation initiation complex Source: SGD
    2. GTP catabolic process Source: GOC
    3. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
    4. regulation of translational initiation Source: SGD
    5. ribosome assembly Source: SGD

    Keywords - Molecular functioni

    Initiation factor

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28845-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 5B
    Short name:
    eIF-5B
    Alternative name(s):
    Translation initiation factor IF-2
    Gene namesi
    Name:FUN12
    Ordered Locus Names:YAL035W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAL035w.
    SGDiS000000033. FUN12.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosolic small ribosomal subunit Source: SGD
    2. eukaryotic 48S preinitiation complex Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10021002Eukaryotic translation initiation factor 5BPRO_0000137296Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei405 – 4051Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39730.
    PaxDbiP39730.
    PeptideAtlasiP39730.

    Expressioni

    Gene expression databases

    GenevestigatoriP39730.

    Interactioni

    Protein-protein interaction databases

    BioGridi31730. 85 interactions.
    DIPiDIP-3790N.
    IntActiP39730. 50 interactions.
    MINTiMINT-508011.
    STRINGi4932.YAL035W.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4BYRelectron microscopy4.30P401-739[»]
    4BYXelectron microscopy6.60V401-739[»]
    ProteinModelPortaliP39730.
    SMRiP39730. Positions 401-981.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini403 – 621219tr-type GPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni412 – 4198G1PROSITE-ProRule annotation
    Regioni437 – 4415G2PROSITE-ProRule annotation
    Regioni476 – 4794G3PROSITE-ProRule annotation
    Regioni530 – 5334G4PROSITE-ProRule annotation
    Regioni598 – 6003G5PROSITE-ProRule annotation

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi361 – 37111Poly-GluAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.PROSITE-ProRule annotation
    Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0532.
    GeneTreeiENSGT00730000111064.
    HOGENOMiHOG000105770.
    KOiK03243.
    OMAiGSLRMHT.
    OrthoDBiEOG73BVNC.

    Family and domain databases

    Gene3Di3.40.50.10050. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR023115. TIF_IF2_dom3.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF11987. IF-2. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF52156. SSF52156. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39730-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKKSKKNQQ NYWDEEFEED AAQNEEISAT PTPNPESSAG ADDTSREASA     50
    SAEGAEAIEG DFMSTLKQSK KKQEKKVIEE KKDGKPILKS KKEKEKEKKE 100
    KEKQKKKEQA ARKKAQQQAQ KEKNKELNKQ NVEKAAAEKA AAEKSQKSKG 150
    ESDKPSASAK KPAKKVPAGL AALRRQLELK KQLEEQEKLE REEEERLEKE 200
    EEERLANEEK MKEEAKAAKK EKEKAKREKR KAEGKLLTRK QKEEKKLLER 250
    RRAALLSSGN VKVAGLAKKD GEENKPKKVV YSKKKKRTTQ ENASEAIKSD 300
    SKKDSEVVPD DELKESEDVL IDDWENLALG DDDEEGTNEE TQESTASHEN 350
    EDQNQGEEEE EGEEEEEEEE ERAHVHEVAK STPAATPAAT PTPSSASPNK 400
    KDLRSPICCI LGHVDTGKTK LLDKIRQTNV QGGEAGGITQ QIGATYFPID 450
    AIKAKTKVMA EYEKQTFDVP GLLVIDTPGH ESFSNLRSRG SSLCNIAILV 500
    IDIMHGLEQQ TIESIKLLRD RKAPFVVALN KIDRLYDWKA IPNNSFRDSF 550
    AKQSRAVQEE FQSRYSKIQL ELAEQGLNSE LYFQNKNMSK YVSIVPTSAV 600
    TGEGVPDLLW LLLELTQKRM SKQLMYLSHV EATILEVKVV EGFGTTIDVI 650
    LSNGYLREGD RIVLCGMNGP IVTNIRALLT PQPLRELRLK SEYVHHKEVK 700
    AALGVKIAAN DLEKAVSGSR LLVVGPEDDE DELMDDVMDD LTGLLDSVDT 750
    TGKGVVVQAS TLGSLEALLD FLKDMKIPVM SIGLGPVYKR DVMKASTMLE 800
    KAPEYAVMLC FDVKVDKEAE QYAEQEGIKI FNADVIYHLF DSFTAYQEKL 850
    LEERRKDFLD YAIFPCVLQT LQIINKRGPM IIGVDVLEGT LRVGTPICAV 900
    KTDPTTKERQ TLILGKVISL EINHQPVQEV KKGQTAAGVA VRLEDPSGQQ 950
    PIWGRHVDEN DTLYSLVSRR SIDTLKDKAF RDQVARSDWL LLKKLKVVFG 1000
    IE 1002
    Length:1,002
    Mass (Da):112,268
    Last modified:October 1, 1996 - v2
    Checksum:i1A496195DAE1C283
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12980 Genomic DNA. Translation: AAC04996.1.
    L29389 Genomic DNA. Translation: AAA57228.1. Sequence problems.
    BK006935 Genomic DNA. Translation: DAA06952.1.
    PIRiS70292.
    RefSeqiNP_009365.1. NM_001178180.1.

    Genome annotation databases

    EnsemblFungiiYAL035W; YAL035W; YAL035W.
    GeneIDi851196.
    KEGGisce:YAL035W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12980 Genomic DNA. Translation: AAC04996.1 .
    L29389 Genomic DNA. Translation: AAA57228.1 . Sequence problems.
    BK006935 Genomic DNA. Translation: DAA06952.1 .
    PIRi S70292.
    RefSeqi NP_009365.1. NM_001178180.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4BYR electron microscopy 4.30 P 401-739 [» ]
    4BYX electron microscopy 6.60 V 401-739 [» ]
    ProteinModelPortali P39730.
    SMRi P39730. Positions 401-981.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31730. 85 interactions.
    DIPi DIP-3790N.
    IntActi P39730. 50 interactions.
    MINTi MINT-508011.
    STRINGi 4932.YAL035W.

    Proteomic databases

    MaxQBi P39730.
    PaxDbi P39730.
    PeptideAtlasi P39730.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL035W ; YAL035W ; YAL035W .
    GeneIDi 851196.
    KEGGi sce:YAL035W.

    Organism-specific databases

    CYGDi YAL035w.
    SGDi S000000033. FUN12.

    Phylogenomic databases

    eggNOGi COG0532.
    GeneTreei ENSGT00730000111064.
    HOGENOMi HOG000105770.
    KOi K03243.
    OMAi GSLRMHT.
    OrthoDBi EOG73BVNC.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28845-MONOMER.

    Miscellaneous databases

    NextBioi 968046.
    PROi P39730.

    Gene expression databases

    Genevestigatori P39730.

    Family and domain databases

    Gene3Di 3.40.50.10050. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR023115. TIF_IF2_dom3.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF11987. IF-2. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF52156. SSF52156. 1 hit.
    SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Vo D.
      Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
      Strain: ATCC 204511 / S288c / AB972.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Isolation, identification and characterization of the FUN12 gene of Saccharomyces cerevisiae."
      Sutrave P., Shafer B.K., Strathern J.N., Hughes S.H.
      Gene 146:209-213(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "Promotion of met-tRNAiMet binding to ribosomes by yIF2, a bacterial IF2 homolog in yeast."
      Choi S.K., Lee J.H., Zoll W.L., Merrick W.C., Dever T.E.
      Science 280:1757-1760(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-405, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiIF2P_YEAST
    AccessioniPrimary (citable) accession number: P39730
    Secondary accession number(s): D6VPI2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 13400 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3