P39730 (IF2P_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eukaryotic translation initiation factor 5B
Short name=eIF-5B

Alternative name(s):
Translation initiation factor IF-2

Gene names

Name:	FUN12
Ordered Locus Names:	YAL035W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1002 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.
Miscellaneous	Present with 13400 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the IF-2 family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Initiation factor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype PubMed 22751017. Source: SGD regulation of translational initiation Inferred from mutant phenotype PubMed 12507428. Source: SGD
Cellular_component	cytosolic small ribosomal subunit Inferred from direct assay Ref.5. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD
Molecular_function	GTP binding Inferred from direct assay PubMed 21335519. Source: SGD GTPase activity Inferred from mutant phenotype PubMed 12471154PubMed 19029250. Source: SGD ribosome binding Inferred from direct assay PubMed 21335519. Source: SGD translation initiation factor activity Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1002	1002	Eukaryotic translation initiation factor 5B		PRO_0000137296
Regions
	Nucleotide binding	412 – 419	8	GTP By similarity
	Compositional bias	361 – 371	11	Poly-Glu
Amino acid modifications
	Modified residue	381	1	Phosphoserine Ref.7
	Modified residue	386	1	Phosphothreonine Ref.7
	Modified residue	390	1	Phosphothreonine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P39730 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 1A496195DAE1C283	FASTA	1,002	112,268
	10 20 30 40 50 60 MAKKSKKNQQ NYWDEEFEED AAQNEEISAT PTPNPESSAG ADDTSREASA SAEGAEAIEG 70 80 90 100 110 120 DFMSTLKQSK KKQEKKVIEE KKDGKPILKS KKEKEKEKKE KEKQKKKEQA ARKKAQQQAQ 130 140 150 160 170 180 KEKNKELNKQ NVEKAAAEKA AAEKSQKSKG ESDKPSASAK KPAKKVPAGL AALRRQLELK 190 200 210 220 230 240 KQLEEQEKLE REEEERLEKE EEERLANEEK MKEEAKAAKK EKEKAKREKR KAEGKLLTRK 250 260 270 280 290 300 QKEEKKLLER RRAALLSSGN VKVAGLAKKD GEENKPKKVV YSKKKKRTTQ ENASEAIKSD 310 320 330 340 350 360 SKKDSEVVPD DELKESEDVL IDDWENLALG DDDEEGTNEE TQESTASHEN EDQNQGEEEE 370 380 390 400 410 420 EGEEEEEEEE ERAHVHEVAK STPAATPAAT PTPSSASPNK KDLRSPICCI LGHVDTGKTK 430 440 450 460 470 480 LLDKIRQTNV QGGEAGGITQ QIGATYFPID AIKAKTKVMA EYEKQTFDVP GLLVIDTPGH 490 500 510 520 530 540 ESFSNLRSRG SSLCNIAILV IDIMHGLEQQ TIESIKLLRD RKAPFVVALN KIDRLYDWKA 550 560 570 580 590 600 IPNNSFRDSF AKQSRAVQEE FQSRYSKIQL ELAEQGLNSE LYFQNKNMSK YVSIVPTSAV 610 620 630 640 650 660 TGEGVPDLLW LLLELTQKRM SKQLMYLSHV EATILEVKVV EGFGTTIDVI LSNGYLREGD 670 680 690 700 710 720 RIVLCGMNGP IVTNIRALLT PQPLRELRLK SEYVHHKEVK AALGVKIAAN DLEKAVSGSR 730 740 750 760 770 780 LLVVGPEDDE DELMDDVMDD LTGLLDSVDT TGKGVVVQAS TLGSLEALLD FLKDMKIPVM 790 800 810 820 830 840 SIGLGPVYKR DVMKASTMLE KAPEYAVMLC FDVKVDKEAE QYAEQEGIKI FNADVIYHLF 850 860 870 880 890 900 DSFTAYQEKL LEERRKDFLD YAIFPCVLQT LQIINKRGPM IIGVDVLEGT LRVGTPICAV 910 920 930 940 950 960 KTDPTTKERQ TLILGKVISL EINHQPVQEV KKGQTAAGVA VRLEDPSGQQ PIWGRHVDEN 970 980 990 1000 DTLYSLVSRR SIDTLKDKAF RDQVARSDWL LLKKLKVVFG IE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Vo D. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Isolation, identification and characterization of the FUN12 gene of Saccharomyces cerevisiae." Sutrave P., Shafer B.K., Strathern J.N., Hughes S.H. Gene 146:209-213(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"Promotion of met-tRNAiMet binding to ribosomes by yIF2, a bacterial IF2 homolog in yeast." Choi S.K., Lee J.H., Zoll W.L., Merrick W.C., Dever T.E. Science 280:1757-1760(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-381; THR-386 AND THR-390, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12980 Genomic DNA. Translation: AAC04996.1. L29389 Genomic DNA. Translation: AAA57228.1. Sequence problems. BK006935 Genomic DNA. Translation: DAA06952.1.
PIR	S70292.
RefSeq	NP_009365.1. NM_001178180.1.
3D structure databases
ProteinModelPortal	P39730.
SMR	P39730. Positions 403-983.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3790N.
IntAct	P39730. 51 interactions.
MINT	MINT-508011.
STRING	4932.YAL035W.
Proteomic databases
PaxDb	P39730.
PeptideAtlas	P39730.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL035W; YAL035W; YAL035W.
GeneID	851196.
KEGG	sce:YAL035W.
Organism-specific databases
CYGD	YAL035w.
SGD	S000000033. FUN12.
Phylogenomic databases
eggNOG	COG0532.
GeneTree	ENSGT00690000102102.
HOGENOM	HOG000105770.
KO	K03243.
OMA	TRNANIR.
OrthoDB	EOG40311D.
Gene expression databases
Genevestigator	P39730.
GermOnline	YAL035W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.10050. 1 hit.
InterPro	IPR000795. EF_GTP-bd_dom. IPR005225. Small_GTP-bd_dom. IPR015760. TIF_IF2. IPR023115. TIF_IF2_dom3. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF41. PTHR23115:SF41. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF11987. IF-2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SUPFAM	SSF52156. TIF_IF2_dom3. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS01176. IF2. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	968046.

Entry information

Entry name

IF2P_YEAST

Accession

Primary (citable) accession number: P39730
Secondary accession number(s): D6VPI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program