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P39730

- IF2P_YEAST

UniProt

P39730 - IF2P_YEAST

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Protein

Eukaryotic translation initiation factor 5B

Gene

FUN12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi412 – 4198GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: SGD
  2. GTP binding Source: SGD
  3. ribosome binding Source: SGD
  4. translation initiation factor activity Source: SGD
  5. translation initiation factor binding Source: SGD

GO - Biological processi

  1. formation of cytoplasmic translation initiation complex Source: SGD
  2. GTP catabolic process Source: GOC
  3. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  4. regulation of translational initiation Source: SGD
  5. ribosome assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28845-MONOMER.
ReactomeiREACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5B
Short name:
eIF-5B
Alternative name(s):
Translation initiation factor IF-2
Gene namesi
Name:FUN12
Ordered Locus Names:YAL035W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL035w.
SGDiS000000033. FUN12.

Subcellular locationi

GO - Cellular componenti

  1. cytosolic small ribosomal subunit Source: SGD
  2. eukaryotic 48S preinitiation complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10021002Eukaryotic translation initiation factor 5BPRO_0000137296Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei405 – 4051Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39730.
PaxDbiP39730.
PeptideAtlasiP39730.

Expressioni

Gene expression databases

GenevestigatoriP39730.

Interactioni

Protein-protein interaction databases

BioGridi31730. 85 interactions.
DIPiDIP-3790N.
IntActiP39730. 50 interactions.
MINTiMINT-508011.
STRINGi4932.YAL035W.

Structurei

Secondary structure

1
1002
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi407 – 4115Combined sources
Helixi421 – 4255Combined sources
Beta strandi442 – 4487Combined sources
Helixi449 – 4568Combined sources
Helixi457 – 4615Combined sources
Beta strandi469 – 4757Combined sources
Beta strandi478 – 4803Combined sources
Helixi481 – 4833Combined sources
Helixi487 – 4893Combined sources
Helixi490 – 4934Combined sources
Beta strandi495 – 5028Combined sources
Turni503 – 5053Combined sources
Helixi509 – 52113Combined sources
Beta strandi525 – 5306Combined sources
Helixi532 – 5343Combined sources
Helixi546 – 5516Combined sources
Helixi555 – 57319Combined sources
Turni574 – 5763Combined sources
Beta strandi578 – 5814Combined sources
Helixi582 – 5843Combined sources
Turni588 – 5903Combined sources
Beta strandi591 – 5966Combined sources
Turni599 – 6013Combined sources
Helixi605 – 61915Combined sources
Turni621 – 6244Combined sources
Beta strandi631 – 64010Combined sources
Turni641 – 6433Combined sources
Beta strandi644 – 65714Combined sources
Beta strandi661 – 6666Combined sources
Beta strandi669 – 68113Combined sources
Helixi685 – 6884Combined sources
Beta strandi692 – 70918Combined sources
Beta strandi720 – 7234Combined sources
Beta strandi726 – 7283Combined sources
Helixi730 – 74617Combined sources
Beta strandi750 – 7534Combined sources
Beta strandi755 – 7617Combined sources
Helixi762 – 77413Combined sources
Beta strandi779 – 7813Combined sources
Beta strandi784 – 7874Combined sources
Helixi789 – 7968Combined sources
Helixi798 – 8014Combined sources
Beta strandi807 – 8126Combined sources
Helixi817 – 82610Combined sources
Beta strandi829 – 8357Combined sources
Helixi836 – 85116Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BYRelectron microscopy4.30P401-739[»]
4BYXelectron microscopy6.60V401-739[»]
4N3SX-ray1.83A/B399-852[»]
4NCFX-ray3.02A/B399-852[»]
ProteinModelPortaliP39730.
SMRiP39730. Positions 399-1002.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini403 – 621219tr-type GPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni412 – 4198G1PROSITE-ProRule annotation
Regioni437 – 4415G2PROSITE-ProRule annotation
Regioni476 – 4794G3PROSITE-ProRule annotation
Regioni530 – 5334G4PROSITE-ProRule annotation
Regioni598 – 6003G5PROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi361 – 37111Poly-GluAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0532.
GeneTreeiENSGT00730000111064.
HOGENOMiHOG000105770.
InParanoidiP39730.
KOiK03243.
OMAiGSLRMHT.
OrthoDBiEOG73BVNC.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39730-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAKKSKKNQQ NYWDEEFEED AAQNEEISAT PTPNPESSAG ADDTSREASA
60 70 80 90 100
SAEGAEAIEG DFMSTLKQSK KKQEKKVIEE KKDGKPILKS KKEKEKEKKE
110 120 130 140 150
KEKQKKKEQA ARKKAQQQAQ KEKNKELNKQ NVEKAAAEKA AAEKSQKSKG
160 170 180 190 200
ESDKPSASAK KPAKKVPAGL AALRRQLELK KQLEEQEKLE REEEERLEKE
210 220 230 240 250
EEERLANEEK MKEEAKAAKK EKEKAKREKR KAEGKLLTRK QKEEKKLLER
260 270 280 290 300
RRAALLSSGN VKVAGLAKKD GEENKPKKVV YSKKKKRTTQ ENASEAIKSD
310 320 330 340 350
SKKDSEVVPD DELKESEDVL IDDWENLALG DDDEEGTNEE TQESTASHEN
360 370 380 390 400
EDQNQGEEEE EGEEEEEEEE ERAHVHEVAK STPAATPAAT PTPSSASPNK
410 420 430 440 450
KDLRSPICCI LGHVDTGKTK LLDKIRQTNV QGGEAGGITQ QIGATYFPID
460 470 480 490 500
AIKAKTKVMA EYEKQTFDVP GLLVIDTPGH ESFSNLRSRG SSLCNIAILV
510 520 530 540 550
IDIMHGLEQQ TIESIKLLRD RKAPFVVALN KIDRLYDWKA IPNNSFRDSF
560 570 580 590 600
AKQSRAVQEE FQSRYSKIQL ELAEQGLNSE LYFQNKNMSK YVSIVPTSAV
610 620 630 640 650
TGEGVPDLLW LLLELTQKRM SKQLMYLSHV EATILEVKVV EGFGTTIDVI
660 670 680 690 700
LSNGYLREGD RIVLCGMNGP IVTNIRALLT PQPLRELRLK SEYVHHKEVK
710 720 730 740 750
AALGVKIAAN DLEKAVSGSR LLVVGPEDDE DELMDDVMDD LTGLLDSVDT
760 770 780 790 800
TGKGVVVQAS TLGSLEALLD FLKDMKIPVM SIGLGPVYKR DVMKASTMLE
810 820 830 840 850
KAPEYAVMLC FDVKVDKEAE QYAEQEGIKI FNADVIYHLF DSFTAYQEKL
860 870 880 890 900
LEERRKDFLD YAIFPCVLQT LQIINKRGPM IIGVDVLEGT LRVGTPICAV
910 920 930 940 950
KTDPTTKERQ TLILGKVISL EINHQPVQEV KKGQTAAGVA VRLEDPSGQQ
960 970 980 990 1000
PIWGRHVDEN DTLYSLVSRR SIDTLKDKAF RDQVARSDWL LLKKLKVVFG

IE
Length:1,002
Mass (Da):112,268
Last modified:October 1, 1996 - v2
Checksum:i1A496195DAE1C283
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. Translation: AAC04996.1.
L29389 Genomic DNA. Translation: AAA57228.1. Sequence problems.
BK006935 Genomic DNA. Translation: DAA06952.1.
PIRiS70292.
RefSeqiNP_009365.1. NM_001178180.1.

Genome annotation databases

EnsemblFungiiYAL035W; YAL035W; YAL035W.
GeneIDi851196.
KEGGisce:YAL035W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. Translation: AAC04996.1 .
L29389 Genomic DNA. Translation: AAA57228.1 . Sequence problems.
BK006935 Genomic DNA. Translation: DAA06952.1 .
PIRi S70292.
RefSeqi NP_009365.1. NM_001178180.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4BYR electron microscopy 4.30 P 401-739 [» ]
4BYX electron microscopy 6.60 V 401-739 [» ]
4N3S X-ray 1.83 A/B 399-852 [» ]
4NCF X-ray 3.02 A/B 399-852 [» ]
ProteinModelPortali P39730.
SMRi P39730. Positions 399-1002.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31730. 85 interactions.
DIPi DIP-3790N.
IntActi P39730. 50 interactions.
MINTi MINT-508011.
STRINGi 4932.YAL035W.

Proteomic databases

MaxQBi P39730.
PaxDbi P39730.
PeptideAtlasi P39730.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL035W ; YAL035W ; YAL035W .
GeneIDi 851196.
KEGGi sce:YAL035W.

Organism-specific databases

CYGDi YAL035w.
SGDi S000000033. FUN12.

Phylogenomic databases

eggNOGi COG0532.
GeneTreei ENSGT00730000111064.
HOGENOMi HOG000105770.
InParanoidi P39730.
KOi K03243.
OMAi GSLRMHT.
OrthoDBi EOG73BVNC.

Enzyme and pathway databases

BioCyci YEAST:G3O-28845-MONOMER.
Reactomei REACT_232946. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

NextBioi 968046.
PROi P39730.

Gene expression databases

Genevestigatori P39730.

Family and domain databases

Gene3Di 3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
Pfami PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Vo D.
    Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
    Strain: ATCC 204511 / S288c / AB972.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Isolation, identification and characterization of the FUN12 gene of Saccharomyces cerevisiae."
    Sutrave P., Shafer B.K., Strathern J.N., Hughes S.H.
    Gene 146:209-213(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Promotion of met-tRNAiMet binding to ribosomes by yIF2, a bacterial IF2 homolog in yeast."
    Choi S.K., Lee J.H., Zoll W.L., Merrick W.C., Dever T.E.
    Science 280:1757-1760(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-405, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIF2P_YEAST
AccessioniPrimary (citable) accession number: P39730
Secondary accession number(s): D6VPI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13400 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3