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P39730

- IF2P_YEAST

UniProt

P39730 - IF2P_YEAST

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Protein
Eukaryotic translation initiation factor 5B
Gene
FUN12, YAL035W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi412 – 4198GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: SGD
  2. GTPase activity Source: SGD
  3. protein binding Source: SGD
  4. ribosome binding Source: SGD
  5. translation initiation factor activity Source: SGD
  6. translation initiation factor binding Source: SGD
Complete GO annotation...

GO - Biological processi

  1. GTP catabolic process Source: GOC
  2. formation of translation initiation complex Source: SGD
  3. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
  4. regulation of translational initiation Source: SGD
  5. ribosome assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28845-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5B
Short name:
eIF-5B
Alternative name(s):
Translation initiation factor IF-2
Gene namesi
Name:FUN12
Ordered Locus Names:YAL035W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL035w.
SGDiS000000033. FUN12.

Subcellular locationi

GO - Cellular componenti

  1. cytosolic small ribosomal subunit Source: SGD
  2. eukaryotic 48S preinitiation complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10021002Eukaryotic translation initiation factor 5B
PRO_0000137296Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei405 – 4051Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39730.
PaxDbiP39730.
PeptideAtlasiP39730.

Expressioni

Gene expression databases

GenevestigatoriP39730.

Interactioni

Protein-protein interaction databases

BioGridi31730. 85 interactions.
DIPiDIP-3790N.
IntActiP39730. 50 interactions.
MINTiMINT-508011.
STRINGi4932.YAL035W.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BYRelectron microscopy4.30P401-739[»]
4BYXelectron microscopy6.60V401-739[»]
ProteinModelPortaliP39730.
SMRiP39730. Positions 401-981.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini403 – 621219tr-type G
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni412 – 4198G1 By similarity
Regioni437 – 4415G2 By similarity
Regioni476 – 4794G3 By similarity
Regioni530 – 5334G4 By similarity
Regioni598 – 6003G5 By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi361 – 37111Poly-Glu
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0532.
GeneTreeiENSGT00730000111064.
HOGENOMiHOG000105770.
KOiK03243.
OMAiGSLRMHT.
OrthoDBiEOG73BVNC.

Family and domain databases

Gene3Di3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39730-1 [UniParc]FASTAAdd to Basket

« Hide

MAKKSKKNQQ NYWDEEFEED AAQNEEISAT PTPNPESSAG ADDTSREASA     50
SAEGAEAIEG DFMSTLKQSK KKQEKKVIEE KKDGKPILKS KKEKEKEKKE 100
KEKQKKKEQA ARKKAQQQAQ KEKNKELNKQ NVEKAAAEKA AAEKSQKSKG 150
ESDKPSASAK KPAKKVPAGL AALRRQLELK KQLEEQEKLE REEEERLEKE 200
EEERLANEEK MKEEAKAAKK EKEKAKREKR KAEGKLLTRK QKEEKKLLER 250
RRAALLSSGN VKVAGLAKKD GEENKPKKVV YSKKKKRTTQ ENASEAIKSD 300
SKKDSEVVPD DELKESEDVL IDDWENLALG DDDEEGTNEE TQESTASHEN 350
EDQNQGEEEE EGEEEEEEEE ERAHVHEVAK STPAATPAAT PTPSSASPNK 400
KDLRSPICCI LGHVDTGKTK LLDKIRQTNV QGGEAGGITQ QIGATYFPID 450
AIKAKTKVMA EYEKQTFDVP GLLVIDTPGH ESFSNLRSRG SSLCNIAILV 500
IDIMHGLEQQ TIESIKLLRD RKAPFVVALN KIDRLYDWKA IPNNSFRDSF 550
AKQSRAVQEE FQSRYSKIQL ELAEQGLNSE LYFQNKNMSK YVSIVPTSAV 600
TGEGVPDLLW LLLELTQKRM SKQLMYLSHV EATILEVKVV EGFGTTIDVI 650
LSNGYLREGD RIVLCGMNGP IVTNIRALLT PQPLRELRLK SEYVHHKEVK 700
AALGVKIAAN DLEKAVSGSR LLVVGPEDDE DELMDDVMDD LTGLLDSVDT 750
TGKGVVVQAS TLGSLEALLD FLKDMKIPVM SIGLGPVYKR DVMKASTMLE 800
KAPEYAVMLC FDVKVDKEAE QYAEQEGIKI FNADVIYHLF DSFTAYQEKL 850
LEERRKDFLD YAIFPCVLQT LQIINKRGPM IIGVDVLEGT LRVGTPICAV 900
KTDPTTKERQ TLILGKVISL EINHQPVQEV KKGQTAAGVA VRLEDPSGQQ 950
PIWGRHVDEN DTLYSLVSRR SIDTLKDKAF RDQVARSDWL LLKKLKVVFG 1000
IE 1002
Length:1,002
Mass (Da):112,268
Last modified:October 1, 1996 - v2
Checksum:i1A496195DAE1C283
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12980 Genomic DNA. Translation: AAC04996.1.
L29389 Genomic DNA. Translation: AAA57228.1. Sequence problems.
BK006935 Genomic DNA. Translation: DAA06952.1.
PIRiS70292.
RefSeqiNP_009365.1. NM_001178180.1.

Genome annotation databases

EnsemblFungiiYAL035W; YAL035W; YAL035W.
GeneIDi851196.
KEGGisce:YAL035W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12980 Genomic DNA. Translation: AAC04996.1 .
L29389 Genomic DNA. Translation: AAA57228.1 . Sequence problems.
BK006935 Genomic DNA. Translation: DAA06952.1 .
PIRi S70292.
RefSeqi NP_009365.1. NM_001178180.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4BYR electron microscopy 4.30 P 401-739 [» ]
4BYX electron microscopy 6.60 V 401-739 [» ]
ProteinModelPortali P39730.
SMRi P39730. Positions 401-981.
ModBasei Search...

Protein-protein interaction databases

BioGridi 31730. 85 interactions.
DIPi DIP-3790N.
IntActi P39730. 50 interactions.
MINTi MINT-508011.
STRINGi 4932.YAL035W.

Proteomic databases

MaxQBi P39730.
PaxDbi P39730.
PeptideAtlasi P39730.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL035W ; YAL035W ; YAL035W .
GeneIDi 851196.
KEGGi sce:YAL035W.

Organism-specific databases

CYGDi YAL035w.
SGDi S000000033. FUN12.

Phylogenomic databases

eggNOGi COG0532.
GeneTreei ENSGT00730000111064.
HOGENOMi HOG000105770.
KOi K03243.
OMAi GSLRMHT.
OrthoDBi EOG73BVNC.

Enzyme and pathway databases

BioCyci YEAST:G3O-28845-MONOMER.

Miscellaneous databases

NextBioi 968046.
PROi P39730.

Gene expression databases

Genevestigatori P39730.

Family and domain databases

Gene3Di 3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR000795. EF_GTP-bd_dom.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
Pfami PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Vo D.
    Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
    Strain: ATCC 204511 / S288c / AB972.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Isolation, identification and characterization of the FUN12 gene of Saccharomyces cerevisiae."
    Sutrave P., Shafer B.K., Strathern J.N., Hughes S.H.
    Gene 146:209-213(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "Promotion of met-tRNAiMet binding to ribosomes by yIF2, a bacterial IF2 homolog in yeast."
    Choi S.K., Lee J.H., Zoll W.L., Merrick W.C., Dever T.E.
    Science 280:1757-1760(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-405, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIF2P_YEAST
AccessioniPrimary (citable) accession number: P39730
Secondary accession number(s): D6VPI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 13400 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

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