P39727 (ERV46_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ER-derived vesicles protein ERV46

Gene names

Name:	ERV46
Ordered Locus Names:	YAL042W
ORF Names:	FUN9

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Constituent of COPII-coated endoplasmic reticulum-derived transport vesicles. Required for efficient transport of a subset of secretory proteins to the Golgi. The C-terminal Phe-Tyr motif is required for exit from the endoplasmic reticulum. Facilitates retrograde transport from the Golgi to the endoplasmic reticulum. Ref.5 Ref.6
Subunit structure	Interacts with ERV41. Ref.4 Ref.5
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note: Recycles between endoplasmic reticulum and Golgi. Resides in the endoplasmic and Golgi compartments, and then packaged into endoplasmic reticulum derived vesicles. Ref.4 Ref.5 Ref.6 Ref.7
Sequence similarities	Belongs to the ERGIC family.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ER to Golgi vesicle-mediated transport Inferred from physical interaction Ref.5. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ER to Golgi transport vesicle Inferred from direct assay Ref.5. Source: SGD integral to Golgi membrane Inferred from direct assay Ref.5. Source: SGD integral to endoplasmic reticulum membrane Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ERV41	Q04651	1	EBI-20659,EBI-27850
SPC1	P46965	1	EBI-20659,EBI-17823

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	ER-derived vesicles protein ERV46		PRO_0000202419
Regions
	Topological domain	1 – 24	24	Cytoplasmic Potential
	Transmembrane	25 – 45	21	Helical; Potential
	Topological domain	46 – 376	331	Lumenal Potential
	Transmembrane	377 – 397	21	Helical; Potential
	Topological domain	398 – 415	18	Cytoplasmic Potential
	Motif	402 – 403	2	Phenylalanine-tyrosine motif

Sequences

	Sequence		Length	Mass (Da)
	P39727 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: FB7BC171B88BB5EA	FASTA	415	46,235
	10 20 30 40 50 60 MKRSTLLSLD AFAKTEEDVR VRTRAGGLIT LSCILTTLFL LVNEWGQFNS VVTRPQLVVD 70 80 90 100 110 120 RDRHAKLELN MDVTFPSMPC DLVNLDIMDD SGEMQLDILD AGFTMSRLNS EGRPVGDATE 130 140 150 160 170 180 LHVGGNGDGT APVNNDPNYC GPCYGAKDQS QNENLAQEEK VCCQDCDAVR SAYLEAGWAF 190 200 210 220 230 240 FDGKNIEQCE REGYVSKINE HLNEGCRIKG SAQINRIQGN LHFAPGKPYQ NAYGHFHDTS 250 260 270 280 290 300 LYDKTSNLNF NHIINHLSFG KPIQSHSKLL GNDKRHGGAV VATSPLDGRQ VFPDRNTHFH 310 320 330 340 350 360 QFSYFAKIVP TRYEYLDNVV IETAQFSATF HSRPLAGGRD KDHPNTLHVR GGIPGMFVFF 370 380 390 400 410 EMSPLKVINK EQHGQTWSGF ILNCITSIGG VLAVGTVMDK LFYKAQRSIW GKKSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Vo D. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 405.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A novel membrane protein complex on the endoplasmic reticulum and early Golgi compartments in the yeast Saccharomyces cerevisiae." Cho J.-H., Noda Y., Adachi H., Yoda K. Biosci. Biotechnol. Biochem. 65:2226-2232(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ERV41, SUBCELLULAR LOCATION.
[5]	"Erv41p and Erv46p: new components of COPII vesicles involved in transport between the ER and Golgi complex." Otte S., Belden W.J., Heidtman M., Liu J., Jensen O.N., Barlowe C. J. Cell Biol. 152:503-518(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ERV41, SUBCELLULAR LOCATION.
[6]	"The Erv41p-Erv46p complex: multiple export signals are required in trans for COPII-dependent transport from the ER." Otte S., Barlowe C. EMBO J. 21:6095-6104(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[7]	"Immunoisolaton of the yeast Golgi subcompartments and characterization of a novel membrane protein, Svp26, discovered in the Sed5-containing compartments." Inadome H., Noda Y., Adachi H., Yoda K. Mol. Cell. Biol. 25:7696-7710(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12980 Genomic DNA. Translation: AAC04989.1. BK006935 Genomic DNA. Translation: DAA06944.1.
PIR	S51977.
RefSeq	NP_009358.1. NM_001178187.1.
3D structure databases
ProteinModelPortal	P39727.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3792N.
IntAct	P39727. 4 interactions.
MINT	MINT-565838.
STRING	4932.YAL042W.
Proteomic databases
PaxDb	P39727.
PeptideAtlas	P39727.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL042W; YAL042W; YAL042W.
GeneID	851256.
KEGG	sce:YAL042W.
Organism-specific databases
CYGD	YAL042w.
SGD	S000000040. ERV46.
Phylogenomic databases
eggNOG	NOG260258.
GeneTree	ENSGT00530000063113.
HOGENOM	HOG000210954.
OMA	HEKSANG.
OrthoDB	EOG4ZPJ43.
Gene expression databases
Genevestigator	P39727.
GermOnline	YAL042W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR027198. Erv41/Erv46. IPR012936. Erv_C. [Graphical view]
PANTHER	PTHR10984:SF3. PTHR10984:SF3. 1 hit.
Pfam	PF07970. COPIIcoated_ERV. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968210.

Entry information

Entry name

ERV46_YEAST

Accession

Primary (citable) accession number: P39727
Secondary accession number(s): D6VPH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program