Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P39726 (GCSH_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycine cleavage system H protein, mitochondrial
Alternative name(s):
Glycine decarboxylase complex subunit H
Gene names
Name:GCV3
Ordered Locus Names:YAL044C
ORF Names:FUN40
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length170 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The glycine cleavage system (glycine decarboxylase complex) catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein By similarity. Ref.3

Cofactor

Binds 1 lipoyl cofactor covalently By similarity. HAMAP-Rule MF_00272

Subunit structure

Component of the glycine decarboxylase complex (GDC), which is composed of four proteins: P, T, L and H.

Subcellular location

Mitochondrion Ref.5 Ref.7.

Induction

Induced by glycine and repressed by the C1 metabolic end products. Ref.3

Miscellaneous

Present with 3510 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the GcvH family.

Contains 1 lipoyl-binding domain.

Sequence caution

The sequence AAC04987.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-7486,EBI-7486

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4747Mitochondrion Potential
Chain48 – 170123Glycine cleavage system H protein, mitochondrial HAMAP-Rule MF_00272
PRO_0000010739

Amino acid modifications

Modified residue1021N6-lipoyllysine By similarity

Experimental info

Sequence conflict141T → A in AAC04987. Ref.1
Sequence conflict731A → S in AAC04987. Ref.1
Sequence conflict901A → S in AAC04987. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P39726 [UniParc].

Last modified November 2, 2010. Version 3.
Checksum: 6DC35B6CCC71A6D6

FASTA17018,793
        10         20         30         40         50         60 
MLRTTRLWTT RMPTVSKLFL RNSSGNALNK NKLPFLYSSQ GPQAVRYTSQ HEWIAVHQDK 

        70         80         90        100        110        120 
TAFVGITKYA TDALGDATYV ELPEVGTEIA QGESLGSIES VKSASEIYQP ADGTVEEINT 

       130        140        150        160        170 
NLEENPGVVN EDPMGDGWLV KMKLGEGVNV EQVEGLMSLE QYEKTLVHDD 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 14; 73 AND 90.
Strain: ATCC 204508 / S288c.
[3]"Molecular characterization of GCV3, the Saccharomyces cerevisiae gene coding for the glycine cleavage system hydrogen carrier protein."
Nagarajan L., Storms R.K.
J. Biol. Chem. 272:4444-4450(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
[4]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12980 Genomic DNA. Translation: AAC04987.1. Different initiation.
BK006935 Genomic DNA. Translation: DAA06942.1.
PIRS51975.
RefSeqNP_009355.3. NM_001178189.1.

3D structure databases

ProteinModelPortalP39726.
SMRP39726. Positions 42-143.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31783. 44 interactions.
IntActP39726. 1 interaction.
MINTMINT-669758.
STRING4932.YAL044C.

Proteomic databases

MaxQBP39726.
PaxDbP39726.
PeptideAtlasP39726.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAL044C; YAL044C; YAL044C.
GeneID851254.
KEGGsce:YAL044C.

Organism-specific databases

CYGDYAL044c.
SGDS000000042. GCV3.

Phylogenomic databases

eggNOGCOG0509.
GeneTreeENSGT00390000011666.
HOGENOMHOG000239392.
KOK02437.
OMAHEHTWAK.
OrthoDBEOG7SR4ZT.

Enzyme and pathway databases

BioCycYEAST:G3O-28852-MONOMER.
YEAST:GCVH-MONOMER.

Gene expression databases

GenevestigatorP39726.

Family and domain databases

HAMAPMF_00272. GcvH.
InterProIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR002930. GCV_H.
IPR017453. GCV_H_sub.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERPTHR11715. PTHR11715. 1 hit.
PfamPF01597. GCV_H. 1 hit.
[Graphical view]
SUPFAMSSF51230. SSF51230. 1 hit.
TIGRFAMsTIGR00527. gcvH. 1 hit.
PROSITEPS00189. LIPOYL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968204.
PROP39726.

Entry information

Entry nameGCSH_YEAST
AccessionPrimary (citable) accession number: P39726
Secondary accession number(s): D6VPH2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 2, 2010
Last modified: May 14, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families