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P39726

- GCSH_YEAST

UniProt

P39726 - GCSH_YEAST

Protein

Glycine cleavage system H protein, mitochondrial

Gene

GCV3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 3 (02 Nov 2010)
      Previous versions | rss
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    Functioni

    The glycine cleavage system (glycine decarboxylase complex) catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein By similarity.By similarity

    Cofactori

    Binds 1 lipoyl cofactor covalently.By similarity

    GO - Biological processi

    1. glycine catabolic process Source: SGD
    2. glycine decarboxylation via glycine cleavage system Source: InterPro
    3. one-carbon metabolic process Source: SGD
    4. oxidation-reduction process Source: GOC
    5. protein lipoylation Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28852-MONOMER.
    YEAST:GCVH-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycine cleavage system H protein, mitochondrial
    Alternative name(s):
    Glycine decarboxylase complex subunit H
    Gene namesi
    Name:GCV3
    Ordered Locus Names:YAL044C
    ORF Names:FUN40
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAL044c.
    SGDiS000000042. GCV3.

    Subcellular locationi

    Mitochondrion 2 Publications

    GO - Cellular componenti

    1. glycine cleavage complex Source: SGD
    2. mitochondrion Source: SGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4747MitochondrionSequence AnalysisAdd
    BLAST
    Chaini48 – 170123Glycine cleavage system H protein, mitochondrialPRO_0000010739Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei102 – 1021N6-lipoyllysineBy similarity

    Proteomic databases

    MaxQBiP39726.
    PaxDbiP39726.
    PeptideAtlasiP39726.

    Expressioni

    Inductioni

    Induced by glycine and repressed by the C1 metabolic end products.1 Publication

    Gene expression databases

    GenevestigatoriP39726.

    Interactioni

    Subunit structurei

    Component of the glycine decarboxylase complex (GDC), which is composed of four proteins: P, T, L and H.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-7486,EBI-7486

    Protein-protein interaction databases

    BioGridi31783. 44 interactions.
    IntActiP39726. 1 interaction.
    MINTiMINT-669758.
    STRINGi4932.YAL044C.

    Structurei

    3D structure databases

    ProteinModelPortaliP39726.
    SMRiP39726. Positions 42-143.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GcvH family.Curated
    Contains 1 lipoyl-binding domain.Curated

    Keywords - Domaini

    Lipoyl, Transit peptide

    Phylogenomic databases

    eggNOGiCOG0509.
    GeneTreeiENSGT00390000011666.
    HOGENOMiHOG000239392.
    KOiK02437.
    OMAiHEHTWAK.
    OrthoDBiEOG7SR4ZT.

    Family and domain databases

    HAMAPiMF_00272. GcvH.
    InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
    IPR002930. GCV_H.
    IPR017453. GCV_H_sub.
    IPR011053. Single_hybrid_motif.
    [Graphical view]
    PANTHERiPTHR11715. PTHR11715. 1 hit.
    PfamiPF01597. GCV_H. 1 hit.
    [Graphical view]
    SUPFAMiSSF51230. SSF51230. 1 hit.
    TIGRFAMsiTIGR00527. gcvH. 1 hit.
    PROSITEiPS00189. LIPOYL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P39726-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRTTRLWTT RMPTVSKLFL RNSSGNALNK NKLPFLYSSQ GPQAVRYTSQ    50
    HEWIAVHQDK TAFVGITKYA TDALGDATYV ELPEVGTEIA QGESLGSIES 100
    VKSASEIYQP ADGTVEEINT NLEENPGVVN EDPMGDGWLV KMKLGEGVNV 150
    EQVEGLMSLE QYEKTLVHDD 170
    Length:170
    Mass (Da):18,793
    Last modified:November 2, 2010 - v3
    Checksum:i6DC35B6CCC71A6D6
    GO

    Sequence cautioni

    The sequence AAC04987.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti14 – 141T → A in AAC04987. (PubMed:7731988)Curated
    Sequence conflicti73 – 731A → S in AAC04987. (PubMed:7731988)Curated
    Sequence conflicti90 – 901A → S in AAC04987. (PubMed:7731988)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12980 Genomic DNA. Translation: AAC04987.1. Different initiation.
    BK006935 Genomic DNA. Translation: DAA06942.1.
    PIRiS51975.
    RefSeqiNP_009355.3. NM_001178189.1.

    Genome annotation databases

    EnsemblFungiiYAL044C; YAL044C; YAL044C.
    GeneIDi851254.
    KEGGisce:YAL044C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12980 Genomic DNA. Translation: AAC04987.1 . Different initiation.
    BK006935 Genomic DNA. Translation: DAA06942.1 .
    PIRi S51975.
    RefSeqi NP_009355.3. NM_001178189.1.

    3D structure databases

    ProteinModelPortali P39726.
    SMRi P39726. Positions 42-143.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31783. 44 interactions.
    IntActi P39726. 1 interaction.
    MINTi MINT-669758.
    STRINGi 4932.YAL044C.

    Proteomic databases

    MaxQBi P39726.
    PaxDbi P39726.
    PeptideAtlasi P39726.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL044C ; YAL044C ; YAL044C .
    GeneIDi 851254.
    KEGGi sce:YAL044C.

    Organism-specific databases

    CYGDi YAL044c.
    SGDi S000000042. GCV3.

    Phylogenomic databases

    eggNOGi COG0509.
    GeneTreei ENSGT00390000011666.
    HOGENOMi HOG000239392.
    KOi K02437.
    OMAi HEHTWAK.
    OrthoDBi EOG7SR4ZT.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28852-MONOMER.
    YEAST:GCVH-MONOMER.

    Miscellaneous databases

    NextBioi 968204.
    PROi P39726.

    Gene expression databases

    Genevestigatori P39726.

    Family and domain databases

    HAMAPi MF_00272. GcvH.
    InterProi IPR003016. 2-oxoA_DH_lipoyl-BS.
    IPR002930. GCV_H.
    IPR017453. GCV_H_sub.
    IPR011053. Single_hybrid_motif.
    [Graphical view ]
    PANTHERi PTHR11715. PTHR11715. 1 hit.
    Pfami PF01597. GCV_H. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51230. SSF51230. 1 hit.
    TIGRFAMsi TIGR00527. gcvH. 1 hit.
    PROSITEi PS00189. LIPOYL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 14; 73 AND 90.
      Strain: ATCC 204508 / S288c.
    3. "Molecular characterization of GCV3, the Saccharomyces cerevisiae gene coding for the glycine cleavage system hydrogen carrier protein."
      Nagarajan L., Storms R.K.
      J. Biol. Chem. 272:4444-4450(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    4. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGCSH_YEAST
    AccessioniPrimary (citable) accession number: P39726
    Secondary accession number(s): D6VPH2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 2, 2010
    Last modified: October 1, 2014
    This is version 126 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3510 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3