P39726 (GCSH_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine cleavage system H protein, mitochondrial

Alternative name(s):
Glycine decarboxylase complex subunit H

Gene names

Name:	GCV3
Ordered Locus Names:	YAL044C
ORF Names:	FUN40

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The glycine cleavage system (glycine decarboxylase complex) catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein By similarity. Ref.3
Cofactor	Binds 1 lipoyl cofactor covalently By similarity.
Subunit structure	Component of the glycine decarboxylase complex (GDC), which is composed of four proteins: P, T, L and H.
Subcellular location	Mitochondrion Ref.5 Ref.7.
Induction	Induced by glycine and repressed by the C1 metabolic end products. Ref.3
Miscellaneous	Present with 3510 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the GcvH family. Contains 1 lipoyl-binding domain.
Sequence caution	The sequence AAC04987.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Lipoyl Transit peptide
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycine catabolic process Inferred from mutant phenotype Ref.3. Source: SGD glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from genetic interaction PubMed 10871621. Source: SGD protein lipoylation Inferred from mutant phenotype PubMed 19570983. Source: SGD
Cellular_component	glycine cleavage complex Inferred from sequence alignment Ref.3. Source: SGD mitochondrion Inferred from direct assay Ref.7PubMed 16823961PubMed 19570983. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-7486,EBI-7486

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 47	47	Mitochondrion Potential
	Chain	48 – 170	123	Glycine cleavage system H protein, mitochondrial		PRO_0000010739
Amino acid modifications
	Modified residue	39	1	Phosphoserine Ref.8
	Modified residue	102	1	N6-lipoyllysine By similarity
Experimental info
	Sequence conflict	14	1	T → A in AAC04987. Ref.1
	Sequence conflict	73	1	A → S in AAC04987. Ref.1
	Sequence conflict	90	1	A → S in AAC04987. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P39726 [UniParc]. Last modified November 2, 2010. Version 3. Checksum: 6DC35B6CCC71A6D6	FASTA	170	18,793
	10 20 30 40 50 60 MLRTTRLWTT RMPTVSKLFL RNSSGNALNK NKLPFLYSSQ GPQAVRYTSQ HEWIAVHQDK 70 80 90 100 110 120 TAFVGITKYA TDALGDATYV ELPEVGTEIA QGESLGSIES VKSASEIYQP ADGTVEEINT 130 140 150 160 170 NLEENPGVVN EDPMGDGWLV KMKLGEGVNV EQVEGLMSLE QYEKTLVHDD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 14; 73 AND 90. Strain: ATCC 204508 / S288c.
[3]	"Molecular characterization of GCV3, the Saccharomyces cerevisiae gene coding for the glycine cleavage system hydrogen carrier protein." Nagarajan L., Storms R.K. J. Biol. Chem. 272:4444-4450(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[4]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12980 Genomic DNA. Translation: AAC04987.1. Different initiation. BK006935 Genomic DNA. Translation: DAA06942.1.
PIR	S51975.
RefSeq	NP_009355.3. NM_001178189.1.
3D structure databases
ProteinModelPortal	P39726.
SMR	P39726. Positions 42-143.
ModBase	Search...
Protein-protein interaction databases
IntAct	P39726. 1 interaction.
MINT	MINT-669758.
STRING	4932.YAL044C.
Proteomic databases
PaxDb	P39726.
PeptideAtlas	P39726.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL044C; YAL044C; YAL044C.
GeneID	851254.
KEGG	sce:YAL044C.
Organism-specific databases
CYGD	YAL044c.
SGD	S000000042. GCV3.
Phylogenomic databases
eggNOG	COG0509.
GeneTree	ENSGT00390000011666.
HOGENOM	HOG000239392.
KO	K02437.
OMA	EGWIAQI.
OrthoDB	EOG4PVS81.
Gene expression databases
Genevestigator	P39726.
GermOnline	YAL044C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003016. 2-oxoA_DH_lipoyl-BS. IPR002930. GCV_H. IPR017453. GCV_H_sub. IPR011053. Single_hybrid_motif. [Graphical view]
PANTHER	PTHR11715. PTHR11715. 1 hit.
Pfam	PF01597. GCV_H. 1 hit. [Graphical view]
SUPFAM	SSF51230. Hybrid_motif. 1 hit.
TIGRFAMs	TIGR00527. gcvH. 1 hit.
PROSITE	PS00189. LIPOYL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968204.

Entry information

Entry name

GCSH_YEAST

Accession

Primary (citable) accession number: P39726
Secondary accession number(s): D6VPH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 2, 2010
Last modified:	May 1, 2013
This is version 117 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program