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Protein

Glycine cleavage system H protein, mitochondrial

Gene

GCV3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The glycine cleavage system (glycine decarboxylase complex) catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein (By similarity).By similarity1 Publication

Miscellaneous

Present with 3510 molecules/cell in log phase SD medium.1 Publication

Cofactori

(R)-lipoateBy similarityNote: Binds 1 lipoyl cofactor covalently.By similarity

GO - Biological processi

  • glycine catabolic process Source: SGD
  • glycine decarboxylation via glycine cleavage system Source: InterPro
  • one-carbon metabolic process Source: SGD
  • oxidation-reduction process Source: GOC
  • protein lipoylation Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-28852-MONOMER
ReactomeiR-SCE-389661 Glyoxylate metabolism and glycine degradation
R-SCE-6783984 Glycine degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine cleavage system H protein, mitochondrial
Alternative name(s):
Glycine decarboxylase complex subunit H
Gene namesi
Name:GCV3
Ordered Locus Names:YAL044C
ORF Names:FUN40
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL044C
SGDiS000000042 GCV3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 47MitochondrionSequence analysisAdd BLAST47
ChainiPRO_000001073948 – 170Glycine cleavage system H protein, mitochondrialAdd BLAST123

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102N6-lipoyllysinePROSITE-ProRule annotationBy similarity1

Proteomic databases

MaxQBiP39726
PaxDbiP39726
PRIDEiP39726

PTM databases

iPTMnetiP39726

Expressioni

Inductioni

Induced by glycine and repressed by the C1 metabolic end products.1 Publication

Interactioni

Subunit structurei

Component of the glycine decarboxylase complex (GDC), which is composed of four proteins: P, T, L and H.

Protein-protein interaction databases

BioGridi3178387 interactors.
IntActiP39726 3 interactors.
MINTiP39726
STRINGi4932.YAL044C

Structurei

3D structure databases

ProteinModelPortaliP39726
SMRiP39726
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 143Lipoyl-bindingPROSITE-ProRule annotationAdd BLAST83

Sequence similaritiesi

Belongs to the GcvH family.Curated

Keywords - Domaini

Lipoyl, Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000011666
HOGENOMiHOG000239392
InParanoidiP39726
KOiK02437
OMAiIVYVEVE
OrthoDBiEOG092C4XAN

Family and domain databases

CDDicd06848 GCS_H, 1 hit
HAMAPiMF_00272 GcvH, 1 hit
InterProiView protein in InterPro
IPR003016 2-oxoA_DH_lipoyl-BS
IPR000089 Biotin_lipoyl
IPR002930 GCV_H
IPR033753 GCV_H/Fam206
IPR017453 GCV_H_sub
IPR011053 Single_hybrid_motif
PANTHERiPTHR11715 PTHR11715, 1 hit
PfamiView protein in Pfam
PF01597 GCV_H, 1 hit
SUPFAMiSSF51230 SSF51230, 1 hit
TIGRFAMsiTIGR00527 gcvH, 1 hit
PROSITEiView protein in PROSITE
PS50968 BIOTINYL_LIPOYL, 1 hit
PS00189 LIPOYL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRTTRLWTT RMPTVSKLFL RNSSGNALNK NKLPFLYSSQ GPQAVRYTSQ
60 70 80 90 100
HEWIAVHQDK TAFVGITKYA TDALGDATYV ELPEVGTEIA QGESLGSIES
110 120 130 140 150
VKSASEIYQP ADGTVEEINT NLEENPGVVN EDPMGDGWLV KMKLGEGVNV
160 170
EQVEGLMSLE QYEKTLVHDD
Length:170
Mass (Da):18,793
Last modified:November 2, 2010 - v3
Checksum:i6DC35B6CCC71A6D6
GO

Sequence cautioni

The sequence AAC04987 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14T → A in AAC04987 (PubMed:7731988).Curated1
Sequence conflicti73A → S in AAC04987 (PubMed:7731988).Curated1
Sequence conflicti90A → S in AAC04987 (PubMed:7731988).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA Translation: AAC04987.1 Different initiation.
BK006935 Genomic DNA Translation: DAA06942.1
PIRiS51975
RefSeqiNP_009355.3, NM_001178189.1

Genome annotation databases

EnsemblFungiiYAL044C; YAL044C; YAL044C
GeneIDi851254
KEGGisce:YAL044C

Similar proteinsi

Entry informationi

Entry nameiGCSH_YEAST
AccessioniPrimary (citable) accession number: P39726
Secondary accession number(s): D6VPH2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 2, 2010
Last modified: March 28, 2018
This is version 159 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome