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Protein

BolA-like protein 3

Gene

BOL3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a mitochondrial iron-sulfur (Fe-S) cluster assembly factor that facilitates [4Fe-4S] cluster insertion into a subset of mitochondrial proteins such as LIAS and succinate dehydrogenase (SDH) (PubMed:27532773, PubMed:27532772). Required during the last step of iron-sulfur protein assembly when the iron-sulfur cluster is inserted into the target protein (PubMed:27532772). Acts together with NFU1, later than BOL1 and GRX5 in the [4Fe-4S] cluster insertion process (PubMed:27532773). Not required for [2Fe-2S] cluster insertion into mitochondrial proteins (PubMed:27532772).2 Publications

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • protein maturation by iron-sulfur cluster transfer Source: SGD

Enzyme and pathway databases

BioCyciYEAST:G3O-28853-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
BolA-like protein 3Curated
Alternative name(s):
Altered inheritance of mitochondria protein 1
Gene namesi
Name:BOL32 Publications
Synonyms:AIM1
Ordered Locus Names:YAL046C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL046C.
SGDiS000000044. BOL3.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: SGD

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Increases frequency of mitochondrial genome loss (PubMed:19300474). Cells show a slight decrease of succinate dehydrogenase (SDH) (PubMed:27532772). Cells lacking BOL1 and BOL3 display defects in a subset of mitochondrial [4Fe-4S] enzymes (PubMed:27532773, PubMed:27532772).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi64C → A: Partial loss of function. 1 Publication1
Mutagenesisi101H → A: Loss of function. 1 Publication1
Mutagenesisi101H → C: Dominant negative mutant; displays respiratory defects that are higher than the BOL1 BOL3 double mutant. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002012391 – 118BolA-like protein 3Add BLAST118

Proteomic databases

MaxQBiP39724.
PRIDEiP39724.

Interactioni

Subunit structurei

Interacts with NFU1 (PubMed:27532773).1 Publication

Protein-protein interaction databases

BioGridi31781. 100 interactors.
IntActiP39724. 3 interactors.
MINTiMINT-2492096.

Structurei

3D structure databases

ProteinModelPortaliP39724.
SMRiP39724.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the BolA/IbaG family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000013048.
InParanoidiP39724.
OMAiQVESAKF.
OrthoDBiEOG092C5RHO.

Family and domain databases

InterProiView protein in InterPro
IPR002634. BolA.
PANTHERiPTHR12735. PTHR12735. 1 hit.
PfamiView protein in Pfam
PF01722. BolA. 1 hit.
SUPFAMiSSF82657. SSF82657. 1 hit.

Sequencei

Sequence statusi: Complete.

P39724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLPQTMLRS ISVKHVRWPR ILTGSKLWYS TQMAMTPEEK MITDKLQQEL
60 70 80 90 100
EPEVCKVQDV SGGCGSMFAI NITSKKFNGL SLIKQHQLVN RILRDDISRW
110
HGLQLTTKKS TGKGPASS
Length:118
Mass (Da):13,356
Last modified:February 1, 1995 - v1
Checksum:i244F5FF2052FF410
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. Translation: AAC04985.1.
BK006935 Genomic DNA. Translation: DAA06940.1.
PIRiS51973.
RefSeqiNP_009353.1. NM_001178191.1.

Genome annotation databases

EnsemblFungiiYAL046C; YAL046C; YAL046C.
GeneIDi851251.
KEGGisce:YAL046C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. Translation: AAC04985.1.
BK006935 Genomic DNA. Translation: DAA06940.1.
PIRiS51973.
RefSeqiNP_009353.1. NM_001178191.1.

3D structure databases

ProteinModelPortaliP39724.
SMRiP39724.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31781. 100 interactors.
IntActiP39724. 3 interactors.
MINTiMINT-2492096.

Proteomic databases

MaxQBiP39724.
PRIDEiP39724.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL046C; YAL046C; YAL046C.
GeneIDi851251.
KEGGisce:YAL046C.

Organism-specific databases

EuPathDBiFungiDB:YAL046C.
SGDiS000000044. BOL3.

Phylogenomic databases

GeneTreeiENSGT00390000013048.
InParanoidiP39724.
OMAiQVESAKF.
OrthoDBiEOG092C5RHO.

Enzyme and pathway databases

BioCyciYEAST:G3O-28853-MONOMER.

Miscellaneous databases

PROiPR:P39724.

Family and domain databases

InterProiView protein in InterPro
IPR002634. BolA.
PANTHERiPTHR12735. PTHR12735. 1 hit.
PfamiView protein in Pfam
PF01722. BolA. 1 hit.
SUPFAMiSSF82657. SSF82657. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBOL3_YEAST
AccessioniPrimary (citable) accession number: P39724
Secondary accession number(s): D6VPH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 10, 2017
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.