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P39723 (SPC72_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Spindle pole component SPC72
Gene names
Name:SPC72
Synonyms:LDB4, SPI6
Ordered Locus Names:YAL047C
ORF Names:FUN42
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length622 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Spindle pole body component that acts as the gamma-tubulin complex-binding protein of the SPB outer plaque. Anchors cytoplasmic microtubules at the at the half bridge of the spindle pole body (SPB) and accordingly functions in nuclear position and spindle orientation, including anaphase spindle migration into the bud. Recruits KIN4 kinase to both SPBs when cytoplasmic microtubules are defective. Links cytoplasmic microtubules with spindle orientation checkpoint (SPOC) components and, therefore, could function as part of the sensors of spindle orientation defects. Is strictly required for mating and karyogamy. Ref.4 Ref.6 Ref.7 Ref.8 Ref.12

Subunit structure

Homooligomer. Interacts with CDC5, KAR1, KIN4, SPC97, SPC98, STU2 and TUB4. Ref.4 Ref.6 Ref.8 Ref.11 Ref.12

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body. Note: localised at the outer plaque and the bridge of the spindle pole body. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9

Post-translational modification

Phosphorylated by CDC5. Ref.11 Ref.12

Miscellaneous

Present with 639 molecules/cell in log phase SD medium.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 622622Spindle pole component SPC72
PRO_0000202421

Experimental info

Sequence conflict3021N → I in AAC04984. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P39723 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 33A858B9ADCAD633

FASTA62272,106
        10         20         30         40         50         60 
MVRRWIPSGR HLRNNDNTGD DDDSEFTNSM DSGMSIPSLR DSMTTRSSHN DPIKPALMND 

        70         80         90        100        110        120 
SNKVKNLEKE LTNAKIKIQV LYEYIRRIPN KDGNAPSLGN DTDFRNSIIE GLNLEINKLK 

       130        140        150        160        170        180 
QDLKAKEVEY QDTLQFVQEN LENSESIVNT INHLLSFILT HFNEQDENAH LLDKEERETL 

       190        200        210        220        230        240 
EETLELSSDY VLEKMDTLSK FIIQFLQDFL HSKSRAESKQ DKEEFLSLAQ SSPAGSQLES 

       250        260        270        280        290        300 
RDSPSSKEEN TDGGYQNDEI HDSNNHIDTE NVMANSTSLP ISAVESRFEK TLDTQLEIVI 

       310        320        330        340        350        360 
ENLHKEYDQF INSIRLKFEK SQKLEKIIAS KLNEQSHLLD SLELEENSSS VIEKQDHLIS 

       370        380        390        400        410        420 
QLKEKIESQS VLINNLEKLK EDIIKMKQNE KVLTKELETQ TKINKLKENN WDSYINDLEK 

       430        440        450        460        470        480 
QINDLQIDKS EEFHVIQNQL DKLDLENYQL KNQLNTLDNQ KLILSQYESN FIKFNQNLLL 

       490        500        510        520        530        540 
HLDSIFNILQ KILQESSIAQ FDRKMKSIKS VPNALKNLNL IQPKLESLYT FIETALESII 

       550        560        570        580        590        600 
NSYISSLISM ETPEQPHQQG NELTATPNKE LTLRIEELQR RWISERERRK LDANASEARI 

       610        620 
KALEQENESL RSKLFNLSIN NP 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 302.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation."
Knop M., Schiebel E.
EMBO J. 17:3952-3967(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SPC97; SPC98 AND TUB4.
[5]"Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry."
Wigge P.A., Jensen O.N., Holmes S., Soues S., Mann M., Kilmartin J.V.
J. Cell Biol. 141:967-977(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[6]"The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly."
Chen X.P., Yin H., Huffaker T.C.
J. Cell Biol. 141:1169-1179(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH STU2.
[7]"SPC72: a spindle pole component required for spindle orientation in the yeast Saccharomyces cerevisiae."
Soues S., Adams I.R.
J. Cell Sci. 111:2809-2818(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]"Interaction of the yeast gamma-tubulin complex-binding protein Spc72p with Kar1p is essential for microtubule function during karyogamy."
Pereira G., Grueneberg U., Knop M., Schiebel E.
EMBO J. 18:4180-4195(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KAR1.
[9]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"A coupled chemical-genetic and bioinformatic approach to Polo-like kinase pathway exploration."
Snead J.L., Sullivan M., Lowery D.M., Cohen M.S., Zhang C., Randle D.H., Taunton J., Yaffe M.B., Morgan D.O., Shokat K.M.
Chem. Biol. 14:1261-1272(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CDC5, PHOSPHORYLATION BY CDC5.
[12]"The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal."
Maekawa H., Priest C., Lechner J., Pereira G., Schiebel E.
J. Cell Biol. 179:423-436(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION BY CDC5, INTERACTION WITH KIN4.
[13]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12980 Genomic DNA. Translation: AAC04984.1.
AY723757 Genomic DNA. Translation: AAU09674.1.
BK006935 Genomic DNA. Translation: DAA06939.2.
PIRS51972.
RefSeqNP_009352.2. NM_001178192.2.

3D structure databases

ProteinModelPortalP39723.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31780. 125 interactions.
DIPDIP-868N.
IntActP39723. 35 interactions.
MINTMINT-385032.
STRING4932.YAL047C.

Proteomic databases

PaxDbP39723.
PRIDEP39723.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAL047C; YAL047C; YAL047C.
GeneID851250.
KEGGsce:YAL047C.

Organism-specific databases

CYGDYAL047c.
SGDS000000045. SPC72.

Phylogenomic databases

eggNOGNOG129411.
OMANISERDE.
OrthoDBEOG7QC866.

Enzyme and pathway databases

BioCycYEAST:G3O-28854-MONOMER.

Gene expression databases

GenevestigatorP39723.

Family and domain databases

InterProIPR024545. Mto2p-binding.
[Graphical view]
PfamPF12808. Mto2_bdg. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968195.

Entry information

Entry nameSPC72_YEAST
AccessionPrimary (citable) accession number: P39723
Secondary accession number(s): D6VPG9, E9P941
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD