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P39723

- SPC72_YEAST

UniProt

P39723 - SPC72_YEAST

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Protein

Spindle pole component SPC72

Gene

SPC72

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Spindle pole body component that acts as the gamma-tubulin complex-binding protein of the SPB outer plaque. Anchors cytoplasmic microtubules at the at the half bridge of the spindle pole body (SPB) and accordingly functions in nuclear position and spindle orientation, including anaphase spindle migration into the bud. Recruits KIN4 kinase to both SPBs when cytoplasmic microtubules are defective. Links cytoplasmic microtubules with spindle orientation checkpoint (SPOC) components and, therefore, could function as part of the sensors of spindle orientation defects. Is strictly required for mating and karyogamy.5 Publications

GO - Molecular functioni

  1. structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  1. karyogamy Source: UniProtKB-KW
  2. microtubule nucleation Source: SGD
  3. mitotic sister chromatid segregation Source: SGD
  4. mitotic spindle elongation Source: SGD
  5. mitotic spindle orientation checkpoint Source: SGD
  6. nuclear migration along microtubule Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Karyogamy

Enzyme and pathway databases

BioCyciYEAST:G3O-28854-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Spindle pole component SPC72
Gene namesi
Name:SPC72
Synonyms:LDB4, SPI6
Ordered Locus Names:YAL047C
ORF Names:FUN42
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL047c.
SGDiS000000045. SPC72.

Subcellular locationi

Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body 6 Publications
Note: localised at the outer plaque and the bridge of the spindle pole body.

GO - Cellular componenti

  1. outer plaque of spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 622622Spindle pole component SPC72PRO_0000202421Add
BLAST

Post-translational modificationi

Phosphorylated by CDC5.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39723.
PaxDbiP39723.
PRIDEiP39723.

Expressioni

Gene expression databases

GenevestigatoriP39723.

Interactioni

Subunit structurei

Homooligomer. Interacts with CDC5, KAR1, KIN4, SPC97, SPC98, STU2 and TUB4.5 Publications

Protein-protein interaction databases

BioGridi31780. 125 interactions.
DIPiDIP-868N.
IntActiP39723. 35 interactions.
MINTiMINT-385032.
STRINGi4932.YAL047C.

Structurei

3D structure databases

ProteinModelPortaliP39723.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG129411.
InParanoidiP39723.
OrthoDBiEOG7QC866.

Family and domain databases

InterProiIPR024545. Mto2p-binding.
[Graphical view]
PfamiPF12808. Mto2_bdg. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39723-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVRRWIPSGR HLRNNDNTGD DDDSEFTNSM DSGMSIPSLR DSMTTRSSHN
60 70 80 90 100
DPIKPALMND SNKVKNLEKE LTNAKIKIQV LYEYIRRIPN KDGNAPSLGN
110 120 130 140 150
DTDFRNSIIE GLNLEINKLK QDLKAKEVEY QDTLQFVQEN LENSESIVNT
160 170 180 190 200
INHLLSFILT HFNEQDENAH LLDKEERETL EETLELSSDY VLEKMDTLSK
210 220 230 240 250
FIIQFLQDFL HSKSRAESKQ DKEEFLSLAQ SSPAGSQLES RDSPSSKEEN
260 270 280 290 300
TDGGYQNDEI HDSNNHIDTE NVMANSTSLP ISAVESRFEK TLDTQLEIVI
310 320 330 340 350
ENLHKEYDQF INSIRLKFEK SQKLEKIIAS KLNEQSHLLD SLELEENSSS
360 370 380 390 400
VIEKQDHLIS QLKEKIESQS VLINNLEKLK EDIIKMKQNE KVLTKELETQ
410 420 430 440 450
TKINKLKENN WDSYINDLEK QINDLQIDKS EEFHVIQNQL DKLDLENYQL
460 470 480 490 500
KNQLNTLDNQ KLILSQYESN FIKFNQNLLL HLDSIFNILQ KILQESSIAQ
510 520 530 540 550
FDRKMKSIKS VPNALKNLNL IQPKLESLYT FIETALESII NSYISSLISM
560 570 580 590 600
ETPEQPHQQG NELTATPNKE LTLRIEELQR RWISERERRK LDANASEARI
610 620
KALEQENESL RSKLFNLSIN NP
Length:622
Mass (Da):72,106
Last modified:July 27, 2011 - v2
Checksum:i33A858B9ADCAD633
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti302 – 3021N → I in AAC04984. (PubMed:7731988)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12980 Genomic DNA. Translation: AAC04984.1.
AY723757 Genomic DNA. Translation: AAU09674.1.
BK006935 Genomic DNA. Translation: DAA06939.2.
PIRiS51972.
RefSeqiNP_009352.2. NM_001178192.2.

Genome annotation databases

EnsemblFungiiYAL047C; YAL047C; YAL047C.
GeneIDi851250.
KEGGisce:YAL047C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12980 Genomic DNA. Translation: AAC04984.1 .
AY723757 Genomic DNA. Translation: AAU09674.1 .
BK006935 Genomic DNA. Translation: DAA06939.2 .
PIRi S51972.
RefSeqi NP_009352.2. NM_001178192.2.

3D structure databases

ProteinModelPortali P39723.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31780. 125 interactions.
DIPi DIP-868N.
IntActi P39723. 35 interactions.
MINTi MINT-385032.
STRINGi 4932.YAL047C.

Proteomic databases

MaxQBi P39723.
PaxDbi P39723.
PRIDEi P39723.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL047C ; YAL047C ; YAL047C .
GeneIDi 851250.
KEGGi sce:YAL047C.

Organism-specific databases

CYGDi YAL047c.
SGDi S000000045. SPC72.

Phylogenomic databases

eggNOGi NOG129411.
InParanoidi P39723.
OrthoDBi EOG7QC866.

Enzyme and pathway databases

BioCyci YEAST:G3O-28854-MONOMER.

Miscellaneous databases

NextBioi 968195.

Gene expression databases

Genevestigatori P39723.

Family and domain databases

InterProi IPR024545. Mto2p-binding.
[Graphical view ]
Pfami PF12808. Mto2_bdg. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 302.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation."
    Knop M., Schiebel E.
    EMBO J. 17:3952-3967(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SPC97; SPC98 AND TUB4.
  5. "Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry."
    Wigge P.A., Jensen O.N., Holmes S., Soues S., Mann M., Kilmartin J.V.
    J. Cell Biol. 141:967-977(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly."
    Chen X.P., Yin H., Huffaker T.C.
    J. Cell Biol. 141:1169-1179(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH STU2.
  7. "SPC72: a spindle pole component required for spindle orientation in the yeast Saccharomyces cerevisiae."
    Soues S., Adams I.R.
    J. Cell Sci. 111:2809-2818(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "Interaction of the yeast gamma-tubulin complex-binding protein Spc72p with Kar1p is essential for microtubule function during karyogamy."
    Pereira G., Grueneberg U., Knop M., Schiebel E.
    EMBO J. 18:4180-4195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KAR1.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "A coupled chemical-genetic and bioinformatic approach to Polo-like kinase pathway exploration."
    Snead J.L., Sullivan M., Lowery D.M., Cohen M.S., Zhang C., Randle D.H., Taunton J., Yaffe M.B., Morgan D.O., Shokat K.M.
    Chem. Biol. 14:1261-1272(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDC5, PHOSPHORYLATION BY CDC5.
  12. "The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal."
    Maekawa H., Priest C., Lechner J., Pereira G., Schiebel E.
    J. Cell Biol. 179:423-436(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION BY CDC5, INTERACTION WITH KIN4.
  13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSPC72_YEAST
AccessioniPrimary (citable) accession number: P39723
Secondary accession number(s): D6VPG9, E9P941
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 639 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3