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P39723

- SPC72_YEAST

UniProt

P39723 - SPC72_YEAST

Protein

Spindle pole component SPC72

Gene

SPC72

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Spindle pole body component that acts as the gamma-tubulin complex-binding protein of the SPB outer plaque. Anchors cytoplasmic microtubules at the at the half bridge of the spindle pole body (SPB) and accordingly functions in nuclear position and spindle orientation, including anaphase spindle migration into the bud. Recruits KIN4 kinase to both SPBs when cytoplasmic microtubules are defective. Links cytoplasmic microtubules with spindle orientation checkpoint (SPOC) components and, therefore, could function as part of the sensors of spindle orientation defects. Is strictly required for mating and karyogamy.5 Publications

    GO - Molecular functioni

    1. structural constituent of cytoskeleton Source: SGD

    GO - Biological processi

    1. karyogamy Source: UniProtKB-KW
    2. microtubule nucleation Source: SGD
    3. mitotic sister chromatid segregation Source: SGD
    4. mitotic spindle elongation Source: SGD
    5. mitotic spindle orientation checkpoint Source: SGD
    6. nuclear migration along microtubule Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Karyogamy

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28854-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Spindle pole component SPC72
    Gene namesi
    Name:SPC72
    Synonyms:LDB4, SPI6
    Ordered Locus Names:YAL047C
    ORF Names:FUN42
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAL047c.
    SGDiS000000045. SPC72.

    Subcellular locationi

    Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body 6 Publications
    Note: localised at the outer plaque and the bridge of the spindle pole body.

    GO - Cellular componenti

    1. outer plaque of spindle pole body Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Spindle pole component SPC72PRO_0000202421Add
    BLAST

    Post-translational modificationi

    Phosphorylated by CDC5.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39723.
    PaxDbiP39723.
    PRIDEiP39723.

    Expressioni

    Gene expression databases

    GenevestigatoriP39723.

    Interactioni

    Subunit structurei

    Homooligomer. Interacts with CDC5, KAR1, KIN4, SPC97, SPC98, STU2 and TUB4.5 Publications

    Protein-protein interaction databases

    BioGridi31780. 125 interactions.
    DIPiDIP-868N.
    IntActiP39723. 35 interactions.
    MINTiMINT-385032.
    STRINGi4932.YAL047C.

    Structurei

    3D structure databases

    ProteinModelPortaliP39723.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiNOG129411.
    OrthoDBiEOG7QC866.

    Family and domain databases

    InterProiIPR024545. Mto2p-binding.
    [Graphical view]
    PfamiPF12808. Mto2_bdg. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39723-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVRRWIPSGR HLRNNDNTGD DDDSEFTNSM DSGMSIPSLR DSMTTRSSHN    50
    DPIKPALMND SNKVKNLEKE LTNAKIKIQV LYEYIRRIPN KDGNAPSLGN 100
    DTDFRNSIIE GLNLEINKLK QDLKAKEVEY QDTLQFVQEN LENSESIVNT 150
    INHLLSFILT HFNEQDENAH LLDKEERETL EETLELSSDY VLEKMDTLSK 200
    FIIQFLQDFL HSKSRAESKQ DKEEFLSLAQ SSPAGSQLES RDSPSSKEEN 250
    TDGGYQNDEI HDSNNHIDTE NVMANSTSLP ISAVESRFEK TLDTQLEIVI 300
    ENLHKEYDQF INSIRLKFEK SQKLEKIIAS KLNEQSHLLD SLELEENSSS 350
    VIEKQDHLIS QLKEKIESQS VLINNLEKLK EDIIKMKQNE KVLTKELETQ 400
    TKINKLKENN WDSYINDLEK QINDLQIDKS EEFHVIQNQL DKLDLENYQL 450
    KNQLNTLDNQ KLILSQYESN FIKFNQNLLL HLDSIFNILQ KILQESSIAQ 500
    FDRKMKSIKS VPNALKNLNL IQPKLESLYT FIETALESII NSYISSLISM 550
    ETPEQPHQQG NELTATPNKE LTLRIEELQR RWISERERRK LDANASEARI 600
    KALEQENESL RSKLFNLSIN NP 622
    Length:622
    Mass (Da):72,106
    Last modified:July 27, 2011 - v2
    Checksum:i33A858B9ADCAD633
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti302 – 3021N → I in AAC04984. (PubMed:7731988)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12980 Genomic DNA. Translation: AAC04984.1.
    AY723757 Genomic DNA. Translation: AAU09674.1.
    BK006935 Genomic DNA. Translation: DAA06939.2.
    PIRiS51972.
    RefSeqiNP_009352.2. NM_001178192.2.

    Genome annotation databases

    EnsemblFungiiYAL047C; YAL047C; YAL047C.
    GeneIDi851250.
    KEGGisce:YAL047C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12980 Genomic DNA. Translation: AAC04984.1 .
    AY723757 Genomic DNA. Translation: AAU09674.1 .
    BK006935 Genomic DNA. Translation: DAA06939.2 .
    PIRi S51972.
    RefSeqi NP_009352.2. NM_001178192.2.

    3D structure databases

    ProteinModelPortali P39723.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31780. 125 interactions.
    DIPi DIP-868N.
    IntActi P39723. 35 interactions.
    MINTi MINT-385032.
    STRINGi 4932.YAL047C.

    Proteomic databases

    MaxQBi P39723.
    PaxDbi P39723.
    PRIDEi P39723.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL047C ; YAL047C ; YAL047C .
    GeneIDi 851250.
    KEGGi sce:YAL047C.

    Organism-specific databases

    CYGDi YAL047c.
    SGDi S000000045. SPC72.

    Phylogenomic databases

    eggNOGi NOG129411.
    OrthoDBi EOG7QC866.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28854-MONOMER.

    Miscellaneous databases

    NextBioi 968195.

    Gene expression databases

    Genevestigatori P39723.

    Family and domain databases

    InterProi IPR024545. Mto2p-binding.
    [Graphical view ]
    Pfami PF12808. Mto2_bdg. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 302.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation."
      Knop M., Schiebel E.
      EMBO J. 17:3952-3967(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SPC97; SPC98 AND TUB4.
    5. "Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry."
      Wigge P.A., Jensen O.N., Holmes S., Soues S., Mann M., Kilmartin J.V.
      J. Cell Biol. 141:967-977(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    6. "The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly."
      Chen X.P., Yin H., Huffaker T.C.
      J. Cell Biol. 141:1169-1179(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH STU2.
    7. "SPC72: a spindle pole component required for spindle orientation in the yeast Saccharomyces cerevisiae."
      Soues S., Adams I.R.
      J. Cell Sci. 111:2809-2818(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    8. "Interaction of the yeast gamma-tubulin complex-binding protein Spc72p with Kar1p is essential for microtubule function during karyogamy."
      Pereira G., Grueneberg U., Knop M., Schiebel E.
      EMBO J. 18:4180-4195(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KAR1.
    9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "A coupled chemical-genetic and bioinformatic approach to Polo-like kinase pathway exploration."
      Snead J.L., Sullivan M., Lowery D.M., Cohen M.S., Zhang C., Randle D.H., Taunton J., Yaffe M.B., Morgan D.O., Shokat K.M.
      Chem. Biol. 14:1261-1272(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CDC5, PHOSPHORYLATION BY CDC5.
    12. "The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal."
      Maekawa H., Priest C., Lechner J., Pereira G., Schiebel E.
      J. Cell Biol. 179:423-436(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION BY CDC5, INTERACTION WITH KIN4.
    13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSPC72_YEAST
    AccessioniPrimary (citable) accession number: P39723
    Secondary accession number(s): D6VPG9, E9P941
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 639 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3