P39723 (SPC72_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spindle pole component SPC72

Gene names

Name:	SPC72
Synonyms:	LDB4, SPI6
Ordered Locus Names:	YAL047C
ORF Names:	FUN42

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	622 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Spindle pole body component that acts as the gamma-tubulin complex-binding protein of the SPB outer plaque. Anchors cytoplasmic microtubules at the at the half bridge of the spindle pole body (SPB) and accordingly functions in nuclear position and spindle orientation, including anaphase spindle migration into the bud. Recruits KIN4 kinase to both SPBs when cytoplasmic microtubules are defective. Links cytoplasmic microtubules with spindle orientation checkpoint (SPOC) components and, therefore, could function as part of the sensors of spindle orientation defects. Is strictly required for mating and karyogamy. Ref.4 Ref.6 Ref.7 Ref.8 Ref.12
Subunit structure	Homooligomer. Interacts with CDC5, KAR1, KIN4, SPC97, SPC98, STU2 and TUB4. Ref.4 Ref.6 Ref.8 Ref.11 Ref.12
Subcellular location	Cytoplasm › cytoskeleton › spindle pole body. Note: localised at the outer plaque and the bridge of the spindle pole body. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Post-translational modification	Phosphorylated by CDC5. Ref.11 Ref.12
Miscellaneous	Present with 639 molecules/cell in log phase SD medium.

Ontologies

Keywords
Biological process	Cell cycle Cell division Karyogamy
Cellular component	Cytoplasm Cytoskeleton
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW karyogamy Inferred from electronic annotation. Source: UniProtKB-KW microtubule nucleation Inferred from mutant phenotype PubMed 18573877. Source: SGD mitotic sister chromatid segregation Inferred from mutant phenotype Ref.6. Source: SGD mitotic spindle elongation Inferred from mutant phenotype Ref.4. Source: SGD mitotic spindle orientation checkpoint Inferred from genetic interaction Ref.12. Source: SGD nuclear migration along microtubule Inferred from mutant phenotype Ref.4. Source: SGD
Cellular_component	outer plaque of spindle pole body Inferred from direct assay Ref.4. Source: SGD
Molecular_function	structural constituent of cytoskeleton Inferred from physical interaction Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 622	622	Spindle pole component SPC72		PRO_0000202421
Amino acid modifications
	Modified residue	232	1	Phosphoserine Ref.13
Experimental info
	Sequence conflict	302	1	N → I in AAC04984. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P39723 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 33A858B9ADCAD633	FASTA	622	72,106
	10 20 30 40 50 60 MVRRWIPSGR HLRNNDNTGD DDDSEFTNSM DSGMSIPSLR DSMTTRSSHN DPIKPALMND 70 80 90 100 110 120 SNKVKNLEKE LTNAKIKIQV LYEYIRRIPN KDGNAPSLGN DTDFRNSIIE GLNLEINKLK 130 140 150 160 170 180 QDLKAKEVEY QDTLQFVQEN LENSESIVNT INHLLSFILT HFNEQDENAH LLDKEERETL 190 200 210 220 230 240 EETLELSSDY VLEKMDTLSK FIIQFLQDFL HSKSRAESKQ DKEEFLSLAQ SSPAGSQLES 250 260 270 280 290 300 RDSPSSKEEN TDGGYQNDEI HDSNNHIDTE NVMANSTSLP ISAVESRFEK TLDTQLEIVI 310 320 330 340 350 360 ENLHKEYDQF INSIRLKFEK SQKLEKIIAS KLNEQSHLLD SLELEENSSS VIEKQDHLIS 370 380 390 400 410 420 QLKEKIESQS VLINNLEKLK EDIIKMKQNE KVLTKELETQ TKINKLKENN WDSYINDLEK 430 440 450 460 470 480 QINDLQIDKS EEFHVIQNQL DKLDLENYQL KNQLNTLDNQ KLILSQYESN FIKFNQNLLL 490 500 510 520 530 540 HLDSIFNILQ KILQESSIAQ FDRKMKSIKS VPNALKNLNL IQPKLESLYT FIETALESII 550 560 570 580 590 600 NSYISSLISM ETPEQPHQQG NELTATPNKE LTLRIEELQR RWISERERRK LDANASEARI 610 620 KALEQENESL RSKLFNLSIN NP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 302. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Receptors determine the cellular localization of a gamma-tubulin complex and thereby the site of microtubule formation." Knop M., Schiebel E. EMBO J. 17:3952-3967(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SPC97; SPC98 AND TUB4.
[5]	"Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry." Wigge P.A., Jensen O.N., Holmes S., Soues S., Mann M., Kilmartin J.V. J. Cell Biol. 141:967-977(1998) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, MASS SPECTROMETRY.
[6]	"The yeast spindle pole body component Spc72p interacts with Stu2p and is required for proper microtubule assembly." Chen X.P., Yin H., Huffaker T.C. J. Cell Biol. 141:1169-1179(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH STU2.
[7]	"SPC72: a spindle pole component required for spindle orientation in the yeast Saccharomyces cerevisiae." Soues S., Adams I.R. J. Cell Sci. 111:2809-2818(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"Interaction of the yeast gamma-tubulin complex-binding protein Spc72p with Kar1p is essential for microtubule function during karyogamy." Pereira G., Grueneberg U., Knop M., Schiebel E. EMBO J. 18:4180-4195(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH KAR1.
[9]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"A coupled chemical-genetic and bioinformatic approach to Polo-like kinase pathway exploration." Snead J.L., Sullivan M., Lowery D.M., Cohen M.S., Zhang C., Randle D.H., Taunton J., Yaffe M.B., Morgan D.O., Shokat K.M. Chem. Biol. 14:1261-1272(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CDC5, PHOSPHORYLATION BY CDC5.
[12]	"The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal." Maekawa H., Priest C., Lechner J., Pereira G., Schiebel E. J. Cell Biol. 179:423-436(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION BY CDC5, INTERACTION WITH KIN4.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12980 Genomic DNA. Translation: AAC04984.1. AY723757 Genomic DNA. Translation: AAU09674.1. BK006935 Genomic DNA. Translation: DAA06939.2.
PIR	S51972.
RefSeq	NP_009352.2. NM_001178192.2.
3D structure databases
ProteinModelPortal	P39723.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-868N.
IntAct	P39723. 36 interactions.
MINT	MINT-385032.
STRING	4932.YAL047C.
Proteomic databases
PaxDb	P39723.
PRIDE	P39723.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL047C; YAL047C; YAL047C.
GeneID	851250.
KEGG	sce:YAL047C.
Organism-specific databases
CYGD	YAL047c.
SGD	S000000045. SPC72.
Phylogenomic databases
eggNOG	NOG129411.
OrthoDB	EOG412QFN.
Gene expression databases
Genevestigator	P39723.
GermOnline	YAL047C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR024545. Mto2p-binding. [Graphical view]
Pfam	PF12808. Mto2_bdg. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968195.

Entry information

Entry name

SPC72_YEAST

Accession

Primary (citable) accession number: P39723
Secondary accession number(s): D6VPG9, E9P941

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program