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P39722 (GEM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial Rho GTPase 1

EC=3.6.5.-
Alternative name(s):
GTPase EF-hand protein of mitochondria 1
Gene names
Name:GEM1
Ordered Locus Names:YAL048C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length662 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution Probable. Ref.4 Ref.5

Subcellular location

Mitochondrion outer membrane; Single-pass type IV membrane protein Ref.3 Ref.5.

Disruption phenotype

Collapsed, globular, or grape-like mitochondria. Ref.5

Sequence similarities

Belongs to the mitochondrial Rho GTPase family.

Contains 2 EF-hand domains.

Contains 2 Miro domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 662662Mitochondrial Rho GTPase 1
PRO_0000202422

Regions

Topological domain1 – 634634Mitochondrial intermembrane Potential
Transmembrane635 – 65521Helical; Anchor for type IV membrane protein; Potential
Topological domain656 – 6627Cytoplasmic Potential
Domain1 – 140140Miro 1
Domain201 – 23636EF-hand 1
Domain330 – 36536EF-hand 2
Domain442 – 662221Miro 2
Nucleotide binding12 – 198GTP 1 Probable
Nucleotide binding62 – 643GTP 1 Potential
Nucleotide binding116 – 1194GTP 1 Potential
Calcium binding214 – 225121 Probable
Calcium binding343 – 354122 Probable
Nucleotide binding455 – 4628GTP 2 Probable
Nucleotide binding491 – 4955GTP 2 Potential
Nucleotide binding560 – 5634GTP 2 Potential

Experimental info

Mutagenesis181K → A: Loss of function. Ref.5
Mutagenesis191S → N: Loss of function. Ref.5
Mutagenesis331T → A: No effect. Ref.5
Mutagenesis2251E → K: Induces collapsed, globular or grape-like mitochondria; when associated with A-354. Ref.5
Mutagenesis3541E → K: Induces collapsed, globular or grape-like mitochondria; when associated with A-225. Ref.5
Mutagenesis4611K → A: Induces collapsed, globular or grape-like mitochondria. Ref.5
Mutagenesis4621S → N: Induces collapsed, globular or grape-like mitochondria. Ref.5
Mutagenesis4801T → A: Induces collapsed, globular or grape-like mitochondria. Ref.5

Sequences

Sequence LengthMass (Da)Tools
P39722 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: A68CDA15D79E5910

FASTA66275,150
        10         20         30         40         50         60 
MTKETIRVVI CGDEGVGKSS LIVSLTKAEF IPTIQDVLPP ISIPRDFSSS PTYSPKNTVL 

        70         80         90        100        110        120 
IDTSDSDLIA LDHELKSADV IWLVYCDHES YDHVSLFWLP HFRSLGLNIP VILCKNKCDS 

       130        140        150        160        170        180 
ISNVNANAMV VSENSDDDID TKVEDEEFIP ILMEFKEIDT CIKTSAKTQF DLNQAFYLCQ 

       190        200        210        220        230        240 
RAITHPISPL FDAMVGELKP LAVMALKRIF LLSDLNQDSY LDDNEILGLQ KKCFNKSIDV 

       250        260        270        280        290        300 
NELNFIKDLL LDISKHDQEY INRKLYVPGK GITKDGFLVL NKIYAERGRH ETTWAILRTF 

       310        320        330        340        350        360 
HYTDSLCIND KILHPRLVVP DTSSVELSPK GYRFLVDIFL KFDIDNDGGL NNQELHRLFK 

       370        380        390        400        410        420 
CTPGLPKLWT STNFPFSTVV NNKGCITLQG WLAQWSMTTF LNYSTTTAYL VYFGFQEDAR 

       430        440        450        460        470        480 
LALQVTKPRK MRRRSGKLYR SNINDRKVFN CFVIGKPCCG KSSLLEAFLG RSFSEEYSPT 

       490        500        510        520        530        540 
IKPRIAVNSL ELKGGKQYYL ILQELGEQEY AILENKDKLK ECDVICLTYD SSDPESFSYL 

       550        560        570        580        590        600 
VSLLDKFTHL QDLPLVFVAS KADLDKQQQR CQIQPDELAD ELFVNHPLHI SSRWLSSLNE 

       610        620        630        640        650        660 
LFIKITEAAL DPGKNTPGLP EETAAKDVDY RQTALIFGST VGFVALCSFT LMKLFKSSKF 


SK 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins."
Zahedi R.P., Sickmann A., Boehm A.M., Winkler C., Zufall N., Schoenfisch B., Guiard B., Pfanner N., Meisinger C.
Mol. Biol. Cell 17:1436-1450(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[4]"The open reading frame YAL048c affects the secretion of proteinase A in S. cerevisiae."
Wolff A.M., Petersen J.G., Nilsson-Tillgren T., Din N.
Yeast 15:427-434(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY FUNCTION.
[5]"Yeast Miro GTPase, Gem1p, regulates mitochondrial morphology via a novel pathway."
Frederick R.L., McCaffery J.M., Cunningham K.W., Okamoto K., Shaw J.M.
J. Cell Biol. 167:87-98(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF LYS-18; SER-19; THR-33; GLU-225; GLU-354; LYS-461; SER-462 AND THR-480, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12980 Genomic DNA. Translation: AAC04983.1.
BK006935 Genomic DNA. Translation: DAA06938.1.
PIRS51971.
RefSeqNP_009351.1. NM_001178193.1.

3D structure databases

ProteinModelPortalP39722.
SMRP39722. Positions 3-183, 197-612.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31779. 122 interactions.
DIPDIP-8142N.
IntActP39722. 16 interactions.
STRING4932.YAL048C.

Proteomic databases

PaxDbP39722.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAL048C; YAL048C; YAL048C.
GeneID851249.
KEGGsce:YAL048C.

Organism-specific databases

CYGDYAL048c.
SGDS000000046. GEM1.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00390000014374.
HOGENOMHOG000215553.
KOK07870.
OMAQDYLFPP.
OrthoDBEOG73545F.

Enzyme and pathway databases

BioCycYEAST:G3O-28855-MONOMER.

Gene expression databases

GenevestigatorP39722.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR013566. EF_hand_assoc_1.
IPR013567. EF_hand_assoc_2.
IPR002048. EF_hand_dom.
IPR020860. MIRO.
IPR013684. MIRO-like.
IPR027417. P-loop_NTPase.
IPR021181. Rho_GTPase_Mt.
IPR001806. Small_GTPase.
[Graphical view]
PANTHERPTHR24072:SF69. PTHR24072:SF69. 1 hit.
PfamPF08355. EF_assoc_1. 1 hit.
PF08356. EF_assoc_2. 1 hit.
PF08477. Miro. 2 hits.
[Graphical view]
PIRSFPIRSF037488. Mt_Rho_GTPase. 1 hit.
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00054. EFh. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS50222. EF_HAND_2. 2 hits.
PS51423. MIRO. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio968192.

Entry information

Entry nameGEM1_YEAST
AccessionPrimary (citable) accession number: P39722
Secondary accession number(s): D6VPG8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families