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Protein

Mitochondrial Rho GTPase 1

Gene

GEM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution (Probable).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 19GTP 1Curated8
Nucleotide bindingi62 – 64GTP 1Sequence analysis3
Nucleotide bindingi116 – 119GTP 1Sequence analysis4
Calcium bindingi214 – 2251CuratedAdd BLAST12
Calcium bindingi343 – 3542CuratedAdd BLAST12
Nucleotide bindingi455 – 462GTP 2Curated8
Nucleotide bindingi491 – 495GTP 2Sequence analysis5
Nucleotide bindingi560 – 563GTP 2Sequence analysis4

GO - Molecular functioni

  • calcium ion binding Source: SGD
  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • mitochondrion-ER tethering Source: SGD
  • mitochondrion inheritance Source: SGD
  • phospholipid homeostasis Source: SGD
  • regulation of mitochondrion organization Source: SGD
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, GTP-binding, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28855-MONOMER.
ReactomeiR-SCE-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial Rho GTPase 1 (EC:3.6.5.-)
Alternative name(s):
GTPase EF-hand protein of mitochondria 1
Gene namesi
Name:GEM1
Ordered Locus Names:YAL048C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL048C.
SGDiS000000046. GEM1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 634Mitochondrial intermembraneSequence analysisAdd BLAST634
Transmembranei635 – 655Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini656 – 662CytoplasmicSequence analysis7

GO - Cellular componenti

  • ERMES complex Source: SGD
  • integral component of mitochondrial outer membrane Source: InterPro
  • mitochondrial outer membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Disruption phenotypei

Collapsed, globular, or grape-like mitochondria.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi18K → A: Loss of function. 1 Publication1
Mutagenesisi19S → N: Loss of function. 1 Publication1
Mutagenesisi33T → A: No effect. 1 Publication1
Mutagenesisi225E → K: Induces collapsed, globular or grape-like mitochondria; when associated with A-354. 1 Publication1
Mutagenesisi354E → K: Induces collapsed, globular or grape-like mitochondria; when associated with A-225. 1 Publication1
Mutagenesisi461K → A: Induces collapsed, globular or grape-like mitochondria. 1 Publication1
Mutagenesisi462S → N: Induces collapsed, globular or grape-like mitochondria. 1 Publication1
Mutagenesisi480T → A: Induces collapsed, globular or grape-like mitochondria. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002024221 – 662Mitochondrial Rho GTPase 1Add BLAST662

Proteomic databases

MaxQBiP39722.
PRIDEiP39722.

Interactioni

Protein-protein interaction databases

BioGridi31779. 123 interactors.
DIPiDIP-8142N.
IntActiP39722. 16 interactors.

Structurei

3D structure databases

ProteinModelPortaliP39722.
SMRiP39722.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 140Miro 1Add BLAST140
Domaini201 – 236EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini330 – 365EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini442 – 662Miro 2Add BLAST221

Sequence similaritiesi

Belongs to the mitochondrial Rho GTPase family.Curated
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 2 Miro domains.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000014374.
HOGENOMiHOG000215553.
InParanoidiP39722.
KOiK07870.
OMAiCFDKPLS.
OrthoDBiEOG092C13Q5.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR013566. EF_hand_assoc_1.
IPR013567. EF_hand_assoc_2.
IPR002048. EF_hand_dom.
IPR020860. MIRO_dom.
IPR021181. Mt_Rho_GTPase.
IPR027417. P-loop_NTPase.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF08355. EF_assoc_1. 1 hit.
PF08356. EF_assoc_2. 1 hit.
PF00071. Ras. 2 hits.
[Graphical view]
PIRSFiPIRSF037488. Mt_Rho_GTPase. 1 hit.
SUPFAMiSSF47473. SSF47473. 2 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS50222. EF_HAND_2. 2 hits.
PS51423. MIRO. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKETIRVVI CGDEGVGKSS LIVSLTKAEF IPTIQDVLPP ISIPRDFSSS
60 70 80 90 100
PTYSPKNTVL IDTSDSDLIA LDHELKSADV IWLVYCDHES YDHVSLFWLP
110 120 130 140 150
HFRSLGLNIP VILCKNKCDS ISNVNANAMV VSENSDDDID TKVEDEEFIP
160 170 180 190 200
ILMEFKEIDT CIKTSAKTQF DLNQAFYLCQ RAITHPISPL FDAMVGELKP
210 220 230 240 250
LAVMALKRIF LLSDLNQDSY LDDNEILGLQ KKCFNKSIDV NELNFIKDLL
260 270 280 290 300
LDISKHDQEY INRKLYVPGK GITKDGFLVL NKIYAERGRH ETTWAILRTF
310 320 330 340 350
HYTDSLCIND KILHPRLVVP DTSSVELSPK GYRFLVDIFL KFDIDNDGGL
360 370 380 390 400
NNQELHRLFK CTPGLPKLWT STNFPFSTVV NNKGCITLQG WLAQWSMTTF
410 420 430 440 450
LNYSTTTAYL VYFGFQEDAR LALQVTKPRK MRRRSGKLYR SNINDRKVFN
460 470 480 490 500
CFVIGKPCCG KSSLLEAFLG RSFSEEYSPT IKPRIAVNSL ELKGGKQYYL
510 520 530 540 550
ILQELGEQEY AILENKDKLK ECDVICLTYD SSDPESFSYL VSLLDKFTHL
560 570 580 590 600
QDLPLVFVAS KADLDKQQQR CQIQPDELAD ELFVNHPLHI SSRWLSSLNE
610 620 630 640 650
LFIKITEAAL DPGKNTPGLP EETAAKDVDY RQTALIFGST VGFVALCSFT
660
LMKLFKSSKF SK
Length:662
Mass (Da):75,150
Last modified:February 1, 1995 - v1
Checksum:iA68CDA15D79E5910
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. Translation: AAC04983.1.
BK006935 Genomic DNA. Translation: DAA06938.1.
PIRiS51971.
RefSeqiNP_009351.1. NM_001178193.1.

Genome annotation databases

EnsemblFungiiYAL048C; YAL048C; YAL048C.
GeneIDi851249.
KEGGisce:YAL048C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. Translation: AAC04983.1.
BK006935 Genomic DNA. Translation: DAA06938.1.
PIRiS51971.
RefSeqiNP_009351.1. NM_001178193.1.

3D structure databases

ProteinModelPortaliP39722.
SMRiP39722.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31779. 123 interactors.
DIPiDIP-8142N.
IntActiP39722. 16 interactors.

Proteomic databases

MaxQBiP39722.
PRIDEiP39722.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL048C; YAL048C; YAL048C.
GeneIDi851249.
KEGGisce:YAL048C.

Organism-specific databases

EuPathDBiFungiDB:YAL048C.
SGDiS000000046. GEM1.

Phylogenomic databases

GeneTreeiENSGT00390000014374.
HOGENOMiHOG000215553.
InParanoidiP39722.
KOiK07870.
OMAiCFDKPLS.
OrthoDBiEOG092C13Q5.

Enzyme and pathway databases

BioCyciYEAST:G3O-28855-MONOMER.
ReactomeiR-SCE-194840. Rho GTPase cycle.

Miscellaneous databases

PROiP39722.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
3.40.50.300. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR013566. EF_hand_assoc_1.
IPR013567. EF_hand_assoc_2.
IPR002048. EF_hand_dom.
IPR020860. MIRO_dom.
IPR021181. Mt_Rho_GTPase.
IPR027417. P-loop_NTPase.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF08355. EF_assoc_1. 1 hit.
PF08356. EF_assoc_2. 1 hit.
PF00071. Ras. 2 hits.
[Graphical view]
PIRSFiPIRSF037488. Mt_Rho_GTPase. 1 hit.
SUPFAMiSSF47473. SSF47473. 2 hits.
SSF52540. SSF52540. 2 hits.
PROSITEiPS50222. EF_HAND_2. 2 hits.
PS51423. MIRO. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGEM1_YEAST
AccessioniPrimary (citable) accession number: P39722
Secondary accession number(s): D6VPG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.