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P39722

- GEM1_YEAST

UniProt

P39722 - GEM1_YEAST

Protein

Mitochondrial Rho GTPase 1

Gene

GEM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution Probable.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 198GTP 1Curated
    Nucleotide bindingi62 – 643GTP 1Sequence Analysis
    Nucleotide bindingi116 – 1194GTP 1Sequence Analysis
    Calcium bindingi214 – 225121CuratedAdd
    BLAST
    Calcium bindingi343 – 354122CuratedAdd
    BLAST
    Nucleotide bindingi455 – 4628GTP 2Curated
    Nucleotide bindingi491 – 4955GTP 2Sequence Analysis
    Nucleotide bindingi560 – 5634GTP 2Sequence Analysis

    GO - Molecular functioni

    1. calcium ion binding Source: SGD
    2. GTPase activity Source: SGD
    3. GTP binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular homeostasis Source: InterPro
    2. GTP catabolic process Source: GOC
    3. mitochondrion inheritance Source: SGD
    4. phospholipid homeostasis Source: SGD
    5. regulation of mitochondrion organization Source: SGD
    6. small GTPase mediated signal transduction Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Calcium, GTP-binding, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28855-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial Rho GTPase 1 (EC:3.6.5.-)
    Alternative name(s):
    GTPase EF-hand protein of mitochondria 1
    Gene namesi
    Name:GEM1
    Ordered Locus Names:YAL048C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAL048c.
    SGDiS000000046. GEM1.

    Subcellular locationi

    Mitochondrion outer membrane 2 Publications; Single-pass type IV membrane protein 2 Publications

    GO - Cellular componenti

    1. ERMES complex Source: SGD
    2. integral component of membrane Source: UniProtKB-KW
    3. mitochondrial outer membrane Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    Pathology & Biotechi

    Disruption phenotypei

    Collapsed, globular, or grape-like mitochondria.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi18 – 181K → A: Loss of function. 1 Publication
    Mutagenesisi19 – 191S → N: Loss of function. 1 Publication
    Mutagenesisi33 – 331T → A: No effect. 1 Publication
    Mutagenesisi225 – 2251E → K: Induces collapsed, globular or grape-like mitochondria; when associated with A-354. 1 Publication
    Mutagenesisi354 – 3541E → K: Induces collapsed, globular or grape-like mitochondria; when associated with A-225. 1 Publication
    Mutagenesisi461 – 4611K → A: Induces collapsed, globular or grape-like mitochondria. 1 Publication
    Mutagenesisi462 – 4621S → N: Induces collapsed, globular or grape-like mitochondria. 1 Publication
    Mutagenesisi480 – 4801T → A: Induces collapsed, globular or grape-like mitochondria. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 662662Mitochondrial Rho GTPase 1PRO_0000202422Add
    BLAST

    Proteomic databases

    MaxQBiP39722.
    PaxDbiP39722.

    Expressioni

    Gene expression databases

    GenevestigatoriP39722.

    Interactioni

    Protein-protein interaction databases

    BioGridi31779. 122 interactions.
    DIPiDIP-8142N.
    IntActiP39722. 16 interactions.
    STRINGi4932.YAL048C.

    Structurei

    3D structure databases

    ProteinModelPortaliP39722.
    SMRiP39722. Positions 3-183, 197-612.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 634634Mitochondrial intermembraneSequence AnalysisAdd
    BLAST
    Topological domaini656 – 6627CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei635 – 65521Helical; Anchor for type IV membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 140140Miro 1Add
    BLAST
    Domaini201 – 23636EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini330 – 36536EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini442 – 662221Miro 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the mitochondrial Rho GTPase family.Curated
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 2 Miro domains.Curated

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00390000014374.
    HOGENOMiHOG000215553.
    KOiK07870.
    OMAiNRCTFCL.
    OrthoDBiEOG73545F.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR013566. EF_hand_assoc_1.
    IPR013567. EF_hand_assoc_2.
    IPR002048. EF_hand_dom.
    IPR020860. MIRO.
    IPR013684. MIRO-like.
    IPR027417. P-loop_NTPase.
    IPR021181. Rho_GTPase_Mt.
    IPR001806. Small_GTPase.
    [Graphical view]
    PfamiPF08355. EF_assoc_1. 1 hit.
    PF08356. EF_assoc_2. 1 hit.
    PF08477. Miro. 2 hits.
    [Graphical view]
    PIRSFiPIRSF037488. Mt_Rho_GTPase. 1 hit.
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS50222. EF_HAND_2. 2 hits.
    PS51423. MIRO. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39722-1 [UniParc]FASTAAdd to Basket

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    MTKETIRVVI CGDEGVGKSS LIVSLTKAEF IPTIQDVLPP ISIPRDFSSS    50
    PTYSPKNTVL IDTSDSDLIA LDHELKSADV IWLVYCDHES YDHVSLFWLP 100
    HFRSLGLNIP VILCKNKCDS ISNVNANAMV VSENSDDDID TKVEDEEFIP 150
    ILMEFKEIDT CIKTSAKTQF DLNQAFYLCQ RAITHPISPL FDAMVGELKP 200
    LAVMALKRIF LLSDLNQDSY LDDNEILGLQ KKCFNKSIDV NELNFIKDLL 250
    LDISKHDQEY INRKLYVPGK GITKDGFLVL NKIYAERGRH ETTWAILRTF 300
    HYTDSLCIND KILHPRLVVP DTSSVELSPK GYRFLVDIFL KFDIDNDGGL 350
    NNQELHRLFK CTPGLPKLWT STNFPFSTVV NNKGCITLQG WLAQWSMTTF 400
    LNYSTTTAYL VYFGFQEDAR LALQVTKPRK MRRRSGKLYR SNINDRKVFN 450
    CFVIGKPCCG KSSLLEAFLG RSFSEEYSPT IKPRIAVNSL ELKGGKQYYL 500
    ILQELGEQEY AILENKDKLK ECDVICLTYD SSDPESFSYL VSLLDKFTHL 550
    QDLPLVFVAS KADLDKQQQR CQIQPDELAD ELFVNHPLHI SSRWLSSLNE 600
    LFIKITEAAL DPGKNTPGLP EETAAKDVDY RQTALIFGST VGFVALCSFT 650
    LMKLFKSSKF SK 662
    Length:662
    Mass (Da):75,150
    Last modified:February 1, 1995 - v1
    Checksum:iA68CDA15D79E5910
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12980 Genomic DNA. Translation: AAC04983.1.
    BK006935 Genomic DNA. Translation: DAA06938.1.
    PIRiS51971.
    RefSeqiNP_009351.1. NM_001178193.1.

    Genome annotation databases

    EnsemblFungiiYAL048C; YAL048C; YAL048C.
    GeneIDi851249.
    KEGGisce:YAL048C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12980 Genomic DNA. Translation: AAC04983.1 .
    BK006935 Genomic DNA. Translation: DAA06938.1 .
    PIRi S51971.
    RefSeqi NP_009351.1. NM_001178193.1.

    3D structure databases

    ProteinModelPortali P39722.
    SMRi P39722. Positions 3-183, 197-612.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31779. 122 interactions.
    DIPi DIP-8142N.
    IntActi P39722. 16 interactions.
    STRINGi 4932.YAL048C.

    Proteomic databases

    MaxQBi P39722.
    PaxDbi P39722.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL048C ; YAL048C ; YAL048C .
    GeneIDi 851249.
    KEGGi sce:YAL048C.

    Organism-specific databases

    CYGDi YAL048c.
    SGDi S000000046. GEM1.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00390000014374.
    HOGENOMi HOG000215553.
    KOi K07870.
    OMAi NRCTFCL.
    OrthoDBi EOG73545F.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28855-MONOMER.

    Miscellaneous databases

    NextBioi 968192.

    Gene expression databases

    Genevestigatori P39722.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR013566. EF_hand_assoc_1.
    IPR013567. EF_hand_assoc_2.
    IPR002048. EF_hand_dom.
    IPR020860. MIRO.
    IPR013684. MIRO-like.
    IPR027417. P-loop_NTPase.
    IPR021181. Rho_GTPase_Mt.
    IPR001806. Small_GTPase.
    [Graphical view ]
    Pfami PF08355. EF_assoc_1. 1 hit.
    PF08356. EF_assoc_2. 1 hit.
    PF08477. Miro. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF037488. Mt_Rho_GTPase. 1 hit.
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS50222. EF_HAND_2. 2 hits.
    PS51423. MIRO. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins."
      Zahedi R.P., Sickmann A., Boehm A.M., Winkler C., Zufall N., Schoenfisch B., Guiard B., Pfanner N., Meisinger C.
      Mol. Biol. Cell 17:1436-1450(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
    4. "The open reading frame YAL048c affects the secretion of proteinase A in S. cerevisiae."
      Wolff A.M., Petersen J.G., Nilsson-Tillgren T., Din N.
      Yeast 15:427-434(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY FUNCTION.
    5. "Yeast Miro GTPase, Gem1p, regulates mitochondrial morphology via a novel pathway."
      Frederick R.L., McCaffery J.M., Cunningham K.W., Okamoto K., Shaw J.M.
      J. Cell Biol. 167:87-98(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF LYS-18; SER-19; THR-33; GLU-225; GLU-354; LYS-461; SER-462 AND THR-480, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiGEM1_YEAST
    AccessioniPrimary (citable) accession number: P39722
    Secondary accession number(s): D6VPG8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3