Mitochondrial Rho GTPase 1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P39722 (GEM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Mitochondrial Rho GTPase 1
EC=3.6.5.-

Alternative name(s):
GTPase EF-hand protein of mitochondria 1

Gene names

Name:	GEM1
Ordered Locus Names:	YAL048C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	662 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitochondrial GTPase involved in mitochondrial trafficking. Probably involved in control of anterograde transport of mitochondria and their subcellular distribution Probable. Ref.4 Ref.5
Subcellular location	Mitochondrion outer membrane; Single-pass type IV membrane protein Ref.3 Ref.5.
Disruption phenotype	Collapsed, globular, or grape-like mitochondria. Ref.5
Sequence similarities	Belongs to the mitochondrial Rho GTPase family. Contains 2 EF-hand domains. Contains 2 Miro domains.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Repeat Transmembrane Transmembrane helix
Ligand	Calcium GTP-binding Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular homeostasis Inferred from electronic annotation. Source: InterPro mitochondrion inheritance Inferred from mutant phenotype PubMed 18245340. Source: SGD phospholipid homeostasis Inferred from genetic interaction PubMed 21825164. Source: SGD regulation of mitochondrion organization Inferred from mutant phenotype PubMed 21825164. Source: SGD small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	ERMES complex Inferred from physical interaction PubMed 21825164 PubMed 21945531. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from direct assay Ref.5. Source: SGD calcium ion binding Inferred from direct assay PubMed 21036903. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 662

662

Mitochondrial Rho GTPase 1

PRO_0000202422

Regions

Topological domain

1 – 634

634

Mitochondrial intermembrane Potential

Transmembrane

635 – 655

Helical; Anchor for type IV membrane protein; Potential

Topological domain

656 – 662

Cytoplasmic Potential

Domain

1 – 140

140

Miro 1

Domain

201 – 236

EF-hand 1

Domain

330 – 365

EF-hand 2

Domain

442 – 662

221

Miro 2

Nucleotide binding

12 – 19

GTP 1 Probable

Nucleotide binding

62 – 64

GTP 1 Potential

Nucleotide binding

116 – 119

GTP 1 Potential

Calcium binding

214 – 225

1 Probable

Calcium binding

343 – 354

2 Probable

Nucleotide binding

455 – 462

GTP 2 Probable

Nucleotide binding

491 – 495

GTP 2 Potential

Nucleotide binding

560 – 563

GTP 2 Potential

Experimental info

Mutagenesis

K → A: Loss of function. Ref.5

Mutagenesis

S → N: Loss of function. Ref.5

Mutagenesis

T → A: No effect. Ref.5

Mutagenesis

225

E → K: Induces collapsed, globular or grape-like mitochondria; when associated with A-354. Ref.5

Mutagenesis

354

E → K: Induces collapsed, globular or grape-like mitochondria; when associated with A-225. Ref.5

Mutagenesis

461

K → A: Induces collapsed, globular or grape-like mitochondria. Ref.5

Mutagenesis

462

S → N: Induces collapsed, globular or grape-like mitochondria. Ref.5

Mutagenesis

480

T → A: Induces collapsed, globular or grape-like mitochondria. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P39722 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: A68CDA15D79E5910
Show »

FASTA

662

75,150

        10         20         30         40         50         60 
MTKETIRVVI CGDEGVGKSS LIVSLTKAEF IPTIQDVLPP ISIPRDFSSS PTYSPKNTVL 

        70         80         90        100        110        120 
IDTSDSDLIA LDHELKSADV IWLVYCDHES YDHVSLFWLP HFRSLGLNIP VILCKNKCDS 

       130        140        150        160        170        180 
ISNVNANAMV VSENSDDDID TKVEDEEFIP ILMEFKEIDT CIKTSAKTQF DLNQAFYLCQ 

       190        200        210        220        230        240 
RAITHPISPL FDAMVGELKP LAVMALKRIF LLSDLNQDSY LDDNEILGLQ KKCFNKSIDV 

       250        260        270        280        290        300 
NELNFIKDLL LDISKHDQEY INRKLYVPGK GITKDGFLVL NKIYAERGRH ETTWAILRTF 

       310        320        330        340        350        360 
HYTDSLCIND KILHPRLVVP DTSSVELSPK GYRFLVDIFL KFDIDNDGGL NNQELHRLFK 

       370        380        390        400        410        420 
CTPGLPKLWT STNFPFSTVV NNKGCITLQG WLAQWSMTTF LNYSTTTAYL VYFGFQEDAR 

       430        440        450        460        470        480 
LALQVTKPRK MRRRSGKLYR SNINDRKVFN CFVIGKPCCG KSSLLEAFLG RSFSEEYSPT 

       490        500        510        520        530        540 
IKPRIAVNSL ELKGGKQYYL ILQELGEQEY AILENKDKLK ECDVICLTYD SSDPESFSYL 

       550        560        570        580        590        600 
VSLLDKFTHL QDLPLVFVAS KADLDKQQQR CQIQPDELAD ELFVNHPLHI SSRWLSSLNE 

       610        620        630        640        650        660 
LFIKITEAAL DPGKNTPGLP EETAAKDVDY RQTALIFGST VGFVALCSFT LMKLFKSSKF 


SK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins." Zahedi R.P., Sickmann A., Boehm A.M., Winkler C., Zufall N., Schoenfisch B., Guiard B., Pfanner N., Meisinger C. Mol. Biol. Cell 17:1436-1450(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.
[4]	"The open reading frame YAL048c affects the secretion of proteinase A in S. cerevisiae." Wolff A.M., Petersen J.G., Nilsson-Tillgren T., Din N. Yeast 15:427-434(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PRELIMINARY FUNCTION.
[5]	"Yeast Miro GTPase, Gem1p, regulates mitochondrial morphology via a novel pathway." Frederick R.L., McCaffery J.M., Cunningham K.W., Okamoto K., Shaw J.M. J. Cell Biol. 167:87-98(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, MUTAGENESIS OF LYS-18; SER-19; THR-33; GLU-225; GLU-354; LYS-461; SER-462 AND THR-480, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12980 Genomic DNA. Translation: AAC04983.1. BK006935 Genomic DNA. Translation: DAA06938.1.
PIR	S51971.
RefSeq	NP_009351.1. NM_001178193.1.
3D structure databases
ProteinModelPortal	P39722.
SMR	P39722. Positions 3-183, 205-230, 335-394, 448-609.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-8142N.
IntAct	P39722. 16 interactions.
STRING	4932.YAL048C.
Proteomic databases
PaxDb	P39722.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL048C; YAL048C; YAL048C.
GeneID	851249.
KEGG	sce:YAL048C.
Organism-specific databases
CYGD	YAL048c.
SGD	S000000046. GEM1.
Phylogenomic databases
eggNOG	COG1100.
GeneTree	ENSGT00390000014374.
HOGENOM	HOG000215553.
KO	K07870.
OMA	LELNQDY.
OrthoDB	EOG4NCQMX.
Gene expression databases
Genevestigator	P39722.
GermOnline	YAL048C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013566. EF_hand_assoc_1. IPR013567. EF_hand_assoc_2. IPR002048. EF_hand_dom. IPR020860. MIRO. IPR013684. MIRO-like. IPR001806. Small_GTPase. IPR021181. Small_GTPase_Miro. [Graphical view]
PANTHER	PTHR24072:SF69. PTHR24072:SF69. 1 hit.
Pfam	PF08355. EF_assoc_1. 1 hit. PF08356. EF_assoc_2. 1 hit. PF08477. Miro. 2 hits. [Graphical view]
PIRSF	PIRSF037488. Mt_Rho_GTPase. 1 hit.
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. False negative. PS50222. EF_HAND_2. 2 hits. PS51423. MIRO. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	968192.

Entry information

Entry name

GEM1_YEAST

Accession

Primary (citable) accession number: P39722
Secondary accession number(s): D6VPG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents