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Protein

Oleate-activated transcription factor 1

Gene

OAF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The PIP2-OAF1 heterodimer acts as a transcriptional activator to induce the transcription of genes encoding proteins involved in fatty acid beta-oxidation, a response called oleic acid induction, when cells grow on fatty acids as sole carbon source. Recognizes and binds to the oleate response element (ORE) (or peroxisome box), two inverted CGG triplets spaced by 14 to 18 intervening nucleotides, in the promoter region of a number of genes (such as CTA1, FOX1 to FOX3, FAA2, PAS8, PAS10, etc.) for peroxisomal proteins. OAF1 acts as the sensor for oleate and inhibits PIP2 activity under non-inducing conditions. Activity is repressed by glucose.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi66 – 93Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

GO - Biological processi

  • positive regulation of chromatin silencing at telomere Source: SGD
  • positive regulation of fatty acid beta-oxidation Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter by oleic acid Source: SGD
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Sporulation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-28857-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Oleate-activated transcription factor 1
Gene namesi
Name:OAF1
Synonyms:YAF1
Ordered Locus Names:YAL051W
ORF Names:FUN43
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL051W.
SGDiS000000048. OAF1.

Subcellular locationi

GO - Cellular componenti

  • Pip2-Oaf1 complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001149901 – 1047Oleate-activated transcription factor 1Add BLAST1047

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei155PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39720.
PRIDEiP39720.

PTM databases

iPTMnetiP39720.

Interactioni

Subunit structurei

Heterodimer of PIP2 and OAF1.

Protein-protein interaction databases

BioGridi31777. 48 interactors.
DIPiDIP-5928N.
IntActiP39720. 4 interactors.
MINTiMINT-2781292.

Structurei

3D structure databases

ProteinModelPortaliP39720.
SMRiP39720.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1034 – 10429aaTAD9

Domaini

the 9aaTAD motif (residues 1034 to 1042) is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000000033.
HOGENOMiHOG000066010.
InParanoidiP39720.
OMAiNRISFVC.
OrthoDBiEOG092C1Q2S.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39720-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVENSTQKAP HAGNDDNSST KPYSEAFFLG FNNPTPGLEA EHSSTSPAPE
60 70 80 90 100
NSETHNRKRN RILFVCQACR KSKTKCDREK PECGRCVKHG LKCVYDVSKQ
110 120 130 140 150
PAPRIPSKDA IISRLEKDMF YWKDKAMKLL TEREVNESGK RSASPINTNN
160 170 180 190 200
ASGDSPDTKK QHKMEPIYEQ SGNGDINNGT RNDIEINLYR SHPTMIMSKV
210 220 230 240 250
MKREVKPLSE NYIIIQDCFL KILVTSVFLD TSKNTMIPAL TANANITRAQ
260 270 280 290 300
PSVANNLLKL KEMLIRQCQT EDEKNRVNEF TDRILQNTNS NRNLKIGMLL
310 320 330 340 350
SMLYNSVGYQ YLEDHCPQGG EYSDLLRNLI NECEAILPSY EIIERYKNHF
360 370 380 390 400
YEYVYPSLPF IELEIFEESL SQTIFPDPNN PSKVQIRMGS THLRAKVENL
410 420 430 440 450
SLLLVILKLS YMSIRFLDHS TADSSFYLSK EIIDKYPIPN DFILLSQRCL
460 470 480 490 500
ASENWCACAN ENIISCLLYI WSFFAFSPEE GDFFLEHPTD VISSLIMMLS
510 520 530 540 550
TSIGLHRDPS DFPQLISPST SDKRTLNHRR ILWLSIVTVC SFEASLKGRH
560 570 580 590 600
SVSPISLMAL FLNIKDPDSL TVYMNRVRGD LSDINNHKLL RIHKFTFKRA
610 620 630 640 650
QLALLLSDLD NLTMTYYGSF HLHSIEFIRE KIEIFVEENF PIVPLKSVAQ
660 670 680 690 700
DKSDLDDMNV ISEMNILSSE NSSSFHNRIM NKLLMLRTSM AVFLHFETLI
710 720 730 740 750
TKDKSIFPFY KKYFMVSCMD ALSLINYFNK FFNGEYRHAI SSLTSFNVTK
760 770 780 790 800
FIQLALSSTI FSLLGIILRI GLAIHMLSSE VQKLSGTTDP RIKELNTKVE
810 820 830 840 850
KFSTLQRDLE SALEGIYCSA SEHLRFTYFP VFKMLALFDV IVQRMRKGEL
860 870 880 890 900
WHGIFTMIQM EQMHSRIIKT LSITLGVKLD KKDRLLEELM ACNHVANFSV
910 920 930 940 950
EDIDELNRNI KKEIQISSGL KPPVNTIDLT NGEPFGNAVP TFTKTWSSSL
960 970 980 990 1000
DNLEKLSSAA AVGQSLDYNS GLRQGPLAGG GSKEQTPIAG MNNLNNSINA
1010 1020 1030 1040
TPIVDNSSGS QLPNGFDRGQ ANNTPFPGYF GGLDLFDYDF LFGNDFA
Length:1,047
Mass (Da):118,891
Last modified:July 27, 2011 - v3
Checksum:i7D9388450486F540
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti70R → W in AAC04981 (PubMed:7731988).Curated1
Sequence conflicti447Q → P in AAC04981 (PubMed:7731988).Curated1
Sequence conflicti588K → T in AAC04981 (PubMed:7731988).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. Translation: AAC04981.2.
BK006935 Genomic DNA. Translation: DAA06936.2.
PIRiS51969.
RefSeqiNP_009349.3. NM_001178195.2.

Genome annotation databases

EnsemblFungiiYAL051W; YAL051W; YAL051W.
GeneIDi851247.
KEGGisce:YAL051W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. Translation: AAC04981.2.
BK006935 Genomic DNA. Translation: DAA06936.2.
PIRiS51969.
RefSeqiNP_009349.3. NM_001178195.2.

3D structure databases

ProteinModelPortaliP39720.
SMRiP39720.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31777. 48 interactors.
DIPiDIP-5928N.
IntActiP39720. 4 interactors.
MINTiMINT-2781292.

PTM databases

iPTMnetiP39720.

Proteomic databases

MaxQBiP39720.
PRIDEiP39720.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL051W; YAL051W; YAL051W.
GeneIDi851247.
KEGGisce:YAL051W.

Organism-specific databases

EuPathDBiFungiDB:YAL051W.
SGDiS000000048. OAF1.

Phylogenomic databases

GeneTreeiENSGT00390000000033.
HOGENOMiHOG000066010.
InParanoidiP39720.
OMAiNRISFVC.
OrthoDBiEOG092C1Q2S.

Enzyme and pathway databases

BioCyciYEAST:G3O-28857-MONOMER.

Miscellaneous databases

PROiP39720.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF00172. Zn_clus. 1 hit.
[Graphical view]
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOAF1_YEAST
AccessioniPrimary (citable) accession number: P39720
Secondary accession number(s): D6VPG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 92 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.