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P39720

- OAF1_YEAST

UniProt

P39720 - OAF1_YEAST

Protein

Oleate-activated transcription factor 1

Gene

OAF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    The PIP2-OAF1 heterodimer acts as a transcriptional activator to induce the transcription of genes encoding proteins involved in fatty acid beta-oxidation, a response called oleic acid induction, when cells grow on fatty acids as sole carbon source. Recognizes and binds to the oleate response element (ORE) (or peroxisome box), two inverted CGG triplets spaced by 14 to 18 intervening nucleotides, in the promoter region of a number of genes (such as CTA1, FOX1 to FOX3, FAA2, PAS8, PAS10, etc.) for peroxisomal proteins. OAF1 acts as the sensor for oleate and inhibits PIP2 activity under non-inducing conditions. Activity is repressed by glucose.4 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi66 – 9328Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
    2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cellular response to oleic acid Source: SGD
    2. positive regulation of chromatin silencing at telomere Source: SGD
    3. positive regulation of fatty acid beta-oxidation Source: SGD
    4. positive regulation of transcription from RNA polymerase II promoter Source: SGD
    5. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW

    Keywords - Biological processi

    Sporulation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28857-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Oleate-activated transcription factor 1
    Gene namesi
    Name:OAF1
    Synonyms:YAF1
    Ordered Locus Names:YAL051W
    ORF Names:FUN43
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAL051w.
    SGDiS000000048. OAF1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10471047Oleate-activated transcription factor 1PRO_0000114990Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei155 – 1551Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39720.
    PaxDbiP39720.
    PeptideAtlasiP39720.

    Expressioni

    Gene expression databases

    GenevestigatoriP39720.

    Interactioni

    Subunit structurei

    Heterodimer of PIP2 and OAF1.

    Protein-protein interaction databases

    BioGridi31777. 46 interactions.
    DIPiDIP-5928N.
    IntActiP39720. 4 interactions.
    MINTiMINT-2781292.
    STRINGi4932.YAL051W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39720.
    SMRiP39720. Positions 57-133.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1034 – 104299aaTAD

    Domaini

    the 9aaTAD motif (residues 1034 to 1042) is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

    Sequence similaritiesi

    Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG39949.
    GeneTreeiENSGT00390000000033.
    HOGENOMiHOG000066010.
    OMAiTKCDREK.
    OrthoDBiEOG7HF1SV.

    Family and domain databases

    Gene3Di4.10.240.10. 1 hit.
    InterProiIPR002409. Aflatoxin_BRP.
    IPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view]
    PfamiPF00172. Zn_clus. 1 hit.
    [Graphical view]
    PRINTSiPR00755. AFLATOXINBRP.
    SMARTiSM00066. GAL4. 1 hit.
    [Graphical view]
    SUPFAMiSSF57701. SSF57701. 1 hit.
    PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39720-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVENSTQKAP HAGNDDNSST KPYSEAFFLG FNNPTPGLEA EHSSTSPAPE     50
    NSETHNRKRN RILFVCQACR KSKTKCDREK PECGRCVKHG LKCVYDVSKQ 100
    PAPRIPSKDA IISRLEKDMF YWKDKAMKLL TEREVNESGK RSASPINTNN 150
    ASGDSPDTKK QHKMEPIYEQ SGNGDINNGT RNDIEINLYR SHPTMIMSKV 200
    MKREVKPLSE NYIIIQDCFL KILVTSVFLD TSKNTMIPAL TANANITRAQ 250
    PSVANNLLKL KEMLIRQCQT EDEKNRVNEF TDRILQNTNS NRNLKIGMLL 300
    SMLYNSVGYQ YLEDHCPQGG EYSDLLRNLI NECEAILPSY EIIERYKNHF 350
    YEYVYPSLPF IELEIFEESL SQTIFPDPNN PSKVQIRMGS THLRAKVENL 400
    SLLLVILKLS YMSIRFLDHS TADSSFYLSK EIIDKYPIPN DFILLSQRCL 450
    ASENWCACAN ENIISCLLYI WSFFAFSPEE GDFFLEHPTD VISSLIMMLS 500
    TSIGLHRDPS DFPQLISPST SDKRTLNHRR ILWLSIVTVC SFEASLKGRH 550
    SVSPISLMAL FLNIKDPDSL TVYMNRVRGD LSDINNHKLL RIHKFTFKRA 600
    QLALLLSDLD NLTMTYYGSF HLHSIEFIRE KIEIFVEENF PIVPLKSVAQ 650
    DKSDLDDMNV ISEMNILSSE NSSSFHNRIM NKLLMLRTSM AVFLHFETLI 700
    TKDKSIFPFY KKYFMVSCMD ALSLINYFNK FFNGEYRHAI SSLTSFNVTK 750
    FIQLALSSTI FSLLGIILRI GLAIHMLSSE VQKLSGTTDP RIKELNTKVE 800
    KFSTLQRDLE SALEGIYCSA SEHLRFTYFP VFKMLALFDV IVQRMRKGEL 850
    WHGIFTMIQM EQMHSRIIKT LSITLGVKLD KKDRLLEELM ACNHVANFSV 900
    EDIDELNRNI KKEIQISSGL KPPVNTIDLT NGEPFGNAVP TFTKTWSSSL 950
    DNLEKLSSAA AVGQSLDYNS GLRQGPLAGG GSKEQTPIAG MNNLNNSINA 1000
    TPIVDNSSGS QLPNGFDRGQ ANNTPFPGYF GGLDLFDYDF LFGNDFA 1047
    Length:1,047
    Mass (Da):118,891
    Last modified:July 27, 2011 - v3
    Checksum:i7D9388450486F540
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti70 – 701R → W in AAC04981. (PubMed:7731988)Curated
    Sequence conflicti447 – 4471Q → P in AAC04981. (PubMed:7731988)Curated
    Sequence conflicti588 – 5881K → T in AAC04981. (PubMed:7731988)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12980 Genomic DNA. Translation: AAC04981.2.
    BK006935 Genomic DNA. Translation: DAA06936.2.
    PIRiS51969.
    RefSeqiNP_009349.3. NM_001178195.2.

    Genome annotation databases

    EnsemblFungiiYAL051W; YAL051W; YAL051W.
    GeneIDi851247.
    KEGGisce:YAL051W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12980 Genomic DNA. Translation: AAC04981.2 .
    BK006935 Genomic DNA. Translation: DAA06936.2 .
    PIRi S51969.
    RefSeqi NP_009349.3. NM_001178195.2.

    3D structure databases

    ProteinModelPortali P39720.
    SMRi P39720. Positions 57-133.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31777. 46 interactions.
    DIPi DIP-5928N.
    IntActi P39720. 4 interactions.
    MINTi MINT-2781292.
    STRINGi 4932.YAL051W.

    Proteomic databases

    MaxQBi P39720.
    PaxDbi P39720.
    PeptideAtlasi P39720.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL051W ; YAL051W ; YAL051W .
    GeneIDi 851247.
    KEGGi sce:YAL051W.

    Organism-specific databases

    CYGDi YAL051w.
    SGDi S000000048. OAF1.

    Phylogenomic databases

    eggNOGi NOG39949.
    GeneTreei ENSGT00390000000033.
    HOGENOMi HOG000066010.
    OMAi TKCDREK.
    OrthoDBi EOG7HF1SV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28857-MONOMER.

    Miscellaneous databases

    NextBioi 968186.

    Gene expression databases

    Genevestigatori P39720.

    Family and domain databases

    Gene3Di 4.10.240.10. 1 hit.
    InterProi IPR002409. Aflatoxin_BRP.
    IPR001138. Zn2-C6_fun-type_DNA-bd.
    [Graphical view ]
    Pfami PF00172. Zn_clus. 1 hit.
    [Graphical view ]
    PRINTSi PR00755. AFLATOXINBRP.
    SMARTi SM00066. GAL4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57701. SSF57701. 1 hit.
    PROSITEi PS00463. ZN2_CY6_FUNGAL_1. 1 hit.
    PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Hong E.L., Cherry J.M.
      Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO C-TERMINUS.
    3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 70; 447 AND 588.
      Strain: ATCC 204508 / S288c.
    4. "Purification, identification, and properties of a Saccharomyces cerevisiae oleate-activated upstream activating sequence-binding protein that is involved in the activation of POX1."
      Luo Y., Karpichev I.V., Kohanski R.A., Small G.M.
      J. Biol. Chem. 271:12068-12075(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 118-122; 946-953 AND 959-970, FUNCTION, DNA-BINDING.
    5. "A heterodimer of the Zn2Cys6 transcription factors Pip2p and Oaf1p controls induction of genes encoding peroxisomal proteins in Saccharomyces cerevisiae."
      Rottensteiner H., Kal A.J., Hamilton B., Ruis H., Tabak H.F.
      Eur. J. Biochem. 247:776-783(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH PIP2.
    6. "A complex containing two transcription factors regulates peroxisome proliferation and the coordinate induction of beta-oxidation enzymes in Saccharomyces cerevisiae."
      Karpichev I.V., Luo Y., Marians R.C., Small G.M.
      Mol. Cell. Biol. 17:69-80(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PIP2, IDENTIFICATION OF FRAMESHIFT.
    7. "Functional analysis of the Zn(2)Cys(6) transcription factors Oaf1p and Pip2p. Different roles in fatty acid induction of beta-oxidation in Saccharomyces cerevisiae."
      Baumgartner U., Hamilton B., Piskacek M., Ruis H., Rottensteiner H.
      J. Biol. Chem. 274:22208-22216(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Saccharomyces cerevisiae Pip2p-Oaf1p regulates PEX25 transcription through an adenine-less ORE."
      Rottensteiner H., Hartig A., Hamilton B., Ruis H., Erdmann R., Gurvitz A.
      Eur. J. Biochem. 270:2013-2022(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA-BINDING.
    9. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
      Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
      Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF FRAMESHIFT.
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "Nine-amino-acid transactivation domain: establishment and prediction utilities."
      Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M.
      Genomics 89:756-768(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiOAF1_YEAST
    AccessioniPrimary (citable) accession number: P39720
    Secondary accession number(s): D6VPG6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 92 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3