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P39720

- OAF1_YEAST

UniProt

P39720 - OAF1_YEAST

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Protein

Oleate-activated transcription factor 1

Gene

OAF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The PIP2-OAF1 heterodimer acts as a transcriptional activator to induce the transcription of genes encoding proteins involved in fatty acid beta-oxidation, a response called oleic acid induction, when cells grow on fatty acids as sole carbon source. Recognizes and binds to the oleate response element (ORE) (or peroxisome box), two inverted CGG triplets spaced by 14 to 18 intervening nucleotides, in the promoter region of a number of genes (such as CTA1, FOX1 to FOX3, FAA2, PAS8, PAS10, etc.) for peroxisomal proteins. OAF1 acts as the sensor for oleate and inhibits PIP2 activity under non-inducing conditions. Activity is repressed by glucose.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi66 – 9328Zn(2)-C6 fungal-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: SGD
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. cellular response to oleic acid Source: SGD
  2. positive regulation of chromatin silencing at telomere Source: SGD
  3. positive regulation of fatty acid beta-oxidation Source: SGD
  4. positive regulation of transcription from RNA polymerase II promoter Source: SGD
  5. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Sporulation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-28857-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Oleate-activated transcription factor 1
Gene namesi
Name:OAF1
Synonyms:YAF1
Ordered Locus Names:YAL051W
ORF Names:FUN43
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL051w.
SGDiS000000048. OAF1.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10471047Oleate-activated transcription factor 1PRO_0000114990Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei155 – 1551Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39720.
PaxDbiP39720.
PeptideAtlasiP39720.

Expressioni

Gene expression databases

GenevestigatoriP39720.

Interactioni

Subunit structurei

Heterodimer of PIP2 and OAF1.

Protein-protein interaction databases

BioGridi31777. 46 interactions.
DIPiDIP-5928N.
IntActiP39720. 4 interactions.
MINTiMINT-2781292.
STRINGi4932.YAL051W.

Structurei

3D structure databases

ProteinModelPortaliP39720.
SMRiP39720. Positions 57-133.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1034 – 104299aaTAD

Domaini

the 9aaTAD motif (residues 1034 to 1042) is a transactivation domain present in a large number of yeast and animal transcription factors.1 Publication

Sequence similaritiesi

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG39949.
GeneTreeiENSGT00390000000033.
HOGENOMiHOG000066010.
InParanoidiP39720.
OMAiTKCDREK.
OrthoDBiEOG7HF1SV.

Family and domain databases

Gene3Di4.10.240.10. 1 hit.
InterProiIPR002409. Aflatoxin_BRP.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamiPF00172. Zn_clus. 1 hit.
[Graphical view]
PRINTSiPR00755. AFLATOXINBRP.
SMARTiSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMiSSF57701. SSF57701. 1 hit.
PROSITEiPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39720-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVENSTQKAP HAGNDDNSST KPYSEAFFLG FNNPTPGLEA EHSSTSPAPE
60 70 80 90 100
NSETHNRKRN RILFVCQACR KSKTKCDREK PECGRCVKHG LKCVYDVSKQ
110 120 130 140 150
PAPRIPSKDA IISRLEKDMF YWKDKAMKLL TEREVNESGK RSASPINTNN
160 170 180 190 200
ASGDSPDTKK QHKMEPIYEQ SGNGDINNGT RNDIEINLYR SHPTMIMSKV
210 220 230 240 250
MKREVKPLSE NYIIIQDCFL KILVTSVFLD TSKNTMIPAL TANANITRAQ
260 270 280 290 300
PSVANNLLKL KEMLIRQCQT EDEKNRVNEF TDRILQNTNS NRNLKIGMLL
310 320 330 340 350
SMLYNSVGYQ YLEDHCPQGG EYSDLLRNLI NECEAILPSY EIIERYKNHF
360 370 380 390 400
YEYVYPSLPF IELEIFEESL SQTIFPDPNN PSKVQIRMGS THLRAKVENL
410 420 430 440 450
SLLLVILKLS YMSIRFLDHS TADSSFYLSK EIIDKYPIPN DFILLSQRCL
460 470 480 490 500
ASENWCACAN ENIISCLLYI WSFFAFSPEE GDFFLEHPTD VISSLIMMLS
510 520 530 540 550
TSIGLHRDPS DFPQLISPST SDKRTLNHRR ILWLSIVTVC SFEASLKGRH
560 570 580 590 600
SVSPISLMAL FLNIKDPDSL TVYMNRVRGD LSDINNHKLL RIHKFTFKRA
610 620 630 640 650
QLALLLSDLD NLTMTYYGSF HLHSIEFIRE KIEIFVEENF PIVPLKSVAQ
660 670 680 690 700
DKSDLDDMNV ISEMNILSSE NSSSFHNRIM NKLLMLRTSM AVFLHFETLI
710 720 730 740 750
TKDKSIFPFY KKYFMVSCMD ALSLINYFNK FFNGEYRHAI SSLTSFNVTK
760 770 780 790 800
FIQLALSSTI FSLLGIILRI GLAIHMLSSE VQKLSGTTDP RIKELNTKVE
810 820 830 840 850
KFSTLQRDLE SALEGIYCSA SEHLRFTYFP VFKMLALFDV IVQRMRKGEL
860 870 880 890 900
WHGIFTMIQM EQMHSRIIKT LSITLGVKLD KKDRLLEELM ACNHVANFSV
910 920 930 940 950
EDIDELNRNI KKEIQISSGL KPPVNTIDLT NGEPFGNAVP TFTKTWSSSL
960 970 980 990 1000
DNLEKLSSAA AVGQSLDYNS GLRQGPLAGG GSKEQTPIAG MNNLNNSINA
1010 1020 1030 1040
TPIVDNSSGS QLPNGFDRGQ ANNTPFPGYF GGLDLFDYDF LFGNDFA
Length:1,047
Mass (Da):118,891
Last modified:July 27, 2011 - v3
Checksum:i7D9388450486F540
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701R → W in AAC04981. (PubMed:7731988)Curated
Sequence conflicti447 – 4471Q → P in AAC04981. (PubMed:7731988)Curated
Sequence conflicti588 – 5881K → T in AAC04981. (PubMed:7731988)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12980 Genomic DNA. Translation: AAC04981.2.
BK006935 Genomic DNA. Translation: DAA06936.2.
PIRiS51969.
RefSeqiNP_009349.3. NM_001178195.2.

Genome annotation databases

EnsemblFungiiYAL051W; YAL051W; YAL051W.
GeneIDi851247.
KEGGisce:YAL051W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12980 Genomic DNA. Translation: AAC04981.2 .
BK006935 Genomic DNA. Translation: DAA06936.2 .
PIRi S51969.
RefSeqi NP_009349.3. NM_001178195.2.

3D structure databases

ProteinModelPortali P39720.
SMRi P39720. Positions 57-133.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31777. 46 interactions.
DIPi DIP-5928N.
IntActi P39720. 4 interactions.
MINTi MINT-2781292.
STRINGi 4932.YAL051W.

Proteomic databases

MaxQBi P39720.
PaxDbi P39720.
PeptideAtlasi P39720.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL051W ; YAL051W ; YAL051W .
GeneIDi 851247.
KEGGi sce:YAL051W.

Organism-specific databases

CYGDi YAL051w.
SGDi S000000048. OAF1.

Phylogenomic databases

eggNOGi NOG39949.
GeneTreei ENSGT00390000000033.
HOGENOMi HOG000066010.
InParanoidi P39720.
OMAi TKCDREK.
OrthoDBi EOG7HF1SV.

Enzyme and pathway databases

BioCyci YEAST:G3O-28857-MONOMER.

Miscellaneous databases

NextBioi 968186.

Gene expression databases

Genevestigatori P39720.

Family and domain databases

Gene3Di 4.10.240.10. 1 hit.
InterProi IPR002409. Aflatoxin_BRP.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view ]
Pfami PF00172. Zn_clus. 1 hit.
[Graphical view ]
PRINTSi PR00755. AFLATOXINBRP.
SMARTi SM00066. GAL4. 1 hit.
[Graphical view ]
SUPFAMi SSF57701. SSF57701. 1 hit.
PROSITEi PS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Hong E.L., Cherry J.M.
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 70; 447 AND 588.
    Strain: ATCC 204508 / S288c.
  4. "Purification, identification, and properties of a Saccharomyces cerevisiae oleate-activated upstream activating sequence-binding protein that is involved in the activation of POX1."
    Luo Y., Karpichev I.V., Kohanski R.A., Small G.M.
    J. Biol. Chem. 271:12068-12075(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 118-122; 946-953 AND 959-970, FUNCTION, DNA-BINDING.
  5. "A heterodimer of the Zn2Cys6 transcription factors Pip2p and Oaf1p controls induction of genes encoding peroxisomal proteins in Saccharomyces cerevisiae."
    Rottensteiner H., Kal A.J., Hamilton B., Ruis H., Tabak H.F.
    Eur. J. Biochem. 247:776-783(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH PIP2.
  6. "A complex containing two transcription factors regulates peroxisome proliferation and the coordinate induction of beta-oxidation enzymes in Saccharomyces cerevisiae."
    Karpichev I.V., Luo Y., Marians R.C., Small G.M.
    Mol. Cell. Biol. 17:69-80(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PIP2, IDENTIFICATION OF FRAMESHIFT.
  7. "Functional analysis of the Zn(2)Cys(6) transcription factors Oaf1p and Pip2p. Different roles in fatty acid induction of beta-oxidation in Saccharomyces cerevisiae."
    Baumgartner U., Hamilton B., Piskacek M., Ruis H., Rottensteiner H.
    J. Biol. Chem. 274:22208-22216(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Saccharomyces cerevisiae Pip2p-Oaf1p regulates PEX25 transcription through an adenine-less ORE."
    Rottensteiner H., Hartig A., Hamilton B., Ruis H., Erdmann R., Gurvitz A.
    Eur. J. Biochem. 270:2013-2022(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA-BINDING.
  9. "Sequencing and comparison of yeast species to identify genes and regulatory elements."
    Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
    Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF FRAMESHIFT.
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Nine-amino-acid transactivation domain: establishment and prediction utilities."
    Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M.
    Genomics 89:756-768(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiOAF1_YEAST
AccessioniPrimary (citable) accession number: P39720
Secondary accession number(s): D6VPG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 92 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3