Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P39720 (OAF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Oleate-activated transcription factor 1
Gene names
Name:OAF1
Synonyms:YAF1
Ordered Locus Names:YAL051W
ORF Names:FUN43
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1047 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The PIP2-OAF1 heterodimer acts as a transcriptional activator to induce the transcription of genes encoding proteins involved in fatty acid beta-oxidation, a response called oleic acid induction, when cells grow on fatty acids as sole carbon source. Recognizes and binds to the oleate response element (ORE) (or peroxisome box), two inverted CGG triplets spaced by 14 to 18 intervening nucleotides, in the promoter region of a number of genes (such as CTA1, FOX1 to FOX3, FAA2, PAS8, PAS10, etc.) for peroxisomal proteins. OAF1 acts as the sensor for oleate and inhibits PIP2 activity under non-inducing conditions. Activity is repressed by glucose. Ref.4 Ref.5 Ref.6 Ref.7

Subunit structure

Heterodimer of PIP2 and OAF1.

Subcellular location

Nucleus.

Domain

the 9aaTAD motif (residues 1034 to 1042) is a transactivation domain present in a large number of yeast and animal transcription factors. Ref.11

Miscellaneous

Present with 92 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10471047Oleate-activated transcription factor 1
PRO_0000114990

Regions

DNA binding66 – 9328Zn(2)-C6 fungal-type Ref.4 Ref.5 Ref.8
Motif1034 – 104299aaTAD

Amino acid modifications

Modified residue1551Phosphoserine Ref.13

Experimental info

Sequence conflict701R → W in AAC04981. Ref.1
Sequence conflict4471Q → P in AAC04981. Ref.1
Sequence conflict5881K → T in AAC04981. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P39720 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 7D9388450486F540

FASTA1,047118,891
        10         20         30         40         50         60 
MVENSTQKAP HAGNDDNSST KPYSEAFFLG FNNPTPGLEA EHSSTSPAPE NSETHNRKRN 

        70         80         90        100        110        120 
RILFVCQACR KSKTKCDREK PECGRCVKHG LKCVYDVSKQ PAPRIPSKDA IISRLEKDMF 

       130        140        150        160        170        180 
YWKDKAMKLL TEREVNESGK RSASPINTNN ASGDSPDTKK QHKMEPIYEQ SGNGDINNGT 

       190        200        210        220        230        240 
RNDIEINLYR SHPTMIMSKV MKREVKPLSE NYIIIQDCFL KILVTSVFLD TSKNTMIPAL 

       250        260        270        280        290        300 
TANANITRAQ PSVANNLLKL KEMLIRQCQT EDEKNRVNEF TDRILQNTNS NRNLKIGMLL 

       310        320        330        340        350        360 
SMLYNSVGYQ YLEDHCPQGG EYSDLLRNLI NECEAILPSY EIIERYKNHF YEYVYPSLPF 

       370        380        390        400        410        420 
IELEIFEESL SQTIFPDPNN PSKVQIRMGS THLRAKVENL SLLLVILKLS YMSIRFLDHS 

       430        440        450        460        470        480 
TADSSFYLSK EIIDKYPIPN DFILLSQRCL ASENWCACAN ENIISCLLYI WSFFAFSPEE 

       490        500        510        520        530        540 
GDFFLEHPTD VISSLIMMLS TSIGLHRDPS DFPQLISPST SDKRTLNHRR ILWLSIVTVC 

       550        560        570        580        590        600 
SFEASLKGRH SVSPISLMAL FLNIKDPDSL TVYMNRVRGD LSDINNHKLL RIHKFTFKRA 

       610        620        630        640        650        660 
QLALLLSDLD NLTMTYYGSF HLHSIEFIRE KIEIFVEENF PIVPLKSVAQ DKSDLDDMNV 

       670        680        690        700        710        720 
ISEMNILSSE NSSSFHNRIM NKLLMLRTSM AVFLHFETLI TKDKSIFPFY KKYFMVSCMD 

       730        740        750        760        770        780 
ALSLINYFNK FFNGEYRHAI SSLTSFNVTK FIQLALSSTI FSLLGIILRI GLAIHMLSSE 

       790        800        810        820        830        840 
VQKLSGTTDP RIKELNTKVE KFSTLQRDLE SALEGIYCSA SEHLRFTYFP VFKMLALFDV 

       850        860        870        880        890        900 
IVQRMRKGEL WHGIFTMIQM EQMHSRIIKT LSITLGVKLD KKDRLLEELM ACNHVANFSV 

       910        920        930        940        950        960 
EDIDELNRNI KKEIQISSGL KPPVNTIDLT NGEPFGNAVP TFTKTWSSSL DNLEKLSSAA 

       970        980        990       1000       1010       1020 
AVGQSLDYNS GLRQGPLAGG GSKEQTPIAG MNNLNNSINA TPIVDNSSGS QLPNGFDRGQ 

      1030       1040 
ANNTPFPGYF GGLDLFDYDF LFGNDFA 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Hong E.L., Cherry J.M.
Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 70; 447 AND 588.
Strain: ATCC 204508 / S288c.
[4]"Purification, identification, and properties of a Saccharomyces cerevisiae oleate-activated upstream activating sequence-binding protein that is involved in the activation of POX1."
Luo Y., Karpichev I.V., Kohanski R.A., Small G.M.
J. Biol. Chem. 271:12068-12075(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 118-122; 946-953 AND 959-970, FUNCTION, DNA-BINDING.
[5]"A heterodimer of the Zn2Cys6 transcription factors Pip2p and Oaf1p controls induction of genes encoding peroxisomal proteins in Saccharomyces cerevisiae."
Rottensteiner H., Kal A.J., Hamilton B., Ruis H., Tabak H.F.
Eur. J. Biochem. 247:776-783(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH PIP2.
[6]"A complex containing two transcription factors regulates peroxisome proliferation and the coordinate induction of beta-oxidation enzymes in Saccharomyces cerevisiae."
Karpichev I.V., Luo Y., Marians R.C., Small G.M.
Mol. Cell. Biol. 17:69-80(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PIP2, IDENTIFICATION OF FRAMESHIFT.
[7]"Functional analysis of the Zn(2)Cys(6) transcription factors Oaf1p and Pip2p. Different roles in fatty acid induction of beta-oxidation in Saccharomyces cerevisiae."
Baumgartner U., Hamilton B., Piskacek M., Ruis H., Rottensteiner H.
J. Biol. Chem. 274:22208-22216(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Saccharomyces cerevisiae Pip2p-Oaf1p regulates PEX25 transcription through an adenine-less ORE."
Rottensteiner H., Hartig A., Hamilton B., Ruis H., Erdmann R., Gurvitz A.
Eur. J. Biochem. 270:2013-2022(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA-BINDING.
[9]"Sequencing and comparison of yeast species to identify genes and regulatory elements."
Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.
Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF FRAMESHIFT.
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"Nine-amino-acid transactivation domain: establishment and prediction utilities."
Piskacek S., Gregor M., Nemethova M., Grabner M., Kovarik P., Piskacek M.
Genomics 89:756-768(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAIN.
[12]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-155, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12980 Genomic DNA. Translation: AAC04981.2.
BK006935 Genomic DNA. Translation: DAA06936.2.
PIRS51969.
RefSeqNP_009349.3. NM_001178195.2.

3D structure databases

ProteinModelPortalP39720.
SMRP39720. Positions 57-133.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31777. 45 interactions.
DIPDIP-5928N.
IntActP39720. 4 interactions.
MINTMINT-2781292.
STRING4932.YAL051W.

Proteomic databases

MaxQBP39720.
PaxDbP39720.
PeptideAtlasP39720.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAL051W; YAL051W; YAL051W.
GeneID851247.
KEGGsce:YAL051W.

Organism-specific databases

CYGDYAL051w.
SGDS000000048. OAF1.

Phylogenomic databases

eggNOGNOG39949.
GeneTreeENSGT00390000000033.
HOGENOMHOG000066010.
OMATKCDREK.
OrthoDBEOG7HF1SV.

Enzyme and pathway databases

BioCycYEAST:G3O-28857-MONOMER.

Gene expression databases

GenevestigatorP39720.

Family and domain databases

Gene3D4.10.240.10. 1 hit.
InterProIPR002409. Aflatoxin_BRP.
IPR001138. Zn2-C6_fun-type_DNA-bd.
[Graphical view]
PfamPF00172. Zn_clus. 1 hit.
[Graphical view]
PRINTSPR00755. AFLATOXINBRP.
SMARTSM00066. GAL4. 1 hit.
[Graphical view]
SUPFAMSSF57701. SSF57701. 1 hit.
PROSITEPS00463. ZN2_CY6_FUNGAL_1. 1 hit.
PS50048. ZN2_CY6_FUNGAL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968186.

Entry information

Entry nameOAF1_YEAST
AccessionPrimary (citable) accession number: P39720
Secondary accession number(s): D6VPG6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: May 14, 2014
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families