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P39715

- ECM1_YEAST

UniProt

P39715 - ECM1_YEAST

Protein

Shuttling pre-60S factor ECM1

Gene

ECM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Pre-ribosomal factor involved in 60S ribosomal protein subunit export from the nucleus.2 Publications

    GO - Biological processi

    1. ribosomal large subunit export from nucleus Source: SGD

    Keywords - Biological processi

    Ribosome biogenesis, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28862-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Shuttling pre-60S factor ECM1
    Alternative name(s):
    Extracellular mutant protein 1
    Protein SIM1
    Gene namesi
    Name:ECM1
    Synonyms:SIM1
    Ordered Locus Names:YAL059W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAL059w.
    SGDiS000000055. ECM1.

    Subcellular locationi

    Nucleusnucleolus. Nucleus. Cytoplasm
    Note: Shuttles between the nucleus and the cytoplasm and is re-imported by the KAP123 karyopherin.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleolus Source: SGD
    3. nucleus Source: SGD
    4. preribosome, large subunit precursor Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 212212Shuttling pre-60S factor ECM1PRO_0000086916Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei188 – 1881Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39715.
    PaxDbiP39715.
    PeptideAtlasiP39715.

    Expressioni

    Gene expression databases

    GenevestigatoriP39715.

    Interactioni

    Subunit structurei

    Associates with the pre-60S ribosomal particle and the nucleopore complex.

    Protein-protein interaction databases

    BioGridi31771. 65 interactions.
    DIPiDIP-2465N.
    IntActiP39715. 21 interactions.
    MINTiMINT-621124.
    STRINGi4932.YAL059W.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ECM1 family.Curated

    Phylogenomic databases

    eggNOGiNOG44019.
    HOGENOMiHOG000094086.
    OMAiQCVISAK.
    OrthoDBiEOG7F255B.

    Family and domain databases

    InterProiIPR022784. Ribosome_bgen_Alb1.
    [Graphical view]
    PfamiPF09135. Alb1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39715-1 [UniParc]FASTAAdd to Basket

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    MWEQRRQKVV FSLTILVRYR LKQSMAKKIS KNSRAARQSD ALEPEVKDLS    50
    ELPRAEKTDL TNILIRTAAK NEALLEAKIS KKANKSKRGK KLNKKALEDK 100
    LANSISSMDR DRLVKALNFT NRLDGKIAKS ISRAKYIQNT RKAGWDSTNE 150
    TIKKELAFLN GGLSVQAKSA SEGNAEKEDE EIPEVFDSLA EDNTVQKTPT 200
    NRFGVLPDDV EE 212
    Length:212
    Mass (Da):23,891
    Last modified:July 27, 2011 - v2
    Checksum:iACFFE6CFF6F62CD5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti102 – 1021A → D in AAC04975. (PubMed:7731988)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12980 Genomic DNA. Translation: AAC04975.1.
    AY558168 Genomic DNA. Translation: AAS56494.1.
    BK006935 Genomic DNA. Translation: DAA06930.2.
    PIRiS51963.
    RefSeqiNP_009342.2. NM_001178201.2.

    Genome annotation databases

    EnsemblFungiiYAL059W; YAL059W; YAL059W.
    GeneIDi851240.
    KEGGisce:YAL059W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12980 Genomic DNA. Translation: AAC04975.1 .
    AY558168 Genomic DNA. Translation: AAS56494.1 .
    BK006935 Genomic DNA. Translation: DAA06930.2 .
    PIRi S51963.
    RefSeqi NP_009342.2. NM_001178201.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31771. 65 interactions.
    DIPi DIP-2465N.
    IntActi P39715. 21 interactions.
    MINTi MINT-621124.
    STRINGi 4932.YAL059W.

    Proteomic databases

    MaxQBi P39715.
    PaxDbi P39715.
    PeptideAtlasi P39715.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL059W ; YAL059W ; YAL059W .
    GeneIDi 851240.
    KEGGi sce:YAL059W.

    Organism-specific databases

    CYGDi YAL059w.
    SGDi S000000055. ECM1.

    Phylogenomic databases

    eggNOGi NOG44019.
    HOGENOMi HOG000094086.
    OMAi QCVISAK.
    OrthoDBi EOG7F255B.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28862-MONOMER.

    Miscellaneous databases

    NextBioi 968168.

    Gene expression databases

    Genevestigatori P39715.

    Family and domain databases

    InterProi IPR022784. Ribosome_bgen_Alb1.
    [Graphical view ]
    Pfami PF09135. Alb1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 102.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Identification of a 60S preribosomal particle that is closely linked to nuclear export."
      Bassler J., Grandi P., Gadal O., Lessmann T., Petfalski E., Tollervey D., Lechner J., Hurt E.
      Mol. Cell 8:517-529(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Ecm1 is a new pre-ribosomal factor involved in pre-60S particle export."
      Yao Y., Demoinet E., Saveanu C., Lenormand P., Jacquier A., Fromont-Racine M.
      RNA 16:1007-1017(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, ASSOCIATION WITH THE PRE-60S PARTICLE AND THE NUCLEOPORE COMPLEX.

    Entry informationi

    Entry nameiECM1_YEAST
    AccessioniPrimary (citable) accession number: P39715
    Secondary accession number(s): D6VPG0, E9P8V4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2840 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3