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P39715 (ECM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Shuttling pre-60S factor ECM1
Alternative name(s):
Extracellular mutant protein 1
Protein SIM1
Gene names
Name:ECM1
Synonyms:SIM1
Ordered Locus Names:YAL059W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length212 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Pre-ribosomal factor involved in 60S ribosomal protein subunit export from the nucleus. Ref.4 Ref.8

Subunit structure

Associates with the pre-60S ribosomal particle and the nucleopore complex.

Subcellular location

Nucleusnucleolus. Nucleus. Cytoplasm. Note: Shuttles between the nucleus and the cytoplasm and is re-imported by the KAP123 karyopherin. Ref.4 Ref.5 Ref.8

Miscellaneous

Present with 2840 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ECM1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 212212Shuttling pre-60S factor ECM1
PRO_0000086916

Amino acid modifications

Modified residue1881Phosphoserine Ref.7

Experimental info

Sequence conflict1021A → D in AAC04975. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P39715 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: ACFFE6CFF6F62CD5

FASTA21223,891
        10         20         30         40         50         60 
MWEQRRQKVV FSLTILVRYR LKQSMAKKIS KNSRAARQSD ALEPEVKDLS ELPRAEKTDL 

        70         80         90        100        110        120 
TNILIRTAAK NEALLEAKIS KKANKSKRGK KLNKKALEDK LANSISSMDR DRLVKALNFT 

       130        140        150        160        170        180 
NRLDGKIAKS ISRAKYIQNT RKAGWDSTNE TIKKELAFLN GGLSVQAKSA SEGNAEKEDE 

       190        200        210 
EIPEVFDSLA EDNTVQKTPT NRFGVLPDDV EE 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 102.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Identification of a 60S preribosomal particle that is closely linked to nuclear export."
Bassler J., Grandi P., Gadal O., Lessmann T., Petfalski E., Tollervey D., Lechner J., Hurt E.
Mol. Cell 8:517-529(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Ecm1 is a new pre-ribosomal factor involved in pre-60S particle export."
Yao Y., Demoinet E., Saveanu C., Lenormand P., Jacquier A., Fromont-Racine M.
RNA 16:1007-1017(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, ASSOCIATION WITH THE PRE-60S PARTICLE AND THE NUCLEOPORE COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12980 Genomic DNA. Translation: AAC04975.1.
AY558168 Genomic DNA. Translation: AAS56494.1.
BK006935 Genomic DNA. Translation: DAA06930.2.
PIRS51963.
RefSeqNP_009342.2. NM_001178201.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31771. 65 interactions.
DIPDIP-2465N.
IntActP39715. 21 interactions.
MINTMINT-621124.
STRING4932.YAL059W.

Proteomic databases

MaxQBP39715.
PaxDbP39715.
PeptideAtlasP39715.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAL059W; YAL059W; YAL059W.
GeneID851240.
KEGGsce:YAL059W.

Organism-specific databases

CYGDYAL059w.
SGDS000000055. ECM1.

Phylogenomic databases

eggNOGNOG44019.
HOGENOMHOG000094086.
OMAQCVISAK.
OrthoDBEOG7F255B.

Enzyme and pathway databases

BioCycYEAST:G3O-28862-MONOMER.

Gene expression databases

GenevestigatorP39715.

Family and domain databases

InterProIPR022784. Ribosome_bgen_Alb1.
[Graphical view]
PfamPF09135. Alb1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968168.

Entry information

Entry nameECM1_YEAST
AccessionPrimary (citable) accession number: P39715
Secondary accession number(s): D6VPG0, E9P8V4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: May 14, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families