P39715 (ECM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shuttling pre-60S factor ECM1

Alternative name(s):
Extracellular mutant protein 1
Protein SIM1

Gene names

Name:	ECM1
Synonyms:	SIM1
Ordered Locus Names:	YAL059W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Pre-ribosomal factor involved in 60S ribosomal protein subunit export from the nucleus. Ref.4 Ref.8
Subunit structure	Associates with the pre-60S ribosomal particle and the nucleopore complex.
Subcellular location	Nucleus › nucleolus. Nucleus. Cytoplasm. Note: Shuttles between the nucleus and the cytoplasm and is re-imported by the KAP123 karyopherin. Ref.4 Ref.5 Ref.8
Miscellaneous	Present with 2840 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the ECM1 family.

Ontologies

Keywords
Biological process	Ribosome biogenesis Transport
Cellular component	Cytoplasm Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ribosomal large subunit export from nucleus Inferred from genetic interaction Ref.4Ref.8. Source: SGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from direct assay Ref.4. Source: SGD preribosome, large subunit precursor Inferred from direct assay Ref.8. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Shuttling pre-60S factor ECM1		PRO_0000086916
Amino acid modifications
	Modified residue	188	1	Phosphoserine Ref.7
Experimental info
	Sequence conflict	102	1	A → D in AAC04975. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P39715 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: ACFFE6CFF6F62CD5	FASTA	212	23,891
	10 20 30 40 50 60 MWEQRRQKVV FSLTILVRYR LKQSMAKKIS KNSRAARQSD ALEPEVKDLS ELPRAEKTDL 70 80 90 100 110 120 TNILIRTAAK NEALLEAKIS KKANKSKRGK KLNKKALEDK LANSISSMDR DRLVKALNFT 130 140 150 160 170 180 NRLDGKIAKS ISRAKYIQNT RKAGWDSTNE TIKKELAFLN GGLSVQAKSA SEGNAEKEDE 190 200 210 EIPEVFDSLA EDNTVQKTPT NRFGVLPDDV EE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 102. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Identification of a 60S preribosomal particle that is closely linked to nuclear export." Bassler J., Grandi P., Gadal O., Lessmann T., Petfalski E., Tollervey D., Lechner J., Hurt E. Mol. Cell 8:517-529(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188, MASS SPECTROMETRY.
[8]	"Ecm1 is a new pre-ribosomal factor involved in pre-60S particle export." Yao Y., Demoinet E., Saveanu C., Lenormand P., Jacquier A., Fromont-Racine M. RNA 16:1007-1017(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, ASSOCIATION WITH THE PRE-60S PARTICLE AND THE NUCLEOPORE COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12980 Genomic DNA. Translation: AAC04975.1. AY558168 Genomic DNA. Translation: AAS56494.1. BK006935 Genomic DNA. Translation: DAA06930.2.
PIR	S51963.
RefSeq	NP_009342.2. NM_001178201.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2465N.
IntAct	P39715. 21 interactions.
MINT	MINT-621124.
STRING	4932.YAL059W.
Proteomic databases
PaxDb	P39715.
PeptideAtlas	P39715.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL059W; YAL059W; YAL059W.
GeneID	851240.
KEGG	sce:YAL059W.
Organism-specific databases
CYGD	YAL059w.
SGD	S000000055. ECM1.
Phylogenomic databases
eggNOG	NOG44019.
HOGENOM	HOG000094086.
OMA	RKAGWDS.
OrthoDB	EOG4004V0.
Enzyme and pathway databases
BioCyc	YEAST:G3O-28862-MONOMER.
Gene expression databases
Genevestigator	P39715.
GermOnline	YAL059W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR022784. Ribosome_bgen_Alb1. [Graphical view]
Pfam	PF09135. Alb1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968168.

Entry information

Entry name

ECM1_YEAST

Accession

Primary (citable) accession number: P39715
Secondary accession number(s): D6VPG0, E9P8V4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	July 27, 2011
Last modified:	May 29, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program