Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Shuttling pre-60S factor ECM1

Gene

ECM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pre-ribosomal factor involved in 60S ribosomal protein subunit export from the nucleus.2 Publications

GO - Biological processi

  1. ribosomal large subunit export from nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-28862-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Shuttling pre-60S factor ECM1
Alternative name(s):
Extracellular mutant protein 1
Protein SIM1
Gene namesi
Name:ECM1
Synonyms:SIM1
Ordered Locus Names:YAL059W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL059w.
SGDiS000000055. ECM1.

Subcellular locationi

Nucleusnucleolus. Nucleus. Cytoplasm
Note: Shuttles between the nucleus and the cytoplasm and is re-imported by the KAP123 karyopherin.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleolus Source: SGD
  3. nucleus Source: SGD
  4. preribosome, large subunit precursor Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 212212Shuttling pre-60S factor ECM1PRO_0000086916Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei188 – 1881Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39715.
PaxDbiP39715.
PeptideAtlasiP39715.

Expressioni

Gene expression databases

GenevestigatoriP39715.

Interactioni

Subunit structurei

Associates with the pre-60S ribosomal particle and the nucleopore complex.

Protein-protein interaction databases

BioGridi31771. 66 interactions.
DIPiDIP-2465N.
IntActiP39715. 21 interactions.
MINTiMINT-621124.
STRINGi4932.YAL059W.

Family & Domainsi

Sequence similaritiesi

Belongs to the ECM1 family.Curated

Phylogenomic databases

eggNOGiNOG44019.
HOGENOMiHOG000094086.
InParanoidiP39715.
OMAiRKAGWDS.
OrthoDBiEOG7F255B.

Family and domain databases

InterProiIPR022784. Ribosome_bgen_Alb1.
[Graphical view]
PfamiPF09135. Alb1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39715-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWEQRRQKVV FSLTILVRYR LKQSMAKKIS KNSRAARQSD ALEPEVKDLS
60 70 80 90 100
ELPRAEKTDL TNILIRTAAK NEALLEAKIS KKANKSKRGK KLNKKALEDK
110 120 130 140 150
LANSISSMDR DRLVKALNFT NRLDGKIAKS ISRAKYIQNT RKAGWDSTNE
160 170 180 190 200
TIKKELAFLN GGLSVQAKSA SEGNAEKEDE EIPEVFDSLA EDNTVQKTPT
210
NRFGVLPDDV EE
Length:212
Mass (Da):23,891
Last modified:July 27, 2011 - v2
Checksum:iACFFE6CFF6F62CD5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti102 – 1021A → D in AAC04975 (PubMed:7731988).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. Translation: AAC04975.1.
AY558168 Genomic DNA. Translation: AAS56494.1.
BK006935 Genomic DNA. Translation: DAA06930.2.
PIRiS51963.
RefSeqiNP_009342.2. NM_001178201.2.

Genome annotation databases

EnsemblFungiiYAL059W; YAL059W; YAL059W.
GeneIDi851240.
KEGGisce:YAL059W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. Translation: AAC04975.1.
AY558168 Genomic DNA. Translation: AAS56494.1.
BK006935 Genomic DNA. Translation: DAA06930.2.
PIRiS51963.
RefSeqiNP_009342.2. NM_001178201.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31771. 66 interactions.
DIPiDIP-2465N.
IntActiP39715. 21 interactions.
MINTiMINT-621124.
STRINGi4932.YAL059W.

Proteomic databases

MaxQBiP39715.
PaxDbiP39715.
PeptideAtlasiP39715.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL059W; YAL059W; YAL059W.
GeneIDi851240.
KEGGisce:YAL059W.

Organism-specific databases

CYGDiYAL059w.
SGDiS000000055. ECM1.

Phylogenomic databases

eggNOGiNOG44019.
HOGENOMiHOG000094086.
InParanoidiP39715.
OMAiRKAGWDS.
OrthoDBiEOG7F255B.

Enzyme and pathway databases

BioCyciYEAST:G3O-28862-MONOMER.

Miscellaneous databases

NextBioi968168.

Gene expression databases

GenevestigatoriP39715.

Family and domain databases

InterProiIPR022784. Ribosome_bgen_Alb1.
[Graphical view]
PfamiPF09135. Alb1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 102.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Identification of a 60S preribosomal particle that is closely linked to nuclear export."
    Bassler J., Grandi P., Gadal O., Lessmann T., Petfalski E., Tollervey D., Lechner J., Hurt E.
    Mol. Cell 8:517-529(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Ecm1 is a new pre-ribosomal factor involved in pre-60S particle export."
    Yao Y., Demoinet E., Saveanu C., Lenormand P., Jacquier A., Fromont-Racine M.
    RNA 16:1007-1017(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, ASSOCIATION WITH THE PRE-60S PARTICLE AND THE NUCLEOPORE COMPLEX.

Entry informationi

Entry nameiECM1_YEAST
AccessioniPrimary (citable) accession number: P39715
Secondary accession number(s): D6VPG0, E9P8V4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: January 7, 2015
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2840 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.