Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P39714

- BDH1_YEAST

UniProt

P39714 - BDH1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

(R,R)-butanediol dehydrogenase

Gene

BDH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

NAD-dependent (R,R)-butanediol dehydrogenase which catalyzes oxidation of (R,R)-butane-2,3-diol to (3R)-acetoin, of meso-butanediol to (3S)-acetoin, and reduction of acetoin. Allows the use of 2,3-butanediol as an aerobic carbon source.1 Publication

Catalytic activityi

(R,R)-butane-2,3-diol + NAD+ = (R)-acetoin + NADH.1 Publication

Cofactori

Binds 2 zinc ions per subunit.By similarity

Kineticsi

  1. KM=14 mM for (R,R)-butane-2,3-diol1 Publication
  2. KM=65 mM for meso-butanediol1 Publication
  3. KM=57 mM for 1,2-Butanediol1 Publication
  4. KM=4.5 mM for (3R/3S)-acetoin1 Publication
  5. KM=0.55 mM for NAD1 Publication
  6. KM=0.055 mM for NADH1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi39 – 391Zinc 1; catalyticBy similarity
Metal bindingi73 – 731Zinc 1; catalyticBy similarity
Metal bindingi103 – 1031Zinc 2By similarity
Metal bindingi120 – 1201Zinc 2By similarity
Metal bindingi123 – 1231Zinc 2By similarity
Metal bindingi131 – 1311Zinc 2By similarity
Metal bindingi173 – 1731Zinc 1; catalyticBy similarity

GO - Molecular functioni

  1. (R,R)-butanediol dehydrogenase activity Source: SGD
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. alcohol metabolic process Source: SGD
  2. butanediol biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14023.
YEAST:YAL060W-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
(R,R)-butanediol dehydrogenase (EC:1.1.1.4)
Gene namesi
Name:BDH1
Synonyms:BDH
Ordered Locus Names:YAL060W
ORF Names:FUN49
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL060w.
SGDiS000000056. BDH1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382(R,R)-butanediol dehydrogenasePRO_0000160788Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei63 – 631Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39714.
PaxDbiP39714.
PeptideAtlasiP39714.

Expressioni

Gene expression databases

GenevestigatoriP39714.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi31770. 34 interactions.
DIPiDIP-5356N.
IntActiP39714. 2 interactions.
MINTiMINT-487549.
STRINGi4932.YAL060W.

Structurei

3D structure databases

ProteinModelPortaliP39714.
SMRiP39714. Positions 1-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1063.
GeneTreeiENSGT00750000118226.
HOGENOMiHOG000294670.
InParanoidiP39714.
KOiK00004.
OMAiHEMSGTV.
OrthoDBiEOG7DVDNZ.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39714-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRALAYFKKG DIHFTNDIPR PEIQTDDEVI IDVSWCGICG SDLHEYLDGP
60 70 80 90 100
IFMPKDGECH KLSNAALPLA MGHEMSGIVS KVGPKVTKVK VGDHVVVDAA
110 120 130 140 150
SSCADLHCWP HSKFYNSKPC DACQRGSENL CTHAGFVGLG VISGGFAEQV
160 170 180 190 200
VVSQHHIIPV PKEIPLDVAA LVEPLSVTWH AVKISGFKKG SSALVLGAGP
210 220 230 240 250
IGLCTILVLK GMGASKIVVS EIAERRIEMA KKLGVEVFNP SKHGHKSIEI
260 270 280 290 300
LRGLTKSHDG FDYSYDCSGI QVTFETSLKA LTFKGTATNI AVWGPKPVPF
310 320 330 340 350
QPMDVTLQEK VMTGSIGYVV EDFEEVVRAI HNGDIAMEDC KQLITGKQRI
360 370 380
EDGWEKGFQE LMDHKESNVK ILLTPNNHGE MK
Length:382
Mass (Da):41,582
Last modified:July 27, 2011 - v2
Checksum:i1776FA5968EC1A12
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti322 – 3221D → A in AAC04974. (PubMed:7731988)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12980 Genomic DNA. Translation: AAC04974.1.
AY692922 Genomic DNA. Translation: AAT92941.1.
BK006935 Genomic DNA. Translation: DAA06929.2.
PIRiS51962.
RefSeqiNP_009341.2. NM_001178202.2.

Genome annotation databases

EnsemblFungiiYAL060W; YAL060W; YAL060W.
GeneIDi851239.
KEGGisce:YAL060W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12980 Genomic DNA. Translation: AAC04974.1 .
AY692922 Genomic DNA. Translation: AAT92941.1 .
BK006935 Genomic DNA. Translation: DAA06929.2 .
PIRi S51962.
RefSeqi NP_009341.2. NM_001178202.2.

3D structure databases

ProteinModelPortali P39714.
SMRi P39714. Positions 1-373.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31770. 34 interactions.
DIPi DIP-5356N.
IntActi P39714. 2 interactions.
MINTi MINT-487549.
STRINGi 4932.YAL060W.

Proteomic databases

MaxQBi P39714.
PaxDbi P39714.
PeptideAtlasi P39714.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL060W ; YAL060W ; YAL060W .
GeneIDi 851239.
KEGGi sce:YAL060W.

Organism-specific databases

CYGDi YAL060w.
SGDi S000000056. BDH1.

Phylogenomic databases

eggNOGi COG1063.
GeneTreei ENSGT00750000118226.
HOGENOMi HOG000294670.
InParanoidi P39714.
KOi K00004.
OMAi HEMSGTV.
OrthoDBi EOG7DVDNZ.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-14023.
YEAST:YAL060W-MONOMER.

Miscellaneous databases

NextBioi 968165.

Gene expression databases

Genevestigatori P39714.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProi IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11695. PTHR11695. 1 hit.
Pfami PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view ]
SUPFAMi SSF50129. SSF50129. 1 hit.
PROSITEi PS00059. ADH_ZINC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 322.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Characterization of a (2R,3R)-2,3-butanediol dehydrogenase as the Saccharomyces cerevisiae YAL060W gene product. Disruption and induction of the gene."
    Gonzalez E., Fernandez M.R., Larroy C., Sola L., Pericas M.A., Pares X., Biosca J.A.
    J. Biol. Chem. 275:35876-35885(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC 90845 / FY834.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBDH1_YEAST
AccessioniPrimary (citable) accession number: P39714
Secondary accession number(s): D6VPF9, E9P8Z3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8730 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3