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Protein

(R,R)-butanediol dehydrogenase

Gene

BDH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

NAD-dependent (R,R)-butanediol dehydrogenase which catalyzes oxidation of (R,R)-butane-2,3-diol to (3R)-acetoin, of meso-butanediol to (3S)-acetoin, and reduction of acetoin. Allows the use of 2,3-butanediol as an aerobic carbon source.1 Publication

Catalytic activityi

(R,R)-butane-2,3-diol + NAD+ = (R)-acetoin + NADH.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Kineticsi

  1. KM=14 mM for (R,R)-butane-2,3-diol1 Publication
  2. KM=65 mM for meso-butanediol1 Publication
  3. KM=57 mM for 1,2-Butanediol1 Publication
  4. KM=4.5 mM for (3R/3S)-acetoin1 Publication
  5. KM=0.55 mM for NAD1 Publication
  6. KM=0.055 mM for NADH1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi39 – 391Zinc 1; catalyticBy similarity
    Metal bindingi73 – 731Zinc 1; catalyticBy similarity
    Metal bindingi103 – 1031Zinc 2By similarity
    Metal bindingi120 – 1201Zinc 2By similarity
    Metal bindingi123 – 1231Zinc 2By similarity
    Metal bindingi131 – 1311Zinc 2By similarity
    Metal bindingi173 – 1731Zinc 1; catalyticBy similarity

    GO - Molecular functioni

    • (R,R)-butanediol dehydrogenase activity Source: SGD
    • zinc ion binding Source: InterPro

    GO - Biological processi

    • alcohol metabolic process Source: SGD
    • butanediol biosynthetic process Source: SGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Metal-binding, NAD, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14023.
    YEAST:YAL060W-MONOMER.
    BRENDAi1.1.1.303. 984.
    1.1.1.4. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    (R,R)-butanediol dehydrogenase (EC:1.1.1.4)
    Gene namesi
    Name:BDH1
    Synonyms:BDH
    Ordered Locus Names:YAL060W
    ORF Names:FUN49
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311 Componenti: Chromosome I

    Organism-specific databases

    CYGDiYAL060w.
    EuPathDBiFungiDB:YAL060W.
    SGDiS000000056. BDH1.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 382382(R,R)-butanediol dehydrogenasePRO_0000160788Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei63 – 631Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39714.
    PaxDbiP39714.
    PeptideAtlasiP39714.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi31770. 32 interactions.
    DIPiDIP-5356N.
    IntActiP39714. 2 interactions.
    MINTiMINT-487549.

    Structurei

    3D structure databases

    ProteinModelPortaliP39714.
    SMRiP39714. Positions 1-373.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1063.
    GeneTreeiENSGT00780000122413.
    HOGENOMiHOG000294670.
    InParanoidiP39714.
    KOiK00004.
    OMAiRARNHEN.
    OrthoDBiEOG7DVDNZ.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.180.10. 1 hit.
    InterProiIPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR002085. ADH_SF_Zn-type.
    IPR002328. ADH_Zn_CS.
    IPR011032. GroES-like.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11695. PTHR11695. 1 hit.
    PfamiPF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50129. SSF50129. 1 hit.
    PROSITEiPS00059. ADH_ZINC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39714-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRALAYFKKG DIHFTNDIPR PEIQTDDEVI IDVSWCGICG SDLHEYLDGP
    60 70 80 90 100
    IFMPKDGECH KLSNAALPLA MGHEMSGIVS KVGPKVTKVK VGDHVVVDAA
    110 120 130 140 150
    SSCADLHCWP HSKFYNSKPC DACQRGSENL CTHAGFVGLG VISGGFAEQV
    160 170 180 190 200
    VVSQHHIIPV PKEIPLDVAA LVEPLSVTWH AVKISGFKKG SSALVLGAGP
    210 220 230 240 250
    IGLCTILVLK GMGASKIVVS EIAERRIEMA KKLGVEVFNP SKHGHKSIEI
    260 270 280 290 300
    LRGLTKSHDG FDYSYDCSGI QVTFETSLKA LTFKGTATNI AVWGPKPVPF
    310 320 330 340 350
    QPMDVTLQEK VMTGSIGYVV EDFEEVVRAI HNGDIAMEDC KQLITGKQRI
    360 370 380
    EDGWEKGFQE LMDHKESNVK ILLTPNNHGE MK
    Length:382
    Mass (Da):41,582
    Last modified:July 27, 2011 - v2
    Checksum:i1776FA5968EC1A12
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti322 – 3221D → A in AAC04974 (PubMed:7731988).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U12980 Genomic DNA. Translation: AAC04974.1.
    AY692922 Genomic DNA. Translation: AAT92941.1.
    BK006935 Genomic DNA. Translation: DAA06929.2.
    PIRiS51962.
    RefSeqiNP_009341.2. NM_001178202.2.

    Genome annotation databases

    EnsemblFungiiYAL060W; YAL060W; YAL060W.
    GeneIDi851239.
    KEGGisce:YAL060W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U12980 Genomic DNA. Translation: AAC04974.1.
    AY692922 Genomic DNA. Translation: AAT92941.1.
    BK006935 Genomic DNA. Translation: DAA06929.2.
    PIRiS51962.
    RefSeqiNP_009341.2. NM_001178202.2.

    3D structure databases

    ProteinModelPortaliP39714.
    SMRiP39714. Positions 1-373.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi31770. 32 interactions.
    DIPiDIP-5356N.
    IntActiP39714. 2 interactions.
    MINTiMINT-487549.

    Proteomic databases

    MaxQBiP39714.
    PaxDbiP39714.
    PeptideAtlasiP39714.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYAL060W; YAL060W; YAL060W.
    GeneIDi851239.
    KEGGisce:YAL060W.

    Organism-specific databases

    CYGDiYAL060w.
    EuPathDBiFungiDB:YAL060W.
    SGDiS000000056. BDH1.

    Phylogenomic databases

    eggNOGiCOG1063.
    GeneTreeiENSGT00780000122413.
    HOGENOMiHOG000294670.
    InParanoidiP39714.
    KOiK00004.
    OMAiRARNHEN.
    OrthoDBiEOG7DVDNZ.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14023.
    YEAST:YAL060W-MONOMER.
    BRENDAi1.1.1.303. 984.
    1.1.1.4. 984.

    Miscellaneous databases

    NextBioi968165.
    PROiP39714.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.180.10. 1 hit.
    InterProiIPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR002085. ADH_SF_Zn-type.
    IPR002328. ADH_Zn_CS.
    IPR011032. GroES-like.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11695. PTHR11695. 1 hit.
    PfamiPF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF50129. SSF50129. 1 hit.
    PROSITEiPS00059. ADH_ZINC. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 322.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Characterization of a (2R,3R)-2,3-butanediol dehydrogenase as the Saccharomyces cerevisiae YAL060W gene product. Disruption and induction of the gene."
      Gonzalez E., Fernandez M.R., Larroy C., Sola L., Pericas M.A., Pares X., Biosca J.A.
      J. Biol. Chem. 275:35876-35885(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: ATCC 90845 / FY834.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-63, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBDH1_YEAST
    AccessioniPrimary (citable) accession number: P39714
    Secondary accession number(s): D6VPF9, E9P8Z3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 27, 2011
    Last modified: July 22, 2015
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 8730 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.