Reviewed, UniProtKB/Swiss-Prot P39714 (BDH1_YEAST)

Names and origin

Protein names

Recommended name:
    (R,R)-butanediol dehydrogenase
    EC=1.1.1.4

Gene names

Name:	BDH1
Synonyms:	BDH
Ordered Locus Names:	YAL060W
ORF Names:	FUN49

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(R,R)-butane-2,3-diol + NAD+ = (R)-acetoin + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Homodimer.
Miscellaneous	Present with 8730 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	butanediol biosynthetic process Inferred from mutant phenotype. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD
Molecular function	(R,R)-butanediol dehydrogenase activity Ref.2 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SER3	P40054	1	EBI-3501,EBI-16961

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	(R,R)-butanediol dehydrogenase		PRO_0000160788
Sites
	Metal binding	39	1	Zinc 1; catalytic By similarity
	Metal binding	73	1	Zinc 1; catalytic By similarity
	Metal binding	103	1	Zinc 2 By similarity
	Metal binding	120	1	Zinc 2 By similarity
	Metal binding	123	1	Zinc 2 By similarity
	Metal binding	131	1	Zinc 2 By similarity
	Metal binding	173	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39714-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 1006FA596DE91A17	FASTA	382	41,538
	10 20 30 40 50 60 MRALAYFKKG DIHFTNDIPR PEIQTDDEVI IDVSWCGICG SDLHEYLDGP IFMPKDGECH 70 80 90 100 110 120 KLSNAALPLA MGHEMSGIVS KVGPKVTKVK VGDHVVVDAA SSCADLHCWP HSKFYNSKPC 130 140 150 160 170 180 DACQRGSENL CTHAGFVGLG VISGGFAEQV VVSQHHIIPV PKEIPLDVAA LVEPLSVTWH 190 200 210 220 230 240 AVKISGFKKG SSALVLGAGP IGLCTILVLK GMGASKIVVS EIAERRIEMA KKLGVEVFNP 250 260 270 280 290 300 SKHGHKSIEI LRGLTKSHDG FDYSYDCSGI QVTFETSLKA LTFKGTATNI AVWGPKPVPF 310 320 330 340 350 360 QPMDVTLQEK VMTGSIGYVV EAFEEVVRAI HNGDIAMEDC KQLITGKQRI EDGWEKGFQE 370 380 LMDHKESNVK ILLTPNNHGE MK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Characterization of a (2R,3R)-2,3-butanediol dehydrogenase as the Saccharomyces cerevisiae YAL060W gene product. Disruption and induction of the gene." Gonzalez E., Fernandez M.R., Larroy C., Sola L., Pericas M.A., Pares X., Biosca J.A. J. Biol. Chem. 275:35876-35885(2000) [PubMed: 10938079] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 90845 / FY834.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12980 Genomic DNA. Translation: AAC04974.1.
PIR	S51962.
RefSeq	NP_009341.1.
3D structure databases
SMR	P39714. Positions 1-377.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5356N.
IntAct	P39714. 3 interactions.
STRING	P39714.
Proteomic databases
PeptideAtlas	P39714.
Genome annotation databases
Ensembl	YAL060W; YAL060W; YAL060W; Saccharomyces cerevisiae. [Genome view]
GeneID	851239.
KEGG	sce:YAL060W.
NMPDR	fig|4932.3.peg.15.
Organism-specific databases
CYGD	YAL060w.
SGD	S000000056. BDH1.
Phylogenomic databases
eggNOG	fuNOG07977.
HOGENOM	HBG753318.
OMA	ASVGYRH.
OrthoDB	EOG9RR80D.
PhylomeDB	P39714.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14023.
BRENDA	1.1.1.4. 250.
Gene expression databases
ArrayExpress	P39714.
Genevestigator	P39714.
GermOnline	YAL060W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	968165.

Entry information

Entry name

BDH1_YEAST

Accession

Primary (citable) accession number: P39714

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	February 9, 2010
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)