P39713 (BDH2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable diacetyl reductase [(R)-acetoin forming] 2
EC=1.1.1.303

Gene names

Name:	BDH2
Ordered Locus Names:	YAL061W
ORF Names:	FUN50

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the irreversible reduction of 2,3-butanediol to (S)-acetoin in the presence of NADH Potential.
Catalytic activity	(R)-acetoin + NAD+ = diacetyl + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subcellular location	Cytoplasm. Nucleus Ref.5.
Induction	Expression is controled by the PDR1 transcription factor and the glucose-responsive transcription factor RGT1. Ref.4 Ref.7
Miscellaneous	Present with 3090 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from direct assay Ref.5. Source: SGD nucleus Inferred from direct assay Ref.5. Source: SGD
Molecular function	diacetyl reductase ((R)-acetoin forming) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Probable diacetyl reductase [(R)-acetoin forming] 2		PRO_0000160897
Sites
	Metal binding	39	1	Zinc 1; catalytic By similarity
	Metal binding	64	1	Zinc 1; catalytic By similarity
	Metal binding	120	1	Zinc 2 By similarity
	Metal binding	123	1	Zinc 2 By similarity
	Metal binding	131	1	Zinc 2 By similarity
	Metal binding	173	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39713 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D921CEDBE61D0151	FASTA	417	46,099
	10 20 30 40 50 60 MRALAYFGKG NIRFTNHLKE PHIVAPDELV IDIEWCGICG TDLHEYTDGP IFFPEDGHTH 70 80 90 100 110 120 EISHNPLPQA MGHEMAGTVL EVGPGVKNLK VGDKVVVEPT GTCRDRYRWP LSPNVDKEWC 130 140 150 160 170 180 AACKKGYYNI CSYLGLCGAG VQSGGFAERV VMNESHCYKV PDFVPLDVAA LIQPLAVCWH 190 200 210 220 230 240 AIRVCEFKAG STALIIGAGP IGLGTILALN AAGCKDIVVS EPAKVRRELA EKMGARVYDP 250 260 270 280 290 300 TAHAAKESID YLRSIADGGD GFDYTFDCSG LEVTLNAAIQ CLTFRGTAVN LAMWGHHKIQ 310 320 330 340 350 360 FSPMDITLHE RKYTGSMCYT HHDFEAVIEA LEEGRIDIDR ARHMITGRVN IEDGLDGAIM 370 380 390 400 410 KLINEKESTI KIILTPNNHG ELNREADNEK KEISELSSRK DQERLRESIN EAKLRHT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Novel target genes of the yeast regulator Pdr1p: a contribution of the TPO1 gene in resistance to quinidine and other drugs." do Valle Matta M.A., Jonniaux J.-L., Balzi E., Goffeau A., van den Hazel B. Gene 272:111-119(2001) [PubMed: 11470516] [Abstract] Cited for: INDUCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Regulatory network connecting two glucose signal transduction pathways in Saccharomyces cerevisiae." Kaniak A., Xue Z., Macool D., Kim J.-H., Johnston M. Eukaryot. Cell 3:221-231(2004) [PubMed: 14871952] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12980 Genomic DNA. Translation: AAC04973.1. AY692730 Genomic DNA. Translation: AAT92749.1. BK006935 Genomic DNA. Translation: DAA06928.1.
PIR	S51961.
RefSeq	NP_009340.1. NM_001178203.1.
3D structure databases
ProteinModelPortal	P39713.
SMR	P39713. Positions 1-378.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6734N.
IntAct	P39713. 4 interactions.
MINT	MINT-641070.
STRING	P39713.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL061W; YAL061W; YAL061W.
GeneID	851238.
KEGG	sce:YAL061W.
NMPDR	fig|4932.3.peg.14.
Organism-specific databases
CYGD	YAL061w.
SGD	S000000057. BDH2.
Phylogenomic databases
eggNOG	fuNOG07977.
GeneTree	EFGT00050000001794.
HOGENOM	HBG753318.
OMA	SIWEREA.
OrthoDB	EOG4H49CR.
Gene expression databases
ArrayExpress	P39713.
Genevestigator	P39713.
GermOnline	YAL061W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00004.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968162.

Entry information

Entry name

BDH2_YEAST

Accession

Primary (citable) accession number: P39713
Secondary accession number(s): D6VPF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program