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Protein

Probable diacetyl reductase [(R)-acetoin forming] 2

Gene

BDH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible reduction of 2,3-butanediol to (S)-acetoin in the presence of NADH.Curated

Catalytic activityi

(R)-acetoin + NAD+ = diacetyl + NADH.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi39 – 391Zinc 1; catalyticBy similarity
Metal bindingi64 – 641Zinc 1; catalyticBy similarity
Metal bindingi120 – 1201Zinc 2By similarity
Metal bindingi123 – 1231Zinc 2By similarity
Metal bindingi131 – 1311Zinc 2By similarity
Metal bindingi173 – 1731Zinc 1; catalyticBy similarity

GO - Molecular functioni

  1. diacetyl reductase ((R)-acetoin forming) activity Source: UniProtKB-EC
  2. oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: SGD
  3. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-28863-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable diacetyl reductase [(R)-acetoin forming] 2 (EC:1.1.1.303)
Gene namesi
Name:BDH2
Ordered Locus Names:YAL061W
ORF Names:FUN50
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL061w.
SGDiS000000057. BDH2.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 417417Probable diacetyl reductase [(R)-acetoin forming] 2PRO_0000160897Add
BLAST

Proteomic databases

MaxQBiP39713.
PaxDbiP39713.

Expressioni

Inductioni

Expression is controlled by the PDR1 transcription factor and the glucose-responsive transcription factor RGT1.2 Publications

Gene expression databases

GenevestigatoriP39713.

Interactioni

Protein-protein interaction databases

BioGridi31769. 9 interactions.
DIPiDIP-6734N.
IntActiP39713. 2 interactions.
MINTiMINT-641070.
STRINGi4932.YAL061W.

Structurei

3D structure databases

ProteinModelPortaliP39713.
SMRiP39713. Positions 1-356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1063.
GeneTreeiENSGT00780000122413.
HOGENOMiHOG000294670.
InParanoidiP39713.
KOiK00004.
OMAiLAVCWHA.
OrthoDBiEOG7DVDNZ.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39713-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRALAYFGKG NIRFTNHLKE PHIVAPDELV IDIEWCGICG TDLHEYTDGP
60 70 80 90 100
IFFPEDGHTH EISHNPLPQA MGHEMAGTVL EVGPGVKNLK VGDKVVVEPT
110 120 130 140 150
GTCRDRYRWP LSPNVDKEWC AACKKGYYNI CSYLGLCGAG VQSGGFAERV
160 170 180 190 200
VMNESHCYKV PDFVPLDVAA LIQPLAVCWH AIRVCEFKAG STALIIGAGP
210 220 230 240 250
IGLGTILALN AAGCKDIVVS EPAKVRRELA EKMGARVYDP TAHAAKESID
260 270 280 290 300
YLRSIADGGD GFDYTFDCSG LEVTLNAAIQ CLTFRGTAVN LAMWGHHKIQ
310 320 330 340 350
FSPMDITLHE RKYTGSMCYT HHDFEAVIEA LEEGRIDIDR ARHMITGRVN
360 370 380 390 400
IEDGLDGAIM KLINEKESTI KIILTPNNHG ELNREADNEK KEISELSSRK
410
DQERLRESIN EAKLRHT
Length:417
Mass (Da):46,099
Last modified:February 1, 1995 - v1
Checksum:iD921CEDBE61D0151
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. Translation: AAC04973.1.
AY692730 Genomic DNA. Translation: AAT92749.1.
BK006935 Genomic DNA. Translation: DAA06928.1.
PIRiS51961.
RefSeqiNP_009340.1. NM_001178203.1.

Genome annotation databases

EnsemblFungiiYAL061W; YAL061W; YAL061W.
GeneIDi851238.
KEGGisce:YAL061W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. Translation: AAC04973.1.
AY692730 Genomic DNA. Translation: AAT92749.1.
BK006935 Genomic DNA. Translation: DAA06928.1.
PIRiS51961.
RefSeqiNP_009340.1. NM_001178203.1.

3D structure databases

ProteinModelPortaliP39713.
SMRiP39713. Positions 1-356.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31769. 9 interactions.
DIPiDIP-6734N.
IntActiP39713. 2 interactions.
MINTiMINT-641070.
STRINGi4932.YAL061W.

Proteomic databases

MaxQBiP39713.
PaxDbiP39713.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL061W; YAL061W; YAL061W.
GeneIDi851238.
KEGGisce:YAL061W.

Organism-specific databases

CYGDiYAL061w.
SGDiS000000057. BDH2.

Phylogenomic databases

eggNOGiCOG1063.
GeneTreeiENSGT00780000122413.
HOGENOMiHOG000294670.
InParanoidiP39713.
KOiK00004.
OMAiLAVCWHA.
OrthoDBiEOG7DVDNZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-28863-MONOMER.

Miscellaneous databases

NextBioi968162.

Gene expression databases

GenevestigatoriP39713.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
PROSITEiPS00059. ADH_ZINC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Novel target genes of the yeast regulator Pdr1p: a contribution of the TPO1 gene in resistance to quinidine and other drugs."
    do Valle Matta M.A., Jonniaux J.-L., Balzi E., Goffeau A., van den Hazel B.
    Gene 272:111-119(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Regulatory network connecting two glucose signal transduction pathways in Saccharomyces cerevisiae."
    Kaniak A., Xue Z., Macool D., Kim J.-H., Johnston M.
    Eukaryot. Cell 3:221-231(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.

Entry informationi

Entry nameiBDH2_YEAST
AccessioniPrimary (citable) accession number: P39713
Secondary accession number(s): D6VPF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: February 4, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3090 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.