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P39712

- FLO9_YEAST

UniProt

P39712 - FLO9_YEAST

Protein

Flocculation protein FLO9

Gene

FLO9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells.

    GO - Molecular functioni

    1. mannose binding Source: SGD

    GO - Biological processi

    1. cell-substrate adhesion Source: SGD
    2. flocculation Source: SGD
    3. flocculation via cell wall protein-carbohydrate interaction Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28864-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Flocculation protein FLO9
    Short name:
    Flocculin-9
    Gene namesi
    Name:FLO9
    Ordered Locus Names:YAL063C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAL063c.
    SGDiS000000059. FLO9.

    Subcellular locationi

    Secretedcell wall 1 Publication. Membrane Curated; Lipid-anchorGPI-anchor Curated
    Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP).

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular region Source: UniProtKB-KW
    3. fungal-type cell wall Source: SGD

    Keywords - Cellular componenti

    Cell wall, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 12991275Flocculation protein FLO9PRO_0000021277Add
    BLAST
    Propeptidei1300 – 132223Removed in mature formSequence AnalysisPRO_0000372454Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi203 – 2031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi257 – 2571N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi262 – 2621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi270 – 2701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi419 – 4191N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi464 – 4641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi509 – 5091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi554 – 5541N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi599 – 5991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi644 – 6441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi689 – 6891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi734 – 7341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi888 – 8881N-linked (GlcNAc...)Sequence Analysis
    Lipidationi1299 – 12991GPI-anchor amidated glycineSequence Analysis

    Post-translational modificationi

    The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.By similarity

    Keywords - PTMi

    Glycoprotein, GPI-anchor, Lipoprotein

    Proteomic databases

    PaxDbiP39712.

    Expressioni

    Gene expression databases

    GenevestigatoriP39712.

    Interactioni

    Protein-protein interaction databases

    BioGridi31767. 14 interactions.
    IntActiP39712. 1 interaction.
    STRINGi4932.YAL063C.

    Structurei

    3D structure databases

    ProteinModelPortaliP39712.
    SMRiP39712. Positions 22-269.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati278 – 322451-1Add
    BLAST
    Repeati323 – 367451-2Add
    BLAST
    Repeati368 – 412451-3Add
    BLAST
    Repeati413 – 457451-4Add
    BLAST
    Repeati458 – 502451-5Add
    BLAST
    Repeati503 – 547451-6Add
    BLAST
    Repeati548 – 592451-7Add
    BLAST
    Repeati593 – 637451-8Add
    BLAST
    Repeati638 – 682451-9Add
    BLAST
    Repeati683 – 727451-10Add
    BLAST
    Repeati728 – 772451-11Add
    BLAST
    Repeati773 – 817451-12Add
    BLAST
    Repeati818 – 862451-13Add
    BLAST
    Repeati892 – 906152-1Add
    BLAST
    Repeati907 – 921152-2Add
    BLAST
    Repeati922 – 936152-3Add
    BLAST
    Repeati1013 – 1063513-1Add
    BLAST
    Repeati1085 – 1135513-2Add
    BLAST
    Repeati1136 – 1186513-3Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni197 – 24044Sugar recognitionBy similarityAdd
    BLAST
    Regioni278 – 86258513 X 45 AA approximate tandem repeats, Thr-richAdd
    BLAST
    Regioni892 – 936453 X 15 AA approximate repeats, Ser-richAdd
    BLAST
    Regioni1013 – 11861743 X 51 AA approximate repeats, Thr-richAdd
    BLAST

    Domaini

    The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells By similarity. The Ser/Thr-rich repeats are also important for proper cell wall targeting of the protein.By similarity

    Sequence similaritiesi

    Belongs to the flocculin family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00660000095872.
    HOGENOMiHOG000112611.
    OMAiTIETIYY.
    OrthoDBiEOG7H4F6F.

    Family and domain databases

    Gene3Di3.90.182.10. 1 hit.
    InterProiIPR001389. Flocculin.
    IPR025928. Flocculin_t3_rpt.
    IPR011658. PA14.
    [Graphical view]
    PfamiPF00624. Flocculin. 13 hits.
    PF13928. Flocculin_t3. 3 hits.
    PF07691. PA14. 1 hit.
    [Graphical view]
    SMARTiSM00758. PA14. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P39712-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLAHYCLLL AIVTLLGLTN VVSATTAACL PANSRKNGMN VNFYQYSLRD     50
    SSTYSNAAYM AYGYASKTKL GSVGGQTDIS IDYNIPCVSS SGTFPCPQED 100
    LYGNWGCKGI GACSNNPIIA YWSTDLFGFY TTPTNVTLEM TGYFLPPQTG 150
    SYTFKFATVD DSAILSVGGS IAFECCAQEQ PPITSTNFTI NGIKPWNGSP 200
    PDNITGTVYM YAGFYYPMKI VYSNAVAWGT LPISVTLPDG TTVSDDFEGY 250
    VYTFDNNLSQ PNCTIPDPSN YTVSTTITTT EPWTGTFTST STEMTTVTGT 300
    NGVPTDETVI VIRTPTTAST IITTTEPWNS TFTSTSTELT TVTGTNGVRT 350
    DETIIVIRTP TTATTAITTT EPWNSTFTST STELTTVTGT NGLPTDETII 400
    VIRTPTTATT AMTTTQPWND TFTSTSTELT TVTGTNGLPT DETIIVIRTP 450
    TTATTAMTTT QPWNDTFTST STELTTVTGT NGLPTDETII VIRTPTTATT 500
    AMTTTQPWND TFTSTSTEIT TVTGTNGLPT DETIIVIRTP TTATTAMTTT 550
    QPWNDTFTST STEMTTVTGT NGLPTDETII VIRTPTTATT AITTTEPWNS 600
    TFTSTSTEMT TVTGTNGLPT DETIIVIRTP TTATTAITTT QPWNDTFTST 650
    STEMTTVTGT NGLPTDETII VIRTPTTATT AMTTTQPWND TFTSTSTEIT 700
    TVTGTNGLPT DETIIVIRTP TTATTAMTTT QPWNDTFTST STEMTTVTGT 750
    NGVPTDETVI VIRTPTSEGL ISTTTEPWTG TFTSTSTEMT TVTGTNGQPT 800
    DETVIVIRTP TSEGLVTTTT EPWTGTFTST STEMTTITGT NGQPTDETVI 850
    IVKTPTTAIS SSLSSSSGQI TSFITSARPI ITPFYPSNGT SVISSSVISS 900
    SDTSSLVISS SVTSSLVTSS PVISSSFISS PVISSTTTSA SILSESSKSS 950
    VIPTSSSTSG SSESETGSAS SASSSSSISS ESPKSTYSSS SLPPVTSATT 1000
    SQEITSSLPP VTTTKTSEQT TLVTVTSCES HVCTESISSA IVSTATVTVS 1050
    GATTEYTTWC PISTTEITKQ TTETTKQTKG TTEQTTETTK QTTVVTISSC 1100
    ESDVCSKTAS PAIVSTSTAT INGVTTEYTT WCPISTTESK QQTTLVTVTS 1150
    CGSGVCSETT SPAIVSTATA TVNDVVTVYS TWRPQTTNEQ SVSSKMNSAT 1200
    SETTTNTGAA ETTTSTGAAE TKTVVTSSIS RFNHAETQTA SATDVIGHSS 1250
    SVVSVSETGN TKSLTSSGLS TMSQQPRSTP ASSMVGSSTA SLEISTYAGS 1300
    ANSLLAGSGL SVFIASLLLA II 1322
    Length:1,322
    Mass (Da):138,073
    Last modified:February 1, 1996 - v2
    Checksum:iAADFD1FF13267CEA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12980 Genomic DNA. Translation: AAC04971.1.
    BK006935 Genomic DNA. Translation: DAA06926.1.
    PIRiS51959.
    RefSeqiNP_009338.1. NM_001178205.1.

    Genome annotation databases

    EnsemblFungiiYAL063C; YAL063C; YAL063C.
    GeneIDi851236.
    KEGGisce:YAL063C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U12980 Genomic DNA. Translation: AAC04971.1 .
    BK006935 Genomic DNA. Translation: DAA06926.1 .
    PIRi S51959.
    RefSeqi NP_009338.1. NM_001178205.1.

    3D structure databases

    ProteinModelPortali P39712.
    SMRi P39712. Positions 22-269.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31767. 14 interactions.
    IntActi P39712. 1 interaction.
    STRINGi 4932.YAL063C.

    Proteomic databases

    PaxDbi P39712.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL063C ; YAL063C ; YAL063C .
    GeneIDi 851236.
    KEGGi sce:YAL063C.

    Organism-specific databases

    CYGDi YAL063c.
    SGDi S000000059. FLO9.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00660000095872.
    HOGENOMi HOG000112611.
    OMAi TIETIYY.
    OrthoDBi EOG7H4F6F.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28864-MONOMER.

    Miscellaneous databases

    NextBioi 968156.

    Gene expression databases

    Genevestigatori P39712.

    Family and domain databases

    Gene3Di 3.90.182.10. 1 hit.
    InterProi IPR001389. Flocculin.
    IPR025928. Flocculin_t3_rpt.
    IPR011658. PA14.
    [Graphical view ]
    Pfami PF00624. Flocculin. 13 hits.
    PF13928. Flocculin_t3. 3 hits.
    PF07691. PA14. 1 hit.
    [Graphical view ]
    SMARTi SM00758. PA14. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family."
      Teunissen A.W.R.H., Steensma H.Y.
      Yeast 11:1001-1013(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    4. "Multiple sequence signals determine the distribution of glycosylphosphatidylinositol proteins between the plasma membrane and cell wall in Saccharomyces cerevisiae."
      Frieman M.B., Cormack B.P.
      Microbiology 150:3105-3114(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, REPEATS.
    5. "Intragenic tandem repeats generate functional variability."
      Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
      Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: REPEATS.

    Entry informationi

    Entry nameiFLO9_YEAST
    AccessioniPrimary (citable) accession number: P39712
    Secondary accession number(s): D6VPF6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3