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P39712 (FLO9_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Flocculation protein FLO9

Short name=Flocculin-9
Gene names
Name:FLO9
Ordered Locus Names:YAL063C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1322 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells.

Subcellular location

Secretedcell wall Probable. Membrane; Lipid-anchorGPI-anchor Potential. Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP). Ref.4

Domain

The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells By similarity. The Ser/Thr-rich repeats are also important for proper cell wall targeting of the protein.

Post-translational modification

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.

Sequence similarities

Belongs to the flocculin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 12991275Flocculation protein FLO9
PRO_0000021277
Propeptide1300 – 132223Removed in mature form Potential
PRO_0000372454

Regions

Repeat278 – 322451-1
Repeat323 – 367451-2
Repeat368 – 412451-3
Repeat413 – 457451-4
Repeat458 – 502451-5
Repeat503 – 547451-6
Repeat548 – 592451-7
Repeat593 – 637451-8
Repeat638 – 682451-9
Repeat683 – 727451-10
Repeat728 – 772451-11
Repeat773 – 817451-12
Repeat818 – 862451-13
Repeat892 – 906152-1
Repeat907 – 921152-2
Repeat922 – 936152-3
Repeat1013 – 1063513-1
Repeat1085 – 1135513-2
Repeat1136 – 1186513-3
Region197 – 24044Sugar recognition By similarity
Region278 – 86258513 X 45 AA approximate tandem repeats, Thr-rich
Region892 – 936453 X 15 AA approximate repeats, Ser-rich
Region1013 – 11861743 X 51 AA approximate repeats, Thr-rich

Amino acid modifications

Lipidation12991GPI-anchor amidated glycine Potential
Glycosylation1351N-linked (GlcNAc...) Potential
Glycosylation1871N-linked (GlcNAc...) Potential
Glycosylation2031N-linked (GlcNAc...) Potential
Glycosylation2571N-linked (GlcNAc...) Potential
Glycosylation2621N-linked (GlcNAc...) Potential
Glycosylation2701N-linked (GlcNAc...) Potential
Glycosylation3291N-linked (GlcNAc...) Potential
Glycosylation4191N-linked (GlcNAc...) Potential
Glycosylation4641N-linked (GlcNAc...) Potential
Glycosylation5091N-linked (GlcNAc...) Potential
Glycosylation5541N-linked (GlcNAc...) Potential
Glycosylation5991N-linked (GlcNAc...) Potential
Glycosylation6441N-linked (GlcNAc...) Potential
Glycosylation6891N-linked (GlcNAc...) Potential
Glycosylation7341N-linked (GlcNAc...) Potential
Glycosylation8881N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P39712 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: AADFD1FF13267CEA

FASTA1,322138,073
        10         20         30         40         50         60 
MSLAHYCLLL AIVTLLGLTN VVSATTAACL PANSRKNGMN VNFYQYSLRD SSTYSNAAYM 

        70         80         90        100        110        120 
AYGYASKTKL GSVGGQTDIS IDYNIPCVSS SGTFPCPQED LYGNWGCKGI GACSNNPIIA 

       130        140        150        160        170        180 
YWSTDLFGFY TTPTNVTLEM TGYFLPPQTG SYTFKFATVD DSAILSVGGS IAFECCAQEQ 

       190        200        210        220        230        240 
PPITSTNFTI NGIKPWNGSP PDNITGTVYM YAGFYYPMKI VYSNAVAWGT LPISVTLPDG 

       250        260        270        280        290        300 
TTVSDDFEGY VYTFDNNLSQ PNCTIPDPSN YTVSTTITTT EPWTGTFTST STEMTTVTGT 

       310        320        330        340        350        360 
NGVPTDETVI VIRTPTTAST IITTTEPWNS TFTSTSTELT TVTGTNGVRT DETIIVIRTP 

       370        380        390        400        410        420 
TTATTAITTT EPWNSTFTST STELTTVTGT NGLPTDETII VIRTPTTATT AMTTTQPWND 

       430        440        450        460        470        480 
TFTSTSTELT TVTGTNGLPT DETIIVIRTP TTATTAMTTT QPWNDTFTST STELTTVTGT 

       490        500        510        520        530        540 
NGLPTDETII VIRTPTTATT AMTTTQPWND TFTSTSTEIT TVTGTNGLPT DETIIVIRTP 

       550        560        570        580        590        600 
TTATTAMTTT QPWNDTFTST STEMTTVTGT NGLPTDETII VIRTPTTATT AITTTEPWNS 

       610        620        630        640        650        660 
TFTSTSTEMT TVTGTNGLPT DETIIVIRTP TTATTAITTT QPWNDTFTST STEMTTVTGT 

       670        680        690        700        710        720 
NGLPTDETII VIRTPTTATT AMTTTQPWND TFTSTSTEIT TVTGTNGLPT DETIIVIRTP 

       730        740        750        760        770        780 
TTATTAMTTT QPWNDTFTST STEMTTVTGT NGVPTDETVI VIRTPTSEGL ISTTTEPWTG 

       790        800        810        820        830        840 
TFTSTSTEMT TVTGTNGQPT DETVIVIRTP TSEGLVTTTT EPWTGTFTST STEMTTITGT 

       850        860        870        880        890        900 
NGQPTDETVI IVKTPTTAIS SSLSSSSGQI TSFITSARPI ITPFYPSNGT SVISSSVISS 

       910        920        930        940        950        960 
SDTSSLVISS SVTSSLVTSS PVISSSFISS PVISSTTTSA SILSESSKSS VIPTSSSTSG 

       970        980        990       1000       1010       1020 
SSESETGSAS SASSSSSISS ESPKSTYSSS SLPPVTSATT SQEITSSLPP VTTTKTSEQT 

      1030       1040       1050       1060       1070       1080 
TLVTVTSCES HVCTESISSA IVSTATVTVS GATTEYTTWC PISTTEITKQ TTETTKQTKG 

      1090       1100       1110       1120       1130       1140 
TTEQTTETTK QTTVVTISSC ESDVCSKTAS PAIVSTSTAT INGVTTEYTT WCPISTTESK 

      1150       1160       1170       1180       1190       1200 
QQTTLVTVTS CGSGVCSETT SPAIVSTATA TVNDVVTVYS TWRPQTTNEQ SVSSKMNSAT 

      1210       1220       1230       1240       1250       1260 
SETTTNTGAA ETTTSTGAAE TKTVVTSSIS RFNHAETQTA SATDVIGHSS SVVSVSETGN 

      1270       1280       1290       1300       1310       1320 
TKSLTSSGLS TMSQQPRSTP ASSMVGSSTA SLEISTYAGS ANSLLAGSGL SVFIASLLLA 


II 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family."
Teunissen A.W.R.H., Steensma H.Y.
Yeast 11:1001-1013(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[4]"Multiple sequence signals determine the distribution of glycosylphosphatidylinositol proteins between the plasma membrane and cell wall in Saccharomyces cerevisiae."
Frieman M.B., Cormack B.P.
Microbiology 150:3105-3114(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, REPEATS.
[5]"Intragenic tandem repeats generate functional variability."
Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: REPEATS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U12980 Genomic DNA. Translation: AAC04971.1.
BK006935 Genomic DNA. Translation: DAA06926.1.
PIRS51959.
RefSeqNP_009338.1. NM_001178205.1.

3D structure databases

ProteinModelPortalP39712.
SMRP39712. Positions 22-269.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31767. 14 interactions.
IntActP39712. 1 interaction.
STRING4932.YAL063C.

Proteomic databases

PaxDbP39712.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAL063C; YAL063C; YAL063C.
GeneID851236.
KEGGsce:YAL063C.

Organism-specific databases

CYGDYAL063c.
SGDS000000059. FLO9.

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00660000095872.
HOGENOMHOG000112611.
OMATIETIYY.
OrthoDBEOG7H4F6F.

Enzyme and pathway databases

BioCycYEAST:G3O-28864-MONOMER.

Gene expression databases

GenevestigatorP39712.

Family and domain databases

Gene3D3.90.182.10. 1 hit.
InterProIPR001389. Flocculin.
IPR025928. Flocculin_t3_rpt.
IPR011658. PA14.
[Graphical view]
PfamPF00624. Flocculin. 13 hits.
PF13928. Flocculin_t3. 3 hits.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTSM00758. PA14. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968156.

Entry information

Entry nameFLO9_YEAST
AccessionPrimary (citable) accession number: P39712
Secondary accession number(s): D6VPF6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families