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P39712

- FLO9_YEAST

UniProt

P39712 - FLO9_YEAST

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Protein

Flocculation protein FLO9

Gene
FLO9, YAL063C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells.

GO - Molecular functioni

  1. mannose binding Source: SGD

GO - Biological processi

  1. cell-substrate adhesion Source: SGD
  2. flocculation Source: SGD
  3. flocculation via cell wall protein-carbohydrate interaction Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-28864-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flocculation protein FLO9
Short name:
Flocculin-9
Gene namesi
Name:FLO9
Ordered Locus Names:YAL063C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL063c.
SGDiS000000059. FLO9.

Subcellular locationi

Secretedcell wall Inferred. Membrane; Lipid-anchorGPI-anchor Reviewed prediction
Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP).1 Publication

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
  3. fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed predictionAdd
BLAST
Chaini25 – 12991275Flocculation protein FLO9PRO_0000021277Add
BLAST
Propeptidei1300 – 132223Removed in mature form Reviewed predictionPRO_0000372454Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi135 – 1351N-linked (GlcNAc...) Reviewed prediction
Glycosylationi187 – 1871N-linked (GlcNAc...) Reviewed prediction
Glycosylationi203 – 2031N-linked (GlcNAc...) Reviewed prediction
Glycosylationi257 – 2571N-linked (GlcNAc...) Reviewed prediction
Glycosylationi262 – 2621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi270 – 2701N-linked (GlcNAc...) Reviewed prediction
Glycosylationi329 – 3291N-linked (GlcNAc...) Reviewed prediction
Glycosylationi419 – 4191N-linked (GlcNAc...) Reviewed prediction
Glycosylationi464 – 4641N-linked (GlcNAc...) Reviewed prediction
Glycosylationi509 – 5091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi554 – 5541N-linked (GlcNAc...) Reviewed prediction
Glycosylationi599 – 5991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi644 – 6441N-linked (GlcNAc...) Reviewed prediction
Glycosylationi689 – 6891N-linked (GlcNAc...) Reviewed prediction
Glycosylationi734 – 7341N-linked (GlcNAc...) Reviewed prediction
Glycosylationi888 – 8881N-linked (GlcNAc...) Reviewed prediction
Lipidationi1299 – 12991GPI-anchor amidated glycine Reviewed prediction

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP39712.

Expressioni

Gene expression databases

GenevestigatoriP39712.

Interactioni

Protein-protein interaction databases

BioGridi31767. 14 interactions.
IntActiP39712. 1 interaction.
STRINGi4932.YAL063C.

Structurei

3D structure databases

ProteinModelPortaliP39712.
SMRiP39712. Positions 22-269.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati278 – 322451-1Add
BLAST
Repeati323 – 367451-2Add
BLAST
Repeati368 – 412451-3Add
BLAST
Repeati413 – 457451-4Add
BLAST
Repeati458 – 502451-5Add
BLAST
Repeati503 – 547451-6Add
BLAST
Repeati548 – 592451-7Add
BLAST
Repeati593 – 637451-8Add
BLAST
Repeati638 – 682451-9Add
BLAST
Repeati683 – 727451-10Add
BLAST
Repeati728 – 772451-11Add
BLAST
Repeati773 – 817451-12Add
BLAST
Repeati818 – 862451-13Add
BLAST
Repeati892 – 906152-1Add
BLAST
Repeati907 – 921152-2Add
BLAST
Repeati922 – 936152-3Add
BLAST
Repeati1013 – 1063513-1Add
BLAST
Repeati1085 – 1135513-2Add
BLAST
Repeati1136 – 1186513-3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 24044Sugar recognition By similarityAdd
BLAST
Regioni278 – 86258513 X 45 AA approximate tandem repeats, Thr-richAdd
BLAST
Regioni892 – 936453 X 15 AA approximate repeats, Ser-richAdd
BLAST
Regioni1013 – 11861743 X 51 AA approximate repeats, Thr-richAdd
BLAST

Domaini

The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells By similarity. The Ser/Thr-rich repeats are also important for proper cell wall targeting of the protein.

Sequence similaritiesi

Belongs to the flocculin family.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00660000095872.
HOGENOMiHOG000112611.
OMAiTIETIYY.
OrthoDBiEOG7H4F6F.

Family and domain databases

Gene3Di3.90.182.10. 1 hit.
InterProiIPR001389. Flocculin.
IPR025928. Flocculin_t3_rpt.
IPR011658. PA14.
[Graphical view]
PfamiPF00624. Flocculin. 13 hits.
PF13928. Flocculin_t3. 3 hits.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTiSM00758. PA14. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39712-1 [UniParc]FASTAAdd to Basket

« Hide

MSLAHYCLLL AIVTLLGLTN VVSATTAACL PANSRKNGMN VNFYQYSLRD     50
SSTYSNAAYM AYGYASKTKL GSVGGQTDIS IDYNIPCVSS SGTFPCPQED 100
LYGNWGCKGI GACSNNPIIA YWSTDLFGFY TTPTNVTLEM TGYFLPPQTG 150
SYTFKFATVD DSAILSVGGS IAFECCAQEQ PPITSTNFTI NGIKPWNGSP 200
PDNITGTVYM YAGFYYPMKI VYSNAVAWGT LPISVTLPDG TTVSDDFEGY 250
VYTFDNNLSQ PNCTIPDPSN YTVSTTITTT EPWTGTFTST STEMTTVTGT 300
NGVPTDETVI VIRTPTTAST IITTTEPWNS TFTSTSTELT TVTGTNGVRT 350
DETIIVIRTP TTATTAITTT EPWNSTFTST STELTTVTGT NGLPTDETII 400
VIRTPTTATT AMTTTQPWND TFTSTSTELT TVTGTNGLPT DETIIVIRTP 450
TTATTAMTTT QPWNDTFTST STELTTVTGT NGLPTDETII VIRTPTTATT 500
AMTTTQPWND TFTSTSTEIT TVTGTNGLPT DETIIVIRTP TTATTAMTTT 550
QPWNDTFTST STEMTTVTGT NGLPTDETII VIRTPTTATT AITTTEPWNS 600
TFTSTSTEMT TVTGTNGLPT DETIIVIRTP TTATTAITTT QPWNDTFTST 650
STEMTTVTGT NGLPTDETII VIRTPTTATT AMTTTQPWND TFTSTSTEIT 700
TVTGTNGLPT DETIIVIRTP TTATTAMTTT QPWNDTFTST STEMTTVTGT 750
NGVPTDETVI VIRTPTSEGL ISTTTEPWTG TFTSTSTEMT TVTGTNGQPT 800
DETVIVIRTP TSEGLVTTTT EPWTGTFTST STEMTTITGT NGQPTDETVI 850
IVKTPTTAIS SSLSSSSGQI TSFITSARPI ITPFYPSNGT SVISSSVISS 900
SDTSSLVISS SVTSSLVTSS PVISSSFISS PVISSTTTSA SILSESSKSS 950
VIPTSSSTSG SSESETGSAS SASSSSSISS ESPKSTYSSS SLPPVTSATT 1000
SQEITSSLPP VTTTKTSEQT TLVTVTSCES HVCTESISSA IVSTATVTVS 1050
GATTEYTTWC PISTTEITKQ TTETTKQTKG TTEQTTETTK QTTVVTISSC 1100
ESDVCSKTAS PAIVSTSTAT INGVTTEYTT WCPISTTESK QQTTLVTVTS 1150
CGSGVCSETT SPAIVSTATA TVNDVVTVYS TWRPQTTNEQ SVSSKMNSAT 1200
SETTTNTGAA ETTTSTGAAE TKTVVTSSIS RFNHAETQTA SATDVIGHSS 1250
SVVSVSETGN TKSLTSSGLS TMSQQPRSTP ASSMVGSSTA SLEISTYAGS 1300
ANSLLAGSGL SVFIASLLLA II 1322
Length:1,322
Mass (Da):138,073
Last modified:February 1, 1996 - v2
Checksum:iAADFD1FF13267CEA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12980 Genomic DNA. Translation: AAC04971.1.
BK006935 Genomic DNA. Translation: DAA06926.1.
PIRiS51959.
RefSeqiNP_009338.1. NM_001178205.1.

Genome annotation databases

EnsemblFungiiYAL063C; YAL063C; YAL063C.
GeneIDi851236.
KEGGisce:YAL063C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12980 Genomic DNA. Translation: AAC04971.1 .
BK006935 Genomic DNA. Translation: DAA06926.1 .
PIRi S51959.
RefSeqi NP_009338.1. NM_001178205.1.

3D structure databases

ProteinModelPortali P39712.
SMRi P39712. Positions 22-269.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31767. 14 interactions.
IntActi P39712. 1 interaction.
STRINGi 4932.YAL063C.

Proteomic databases

PaxDbi P39712.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL063C ; YAL063C ; YAL063C .
GeneIDi 851236.
KEGGi sce:YAL063C.

Organism-specific databases

CYGDi YAL063c.
SGDi S000000059. FLO9.

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00660000095872.
HOGENOMi HOG000112611.
OMAi TIETIYY.
OrthoDBi EOG7H4F6F.

Enzyme and pathway databases

BioCyci YEAST:G3O-28864-MONOMER.

Miscellaneous databases

NextBioi 968156.

Gene expression databases

Genevestigatori P39712.

Family and domain databases

Gene3Di 3.90.182.10. 1 hit.
InterProi IPR001389. Flocculin.
IPR025928. Flocculin_t3_rpt.
IPR011658. PA14.
[Graphical view ]
Pfami PF00624. Flocculin. 13 hits.
PF13928. Flocculin_t3. 3 hits.
PF07691. PA14. 1 hit.
[Graphical view ]
SMARTi SM00758. PA14. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family."
    Teunissen A.W.R.H., Steensma H.Y.
    Yeast 11:1001-1013(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  4. "Multiple sequence signals determine the distribution of glycosylphosphatidylinositol proteins between the plasma membrane and cell wall in Saccharomyces cerevisiae."
    Frieman M.B., Cormack B.P.
    Microbiology 150:3105-3114(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, REPEATS.
  5. "Intragenic tandem repeats generate functional variability."
    Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
    Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REPEATS.

Entry informationi

Entry nameiFLO9_YEAST
AccessioniPrimary (citable) accession number: P39712
Secondary accession number(s): D6VPF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

Similar proteinsi