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P39712

- FLO9_YEAST

UniProt

P39712 - FLO9_YEAST

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Protein

Flocculation protein FLO9

Gene

FLO9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Cell wall protein that participates directly in adhesive cell-cell interactions during yeast flocculation, a reversible, asexual and Ca2+-dependent process in which cells adhere to form aggregates (flocs) consisting of thousands of cells. The lectin-like protein sticks out of the cell wall of flocculent cells and selectively binds mannose residues in the cell walls of adjacent cells.

GO - Molecular functioni

  1. mannose binding Source: SGD

GO - Biological processi

  1. cell-substrate adhesion Source: SGD
  2. flocculation Source: SGD
  3. flocculation via cell wall protein-carbohydrate interaction Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-28864-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flocculation protein FLO9
Short name:
Flocculin-9
Gene namesi
Name:FLO9
Ordered Locus Names:YAL063C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL063c.
SGDiS000000059. FLO9.

Subcellular locationi

Secretedcell wall 1 Publication. Membrane Curated; Lipid-anchorGPI-anchor Curated
Note: Covalently-linked GPI-modified cell wall protein (GPI-CWP).

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. extracellular region Source: UniProtKB-KW
  3. fungal-type cell wall Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 12991275Flocculation protein FLO9PRO_0000021277Add
BLAST
Propeptidei1300 – 132223Removed in mature formSequence AnalysisPRO_0000372454Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi187 – 1871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi203 – 2031N-linked (GlcNAc...)Sequence Analysis
Glycosylationi257 – 2571N-linked (GlcNAc...)Sequence Analysis
Glycosylationi262 – 2621N-linked (GlcNAc...)Sequence Analysis
Glycosylationi270 – 2701N-linked (GlcNAc...)Sequence Analysis
Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence Analysis
Glycosylationi419 – 4191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi464 – 4641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi509 – 5091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi554 – 5541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi599 – 5991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi644 – 6441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi689 – 6891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi734 – 7341N-linked (GlcNAc...)Sequence Analysis
Glycosylationi888 – 8881N-linked (GlcNAc...)Sequence Analysis
Lipidationi1299 – 12991GPI-anchor amidated glycineSequence Analysis

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By similarity).By similarity

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP39712.

Expressioni

Gene expression databases

GenevestigatoriP39712.

Interactioni

Protein-protein interaction databases

BioGridi31767. 14 interactions.
IntActiP39712. 1 interaction.
STRINGi4932.YAL063C.

Structurei

3D structure databases

ProteinModelPortaliP39712.
SMRiP39712. Positions 22-269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati278 – 322451-1Add
BLAST
Repeati323 – 367451-2Add
BLAST
Repeati368 – 412451-3Add
BLAST
Repeati413 – 457451-4Add
BLAST
Repeati458 – 502451-5Add
BLAST
Repeati503 – 547451-6Add
BLAST
Repeati548 – 592451-7Add
BLAST
Repeati593 – 637451-8Add
BLAST
Repeati638 – 682451-9Add
BLAST
Repeati683 – 727451-10Add
BLAST
Repeati728 – 772451-11Add
BLAST
Repeati773 – 817451-12Add
BLAST
Repeati818 – 862451-13Add
BLAST
Repeati892 – 906152-1Add
BLAST
Repeati907 – 921152-2Add
BLAST
Repeati922 – 936152-3Add
BLAST
Repeati1013 – 1063513-1Add
BLAST
Repeati1085 – 1135513-2Add
BLAST
Repeati1136 – 1186513-3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 24044Sugar recognitionBy similarityAdd
BLAST
Regioni278 – 86258513 X 45 AA approximate tandem repeats, Thr-richAdd
BLAST
Regioni892 – 936453 X 15 AA approximate repeats, Ser-richAdd
BLAST
Regioni1013 – 11861743 X 51 AA approximate repeats, Thr-richAdd
BLAST

Domaini

The number of the intragenic tandem repeats varies between different S.cerevisiae strains. There is a linear correlation between protein size and the extend of adhesion: the more repeats, the stronger the adhesion properties and the greater the fraction of flocculating cells (By similarity). The Ser/Thr-rich repeats are also important for proper cell wall targeting of the protein.By similarity

Sequence similaritiesi

Belongs to the flocculin family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00660000095872.
HOGENOMiHOG000112611.
InParanoidiP39712.
OMAiTIETIYY.
OrthoDBiEOG7H4F6F.

Family and domain databases

Gene3Di3.90.182.10. 1 hit.
InterProiIPR001389. Flocculin.
IPR025928. Flocculin_t3_rpt.
IPR011658. PA14.
[Graphical view]
PfamiPF00624. Flocculin. 13 hits.
PF13928. Flocculin_t3. 3 hits.
PF07691. PA14. 1 hit.
[Graphical view]
SMARTiSM00758. PA14. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39712 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLAHYCLLL AIVTLLGLTN VVSATTAACL PANSRKNGMN VNFYQYSLRD
60 70 80 90 100
SSTYSNAAYM AYGYASKTKL GSVGGQTDIS IDYNIPCVSS SGTFPCPQED
110 120 130 140 150
LYGNWGCKGI GACSNNPIIA YWSTDLFGFY TTPTNVTLEM TGYFLPPQTG
160 170 180 190 200
SYTFKFATVD DSAILSVGGS IAFECCAQEQ PPITSTNFTI NGIKPWNGSP
210 220 230 240 250
PDNITGTVYM YAGFYYPMKI VYSNAVAWGT LPISVTLPDG TTVSDDFEGY
260 270 280 290 300
VYTFDNNLSQ PNCTIPDPSN YTVSTTITTT EPWTGTFTST STEMTTVTGT
310 320 330 340 350
NGVPTDETVI VIRTPTTAST IITTTEPWNS TFTSTSTELT TVTGTNGVRT
360 370 380 390 400
DETIIVIRTP TTATTAITTT EPWNSTFTST STELTTVTGT NGLPTDETII
410 420 430 440 450
VIRTPTTATT AMTTTQPWND TFTSTSTELT TVTGTNGLPT DETIIVIRTP
460 470 480 490 500
TTATTAMTTT QPWNDTFTST STELTTVTGT NGLPTDETII VIRTPTTATT
510 520 530 540 550
AMTTTQPWND TFTSTSTEIT TVTGTNGLPT DETIIVIRTP TTATTAMTTT
560 570 580 590 600
QPWNDTFTST STEMTTVTGT NGLPTDETII VIRTPTTATT AITTTEPWNS
610 620 630 640 650
TFTSTSTEMT TVTGTNGLPT DETIIVIRTP TTATTAITTT QPWNDTFTST
660 670 680 690 700
STEMTTVTGT NGLPTDETII VIRTPTTATT AMTTTQPWND TFTSTSTEIT
710 720 730 740 750
TVTGTNGLPT DETIIVIRTP TTATTAMTTT QPWNDTFTST STEMTTVTGT
760 770 780 790 800
NGVPTDETVI VIRTPTSEGL ISTTTEPWTG TFTSTSTEMT TVTGTNGQPT
810 820 830 840 850
DETVIVIRTP TSEGLVTTTT EPWTGTFTST STEMTTITGT NGQPTDETVI
860 870 880 890 900
IVKTPTTAIS SSLSSSSGQI TSFITSARPI ITPFYPSNGT SVISSSVISS
910 920 930 940 950
SDTSSLVISS SVTSSLVTSS PVISSSFISS PVISSTTTSA SILSESSKSS
960 970 980 990 1000
VIPTSSSTSG SSESETGSAS SASSSSSISS ESPKSTYSSS SLPPVTSATT
1010 1020 1030 1040 1050
SQEITSSLPP VTTTKTSEQT TLVTVTSCES HVCTESISSA IVSTATVTVS
1060 1070 1080 1090 1100
GATTEYTTWC PISTTEITKQ TTETTKQTKG TTEQTTETTK QTTVVTISSC
1110 1120 1130 1140 1150
ESDVCSKTAS PAIVSTSTAT INGVTTEYTT WCPISTTESK QQTTLVTVTS
1160 1170 1180 1190 1200
CGSGVCSETT SPAIVSTATA TVNDVVTVYS TWRPQTTNEQ SVSSKMNSAT
1210 1220 1230 1240 1250
SETTTNTGAA ETTTSTGAAE TKTVVTSSIS RFNHAETQTA SATDVIGHSS
1260 1270 1280 1290 1300
SVVSVSETGN TKSLTSSGLS TMSQQPRSTP ASSMVGSSTA SLEISTYAGS
1310 1320
ANSLLAGSGL SVFIASLLLA II
Length:1,322
Mass (Da):138,073
Last modified:February 1, 1996 - v2
Checksum:iAADFD1FF13267CEA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12980 Genomic DNA. Translation: AAC04971.1.
BK006935 Genomic DNA. Translation: DAA06926.1.
PIRiS51959.
RefSeqiNP_009338.1. NM_001178205.1.

Genome annotation databases

EnsemblFungiiYAL063C; YAL063C; YAL063C.
GeneIDi851236.
KEGGisce:YAL063C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U12980 Genomic DNA. Translation: AAC04971.1 .
BK006935 Genomic DNA. Translation: DAA06926.1 .
PIRi S51959.
RefSeqi NP_009338.1. NM_001178205.1.

3D structure databases

ProteinModelPortali P39712.
SMRi P39712. Positions 22-269.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31767. 14 interactions.
IntActi P39712. 1 interaction.
STRINGi 4932.YAL063C.

Proteomic databases

PaxDbi P39712.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL063C ; YAL063C ; YAL063C .
GeneIDi 851236.
KEGGi sce:YAL063C.

Organism-specific databases

CYGDi YAL063c.
SGDi S000000059. FLO9.

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00660000095872.
HOGENOMi HOG000112611.
InParanoidi P39712.
OMAi TIETIYY.
OrthoDBi EOG7H4F6F.

Enzyme and pathway databases

BioCyci YEAST:G3O-28864-MONOMER.

Miscellaneous databases

NextBioi 968156.

Gene expression databases

Genevestigatori P39712.

Family and domain databases

Gene3Di 3.90.182.10. 1 hit.
InterProi IPR001389. Flocculin.
IPR025928. Flocculin_t3_rpt.
IPR011658. PA14.
[Graphical view ]
Pfami PF00624. Flocculin. 13 hits.
PF13928. Flocculin_t3. 3 hits.
PF07691. PA14. 1 hit.
[Graphical view ]
SMARTi SM00758. PA14. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Review: the dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family."
    Teunissen A.W.R.H., Steensma H.Y.
    Yeast 11:1001-1013(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  4. "Multiple sequence signals determine the distribution of glycosylphosphatidylinositol proteins between the plasma membrane and cell wall in Saccharomyces cerevisiae."
    Frieman M.B., Cormack B.P.
    Microbiology 150:3105-3114(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, REPEATS.
  5. "Intragenic tandem repeats generate functional variability."
    Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
    Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REPEATS.

Entry informationi

Entry nameiFLO9_YEAST
AccessioniPrimary (citable) accession number: P39712
Secondary accession number(s): D6VPF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3