ID DHE5_YEAST Reviewed; 457 AA. AC P39708; D6VPF7; DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot. DT 01-FEB-1995, sequence version 1. DT 27-MAR-2024, entry version 170. DE RecName: Full=NADP-specific glutamate dehydrogenase 2; DE Short=NADP-GDH 2; DE EC=1.4.1.4; DE AltName: Full=NADP-dependent glutamate dehydrogenase 2; GN Name=GDH3; OrderedLocusNames=YAL062W; ORFNames=FUN51; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=7731988; DOI=10.1073/pnas.92.9.3809; RA Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., RA Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., RA Storms R.K.; RT "The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."; RL Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995). RN [2] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RX PubMed=24374639; DOI=10.1534/g3.113.008995; RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.; RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now."; RL G3 (Bethesda) 4:389-398(2014). CC -!- CATALYTIC ACTIVITY: CC Reaction=H2O + L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + NADPH + CC NH4(+); Xref=Rhea:RHEA:11612, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:16810, ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.4.1.4; CC -!- SUBUNIT: Homohexamer. {ECO:0000250}. CC -!- SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U12980; AAC04972.1; -; Genomic_DNA. DR EMBL; BK006935; DAA06927.1; -; Genomic_DNA. DR PIR; S51960; S51960. DR RefSeq; NP_009339.1; NM_001178204.1. DR AlphaFoldDB; P39708; -. DR SMR; P39708; -. DR BioGRID; 31768; 61. DR DIP; DIP-4325N; -. DR IntAct; P39708; 2. DR MINT; P39708; -. DR STRING; 4932.YAL062W; -. DR iPTMnet; P39708; -. DR MaxQB; P39708; -. DR PaxDb; 4932-YAL062W; -. DR PeptideAtlas; P39708; -. DR EnsemblFungi; YAL062W_mRNA; YAL062W; YAL062W. DR GeneID; 851237; -. DR KEGG; sce:YAL062W; -. DR AGR; SGD:S000000058; -. DR SGD; S000000058; GDH3. DR VEuPathDB; FungiDB:YAL062W; -. DR eggNOG; KOG2250; Eukaryota. DR GeneTree; ENSGT00390000000854; -. DR HOGENOM; CLU_025763_2_1_1; -. DR InParanoid; P39708; -. DR OMA; PCFAAFP; -. DR OrthoDB; 45283at2759; -. DR BioCyc; MetaCyc:YAL062W-MONOMER; -. DR BioCyc; YEAST:YAL062W-MONOMER; -. DR BioGRID-ORCS; 851237; 10 hits in 10 CRISPR screens. DR PRO; PR:P39708; -. DR Proteomes; UP000002311; Chromosome I. DR RNAct; P39708; Protein. DR GO; GO:0005829; C:cytosol; IBA:GO_Central. DR GO; GO:0005739; C:mitochondrion; HDA:SGD. DR GO; GO:0005634; C:nucleus; IDA:SGD. DR GO; GO:0004354; F:glutamate dehydrogenase (NADP+) activity; IDA:SGD. DR GO; GO:0006537; P:glutamate biosynthetic process; IMP:SGD. DR CDD; cd05313; NAD_bind_2_Glu_DH; 1. DR Gene3D; 1.10.285.10; Glutamate Dehydrogenase, chain A, domain 3; 2. DR Gene3D; 3.40.50.10860; Leucine Dehydrogenase, chain A, domain 1; 1. DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1. DR InterPro; IPR046346; Aminoacid_DH-like_N_sf. DR InterPro; IPR006095; Glu/Leu/Phe/Val/Trp_DH. DR InterPro; IPR006096; Glu/Leu/Phe/Val/Trp_DH_C. DR InterPro; IPR006097; Glu/Leu/Phe/Val/Trp_DH_dimer. DR InterPro; IPR033524; Glu/Leu/Phe/Val_DH_AS. DR InterPro; IPR014362; Glu_DH. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR033922; NAD_bind_Glu_DH. DR PANTHER; PTHR43571; NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 1-RELATED; 1. DR PANTHER; PTHR43571:SF1; NADP-SPECIFIC GLUTAMATE DEHYDROGENASE 1-RELATED; 1. DR Pfam; PF00208; ELFV_dehydrog; 1. DR Pfam; PF02812; ELFV_dehydrog_N; 1. DR PIRSF; PIRSF000185; Glu_DH; 1. DR PRINTS; PR00082; GLFDHDRGNASE. DR SMART; SM00839; ELFV_dehydrog; 1. DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1. DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1. DR PROSITE; PS00074; GLFV_DEHYDROGENASE; 1. PE 3: Inferred from homology; KW Isopeptide bond; NADP; Oxidoreductase; Reference proteome; Ubl conjugation. FT CHAIN 1..457 FT /note="NADP-specific glutamate dehydrogenase 2" FT /id="PRO_0000182799" FT ACT_SITE 111 FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10011" FT BINDING 175..204 FT /ligand="NAD(+)" FT /ligand_id="ChEBI:CHEBI:57540" FT /evidence="ECO:0000250" FT CROSSLNK 326 FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with FT G-Cter in ubiquitin)" FT /evidence="ECO:0000250|UniProtKB:P07262" FT CROSSLNK 372 FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with FT G-Cter in ubiquitin)" FT /evidence="ECO:0000250|UniProtKB:P07262" FT CROSSLNK 436 FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with FT G-Cter in ubiquitin)" FT /evidence="ECO:0000250|UniProtKB:P07262" SQ SEQUENCE 457 AA; 49627 MW; 81B30625038B1888 CRC64; MTSEPEFQQA YDEIVSSVED SKIFEKFPQY KKVLPIVSVP ERIIQFRVTW ENDNGEQEVA QGYRVQFNSA KGPYKGGLRF HPSVNLSILK FLGFEQIFKN ALTGLDMGGG KGGLCVDLKG KSDNEIRRIC YAFMRELSRH IGKDTDVPAG DIGVGGREIG YLFGAYRSYK NSWEGVLTGK GLNWGGSLIR PEATGFGLVY YTQAMIDYAT NGKESFEGKR VTISGSGNVA QYAALKVIEL GGIVVSLSDS KGCIISETGI TSEQIHDIAS AKIRFKSLEE IVDEYSTFSE SKMKYVAGAR PWTHVSNVDI ALPCATQNEV SGDEAKALVA SGVKFVAEGA NMGSTPEAIS VFETARSTAT NAKDAVWFGP PKAANLGGVA VSGLEMAQNS QKVTWTAERV DQELKKIMIN CFNDCIQAAQ EYSTEKNTNT LPSLVKGANI ASFVMVADAM LDQGDVF //