Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADP-specific glutamate dehydrogenase 2

Gene

GDH3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei111 – 1111PROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi175 – 20430NADBy similarityAdd
BLAST

GO - Molecular functioni

  • glutamate dehydrogenase (NADP+) activity Source: SGD

GO - Biological processi

  • ammonia assimilation cycle Source: SGD
  • glutamate biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11505.
YEAST:YAL062W-MONOMER.
ReactomeiREACT_288999. Amino acid synthesis and interconversion (transamination).

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase 2 (EC:1.4.1.4)
Short name:
NADP-GDH 2
Alternative name(s):
NADP-dependent glutamate dehydrogenase 2
Gene namesi
Name:GDH3
Ordered Locus Names:YAL062W
ORF Names:FUN51
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome I

Organism-specific databases

CYGDiYAL062w.
EuPathDBiFungiDB:YAL062W.
SGDiS000000058. GDH3.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457NADP-specific glutamate dehydrogenase 2PRO_0000182799Add
BLAST

Proteomic databases

MaxQBiP39708.
PaxDbiP39708.
PeptideAtlasiP39708.
PRIDEiP39708.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

BioGridi31768. 24 interactions.
DIPiDIP-4325N.
IntActiP39708. 3 interactions.
MINTiMINT-488220.
STRINGi4932.YAL062W.

Structurei

3D structure databases

ProteinModelPortaliP39708.
SMRiP39708. Positions 3-454.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0334.
GeneTreeiENSGT00390000000854.
HOGENOMiHOG000243799.
InParanoidiP39708.
KOiK00262.
OMAiSHYVNIM.
OrthoDBiEOG7XSTPW.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39708-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSEPEFQQA YDEIVSSVED SKIFEKFPQY KKVLPIVSVP ERIIQFRVTW
60 70 80 90 100
ENDNGEQEVA QGYRVQFNSA KGPYKGGLRF HPSVNLSILK FLGFEQIFKN
110 120 130 140 150
ALTGLDMGGG KGGLCVDLKG KSDNEIRRIC YAFMRELSRH IGKDTDVPAG
160 170 180 190 200
DIGVGGREIG YLFGAYRSYK NSWEGVLTGK GLNWGGSLIR PEATGFGLVY
210 220 230 240 250
YTQAMIDYAT NGKESFEGKR VTISGSGNVA QYAALKVIEL GGIVVSLSDS
260 270 280 290 300
KGCIISETGI TSEQIHDIAS AKIRFKSLEE IVDEYSTFSE SKMKYVAGAR
310 320 330 340 350
PWTHVSNVDI ALPCATQNEV SGDEAKALVA SGVKFVAEGA NMGSTPEAIS
360 370 380 390 400
VFETARSTAT NAKDAVWFGP PKAANLGGVA VSGLEMAQNS QKVTWTAERV
410 420 430 440 450
DQELKKIMIN CFNDCIQAAQ EYSTEKNTNT LPSLVKGANI ASFVMVADAM

LDQGDVF
Length:457
Mass (Da):49,627
Last modified:February 1, 1995 - v1
Checksum:i81B30625038B1888
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. Translation: AAC04972.1.
BK006935 Genomic DNA. Translation: DAA06927.1.
PIRiS51960.
RefSeqiNP_009339.1. NM_001178204.1.

Genome annotation databases

EnsemblFungiiYAL062W; YAL062W; YAL062W.
GeneIDi851237.
KEGGisce:YAL062W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12980 Genomic DNA. Translation: AAC04972.1.
BK006935 Genomic DNA. Translation: DAA06927.1.
PIRiS51960.
RefSeqiNP_009339.1. NM_001178204.1.

3D structure databases

ProteinModelPortaliP39708.
SMRiP39708. Positions 3-454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31768. 24 interactions.
DIPiDIP-4325N.
IntActiP39708. 3 interactions.
MINTiMINT-488220.
STRINGi4932.YAL062W.

Proteomic databases

MaxQBiP39708.
PaxDbiP39708.
PeptideAtlasiP39708.
PRIDEiP39708.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL062W; YAL062W; YAL062W.
GeneIDi851237.
KEGGisce:YAL062W.

Organism-specific databases

CYGDiYAL062w.
EuPathDBiFungiDB:YAL062W.
SGDiS000000058. GDH3.

Phylogenomic databases

eggNOGiCOG0334.
GeneTreeiENSGT00390000000854.
HOGENOMiHOG000243799.
InParanoidiP39708.
KOiK00262.
OMAiSHYVNIM.
OrthoDBiEOG7XSTPW.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11505.
YEAST:YAL062W-MONOMER.
ReactomeiREACT_288999. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

NextBioi968159.
PROiP39708.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiDHE5_YEAST
AccessioniPrimary (citable) accession number: P39708
Secondary accession number(s): D6VPF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 24, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.