Reviewed, UniProtKB/Swiss-Prot P39708 (DHE5_YEAST)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase 2
      Short name=NADP-GDH 2
    EC=1.4.1.4
Alternative name(s):
    NADP-dependent glutamate dehydrogenase 2

Gene names

Name:	GDH3
Ordered Locus Names:	YAL062W
ORF Names:	FUN51

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate + NH(3) + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ammonia assimilation cycle Inferred from expression pattern. Source: SGD glutamate biosynthetic process Inferred from genetic interaction. Source: SGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD soluble fraction Inferred from direct assay. Source: SGD
Molecular function	glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BOP2	Q06150	1	EBI-5832,EBI-34293

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	NADP-specific glutamate dehydrogenase 2		PRO_0000182799
Regions
	Nucleotide binding	175 – 204	30	NAD By similarity
Sites
	Active site	111	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39708-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 81B30625038B1888	FASTA	457	49,627
	10 20 30 40 50 60 MTSEPEFQQA YDEIVSSVED SKIFEKFPQY KKVLPIVSVP ERIIQFRVTW ENDNGEQEVA 70 80 90 100 110 120 QGYRVQFNSA KGPYKGGLRF HPSVNLSILK FLGFEQIFKN ALTGLDMGGG KGGLCVDLKG 130 140 150 160 170 180 KSDNEIRRIC YAFMRELSRH IGKDTDVPAG DIGVGGREIG YLFGAYRSYK NSWEGVLTGK 190 200 210 220 230 240 GLNWGGSLIR PEATGFGLVY YTQAMIDYAT NGKESFEGKR VTISGSGNVA QYAALKVIEL 250 260 270 280 290 300 GGIVVSLSDS KGCIISETGI TSEQIHDIAS AKIRFKSLEE IVDEYSTFSE SKMKYVAGAR 310 320 330 340 350 360 PWTHVSNVDI ALPCATQNEV SGDEAKALVA SGVKFVAEGA NMGSTPEAIS VFETARSTAT 370 380 390 400 410 420 NAKDAVWFGP PKAANLGGVA VSGLEMAQNS QKVTWTAERV DQELKKIMIN CFNDCIQAAQ 430 440 450 EYSTEKNTNT LPSLVKGANI ASFVMVADAM LDQGDVF

References

[1]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U12980 Genomic DNA. Translation: AAC04972.1.
PIR	S51960.
RefSeq	NP_009339.1.
3D structure databases
HSSP	HSSP built from PDB template 1AUP based on UniProtKB P24295.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:4325N.
IntAct	P39708.
Proteomic databases
PeptideAtlas	P39708.
Genome annotation databases
Ensembl	YAL062W. Saccharomyces cerevisiae. [Contig view]
GeneID	851237.
GenomeReviews	Gene locus YAL062W in contig U00091_GR.
KEGG	sce:YAL062W.
NMPDR	fig|4932.3.peg.13.
Organism-specific databases
CYGD	YAL062w.
SGD	S000000058. GDH3.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P39708.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-11505.
Gene expression databases
ArrayExpress	P39708.
GermOnline	YAL062W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DHase. IPR006096. Glu/Leu/Phe/Val_DHase_C. IPR006097. Glu/Leu/Phe/Val_DHase_dimer. IPR014362. Glu_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P39708.
NextBio	968159.

Entry information

Entry name

DHE5_YEAST

Accession

Primary (citable) accession number: P39708

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 25, 2008
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)