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P39707 (SEN34_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease subunit SEN34

EC=4.6.1.16
Alternative name(s):
Splicing endonuclease of 34 kDa
tRNA-intron endonuclease SEN34
Gene names
Name:SEN34
Ordered Locus Names:YAR008W
ORF Names:FUN4
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage.

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Subunit structure

Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN15. Ref.4

Subcellular location

Nucleus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note: The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface. Ref.5

Miscellaneous

The tRNA splicing endonuclease complex is present with 100 molecules/cell.

Sequence similarities

Belongs to the tRNA-intron endonuclease family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 275275tRNA-splicing endonuclease subunit SEN34
PRO_0000109468

Sites

Active site2091 By similarity
Active site2171
Active site2501 By similarity

Experimental info

Mutagenesis2171H → A: Loss of function; induces a marked accumulation of 5' exon and intron-3' exon 2/3 molecule. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P39707 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: CCEA2A30209AB03F

FASTA27531,313
        10         20         30         40         50         60 
MPPLVFDIDH IKLLRKWGIC GVLSGTLPTA AQQNVFLSVP LRLMLEDVLW LHLNNLADVK 

        70         80         90        100        110        120 
LIRQEGDEIM EGITLERGAK LSKIVNDRLN KSFEYQRKFK KDEHIAKLKK IGRINDKTTA 

       130        140        150        160        170        180 
EELQRLDKSS NNDQLIESSL FIDIANTSMI LRDIRSDSDS LSRDDISDLL FKQYRQAGKM 

       190        200        210        220        230        240 
QTYFLYKALR DQGYVLSPGG RFGGKFIAYP GDPLRFHSHL TIQDAIDYHN EPIDLISMIS 

       250        260        270 
GARLGTTVKK LWVIGGVAEE TKETHFFSIE WAGFG 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases."
Trotta C.R., Miao F., Arn E.A., Stevens S.W., Ho C.K., Rauhut R., Abelson J.N.
Cell 89:849-858(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 206-223, SUBUNIT, MUTAGENESIS OF HIS-217.
[5]"Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria."
Yoshihisa T., Yunoki-Esaki K., Ohshima C., Tanaka N., Endo T.
Mol. Biol. Cell 14:3266-3279(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L22015 Genomic DNA. Translation: AAC04961.1.
BK006935 Genomic DNA. Translation: DAA06990.1.
PIRS40903.
RefSeqNP_009405.1. NM_001178212.1.

3D structure databases

ProteinModelPortalP39707.
SMRP39707. Positions 181-272.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31794. 8 interactions.
DIPDIP-3794N.
IntActP39707. 3 interactions.
MINTMINT-488800.
STRING4932.YAR008W.

Proteomic databases

MaxQBP39707.
PaxDbP39707.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAR008W; YAR008W; YAR008W.
GeneID851267.
KEGGsce:YAR008W.

Organism-specific databases

CYGDYAR008w.
SGDS000000066. SEN34.

Phylogenomic databases

eggNOGCOG1676.
GeneTreeENSGT00390000003912.
HOGENOMHOG000175584.
KOK15323.
OMAEESSCHA.
OrthoDBEOG7N905X.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16150.
YEAST:G3O-28870-MONOMER.

Gene expression databases

GenevestigatorP39707.

Family and domain databases

Gene3D3.40.1350.10. 1 hit.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006676. tRNA_splic.
IPR016690. tRNA_splic_SEN34.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
PIRSFPIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAMSSF53032. SSF53032. 1 hit.
TIGRFAMsTIGR00324. endA. 1 hit.
ProtoNetSearch...

Other

NextBio968237.
PROP39707.

Entry information

Entry nameSEN34_YEAST
AccessionPrimary (citable) accession number: P39707
Secondary accession number(s): D6VPM0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families