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P39707

- SEN34_YEAST

UniProt

P39707 - SEN34_YEAST

Protein

tRNA-splicing endonuclease subunit SEN34

Gene

SEN34

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage.

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei209 – 2091By similarity
    Active sitei217 – 2171
    Active sitei250 – 2501By similarity

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: SGD

    GO - Biological processi

    1. RNA phosphodiester bond hydrolysis Source: GOC
    2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
    3. tRNA-type intron splice site recognition and cleavage Source: SGD

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-16150.
    YEAST:G3O-28870-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonuclease subunit SEN34 (EC:4.6.1.16)
    Alternative name(s):
    Splicing endonuclease of 34 kDa
    tRNA-intron endonuclease SEN34
    Gene namesi
    Name:SEN34
    Ordered Locus Names:YAR008W
    ORF Names:FUN4
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAR008w.
    SGDiS000000066. SEN34.

    Subcellular locationi

    Nucleus 1 Publication. Endomembrane system 1 Publication; Peripheral membrane protein 1 Publication. Mitochondrion outer membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
    Note: The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface.

    GO - Cellular componenti

    1. endomembrane system Source: UniProtKB-SubCell
    2. mitochondrial outer membrane Source: UniProtKB-SubCell
    3. tRNA-intron endonuclease complex Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi217 – 2171H → A: Loss of function; induces a marked accumulation of 5' exon and intron-3' exon 2/3 molecule. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 275275tRNA-splicing endonuclease subunit SEN34PRO_0000109468Add
    BLAST

    Proteomic databases

    MaxQBiP39707.
    PaxDbiP39707.

    Expressioni

    Gene expression databases

    GenevestigatoriP39707.

    Interactioni

    Subunit structurei

    Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN15.1 Publication

    Protein-protein interaction databases

    BioGridi31794. 8 interactions.
    DIPiDIP-3794N.
    IntActiP39707. 3 interactions.
    MINTiMINT-488800.
    STRINGi4932.YAR008W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39707.
    SMRiP39707. Positions 181-272.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family.Curated

    Phylogenomic databases

    eggNOGiCOG1676.
    GeneTreeiENSGT00390000003912.
    HOGENOMiHOG000175584.
    KOiK15323.
    OMAiEESSCHA.
    OrthoDBiEOG7N905X.

    Family and domain databases

    Gene3Di3.40.1350.10. 1 hit.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006676. tRNA_splic.
    IPR016690. tRNA_splic_SEN34.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017250. tRNA_splic_SEN34. 1 hit.
    SUPFAMiSSF53032. SSF53032. 1 hit.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P39707-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPLVFDIDH IKLLRKWGIC GVLSGTLPTA AQQNVFLSVP LRLMLEDVLW    50
    LHLNNLADVK LIRQEGDEIM EGITLERGAK LSKIVNDRLN KSFEYQRKFK 100
    KDEHIAKLKK IGRINDKTTA EELQRLDKSS NNDQLIESSL FIDIANTSMI 150
    LRDIRSDSDS LSRDDISDLL FKQYRQAGKM QTYFLYKALR DQGYVLSPGG 200
    RFGGKFIAYP GDPLRFHSHL TIQDAIDYHN EPIDLISMIS GARLGTTVKK 250
    LWVIGGVAEE TKETHFFSIE WAGFG 275
    Length:275
    Mass (Da):31,313
    Last modified:February 1, 1995 - v1
    Checksum:iCCEA2A30209AB03F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22015 Genomic DNA. Translation: AAC04961.1.
    BK006935 Genomic DNA. Translation: DAA06990.1.
    PIRiS40903.
    RefSeqiNP_009405.1. NM_001178212.1.

    Genome annotation databases

    EnsemblFungiiYAR008W; YAR008W; YAR008W.
    GeneIDi851267.
    KEGGisce:YAR008W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22015 Genomic DNA. Translation: AAC04961.1 .
    BK006935 Genomic DNA. Translation: DAA06990.1 .
    PIRi S40903.
    RefSeqi NP_009405.1. NM_001178212.1.

    3D structure databases

    ProteinModelPortali P39707.
    SMRi P39707. Positions 181-272.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31794. 8 interactions.
    DIPi DIP-3794N.
    IntActi P39707. 3 interactions.
    MINTi MINT-488800.
    STRINGi 4932.YAR008W.

    Proteomic databases

    MaxQBi P39707.
    PaxDbi P39707.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAR008W ; YAR008W ; YAR008W .
    GeneIDi 851267.
    KEGGi sce:YAR008W.

    Organism-specific databases

    CYGDi YAR008w.
    SGDi S000000066. SEN34.

    Phylogenomic databases

    eggNOGi COG1676.
    GeneTreei ENSGT00390000003912.
    HOGENOMi HOG000175584.
    KOi K15323.
    OMAi EESSCHA.
    OrthoDBi EOG7N905X.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-16150.
    YEAST:G3O-28870-MONOMER.

    Miscellaneous databases

    NextBioi 968237.
    PROi P39707.

    Gene expression databases

    Genevestigatori P39707.

    Family and domain databases

    Gene3Di 3.40.1350.10. 1 hit.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006676. tRNA_splic.
    IPR016690. tRNA_splic_SEN34.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF017250. tRNA_splic_SEN34. 1 hit.
    SUPFAMi SSF53032. SSF53032. 1 hit.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
      Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
      Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases."
      Trotta C.R., Miao F., Arn E.A., Stevens S.W., Ho C.K., Rauhut R., Abelson J.N.
      Cell 89:849-858(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 206-223, SUBUNIT, MUTAGENESIS OF HIS-217.
    5. "Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria."
      Yoshihisa T., Yunoki-Esaki K., Ohshima C., Tanaka N., Endo T.
      Mol. Biol. Cell 14:3266-3279(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiSEN34_YEAST
    AccessioniPrimary (citable) accession number: P39707
    Secondary accession number(s): D6VPM0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The tRNA splicing endonuclease complex is present with 100 molecules/cell.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3