P39707 (SEN34_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease subunit SEN34
EC=3.1.27.9

Alternative name(s):
Splicing endonuclease of 34 kDa
tRNA-intron endonuclease SEN34

Gene names

Name:	SEN34
Ordered Locus Names:	YAR008W
ORF Names:	FUN4

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage.
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron.
Subunit structure	Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN15. Ref.4
Subcellular location	Nucleus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Note: The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface. Ref.5
Miscellaneous	The tRNA splicing endonuclease complex is present with 100 molecules/cell.
Sequence similarities	Belongs to the tRNA-intron endonuclease family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane Nucleus
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	tRNA-type intron splice site recognition and cleavage Inferred from direct assay Ref.4. Source: SGD
Cellular component	endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from direct assay. Source: SGD tRNA-intron endonuclease complex Inferred from direct assay Ref.4. Source: SGD
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from mutant phenotype Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	tRNA-splicing endonuclease subunit SEN34		PRO_0000109468
Sites
	Active site	209	1	By similarity
	Active site	217	1
	Active site	250	1	By similarity
Experimental info
	Mutagenesis	217	1	H → A: Loss of function; induces a marked accumulation of 5' exon and intron-3' exon 2/3 molecule. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P39707 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: CCEA2A30209AB03F	FASTA	275	31,313
	10 20 30 40 50 60 MPPLVFDIDH IKLLRKWGIC GVLSGTLPTA AQQNVFLSVP LRLMLEDVLW LHLNNLADVK 70 80 90 100 110 120 LIRQEGDEIM EGITLERGAK LSKIVNDRLN KSFEYQRKFK KDEHIAKLKK IGRINDKTTA 130 140 150 160 170 180 EELQRLDKSS NNDQLIESSL FIDIANTSMI LRDIRSDSDS LSRDDISDLL FKQYRQAGKM 190 200 210 220 230 240 QTYFLYKALR DQGYVLSPGG RFGGKFIAYP GDPLRFHSHL TIQDAIDYHN EPIDLISMIS 250 260 270 GARLGTTVKK LWVIGGVAEE TKETHFFSIE WAGFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region." Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H. Yeast 10:535-541(1994) [PubMed: 7941740] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases." Trotta C.R., Miao F., Arn E.A., Stevens S.W., Ho C.K., Rauhut R., Abelson J.N. Cell 89:849-858(1997) [PubMed: 9200603] [Abstract] Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 206-223, SUBUNIT, MUTAGENESIS OF HIS-217.
[5]	"Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria." Yoshihisa T., Yunoki-Esaki K., Ohshima C., Tanaka N., Endo T. Mol. Biol. Cell 14:3266-3279(2003) [PubMed: 12925762] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L22015 Genomic DNA. Translation: AAC04961.1. BK006935 Genomic DNA. Translation: DAA06990.1.
PIR	S40903.
RefSeq	NP_009405.1. NM_001178212.1.
3D structure databases
ProteinModelPortal	P39707.
SMR	P39707. Positions 180-274.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3794N.
IntAct	P39707. 3 interactions.
MINT	MINT-488800.
STRING	P39707.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAR008W; YAR008W; YAR008W.
GeneID	851267.
KEGG	sce:YAR008W.
NMPDR	fig|4932.3.peg.89.
Organism-specific databases
CYGD	YAR008w.
SGD	S000000066. SEN34.
Phylogenomic databases
eggNOG	fuNOG09448.
GeneTree	EFGT00050000006727.
HOGENOM	HBG396297.
OMA	RYVAYPG.
OrthoDB	EOG4WDHN7.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-16150.
Gene expression databases
ArrayExpress	P39707.
Genevestigator	P39707.
GermOnline	YAR008W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011856. Endonuc_TnsA/Hjc/tRNA. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006676. tRNA_splic. IPR016690. tRNA_splic_SEN34. [Graphical view]
Gene3D	G3DSA:3.40.1350.10. Endonuc_TnsA/Hjc/tRNA. 1 hit.
KO	K15323.
Pfam	PF01974. tRNA_int_endo. 1 hit. [Graphical view]
PIRSF	PIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAM	SSF53032. tRNA_int_endo_C. 1 hit.
TIGRFAMs	TIGR00324. EndA. 1 hit.
ProtoNet	Search...
Other
NextBio	968237.

Entry information

Entry name

SEN34_YEAST

Accession

Primary (citable) accession number: P39707
Secondary accession number(s): D6VPM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program