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Protein

tRNA-splicing endonuclease subunit SEN34

Gene

SEN34

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage.

Miscellaneous

The tRNA splicing endonuclease complex is present with 100 molecules/cell.

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei209By similarity1
Active sitei2171
Active sitei250By similarity1

GO - Molecular functioni

  • lyase activity Source: UniProtKB-KW
  • nucleic acid binding Source: InterPro
  • tRNA-intron endonuclease activity Source: SGD

GO - Biological processi

  • tRNA-type intron splice site recognition and cleavage Source: SGD

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Enzyme and pathway databases

BioCyciMetaCyc:G3O-28870-MONOMER
YEAST:G3O-28870-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit SEN34 (EC:4.6.1.16)
Alternative name(s):
Splicing endonuclease of 34 kDa
tRNA-intron endonuclease SEN34
Gene namesi
Name:SEN34
Ordered Locus Names:YAR008W
ORF Names:FUN4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAR008W
SGDiS000000066 SEN34

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi217H → A: Loss of function; induces a marked accumulation of 5' exon and intron-3' exon 2/3 molecule. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094681 – 275tRNA-splicing endonuclease subunit SEN34Add BLAST275

Proteomic databases

MaxQBiP39707
PaxDbiP39707
PRIDEiP39707

Interactioni

Subunit structurei

Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN15.1 Publication

Protein-protein interaction databases

BioGridi31794, 13 interactors
ComplexPortaliCPX-1832 tRNA-intron endonuclease complex
DIPiDIP-3794N
IntActiP39707, 4 interactors
STRINGi4932.YAR008W

Structurei

3D structure databases

ProteinModelPortaliP39707
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000003912
HOGENOMiHOG000175584
InParanoidiP39707
KOiK15323
OMAiRTFSIEW
OrthoDBiEOG092C3X38

Family and domain databases

Gene3Di3.40.1350.10, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006676 tRNA_splic
IPR016690 tRNA_splic_SEN34
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PIRSFiPIRSF017250 tRNA_splic_SEN34, 1 hit
SUPFAMiSSF53032 SSF53032, 1 hit
TIGRFAMsiTIGR00324 endA, 1 hit

Sequencei

Sequence statusi: Complete.

P39707-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPLVFDIDH IKLLRKWGIC GVLSGTLPTA AQQNVFLSVP LRLMLEDVLW
60 70 80 90 100
LHLNNLADVK LIRQEGDEIM EGITLERGAK LSKIVNDRLN KSFEYQRKFK
110 120 130 140 150
KDEHIAKLKK IGRINDKTTA EELQRLDKSS NNDQLIESSL FIDIANTSMI
160 170 180 190 200
LRDIRSDSDS LSRDDISDLL FKQYRQAGKM QTYFLYKALR DQGYVLSPGG
210 220 230 240 250
RFGGKFIAYP GDPLRFHSHL TIQDAIDYHN EPIDLISMIS GARLGTTVKK
260 270
LWVIGGVAEE TKETHFFSIE WAGFG
Length:275
Mass (Da):31,313
Last modified:February 1, 1995 - v1
Checksum:iCCEA2A30209AB03F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22015 Genomic DNA Translation: AAC04961.1
BK006935 Genomic DNA Translation: DAA06990.1
PIRiS40903
RefSeqiNP_009405.1, NM_001178212.1

Genome annotation databases

EnsemblFungiiYAR008W; YAR008W; YAR008W
GeneIDi851267
KEGGisce:YAR008W

Similar proteinsi

Entry informationi

Entry nameiSEN34_YEAST
AccessioniPrimary (citable) accession number: P39707
Secondary accession number(s): D6VPM0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 20, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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