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P39707

- SEN34_YEAST

UniProt

P39707 - SEN34_YEAST

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Protein

tRNA-splicing endonuclease subunit SEN34

Gene
SEN34, YAR008W, FUN4
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage.

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei209 – 2091 By similarity
Active sitei217 – 2171
Active sitei250 – 2501 By similarity

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: SGD

GO - Biological processi

  1. RNA phosphodiester bond hydrolysis Source: GOC
  2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  3. tRNA-type intron splice site recognition and cleavage Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16150.
YEAST:G3O-28870-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonuclease subunit SEN34 (EC:4.6.1.16)
Alternative name(s):
Splicing endonuclease of 34 kDa
tRNA-intron endonuclease SEN34
Gene namesi
Name:SEN34
Ordered Locus Names:YAR008W
ORF Names:FUN4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAR008w.
SGDiS000000066. SEN34.

Subcellular locationi

Nucleus. Endomembrane system; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side
Note: The tRNA splicing endonuclease complex is predominantly associated with the outer membrane of mitochondria, suggesting that tRNA splicing mainly takes place on the mitochondrial surface.1 Publication

GO - Cellular componenti

  1. endomembrane system Source: UniProtKB-SubCell
  2. mitochondrial outer membrane Source: UniProtKB-SubCell
  3. tRNA-intron endonuclease complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi217 – 2171H → A: Loss of function; induces a marked accumulation of 5' exon and intron-3' exon 2/3 molecule. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275tRNA-splicing endonuclease subunit SEN34PRO_0000109468Add
BLAST

Proteomic databases

MaxQBiP39707.
PaxDbiP39707.

Expressioni

Gene expression databases

GenevestigatoriP39707.

Interactioni

Subunit structurei

Heterotetramer composed of SEN2, SEN15, SEN34 and SEN54. Interacts directly with SEN15.1 Publication

Protein-protein interaction databases

BioGridi31794. 8 interactions.
DIPiDIP-3794N.
IntActiP39707. 3 interactions.
MINTiMINT-488800.
STRINGi4932.YAR008W.

Structurei

3D structure databases

ProteinModelPortaliP39707.
SMRiP39707. Positions 181-272.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1676.
GeneTreeiENSGT00390000003912.
HOGENOMiHOG000175584.
KOiK15323.
OMAiEESSCHA.
OrthoDBiEOG7N905X.

Family and domain databases

Gene3Di3.40.1350.10. 1 hit.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006676. tRNA_splic.
IPR016690. tRNA_splic_SEN34.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 1 hit.
[Graphical view]
PIRSFiPIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAMiSSF53032. SSF53032. 1 hit.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

P39707-1 [UniParc]FASTAAdd to Basket

« Hide

MPPLVFDIDH IKLLRKWGIC GVLSGTLPTA AQQNVFLSVP LRLMLEDVLW    50
LHLNNLADVK LIRQEGDEIM EGITLERGAK LSKIVNDRLN KSFEYQRKFK 100
KDEHIAKLKK IGRINDKTTA EELQRLDKSS NNDQLIESSL FIDIANTSMI 150
LRDIRSDSDS LSRDDISDLL FKQYRQAGKM QTYFLYKALR DQGYVLSPGG 200
RFGGKFIAYP GDPLRFHSHL TIQDAIDYHN EPIDLISMIS GARLGTTVKK 250
LWVIGGVAEE TKETHFFSIE WAGFG 275
Length:275
Mass (Da):31,313
Last modified:February 1, 1995 - v1
Checksum:iCCEA2A30209AB03F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L22015 Genomic DNA. Translation: AAC04961.1.
BK006935 Genomic DNA. Translation: DAA06990.1.
PIRiS40903.
RefSeqiNP_009405.1. NM_001178212.1.

Genome annotation databases

EnsemblFungiiYAR008W; YAR008W; YAR008W.
GeneIDi851267.
KEGGisce:YAR008W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L22015 Genomic DNA. Translation: AAC04961.1 .
BK006935 Genomic DNA. Translation: DAA06990.1 .
PIRi S40903.
RefSeqi NP_009405.1. NM_001178212.1.

3D structure databases

ProteinModelPortali P39707.
SMRi P39707. Positions 181-272.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31794. 8 interactions.
DIPi DIP-3794N.
IntActi P39707. 3 interactions.
MINTi MINT-488800.
STRINGi 4932.YAR008W.

Proteomic databases

MaxQBi P39707.
PaxDbi P39707.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAR008W ; YAR008W ; YAR008W .
GeneIDi 851267.
KEGGi sce:YAR008W.

Organism-specific databases

CYGDi YAR008w.
SGDi S000000066. SEN34.

Phylogenomic databases

eggNOGi COG1676.
GeneTreei ENSGT00390000003912.
HOGENOMi HOG000175584.
KOi K15323.
OMAi EESSCHA.
OrthoDBi EOG7N905X.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-16150.
YEAST:G3O-28870-MONOMER.

Miscellaneous databases

NextBioi 968237.
PROi P39707.

Gene expression databases

Genevestigatori P39707.

Family and domain databases

Gene3Di 3.40.1350.10. 1 hit.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006676. tRNA_splic.
IPR016690. tRNA_splic_SEN34.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 1 hit.
[Graphical view ]
PIRSFi PIRSF017250. tRNA_splic_SEN34. 1 hit.
SUPFAMi SSF53032. SSF53032. 1 hit.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
    Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
    Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The yeast tRNA splicing endonuclease: a tetrameric enzyme with two active site subunits homologous to the archaeal tRNA endonucleases."
    Trotta C.R., Miao F., Arn E.A., Stevens S.W., Ho C.K., Rauhut R., Abelson J.N.
    Cell 89:849-858(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 206-223, SUBUNIT, MUTAGENESIS OF HIS-217.
  5. "Possibility of cytoplasmic pre-tRNA splicing: the yeast tRNA splicing endonuclease mainly localizes on the mitochondria."
    Yoshihisa T., Yunoki-Esaki K., Ohshima C., Tanaka N., Endo T.
    Mol. Biol. Cell 14:3266-3279(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSEN34_YEAST
AccessioniPrimary (citable) accession number: P39707
Secondary accession number(s): D6VPM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The tRNA splicing endonuclease complex is present with 100 molecules/cell.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

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