Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nucleoporin NUP60

Gene

NUP60

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm).4 Publications

Miscellaneous

Present with 4590 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • phospholipid binding Source: SGD
  • structural constituent of nuclear pore Source: SGD

GO - Biological processi

  • chromatin silencing at silent mating-type cassette Source: SGD
  • chromosome organization Source: SGD
  • double-strand break repair Source: SGD
  • intracellular mRNA localization Source: SGD
  • mRNA export from nucleus in response to heat stress Source: SGD
  • NLS-bearing protein import into nucleus Source: SGD
  • nucleocytoplasmic transport Source: SGD
  • poly(A)+ mRNA export from nucleus Source: SGD
  • posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
  • protein export from nucleus Source: SGD
  • regulation of protein desumoylation Source: SGD
  • telomere tethering at nuclear periphery Source: SGD
  • transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD

Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-28867-MONOMER

Protein family/group databases

TCDBi1.I.1.1.1 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP60
Alternative name(s):
Nuclear pore protein NUP60
Gene namesi
Name:NUP60
Synonyms:FUN17
Ordered Locus Names:YAR002W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAR002W
SGDiS000000063 NUP60

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048791 – 539Nucleoporin NUP60Add BLAST539

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineCombined sources1
Modified residuei49PhosphoserineCombined sources1
Modified residuei81PhosphoserineCombined sources1
Modified residuei89PhosphoserineCombined sources1
Modified residuei162PhosphoserineCombined sources1
Modified residuei171PhosphoserineCombined sources1
Modified residuei214PhosphoserineCombined sources1
Modified residuei222PhosphoserineCombined sources1
Modified residuei352PhosphoserineCombined sources1
Modified residuei360PhosphoserineCombined sources1
Modified residuei374PhosphoserineCombined sources1
Modified residuei382PhosphoserineCombined sources1
Modified residuei460PhosphothreonineCombined sources1
Modified residuei480PhosphoserineCombined sources1
Modified residuei483PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by CDC28.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39705
PaxDbiP39705
PRIDEiP39705

PTM databases

iPTMnetiP39705

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEC13 and SEH1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Binds to NUP1 and NUP2 forming the nuclear basket and the distal ring. The interaction with NUP2 is GSP1-GTP-dependent. Interacts through its FG repeats with karyopherins, such as KAP123 and KAP95-SRP1 (KAP60). Also interacts with GSP1-GTP and SRM1 (PRP20), where NUP60 reduces SRM1 activity, thus inhibiting GSP1 guanine nucleotide dissociation.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31790, 347 interactors
DIPiDIP-5821N
IntActiP39705, 12 interactors
MINTiP39705
STRINGi4932.YAR002W

Structurei

3D structure databases

ProteinModelPortaliP39705
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati399 – 401FXF 13
Repeati427 – 429FXF 23
Repeati469 – 471FXF 33
Repeati509 – 511FXF 43

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili91 – 118Sequence analysisAdd BLAST28

Domaini

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited. FG repeat types and their physico-chemical environment change across the NPC from the nucleoplasmic to the cytoplasmic side.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

InParanoidiP39705
KOiK18718
OMAiIEYEGVM
OrthoDBiEOG092C2FN5

Family and domain databases

InterProiView protein in InterPro
IPR034432 Nup60
PANTHERiPTHR28284 PTHR28284, 1 hit

Sequencei

Sequence statusi: Complete.

P39705-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRKSLRRAS ATVPSAPYRK QIISNAHNKP SLFSKIKTFF TQKDSARVSP
60 70 80 90 100
RNNVANKQPR NESFNRRISS MPGGYFHSEI SPDSTVNRSV VVSAVGEARN
110 120 130 140 150
DIENKEEEYD ETHETNISNA KLANFFSKKG NEPLSEIEIE GVMSLLQKSS
160 170 180 190 200
KSMITSEGEQ KSAEGNNIDQ SLILKESGST PISISNAPTF NPKYDTSNAS
210 220 230 240 250
MNTTLGSIGS RKYSFNYSSL PSPYKTTVYR YSAAKKIPDT YTANTSAQSI
260 270 280 290 300
ASAKSVRSGV SKSAPSKKIS NTAAALVSLL DENDSKKNNA ASELANPYSS
310 320 330 340 350
YVSQIRKHKR VSPNAAPRQE ISEEETTVKP LFQNVPEQGE EPMKQLNATK
360 370 380 390 400
ISPSAPSKDS FTKYKPARSS SLRSNVVVAE TSPEKKDGGD KPPSSAFNFS
410 420 430 440 450
FNTSRNVEPT ENAYKSENAP SASSKEFNFT NLQAKPLVGK PKTELTKGDS
460 470 480 490 500
TPVQPDLSVT PQKSSSKGFV FNSVQKKSRS NLSQENDNEG KHISASIDND
510 520 530
FSEEKAEEFD FNVPVVSKQL GNGLVDENKV EAFKSLYTF
Length:539
Mass (Da):59,039
Last modified:February 1, 1995 - v1
Checksum:iB9A28494A358A3E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22015 Genomic DNA Translation: AAC04957.1
AY692943 Genomic DNA Translation: AAT92962.1
BK006935 Genomic DNA Translation: DAA06986.1
PIRiS40900
RefSeqiNP_009401.1, NM_001178209.1

Genome annotation databases

EnsemblFungiiYAR002W; YAR002W; YAR002W
GeneIDi851263
KEGGisce:YAR002W

Similar proteinsi

Entry informationi

Entry nameiNUP60_YEAST
AccessioniPrimary (citable) accession number: P39705
Secondary accession number(s): D6VPL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Protein nomenclature information
    List of publications dealing with protein nomenclature and links to specialized websites
  2. Strains
    Controlled vocabulary of strains

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health