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P39702 (VPS8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar protein sorting-associated protein 8
Alternative name(s):
Vacuolar protein-targeting protein 8
Gene names
Name:VPS8
Synonyms:VPT8
Ordered Locus Names:YAL002W
ORF Names:FUN15
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1274 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for localization and recycling of the CPY sorting receptor (VPS10) to the late-Golgi compartment. Involved in the retention of proteins to the late-Golgi. Plays an integral role in the complex vacuolar protein sorting process.

Subcellular location

Golgi apparatusGolgi stack. Note: Associated with the late-Golgi membranes.

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the VPS8 family.

Contains 2 CHCR (clathrin heavy-chain) repeats.

Contains 1 RING-type zinc finger.

Contains 3 WD repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12741274Vacuolar protein sorting-associated protein 8
PRO_0000055901

Regions

Repeat75 – 11945WD 1
Repeat131 – 17040WD 2
Repeat193 – 23341WD 3
Repeat507 – 665159CHCR 1
Repeat915 – 1092178CHCR 2
Zinc finger1198 – 126669RING-type; atypical
Compositional bias40 – 434Poly-Ser

Amino acid modifications

Modified residue11N-acetylmethionine Ref.7

Sequences

Sequence LengthMass (Da)Tools
P39702 [UniParc].

Last modified July 19, 2004. Version 2.
Checksum: E4476C7C64BFF2CE

FASTA1,274144,962
        10         20         30         40         50         60 
MEQNGLDHDS RSSIDTTIND TQKTFLEFRS YTQLSEKLAS SSSYTAPPLN EDGPKGVASA 

        70         80         90        100        110        120 
VSQGSESVVS WTTLTHVYSI LGAYGGPTCL YPTATYFLMG TSKGCVLIFN YNEHLQTILV 

       130        140        150        160        170        180 
PTLSEDPSIH SIRSPVKSIV ICSDGTHVAA SYETGNICIW NLNVGYRVKP TSEPTNGMTP 

       190        200        210        220        230        240 
TPALPAVLHI DDHVNKEITG LDFFGARHTA LIVSDRTGKV SLYNGYRRGF WQLVYNSKKI 

       250        260        270        280        290        300 
LDVNSSKEKL IRSKLSPLIS REKISTNLLS VLTTTHFALI LLSPHVSLMF QETVEPSVQN 

       310        320        330        340        350        360 
SLVVNSSISW TQNCSRVAYS VNNKISVISI SSSDFNVQSA SHSPEFAESI LSIQWIDQLL 

       370        380        390        400        410        420 
LGVLTISHQF LVLHPQHDFK ILLRLDFLIH DLMIPPNKYF VISRRSFYLL TNYSFKIGKF 

       430        440        450        460        470        480 
VSWSDITLRH ILKGDYLGAL EFIESLLQPY CPLANLLKLD NNTEERTKQL MEPFYNLSLA 

       490        500        510        520        530        540 
ALRFLIKKDN ADYNRVYQLL MVVVRVLQQS SKKLDSIPSL DVFLEQGLEF FELKDNAVYF 

       550        560        570        580        590        600 
EVVANIVAQG SVTSISPVLF RSIIDYYAKE ENLKVIEDLI IMLNPTTLDV DLAVKLCQKY 

       610        620        630        640        650        660 
NLFDLLIYIW NKIFDDYQTP VVDLIYRISN QSEKCVIFNG PQVPPETTIF DYVTYILTGR 

       670        680        690        700        710        720 
QYPQNLSISP SDKCSKIQRE LSAFIFSGFS IKWPSNSNHK LYICENPEEE PAFPYFHLLL 

       730        740        750        760        770        780 
KSNPSRFLAM LNEVFEASLF NDDNDMVASV GEAELVSRQY VIDLLLDAMK DTGNSDNIRV 

       790        800        810        820        830        840 
LVAIFIATSI SKYPQFIKVS NQALDCVVNT ICSSRVQGIY EISQIALESL LPYYHSRTTE 

       850        860        870        880        890        900 
NFILELKEKN FNKVLFHIYK SENKYASALS LILETKDIEK EYNTDIVSIT DYILKKCPPG 

       910        920        930        940        950        960 
SLECGKVTEV IETNFDLLLS RIGIEKCVTI FSDFDYNLHQ EILEVKNEET QQKYLDKLFS 

       970        980        990       1000       1010       1020 
TPNINNKVDK RLRNLHIELN CKYKSKREMI LWLNGTVLSN AESLQILDLL NQDSNFEAAA 

      1030       1040       1050       1060       1070       1080 
IIHERLESFN LAVRDLLSFI EQCLNEGKTN ISTLLESLRR AFDDCNSAGT EKKSCWILLI 

      1090       1100       1110       1120       1130       1140 
TFLITLYGKY PSHDERKDLC NKLLQEAFLG LVRSKSSSQK DSGGEFWEIM SSVLEHQDVI 

      1150       1160       1170       1180       1190       1200 
LMKVQDLKQL LLNVFNTYKL ERSLSELIQK IIEDSSQDLV QQYRKFLSEG WSIHTDDCEI 

      1210       1220       1230       1240       1250       1260 
CGKKIWGAGL DPLLFLAWEN VQRHQDMISV DLKTPLVIFK CHHGFHQTCL ENLAQKPDEY 

      1270 
SCLICQTESN PKIV 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Fisk D., Cherry J.M.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The VPS8 gene is required for localization and trafficking of the CPY sorting receptor in Saccharomyces cerevisiae."
Chen Y.-J., Stevens T.H.
Eur. J. Cell Biol. 70:289-297(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 99-1274.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L22015 Genomic DNA. Translation: AAC04955.2.
U44026 Genomic DNA. Translation: AAB49810.1.
BK006935 Genomic DNA. Translation: DAA06984.1.
PIRS40899.
RefSeqNP_009399.2. NM_001178149.1.

3D structure databases

ProteinModelPortalP39702.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31788. 332 interactions.
DIPDIP-6298N.
IntActP39702. 8 interactions.
MINTMINT-615280.
STRING4932.YAL002W.

Proteomic databases

MaxQBP39702.
PaxDbP39702.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAL002W; YAL002W; YAL002W.
GeneID851261.
KEGGsce:YAL002W.

Organism-specific databases

CYGDYAL002w.
SGDS000000002. VPS8.

Phylogenomic databases

eggNOGNOG327467.
GeneTreeENSGT00390000010672.
HOGENOMHOG000154829.
OMATISHQFL.
OrthoDBEOG79KPPM.

Enzyme and pathway databases

BioCycYEAST:G3O-28817-MONOMER.

Gene expression databases

GenevestigatorP39702.

Family and domain databases

Gene3D2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProIPR000547. Clathrin_H-chain/VPS_repeat.
IPR025941. Vps8_central_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamPF12816. Vps8. 1 hit.
[Graphical view]
SMARTSM00184. RING. 1 hit.
SM00320. WD40. 2 hits.
[Graphical view]
SUPFAMSSF50978. SSF50978. 2 hits.
PROSITEPS50236. CHCR. 2 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968219.

Entry information

Entry nameVPS8_YEAST
AccessionPrimary (citable) accession number: P39702
Secondary accession number(s): D6VPL4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 19, 2004
Last modified: June 11, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families