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P39702

- VPS8_YEAST

UniProt

P39702 - VPS8_YEAST

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Protein

Vacuolar protein sorting-associated protein 8

Gene

VPS8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Required for localization and recycling of the CPY sorting receptor (VPS10) to the late-Golgi compartment. Involved in the retention of proteins to the late-Golgi. Plays an integral role in the complex vacuolar protein sorting process.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1198 – 126669RING-type; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. GTPase binding Source: SGD
  2. protein anchor Source: SGD
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
  2. protein targeting to vacuole Source: SGD
  3. vesicle-mediated transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-28817-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 8
Alternative name(s):
Vacuolar protein-targeting protein 8
Gene namesi
Name:VPS8
Synonyms:VPT8
Ordered Locus Names:YAL002W
ORF Names:FUN15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL002w.
SGDiS000000002. VPS8.

Subcellular locationi

Golgi apparatusGolgi stack
Note: Associated with the late-Golgi membranes.

GO - Cellular componenti

  1. CORVET complex Source: SGD
  2. Golgi apparatus Source: UniProtKB-KW
  3. late endosome Source: SGD
  4. membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12741274Vacuolar protein sorting-associated protein 8PRO_0000055901Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP39702.
PaxDbiP39702.

Expressioni

Gene expression databases

GenevestigatoriP39702.

Interactioni

Protein-protein interaction databases

BioGridi31788. 335 interactions.
DIPiDIP-6298N.
IntActiP39702. 8 interactions.
MINTiMINT-615280.
STRINGi4932.YAL002W.

Structurei

3D structure databases

ProteinModelPortaliP39702.
SMRiP39702. Positions 134-165.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati75 – 11945WD 1Add
BLAST
Repeati131 – 17040WD 2Add
BLAST
Repeati193 – 23341WD 3Add
BLAST
Repeati507 – 665159CHCR 1Add
BLAST
Repeati915 – 1092178CHCR 2Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi40 – 434Poly-Ser

Sequence similaritiesi

Belongs to the VPS8 family.Curated
Contains 2 CHCR (clathrin heavy-chain) repeats.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
Contains 3 WD repeats.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri1198 – 126669RING-type; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, WD repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG327467.
GeneTreeiENSGT00390000010672.
HOGENOMiHOG000154829.
InParanoidiP39702.
OMAiTISHQFL.
OrthoDBiEOG79KPPM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR000547. Clathrin_H-chain/VPS_repeat.
IPR025941. Vps8_central_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF12816. Vps8. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
SM00320. WD40. 2 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
PROSITEiPS50236. CHCR. 2 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39702 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEQNGLDHDS RSSIDTTIND TQKTFLEFRS YTQLSEKLAS SSSYTAPPLN
60 70 80 90 100
EDGPKGVASA VSQGSESVVS WTTLTHVYSI LGAYGGPTCL YPTATYFLMG
110 120 130 140 150
TSKGCVLIFN YNEHLQTILV PTLSEDPSIH SIRSPVKSIV ICSDGTHVAA
160 170 180 190 200
SYETGNICIW NLNVGYRVKP TSEPTNGMTP TPALPAVLHI DDHVNKEITG
210 220 230 240 250
LDFFGARHTA LIVSDRTGKV SLYNGYRRGF WQLVYNSKKI LDVNSSKEKL
260 270 280 290 300
IRSKLSPLIS REKISTNLLS VLTTTHFALI LLSPHVSLMF QETVEPSVQN
310 320 330 340 350
SLVVNSSISW TQNCSRVAYS VNNKISVISI SSSDFNVQSA SHSPEFAESI
360 370 380 390 400
LSIQWIDQLL LGVLTISHQF LVLHPQHDFK ILLRLDFLIH DLMIPPNKYF
410 420 430 440 450
VISRRSFYLL TNYSFKIGKF VSWSDITLRH ILKGDYLGAL EFIESLLQPY
460 470 480 490 500
CPLANLLKLD NNTEERTKQL MEPFYNLSLA ALRFLIKKDN ADYNRVYQLL
510 520 530 540 550
MVVVRVLQQS SKKLDSIPSL DVFLEQGLEF FELKDNAVYF EVVANIVAQG
560 570 580 590 600
SVTSISPVLF RSIIDYYAKE ENLKVIEDLI IMLNPTTLDV DLAVKLCQKY
610 620 630 640 650
NLFDLLIYIW NKIFDDYQTP VVDLIYRISN QSEKCVIFNG PQVPPETTIF
660 670 680 690 700
DYVTYILTGR QYPQNLSISP SDKCSKIQRE LSAFIFSGFS IKWPSNSNHK
710 720 730 740 750
LYICENPEEE PAFPYFHLLL KSNPSRFLAM LNEVFEASLF NDDNDMVASV
760 770 780 790 800
GEAELVSRQY VIDLLLDAMK DTGNSDNIRV LVAIFIATSI SKYPQFIKVS
810 820 830 840 850
NQALDCVVNT ICSSRVQGIY EISQIALESL LPYYHSRTTE NFILELKEKN
860 870 880 890 900
FNKVLFHIYK SENKYASALS LILETKDIEK EYNTDIVSIT DYILKKCPPG
910 920 930 940 950
SLECGKVTEV IETNFDLLLS RIGIEKCVTI FSDFDYNLHQ EILEVKNEET
960 970 980 990 1000
QQKYLDKLFS TPNINNKVDK RLRNLHIELN CKYKSKREMI LWLNGTVLSN
1010 1020 1030 1040 1050
AESLQILDLL NQDSNFEAAA IIHERLESFN LAVRDLLSFI EQCLNEGKTN
1060 1070 1080 1090 1100
ISTLLESLRR AFDDCNSAGT EKKSCWILLI TFLITLYGKY PSHDERKDLC
1110 1120 1130 1140 1150
NKLLQEAFLG LVRSKSSSQK DSGGEFWEIM SSVLEHQDVI LMKVQDLKQL
1160 1170 1180 1190 1200
LLNVFNTYKL ERSLSELIQK IIEDSSQDLV QQYRKFLSEG WSIHTDDCEI
1210 1220 1230 1240 1250
CGKKIWGAGL DPLLFLAWEN VQRHQDMISV DLKTPLVIFK CHHGFHQTCL
1260 1270
ENLAQKPDEY SCLICQTESN PKIV
Length:1,274
Mass (Da):144,962
Last modified:July 19, 2004 - v2
Checksum:iE4476C7C64BFF2CE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L22015 Genomic DNA. Translation: AAC04955.2.
U44026 Genomic DNA. Translation: AAB49810.1.
BK006935 Genomic DNA. Translation: DAA06984.1.
PIRiS40899.
RefSeqiNP_009399.2. NM_001178149.1.

Genome annotation databases

EnsemblFungiiYAL002W; YAL002W; YAL002W.
GeneIDi851261.
KEGGisce:YAL002W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L22015 Genomic DNA. Translation: AAC04955.2 .
U44026 Genomic DNA. Translation: AAB49810.1 .
BK006935 Genomic DNA. Translation: DAA06984.1 .
PIRi S40899.
RefSeqi NP_009399.2. NM_001178149.1.

3D structure databases

ProteinModelPortali P39702.
SMRi P39702. Positions 134-165.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31788. 335 interactions.
DIPi DIP-6298N.
IntActi P39702. 8 interactions.
MINTi MINT-615280.
STRINGi 4932.YAL002W.

Proteomic databases

MaxQBi P39702.
PaxDbi P39702.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL002W ; YAL002W ; YAL002W .
GeneIDi 851261.
KEGGi sce:YAL002W.

Organism-specific databases

CYGDi YAL002w.
SGDi S000000002. VPS8.

Phylogenomic databases

eggNOGi NOG327467.
GeneTreei ENSGT00390000010672.
HOGENOMi HOG000154829.
InParanoidi P39702.
OMAi TISHQFL.
OrthoDBi EOG79KPPM.

Enzyme and pathway databases

BioCyci YEAST:G3O-28817-MONOMER.

Miscellaneous databases

NextBioi 968219.

Gene expression databases

Genevestigatori P39702.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProi IPR000547. Clathrin_H-chain/VPS_repeat.
IPR025941. Vps8_central_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
Pfami PF12816. Vps8. 1 hit.
[Graphical view ]
SMARTi SM00184. RING. 1 hit.
SM00320. WD40. 2 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 2 hits.
PROSITEi PS50236. CHCR. 2 hits.
PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
    Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
    Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Fisk D., Cherry J.M.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The VPS8 gene is required for localization and trafficking of the CPY sorting receptor in Saccharomyces cerevisiae."
    Chen Y.-J., Stevens T.H.
    Eur. J. Cell Biol. 70:289-297(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 99-1274.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiVPS8_YEAST
AccessioniPrimary (citable) accession number: P39702
Secondary accession number(s): D6VPL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 19, 2004
Last modified: October 29, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3