Vacuolar protein sorting-associated protein 8 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P39702 (VPS8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 8

Alternative name(s):
Vacuolar protein-targeting protein 8

Gene names

Name:	VPS8
Synonyms:	VPT8
Ordered Locus Names:	YAL002W
ORF Names:	FUN15

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1274 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for localization and recycling of the CPY sorting receptor (VPS10) to the late-Golgi compartment. Involved in the retention of proteins to the late-Golgi. Plays an integral role in the complex vacuolar protein sorting process.
Subcellular location	Golgi apparatus › Golgi stack. Note: Associated with the late-Golgi membranes.
Miscellaneous	Present with 736 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the VPS8 family. Contains 1 RING-type zinc finger. Contains 3 WD repeats.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus
Domain	Repeat WD repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	late endosome to vacuole transport via multivesicular body sorting pathway Inferred from mutant phenotype PubMed 19828734. Source: SGD protein targeting to vacuole Inferred from mutant phenotype PubMed 20173035. Source: SGD
Cellular_component	CORVET complex Inferred from direct assay PubMed 17488625. Source: SGD Golgi stack Inferred from electronic annotation. Source: UniProtKB-SubCell late endosome Inferred from direct assay PubMed 19828734. Source: SGD membrane Inferred from direct assay PubMed 8969229. Source: SGD
Molecular_function	GTPase binding Inferred from direct assay PubMed 19828734. Source: SGD protein anchor Inferred from mutant phenotype PubMed 19828734. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1274

1274

Vacuolar protein sorting-associated protein 8

PRO_0000055901

Regions

Repeat

75 – 119

WD 1

Repeat

131 – 170

WD 2

Repeat

193 – 233

WD 3

Zinc finger

1198 – 1266

RING-type; atypical

Compositional bias

40 – 43

Poly-Ser

Amino acid modifications

Modified residue

662

Phosphotyrosine Ref.7

Sequences

Sequence

Length

Mass (Da)

Tools

P39702 [UniParc].

Last modified July 19, 2004. Version 2.
Checksum: E4476C7C64BFF2CE
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FASTA

1,274

144,962

        10         20         30         40         50         60 
MEQNGLDHDS RSSIDTTIND TQKTFLEFRS YTQLSEKLAS SSSYTAPPLN EDGPKGVASA 

        70         80         90        100        110        120 
VSQGSESVVS WTTLTHVYSI LGAYGGPTCL YPTATYFLMG TSKGCVLIFN YNEHLQTILV 

       130        140        150        160        170        180 
PTLSEDPSIH SIRSPVKSIV ICSDGTHVAA SYETGNICIW NLNVGYRVKP TSEPTNGMTP 

       190        200        210        220        230        240 
TPALPAVLHI DDHVNKEITG LDFFGARHTA LIVSDRTGKV SLYNGYRRGF WQLVYNSKKI 

       250        260        270        280        290        300 
LDVNSSKEKL IRSKLSPLIS REKISTNLLS VLTTTHFALI LLSPHVSLMF QETVEPSVQN 

       310        320        330        340        350        360 
SLVVNSSISW TQNCSRVAYS VNNKISVISI SSSDFNVQSA SHSPEFAESI LSIQWIDQLL 

       370        380        390        400        410        420 
LGVLTISHQF LVLHPQHDFK ILLRLDFLIH DLMIPPNKYF VISRRSFYLL TNYSFKIGKF 

       430        440        450        460        470        480 
VSWSDITLRH ILKGDYLGAL EFIESLLQPY CPLANLLKLD NNTEERTKQL MEPFYNLSLA 

       490        500        510        520        530        540 
ALRFLIKKDN ADYNRVYQLL MVVVRVLQQS SKKLDSIPSL DVFLEQGLEF FELKDNAVYF 

       550        560        570        580        590        600 
EVVANIVAQG SVTSISPVLF RSIIDYYAKE ENLKVIEDLI IMLNPTTLDV DLAVKLCQKY 

       610        620        630        640        650        660 
NLFDLLIYIW NKIFDDYQTP VVDLIYRISN QSEKCVIFNG PQVPPETTIF DYVTYILTGR 

       670        680        690        700        710        720 
QYPQNLSISP SDKCSKIQRE LSAFIFSGFS IKWPSNSNHK LYICENPEEE PAFPYFHLLL 

       730        740        750        760        770        780 
KSNPSRFLAM LNEVFEASLF NDDNDMVASV GEAELVSRQY VIDLLLDAMK DTGNSDNIRV 

       790        800        810        820        830        840 
LVAIFIATSI SKYPQFIKVS NQALDCVVNT ICSSRVQGIY EISQIALESL LPYYHSRTTE 

       850        860        870        880        890        900 
NFILELKEKN FNKVLFHIYK SENKYASALS LILETKDIEK EYNTDIVSIT DYILKKCPPG 

       910        920        930        940        950        960 
SLECGKVTEV IETNFDLLLS RIGIEKCVTI FSDFDYNLHQ EILEVKNEET QQKYLDKLFS 

       970        980        990       1000       1010       1020 
TPNINNKVDK RLRNLHIELN CKYKSKREMI LWLNGTVLSN AESLQILDLL NQDSNFEAAA 

      1030       1040       1050       1060       1070       1080 
IIHERLESFN LAVRDLLSFI EQCLNEGKTN ISTLLESLRR AFDDCNSAGT EKKSCWILLI 

      1090       1100       1110       1120       1130       1140 
TFLITLYGKY PSHDERKDLC NKLLQEAFLG LVRSKSSSQK DSGGEFWEIM SSVLEHQDVI 

      1150       1160       1170       1180       1190       1200 
LMKVQDLKQL LLNVFNTYKL ERSLSELIQK IIEDSSQDLV QQYRKFLSEG WSIHTDDCEI 

      1210       1220       1230       1240       1250       1260 
CGKKIWGAGL DPLLFLAWEN VQRHQDMISV DLKTPLVIFK CHHGFHQTCL ENLAQKPDEY 

      1270 
SCLICQTESN PKIV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region." Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H. Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Fisk D., Cherry J.M. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The VPS8 gene is required for localization and trafficking of the CPY sorting receptor in Saccharomyces cerevisiae." Chen Y.-J., Stevens T.H. Eur. J. Cell Biol. 70:289-297(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 99-1274.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-662, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L22015 Genomic DNA. Translation: AAC04955.2. U44026 Genomic DNA. Translation: AAB49810.1. BK006935 Genomic DNA. Translation: DAA06984.1.
PIR	S40899.
RefSeq	NP_009399.2. NM_001178149.1.
3D structure databases
ProteinModelPortal	P39702.
SMR	P39702. Positions 134-165.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6298N.
IntAct	P39702. 9 interactions.
MINT	MINT-615280.
STRING	4932.YAL002W.
Proteomic databases
PaxDb	P39702.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL002W; YAL002W; YAL002W.
GeneID	851261.
KEGG	sce:YAL002W.
Organism-specific databases
CYGD	YAL002w.
SGD	S000000002. VPS8.
Phylogenomic databases
eggNOG	NOG327467.
GeneTree	ENSGT00390000010672.
HOGENOM	HOG000154829.
OMA	SDCELAL.
OrthoDB	EOG4TMV9D.
Gene expression databases
Genevestigator	P39702.
GermOnline	YAL002W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.130.10.10. 1 hit. 3.30.40.10. 1 hit.
InterPro	IPR025941. Vps8_central_dom. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR017986. WD40_repeat_dom. IPR001841. Znf_RING. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF12816. Vps8. 1 hit. [Graphical view]
SMART	SM00184. RING. 1 hit. SM00320. WD40. 2 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. False negative. PS50294. WD_REPEATS_REGION. False negative. PS00518. ZF_RING_1. False negative. PS50089. ZF_RING_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968219.

Entry information

Entry name

VPS8_YEAST

Accession

Primary (citable) accession number: P39702
Secondary accession number(s): D6VPL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents