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P39702

- VPS8_YEAST

UniProt

P39702 - VPS8_YEAST

Protein

Vacuolar protein sorting-associated protein 8

Gene

VPS8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Required for localization and recycling of the CPY sorting receptor (VPS10) to the late-Golgi compartment. Involved in the retention of proteins to the late-Golgi. Plays an integral role in the complex vacuolar protein sorting process.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1198 – 126669RING-type; atypicalPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. GTPase binding Source: SGD
    2. protein anchor Source: SGD
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
    2. protein targeting to vacuole Source: SGD
    3. vesicle-mediated transport Source: InterPro

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28817-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar protein sorting-associated protein 8
    Alternative name(s):
    Vacuolar protein-targeting protein 8
    Gene namesi
    Name:VPS8
    Synonyms:VPT8
    Ordered Locus Names:YAL002W
    ORF Names:FUN15
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAL002w.
    SGDiS000000002. VPS8.

    Subcellular locationi

    Golgi apparatusGolgi stack
    Note: Associated with the late-Golgi membranes.

    GO - Cellular componenti

    1. CORVET complex Source: SGD
    2. Golgi stack Source: UniProtKB-SubCell
    3. late endosome Source: SGD
    4. membrane Source: SGD

    Keywords - Cellular componenti

    Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12741274Vacuolar protein sorting-associated protein 8PRO_0000055901Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP39702.
    PaxDbiP39702.

    Expressioni

    Gene expression databases

    GenevestigatoriP39702.

    Interactioni

    Protein-protein interaction databases

    BioGridi31788. 335 interactions.
    DIPiDIP-6298N.
    IntActiP39702. 8 interactions.
    MINTiMINT-615280.
    STRINGi4932.YAL002W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39702.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati75 – 11945WD 1Add
    BLAST
    Repeati131 – 17040WD 2Add
    BLAST
    Repeati193 – 23341WD 3Add
    BLAST
    Repeati507 – 665159CHCR 1Add
    BLAST
    Repeati915 – 1092178CHCR 2Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi40 – 434Poly-Ser

    Sequence similaritiesi

    Belongs to the VPS8 family.Curated
    Contains 2 CHCR (clathrin heavy-chain) repeats.PROSITE-ProRule annotation
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation
    Contains 3 WD repeats.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri1198 – 126669RING-type; atypicalPROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, WD repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG327467.
    GeneTreeiENSGT00390000010672.
    HOGENOMiHOG000154829.
    OMAiTISHQFL.
    OrthoDBiEOG79KPPM.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    3.30.40.10. 1 hit.
    InterProiIPR000547. Clathrin_H-chain/VPS_repeat.
    IPR025941. Vps8_central_dom.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF12816. Vps8. 1 hit.
    [Graphical view]
    SMARTiSM00184. RING. 1 hit.
    SM00320. WD40. 2 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 2 hits.
    PROSITEiPS50236. CHCR. 2 hits.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39702-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQNGLDHDS RSSIDTTIND TQKTFLEFRS YTQLSEKLAS SSSYTAPPLN     50
    EDGPKGVASA VSQGSESVVS WTTLTHVYSI LGAYGGPTCL YPTATYFLMG 100
    TSKGCVLIFN YNEHLQTILV PTLSEDPSIH SIRSPVKSIV ICSDGTHVAA 150
    SYETGNICIW NLNVGYRVKP TSEPTNGMTP TPALPAVLHI DDHVNKEITG 200
    LDFFGARHTA LIVSDRTGKV SLYNGYRRGF WQLVYNSKKI LDVNSSKEKL 250
    IRSKLSPLIS REKISTNLLS VLTTTHFALI LLSPHVSLMF QETVEPSVQN 300
    SLVVNSSISW TQNCSRVAYS VNNKISVISI SSSDFNVQSA SHSPEFAESI 350
    LSIQWIDQLL LGVLTISHQF LVLHPQHDFK ILLRLDFLIH DLMIPPNKYF 400
    VISRRSFYLL TNYSFKIGKF VSWSDITLRH ILKGDYLGAL EFIESLLQPY 450
    CPLANLLKLD NNTEERTKQL MEPFYNLSLA ALRFLIKKDN ADYNRVYQLL 500
    MVVVRVLQQS SKKLDSIPSL DVFLEQGLEF FELKDNAVYF EVVANIVAQG 550
    SVTSISPVLF RSIIDYYAKE ENLKVIEDLI IMLNPTTLDV DLAVKLCQKY 600
    NLFDLLIYIW NKIFDDYQTP VVDLIYRISN QSEKCVIFNG PQVPPETTIF 650
    DYVTYILTGR QYPQNLSISP SDKCSKIQRE LSAFIFSGFS IKWPSNSNHK 700
    LYICENPEEE PAFPYFHLLL KSNPSRFLAM LNEVFEASLF NDDNDMVASV 750
    GEAELVSRQY VIDLLLDAMK DTGNSDNIRV LVAIFIATSI SKYPQFIKVS 800
    NQALDCVVNT ICSSRVQGIY EISQIALESL LPYYHSRTTE NFILELKEKN 850
    FNKVLFHIYK SENKYASALS LILETKDIEK EYNTDIVSIT DYILKKCPPG 900
    SLECGKVTEV IETNFDLLLS RIGIEKCVTI FSDFDYNLHQ EILEVKNEET 950
    QQKYLDKLFS TPNINNKVDK RLRNLHIELN CKYKSKREMI LWLNGTVLSN 1000
    AESLQILDLL NQDSNFEAAA IIHERLESFN LAVRDLLSFI EQCLNEGKTN 1050
    ISTLLESLRR AFDDCNSAGT EKKSCWILLI TFLITLYGKY PSHDERKDLC 1100
    NKLLQEAFLG LVRSKSSSQK DSGGEFWEIM SSVLEHQDVI LMKVQDLKQL 1150
    LLNVFNTYKL ERSLSELIQK IIEDSSQDLV QQYRKFLSEG WSIHTDDCEI 1200
    CGKKIWGAGL DPLLFLAWEN VQRHQDMISV DLKTPLVIFK CHHGFHQTCL 1250
    ENLAQKPDEY SCLICQTESN PKIV 1274
    Length:1,274
    Mass (Da):144,962
    Last modified:July 19, 2004 - v2
    Checksum:iE4476C7C64BFF2CE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22015 Genomic DNA. Translation: AAC04955.2.
    U44026 Genomic DNA. Translation: AAB49810.1.
    BK006935 Genomic DNA. Translation: DAA06984.1.
    PIRiS40899.
    RefSeqiNP_009399.2. NM_001178149.1.

    Genome annotation databases

    EnsemblFungiiYAL002W; YAL002W; YAL002W.
    GeneIDi851261.
    KEGGisce:YAL002W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22015 Genomic DNA. Translation: AAC04955.2 .
    U44026 Genomic DNA. Translation: AAB49810.1 .
    BK006935 Genomic DNA. Translation: DAA06984.1 .
    PIRi S40899.
    RefSeqi NP_009399.2. NM_001178149.1.

    3D structure databases

    ProteinModelPortali P39702.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31788. 335 interactions.
    DIPi DIP-6298N.
    IntActi P39702. 8 interactions.
    MINTi MINT-615280.
    STRINGi 4932.YAL002W.

    Proteomic databases

    MaxQBi P39702.
    PaxDbi P39702.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL002W ; YAL002W ; YAL002W .
    GeneIDi 851261.
    KEGGi sce:YAL002W.

    Organism-specific databases

    CYGDi YAL002w.
    SGDi S000000002. VPS8.

    Phylogenomic databases

    eggNOGi NOG327467.
    GeneTreei ENSGT00390000010672.
    HOGENOMi HOG000154829.
    OMAi TISHQFL.
    OrthoDBi EOG79KPPM.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28817-MONOMER.

    Miscellaneous databases

    NextBioi 968219.

    Gene expression databases

    Genevestigatori P39702.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    3.30.40.10. 1 hit.
    InterProi IPR000547. Clathrin_H-chain/VPS_repeat.
    IPR025941. Vps8_central_dom.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR017986. WD40_repeat_dom.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF12816. Vps8. 1 hit.
    [Graphical view ]
    SMARTi SM00184. RING. 1 hit.
    SM00320. WD40. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 2 hits.
    PROSITEi PS50236. CHCR. 2 hits.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
      Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
      Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Fisk D., Cherry J.M.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The VPS8 gene is required for localization and trafficking of the CPY sorting receptor in Saccharomyces cerevisiae."
      Chen Y.-J., Stevens T.H.
      Eur. J. Cell Biol. 70:289-297(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 99-1274.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiVPS8_YEAST
    AccessioniPrimary (citable) accession number: P39702
    Secondary accession number(s): D6VPL4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 736 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3