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Protein

Sulfite reductase [NADPH] flavoprotein component

Gene

MET10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route).
Proteins known to be involved in this subpathway in this organism are:
  1. Sulfite reductase [NADPH] flavoprotein component (MET10), Sulfite reductase [NADPH] subunit beta (MET5)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route), the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi684 – 69512FADBy similarityAdd
BLAST
Nucleotide bindingi814 – 82411FAD (flavin part)By similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cysteine biosynthetic process Source: UniProtKB-KW
  • hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
  • methionine biosynthetic process Source: UniProtKB-KW
  • sulfate assimilation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Electron transport, Methionine biosynthesis, Transport

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-385.
YEAST:MONOMER3O-21.
UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] flavoprotein component (EC:1.8.1.2)
Gene namesi
Name:MET10
Ordered Locus Names:YFR030W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR030W.
SGDiS000001926. MET10.

Subcellular locationi

GO - Cellular componenti

  • sulfite reductase complex (NADPH) Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10351035Sulfite reductase [NADPH] flavoprotein componentPRO_0000199947Add
BLAST

Proteomic databases

MaxQBiP39692.
PRIDEiP39692.

Expressioni

Inductioni

By methionine deprivation.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi31185. 41 interactions.
DIPiDIP-6609N.
IntActiP39692. 10 interactions.
MINTiMINT-698633.

Structurei

3D structure databases

ProteinModelPortaliP39692.
SMRiP39692. Positions 225-545, 639-1035.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini648 – 879232FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000216012.
InParanoidiP39692.
KOiK00380.
OMAiIITHIGA.
OrthoDBiEOG092C1RRH.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.920. 1 hit.
3.40.920.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR019752. Pyrv/ketoisovalerate_OxRed_cat.
IPR002869. Pyrv_flavodox_OxRed_cen.
IPR017938. Riboflavin_synthase-like_b-brl.
IPR009014. Transketo_C/PFOR_II.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00371. FPNCR.
SUPFAMiSSF52922. SSF52922. 1 hit.
SSF53323. SSF53323. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39692-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVEFATNPF GEAKNATSLP KYGTPVTAIS SVLFNNVDSI FAYKSFSQPD
60 70 80 90 100
LLHQDLKKWS EKRGNESRGK PFFQELDIRS GAGLAPLGFS HGLKNTTAIV
110 120 130 140 150
APGFSLPYFI NSLKTVSHDG KFLLNVGALN YDNATGSVTN DYVTALDAAS
160 170 180 190 200
KLKYGVVTPI SANEVQSVAL LALAIATFSN NSGAINLFDG LNYSKTVLPL
210 220 230 240 250
VESVPEASIL AKLSKVIAPD AAFDDVLDKF NELTGLRLHN FQYFGAQDAE
260 270 280 290 300
TVFITYGSLE SELFNSAISG NNSKIGLINV RVPLPFNVAK FVTHVPSTTK
310 320 330 340 350
QIVVIGQTLD GSSPSFLRSQ VSAALFYHGR TSISVSEYIY QPDFIWSPKA
360 370 380 390 400
VKSIVSSFIP EFTYNADSSF GEGFIYWASD KSINIDVASK LVKALSLEDG
410 420 430 440 450
KFVSLRTKFD NLANAGTFQA QFVTSKEQIP VSNIDSTKLS VVEDVSLLKH
460 470 480 490 500
LDVAATVAEQ GSIALVSQKA VKDLDLNSVE SYVKNLGIPE SFLISIAKKN
510 520 530 540 550
IKLFIIDGET TNDESKLSLF IQAVFWKLAF GLDVAECTNR IWKSIDSGAD
560 570 580 590 600
ISAASISEFL TGAFKNFLSE VPLALYTKFS EINIEKKEDE EEPAALPIFV
610 620 630 640 650
NETSFLPNNS TIEEIPLPET SEISDIAKKL SFKEAYEVEN KLRPDLPVKN
660 670 680 690 700
FVVKVKENRR VTPADYDRYI FHIEFDISGT GMTYDIGEAL GIHARNNESL
710 720 730 740 750
VKEFLTFYGL NESDVVLVPN KDNHHLLETR TVLQAFVENL DIFGKPPKRF
760 770 780 790 800
YESLIPYASN EEEKKKLEDL VTPAGAVDLK RFQDVEYYTY ADIFELFPSV
810 820 830 840 850
RPSLEELVTI IEPLKRREYS IASSQKVHPN EVHLLIVVVD WVDNKGRKRY
860 870 880 890 900
GQASKYISDL AVGSELVVSV KPSVMKLPPS PKQPVIMSGL GTGLAPFKAI
910 920 930 940 950
VEEKLWQKQQ GYEIGEVFLY LGSRHKREEY LYGELWEAYK DAGIITHIGA
960 970 980 990 1000
AFSRDQPQKI YIQDRIKENL DELKTAMIDN KGSFYLCGPT WPVPDITQAL
1010 1020 1030
QDILAKDAEE RGIKVDLDAA IEELKEASRY ILEVY
Length:1,035
Mass (Da):114,829
Last modified:February 15, 2005 - v2
Checksum:i924B4047D645E1C3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti172 – 1721A → T in BAA08076 (PubMed:7788524).Curated
Sequence conflicti420 – 4201A → G in AAA61982 (PubMed:7928966).Curated
Sequence conflicti977 – 9771M → V in AAA61982 (PubMed:7928966).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26503 Genomic DNA. Translation: AAA61982.1.
D44610 Genomic DNA. Translation: BAA08076.1.
D50617 Genomic DNA. Translation: BAA09269.1.
U05820 Genomic DNA. Translation: AAA17417.2.
BK006940 Genomic DNA. Translation: DAA12470.1.
PIRiS56285.
RefSeqiNP_116686.3. NM_001179995.3.

Genome annotation databases

EnsemblFungiiBAA09269; BAA09269; BAA09269.
YFR030W; YFR030W; YFR030W.
GeneIDi850588.
KEGGisce:YFR030W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26503 Genomic DNA. Translation: AAA61982.1.
D44610 Genomic DNA. Translation: BAA08076.1.
D50617 Genomic DNA. Translation: BAA09269.1.
U05820 Genomic DNA. Translation: AAA17417.2.
BK006940 Genomic DNA. Translation: DAA12470.1.
PIRiS56285.
RefSeqiNP_116686.3. NM_001179995.3.

3D structure databases

ProteinModelPortaliP39692.
SMRiP39692. Positions 225-545, 639-1035.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31185. 41 interactions.
DIPiDIP-6609N.
IntActiP39692. 10 interactions.
MINTiMINT-698633.

Proteomic databases

MaxQBiP39692.
PRIDEiP39692.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09269; BAA09269; BAA09269.
YFR030W; YFR030W; YFR030W.
GeneIDi850588.
KEGGisce:YFR030W.

Organism-specific databases

EuPathDBiFungiDB:YFR030W.
SGDiS000001926. MET10.

Phylogenomic databases

HOGENOMiHOG000216012.
InParanoidiP39692.
KOiK00380.
OMAiIITHIGA.
OrthoDBiEOG092C1RRH.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00207.
BioCyciMetaCyc:MONOMER-385.
YEAST:MONOMER3O-21.

Miscellaneous databases

PROiP39692.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.920. 1 hit.
3.40.920.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR019752. Pyrv/ketoisovalerate_OxRed_cat.
IPR002869. Pyrv_flavodox_OxRed_cen.
IPR017938. Riboflavin_synthase-like_b-brl.
IPR009014. Transketo_C/PFOR_II.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00371. FPNCR.
SUPFAMiSSF52922. SSF52922. 1 hit.
SSF53323. SSF53323. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMET10_YEAST
AccessioniPrimary (citable) accession number: P39692
Secondary accession number(s): D6VTR0, Q12683
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 15, 2005
Last modified: September 7, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1580 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.