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Protein

Sulfite reductase [NADPH] flavoprotein component

Gene

MET10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate.

Miscellaneous

Present with 1580 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route).
Proteins known to be involved in this subpathway in this organism are:
  1. Sulfite reductase [NADPH] flavoprotein component (MET10), Sulfite reductase [NADPH] subunit beta (MET5)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes hydrogen sulfide from sulfite (NADPH route), the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi684 – 695FADBy similarityAdd BLAST12
Nucleotide bindingi814 – 824FAD (flavin part)By similarityAdd BLAST11

GO - Molecular functioni

GO - Biological processi

  • cysteine biosynthetic process Source: UniProtKB-KW
  • hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
  • methionine biosynthetic process Source: UniProtKB-KW
  • sulfate assimilation Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis, Electron transport, Methionine biosynthesis, Transport
LigandFAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER3O-21
YEAST:MONOMER3O-21
UniPathwayiUPA00140; UER00207

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] flavoprotein component (EC:1.8.1.2)
Gene namesi
Name:MET10
Ordered Locus Names:YFR030W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR030W
SGDiS000001926 MET10

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001999471 – 1035Sulfite reductase [NADPH] flavoprotein componentAdd BLAST1035

Proteomic databases

MaxQBiP39692
PaxDbiP39692
PRIDEiP39692

Expressioni

Inductioni

By methionine deprivation.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PRT1P061032EBI-11476,EBI-8973

Protein-protein interaction databases

BioGridi31185, 86 interactors
DIPiDIP-6609N
IntActiP39692, 36 interactors
MINTiP39692
STRINGi4932.YFR030W

Structurei

3D structure databases

ProteinModelPortaliP39692
SMRiP39692
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini648 – 879FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST232

Phylogenomic databases

HOGENOMiHOG000216012
InParanoidiP39692
KOiK00380
OMAiIITHIGA
OrthoDBiEOG092C1RRH

Family and domain databases

Gene3Di1.20.990.10, 1 hit
3.40.50.920, 1 hit
3.40.920.10, 1 hit
InterProiView protein in InterPro
IPR003097 FAD-binding_1
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR001433 OxRdtase_FAD/NAD-bd
IPR002869 Pyrv_flavodox_OxRed_cen
IPR017938 Riboflavin_synthase-like_b-brl
IPR009014 Transketo_C/PFOR_II
PfamiView protein in Pfam
PF00667 FAD_binding_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00371 FPNCR
SUPFAMiSSF52922 SSF52922, 1 hit
SSF53323 SSF53323, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

P39692-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVEFATNPF GEAKNATSLP KYGTPVTAIS SVLFNNVDSI FAYKSFSQPD
60 70 80 90 100
LLHQDLKKWS EKRGNESRGK PFFQELDIRS GAGLAPLGFS HGLKNTTAIV
110 120 130 140 150
APGFSLPYFI NSLKTVSHDG KFLLNVGALN YDNATGSVTN DYVTALDAAS
160 170 180 190 200
KLKYGVVTPI SANEVQSVAL LALAIATFSN NSGAINLFDG LNYSKTVLPL
210 220 230 240 250
VESVPEASIL AKLSKVIAPD AAFDDVLDKF NELTGLRLHN FQYFGAQDAE
260 270 280 290 300
TVFITYGSLE SELFNSAISG NNSKIGLINV RVPLPFNVAK FVTHVPSTTK
310 320 330 340 350
QIVVIGQTLD GSSPSFLRSQ VSAALFYHGR TSISVSEYIY QPDFIWSPKA
360 370 380 390 400
VKSIVSSFIP EFTYNADSSF GEGFIYWASD KSINIDVASK LVKALSLEDG
410 420 430 440 450
KFVSLRTKFD NLANAGTFQA QFVTSKEQIP VSNIDSTKLS VVEDVSLLKH
460 470 480 490 500
LDVAATVAEQ GSIALVSQKA VKDLDLNSVE SYVKNLGIPE SFLISIAKKN
510 520 530 540 550
IKLFIIDGET TNDESKLSLF IQAVFWKLAF GLDVAECTNR IWKSIDSGAD
560 570 580 590 600
ISAASISEFL TGAFKNFLSE VPLALYTKFS EINIEKKEDE EEPAALPIFV
610 620 630 640 650
NETSFLPNNS TIEEIPLPET SEISDIAKKL SFKEAYEVEN KLRPDLPVKN
660 670 680 690 700
FVVKVKENRR VTPADYDRYI FHIEFDISGT GMTYDIGEAL GIHARNNESL
710 720 730 740 750
VKEFLTFYGL NESDVVLVPN KDNHHLLETR TVLQAFVENL DIFGKPPKRF
760 770 780 790 800
YESLIPYASN EEEKKKLEDL VTPAGAVDLK RFQDVEYYTY ADIFELFPSV
810 820 830 840 850
RPSLEELVTI IEPLKRREYS IASSQKVHPN EVHLLIVVVD WVDNKGRKRY
860 870 880 890 900
GQASKYISDL AVGSELVVSV KPSVMKLPPS PKQPVIMSGL GTGLAPFKAI
910 920 930 940 950
VEEKLWQKQQ GYEIGEVFLY LGSRHKREEY LYGELWEAYK DAGIITHIGA
960 970 980 990 1000
AFSRDQPQKI YIQDRIKENL DELKTAMIDN KGSFYLCGPT WPVPDITQAL
1010 1020 1030
QDILAKDAEE RGIKVDLDAA IEELKEASRY ILEVY
Length:1,035
Mass (Da):114,829
Last modified:February 15, 2005 - v2
Checksum:i924B4047D645E1C3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti172A → T in BAA08076 (PubMed:7788524).Curated1
Sequence conflicti420A → G in AAA61982 (PubMed:7928966).Curated1
Sequence conflicti977M → V in AAA61982 (PubMed:7928966).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26503 Genomic DNA Translation: AAA61982.1
D44610 Genomic DNA Translation: BAA08076.1
D50617 Genomic DNA Translation: BAA09269.1
U05820 Genomic DNA Translation: AAA17417.2
BK006940 Genomic DNA Translation: DAA12470.1
PIRiS56285
RefSeqiNP_116686.3, NM_001179995.3

Genome annotation databases

EnsemblFungiiBAA09269; BAA09269; BAA09269
YFR030W; YFR030W; YFR030W
GeneIDi850588
KEGGisce:YFR030W

Similar proteinsi

Entry informationi

Entry nameiMET10_YEAST
AccessioniPrimary (citable) accession number: P39692
Secondary accession number(s): D6VTR0, Q12683
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 15, 2005
Last modified: March 28, 2018
This is version 166 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health