Reviewed, UniProtKB/Swiss-Prot P39689 (CDN1A_MOUSE)

Names and origin

Protein names

Recommended name:
    Cyclin-dependent kinase inhibitor 1
Alternative name(s):
    p21
    CDK-interacting protein 1
    Melanoma differentiation-associated protein

Gene names

Name:	Cdkn1a
Synonyms:	Cip1, Waf1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.
Subunit structure	Interacts with MKRN1 By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Induction	By p53, mezerein (antileukemic compound) and interferon beta.
Post-translational modification	Phosphorylation of Thr-140 or Ser-141 impairs binding to PCNA By similarity. Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation By similarity.
Sequence similarities	Belongs to the CDI family.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Protein kinase inhibitor
PTM	Acetylation Phosphoprotein Ubl conjugation
Gene Ontology (GO)
Biological process	cell cycle arrest Inferred from direct assay. Source: MGI negative regulation of apoptosis Inferred from mutant phenotype. Source: MGI negative regulation of cell proliferation Inferred from mutant phenotype. Source: MGI negative regulation of cyclin-dependent protein kinase activity Inferred from direct assay. Source: MGI positive regulation of B cell proliferation Inferred from genetic interaction. Source: MGI positive regulation of programmed cell death Inferred from mutant phenotype. Source: MGI response to DNA damage stimulus Inferred from mutant phenotype. Source: MGI response to UV Inferred from mutant phenotype. Source: MGI
Cellular component	cyclin-dependent protein kinase holoenzyme complex Inferred from physical interaction. Source: MGI cytosol Inferred from direct assay. Source: MGI nucleus Inferred from direct assay. Source: MGI
Molecular function	cyclin binding Inferred from physical interaction. Source: MGI cyclin-dependent protein kinase inhibitor activity Inferred from direct assay. Source: MGI zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Gadd45g	Q9Z111	1	EBI-1174103,EBI-1173616

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 159	158	Cyclin-dependent kinase inhibitor 1		PRO_0000190080
Regions
	Zinc finger	12 – 40	29	C4-type Potential
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	125	1	Phosphoserine By similarity
	Modified residue	140	1	Phosphothreonine By similarity
	Modified residue	141	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	30	1	R → S Ref.4
	Sequence conflict	56 – 57	2	TP → RQ Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P39689-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 37B7C22B9A2FD089	FASTA	159	17,785
	10 20 30 40 50 60 MSNPGDVRPV PHRSKVCRCL FGPVDSEQLR RDCDALMAGC LQEARERWNF DFVTETPLEG 70 80 90 100 110 120 NFVWERVRSL GLPKVYLSPG SRSRDDLGGD KRPSTSSALL QGPAPEDHVA LSLSCTLVSE 130 140 150 RPEDSPGGPG TSQGRKRRQT SLTDFYHSKR RLVFCKRKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, sequencing, chromosomal localization and expression of mouse p21 (Waf1)." Huppi K., Siwarski D., Dosik J., Michieli P., Chedid M., Reed S., Mock B., Givol D., Mushinski J.F. Oncogene 9:3017-3020(1994) [PubMed: 8084607] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BXSB. Tissue: Spleen.
[2]	"Topological control of p21WAF1/CIP1 expression in normal and neoplastic tissues." El-Deiry W.S., Tokino T., Waldman T., Velculescu V.E., Oliner J.D., Burell M., Hill D.E., Rees J.L., Hamilton S.R., Kinzler K.W., Vogelstein B. Cancer Res. 55:2910-2919(1995) [PubMed: 7796420] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Mammary gland.
[4]	"WAF1, a potential mediator of p53 tumor suppression." El-Deiry W.S., Tokino T., Velculescu V.E., Levy D.B., Parsons R., Trent J.M., Lin D., Mercer W.E., Kinzler K.W., Vogelstein B. Cell 75:817-825(1993) [PubMed: 8242752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-143.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U09507 mRNA. Translation: AAB60456.1. U24173 mRNA. Translation: AAC52220.1. BC002043 mRNA. Translation: AAH02043.1.
IPI	IPI00108058.
PIR	A49438. I49023.
RefSeq	NP_001104569.1. NP_031695.1.
UniGene	Mm.195663
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-24178N.
IntAct	P39689. 2 interactions.
STRING	P39689.
PTM databases
PhosphoSite	P39689.
Proteomic databases
PRIDE	P39689.
Genome annotation databases
Ensembl	ENSMUST00000023829; ENSMUSP00000023829; ENSMUSG00000023067; Mus musculus. [Genome view] ENSMUST00000119901; ENSMUSP00000113150; ENSMUSG00000023067; Mus musculus. [Genome view] ENSMUST00000122348; ENSMUSP00000112411; ENSMUSG00000023067; Mus musculus. [Genome view]
GeneID	12575.
KEGG	mmu:12575.
UCSC	uc008bsg.1. mouse.
Organism-specific databases
CTD	12575.
MGI	MGI:104556. Cdkn1a.
Phylogenomic databases
HOGENOM	HBG506854.
HOVERGEN	P39689.
InParanoid	P39689.
OMA	RRDCDAL.
OrthoDB	EOG9WM7DW.
Gene expression databases
ArrayExpress	P39689.
Bgee	P39689.
CleanEx	MM_CDKN1A.
Genevestigator	P39689.
GermOnline	ENSMUSG00000023067. Mus musculus.
Family and domain databases
InterPro	IPR003175. CDI. [Graphical view]
PANTHER	PTHR10265. CDI. 1 hit.
Pfam	PF02234. CDI. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	281686.
SOURCE	Search...

Entry information

Entry name

CDN1A_MOUSE

Accession

Primary (citable) accession number: P39689

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	December 15, 2009
This is version 84 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)