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Protein

Acidic leucine-rich nuclear phosphoprotein 32 family member A

Gene

ANP32A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.4 Publications

GO - Molecular functioni

  • histone binding Source: GO_Central
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000140350-MONOMER.
ReactomeiR-HSA-450520. HuR (ELAVL1) binds and stabilizes mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic leucine-rich nuclear phosphoprotein 32 family member A
Alternative name(s):
Acidic nuclear phosphoprotein pp32
Short name:
pp32
Leucine-rich acidic nuclear protein
Short name:
LANP
Mapmodulin
Potent heat-stable protein phosphatase 2A inhibitor I1PP2A
Putative HLA-DR-associated protein I
Short name:
PHAPI
Gene namesi
Name:ANP32A
Synonyms:C15orf1, LANP, MAPM, PHAP1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:13233. ANP32A.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi8125.
OpenTargetsiENSG00000140350.
PharmGKBiPA24811.

Polymorphism and mutation databases

BioMutaiANP32A.
DMDMi730318.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001375921 – 249Acidic leucine-rich nuclear phosphoprotein 32 family member AAdd BLAST249

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphothreonineCombined sources1
Modified residuei17PhosphoserineCombined sources1
Modified residuei158PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on serine residues.
The N-terminus is blocked.
Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP39687.
MaxQBiP39687.
PaxDbiP39687.
PeptideAtlasiP39687.
PRIDEiP39687.
TopDownProteomicsiP39687.

2D gel databases

SWISS-2DPAGEP39687.

PTM databases

iPTMnetiP39687.
PhosphoSitePlusiP39687.
SwissPalmiP39687.

Miscellaneous databases

PMAP-CutDBP39687.

Expressioni

Tissue specificityi

Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.1 Publication

Gene expression databases

BgeeiENSG00000140350.
CleanExiHS_ANP32A.
ExpressionAtlasiP39687. baseline and differential.
GenevisibleiP39687. HS.

Organism-specific databases

HPAiCAB005231.
HPA063133.
HPA067561.

Interactioni

Subunit structurei

Component of the SET complex, composed of at least ANP32A, APEX1, HMGB2, NME1, SET and TREX1. Directly interacts with SET. Interacts with ATXN1/SCA1. Interacts with MAP1B. Interacts with ELAVL1. Part of the INHAT (inhibitor of histone acetyltransferases) complex. Interacts with E4F1.6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AXIN1O151692EBI-359234,EBI-710484
E4F1Q66K893EBI-359234,EBI-1227043
PPP2CAP677752EBI-359234,EBI-712311
Ppp2caP633312EBI-359234,EBI-7050205From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113791. 55 interactors.
IntActiP39687. 19 interactors.
MINTiMINT-4999627.
STRINGi9606.ENSP00000417864.

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 10Combined sources8
Turni11 – 13Combined sources3
Helixi16 – 18Combined sources3
Beta strandi20 – 23Combined sources4
Beta strandi46 – 48Combined sources3
Beta strandi68 – 70Combined sources3
Helixi81 – 86Combined sources6
Beta strandi92 – 94Combined sources3
Helixi103 – 106Combined sources4
Helixi107 – 111Combined sources5
Beta strandi117 – 119Combined sources3
Helixi124 – 127Combined sources4
Helixi131 – 138Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JE0X-ray2.40A/B/C/D/E/F1-149[»]
2JE1X-ray2.69A/B/C/D1-149[»]
4XOSX-ray1.56A/B1-149[»]
ProteinModelPortaliP39687.
SMRiP39687.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39687.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati18 – 38LRR 11 PublicationAdd BLAST21
Repeati43 – 64LRR 21 PublicationAdd BLAST22
Repeati65 – 87LRR 31 PublicationAdd BLAST23
Repeati89 – 110LRR 41 PublicationAdd BLAST22
Domaini123 – 161LRRCTAdd BLAST39

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 174Necessary for tumor-suppressive functionAdd BLAST25
Regioni165 – 249Interaction with E4F1By similarityAdd BLAST85

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi168 – 249Asp/Glu-rich (highly acidic)Add BLAST82

Sequence similaritiesi

Belongs to the ANP32 family.Curated
Contains 4 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG2739. Eukaryota.
ENOG4111HZT. LUCA.
GeneTreeiENSGT00560000077130.
HOGENOMiHOG000007361.
HOVERGENiHBG053102.
InParanoidiP39687.
KOiK18646.
OMAiNCRSYEG.
OrthoDBiEOG091G0R8Z.
PhylomeDBiP39687.
TreeFamiTF317206.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003603. U2A'_phosphoprotein32A_C.
[Graphical view]
SMARTiSM00446. LRRcap. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39687-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEMGRRIHLE LRNRTPSDVK ELVLDNSRSN EGKLEGLTDE FEELEFLSTI
60 70 80 90 100
NVGLTSIANL PKLNKLKKLE LSDNRVSGGL EVLAEKCPNL THLNLSGNKI
110 120 130 140 150
KDLSTIEPLK KLENLKSLDL FNCEVTNLND YRENVFKLLP QLTYLDGYDR
160 170 180 190 200
DDKEAPDSDA EGYVEGLDDE EEDEDEEEYD EDAQVVEDEE DEDEEEEGEE
210 220 230 240
EDVSGEEEED EEGYNDGEVD DEEDEEELGE EERGQKRKRE PEDEGEDDD
Length:249
Mass (Da):28,585
Last modified:February 1, 1995 - v1
Checksum:iCA2D1A756FBAEA04
GO

Sequence cautioni

The sequence BAD97000 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti186V → A in BAD97000 (Ref. 8) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75090 mRNA. Translation: CAA52981.1.
U60823 mRNA. Translation: AAC50570.1.
U73477 mRNA. Translation: AAB39706.1.
AF025684 mRNA. Translation: AAB91548.1.
AY349171 mRNA. Translation: AAQ79832.1.
BT007436 mRNA. Translation: AAP36104.1.
AK223280 mRNA. Translation: BAD97000.1. Different initiation.
AK312703 mRNA. Translation: BAG35581.1.
CH471082 Genomic DNA. Translation: EAW77824.1.
BC007200 mRNA. Translation: AAH07200.1.
CCDSiCCDS45292.1.
PIRiS36375.
S43309.
RefSeqiNP_006296.1. NM_006305.3.
UniGeneiHs.458747.

Genome annotation databases

EnsembliENST00000465139; ENSP00000417864; ENSG00000140350.
GeneIDi8125.
KEGGihsa:8125.
UCSCiuc002arl.4. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75090 mRNA. Translation: CAA52981.1.
U60823 mRNA. Translation: AAC50570.1.
U73477 mRNA. Translation: AAB39706.1.
AF025684 mRNA. Translation: AAB91548.1.
AY349171 mRNA. Translation: AAQ79832.1.
BT007436 mRNA. Translation: AAP36104.1.
AK223280 mRNA. Translation: BAD97000.1. Different initiation.
AK312703 mRNA. Translation: BAG35581.1.
CH471082 Genomic DNA. Translation: EAW77824.1.
BC007200 mRNA. Translation: AAH07200.1.
CCDSiCCDS45292.1.
PIRiS36375.
S43309.
RefSeqiNP_006296.1. NM_006305.3.
UniGeneiHs.458747.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JE0X-ray2.40A/B/C/D/E/F1-149[»]
2JE1X-ray2.69A/B/C/D1-149[»]
4XOSX-ray1.56A/B1-149[»]
ProteinModelPortaliP39687.
SMRiP39687.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113791. 55 interactors.
IntActiP39687. 19 interactors.
MINTiMINT-4999627.
STRINGi9606.ENSP00000417864.

PTM databases

iPTMnetiP39687.
PhosphoSitePlusiP39687.
SwissPalmiP39687.

Polymorphism and mutation databases

BioMutaiANP32A.
DMDMi730318.

2D gel databases

SWISS-2DPAGEP39687.

Proteomic databases

EPDiP39687.
MaxQBiP39687.
PaxDbiP39687.
PeptideAtlasiP39687.
PRIDEiP39687.
TopDownProteomicsiP39687.

Protocols and materials databases

DNASUi8125.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000465139; ENSP00000417864; ENSG00000140350.
GeneIDi8125.
KEGGihsa:8125.
UCSCiuc002arl.4. human.

Organism-specific databases

CTDi8125.
DisGeNETi8125.
GeneCardsiANP32A.
HGNCiHGNC:13233. ANP32A.
HPAiCAB005231.
HPA063133.
HPA067561.
MIMi600832. gene.
neXtProtiNX_P39687.
OpenTargetsiENSG00000140350.
PharmGKBiPA24811.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2739. Eukaryota.
ENOG4111HZT. LUCA.
GeneTreeiENSGT00560000077130.
HOGENOMiHOG000007361.
HOVERGENiHBG053102.
InParanoidiP39687.
KOiK18646.
OMAiNCRSYEG.
OrthoDBiEOG091G0R8Z.
PhylomeDBiP39687.
TreeFamiTF317206.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000140350-MONOMER.
ReactomeiR-HSA-450520. HuR (ELAVL1) binds and stabilizes mRNA.

Miscellaneous databases

ChiTaRSiANP32A. human.
EvolutionaryTraceiP39687.
GenomeRNAii8125.
PMAP-CutDBP39687.
PROiP39687.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000140350.
CleanExiHS_ANP32A.
ExpressionAtlasiP39687. baseline and differential.
GenevisibleiP39687. HS.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003603. U2A'_phosphoprotein32A_C.
[Graphical view]
SMARTiSM00446. LRRcap. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAN32A_HUMAN
AccessioniPrimary (citable) accession number: P39687
Secondary accession number(s): B2R6T4
, Q53FK4, Q5J8L8, Q7M4N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 172 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.