P39685 (PO152_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoporin POM152

Alternative name(s):
Nuclear pore protein POM152
P150
Pore membrane protein POM152

Gene names

Name:	POM152
Ordered Locus Names:	YMR129W
ORF Names:	YM9553.05

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1337 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. POM152 is important for the de novo assembly of NPCs. Ref.4 Ref.5 Ref.7
Subunit structure	The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. Interacts with NUP188. Ref.4 Ref.6
Subcellular location	Nucleus › nuclear pore complex. Nucleus membrane; Multi-pass membrane protein. Note: Central core structure of the nuclear pore complex.
Post-translational modification	The N-terminus is blocked. Phosphorylated by CDC28. Ref.10 Ref.12 Ref.13 Ref.14 Ref.15
Miscellaneous	Present with 3410 molecules/cell in log phase SD medium. Ref.9

Ontologies

Keywords
Biological process	Protein transport Translocation Transport mRNA transport
Cellular component	Membrane Nuclear pore complex Nucleus
Domain	Repeat Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW protein import into nucleus Inferred from direct assay. Source: SGD
Cellular component	NDC1 complex Inferred from direct assay. Source: SGD mitochondrion Inferred from direct assay. Source: SGD nuclear envelope lumen Inferred from direct assay Ref.5. Source: SGD nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein anchor Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1337	1337	Nucleoporin POM152		PRO_0000204910
Regions
	Topological domain	1 – 110	110	Cytoplasmic Potential
	Transmembrane	111 – 131	21	Helical; Potential
	Topological domain	132 – 148	17	Perinuclear space Potential
	Transmembrane	149 – 169	21	Helical; Potential
	Topological domain	170 – 172	3	Cytoplasmic Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Topological domain	194 – 1337	1144	Perinuclear space Potential
	Repeat	390 – 413	24	1
	Repeat	626 – 650	25	2
	Repeat	732 – 755	24	3
	Repeat	836 – 859	24	4
	Repeat	943 – 966	24	5
	Repeat	1058 – 1077	20	6
	Repeat	1157 – 1178	22	7
	Repeat	1253 – 1276	24	8
	Region	1 – 175	175	Pore side Probable
	Region	196 – 1337	1142	Cisternal side Probable
	Region	390 – 1276	887	8 X 24 AA approximate repeats
Amino acid modifications
	Modified residue	45	1	Phosphoserine Ref.12 Ref.14 Ref.15
	Modified residue	60	1	Phosphoserine Ref.13
	Modified residue	787	1	Phosphothreonine Ref.15
	Modified residue	797	1	Phosphoserine Ref.15
	Glycosylation	280	1	N-linked (GlcNAc...) Probable

Sequences

	Sequence		Length	Mass (Da)
	P39685 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: A024A42069193898	FASTA	1,337	151,653
	10 20 30 40 50 60 MEHRYNVFND TPRGNHWMGS SVSGSPRPSY SSRPNVNTTR RFQYSDDEPA EKIRPLRSRS 70 80 90 100 110 120 FKSTESNISD EKSRISERDS KDRYINGDKK VDIYSLPLIS TDVLEISKQR TFAVILFLII 130 140 150 160 170 180 QCYKIYDLVI LKSGLPLSGL LFKNYRFNFI SKYFIIDSFF LYVLPSFNIP RLTFKPWVVY 190 200 210 220 230 240 LQILAMLLLN IFISSDHEFV LISLIMTTWR KLYTKELSVT GSAINHHRIF DSSAHFKGAL 250 260 270 280 290 300 TIKILPENTA MFNPLHESYC LPMDTNLFKI NSIDVPIRIN STEEIEYIEL EYRDLYTNSV 310 320 330 340 350 360 ELRSLSKKDF KIIDNPKSFL KKDQSVLKSH SNDFEEGSTI RYLAVTLQDI GFYQIKKIVD 370 380 390 400 410 420 SKKLNLKIHQ SHLVVPYCPI ASITGTGSND RCIGDSDNVS FEIQGVPPMK LAYSKIVNGQ 430 440 450 460 470 480 TFSYVDSSLQ PEYFESPLQS SKSKQSFTQG ELNDLKWGRN QPVNINLDSS ITQDGKFAYK 490 500 510 520 530 540 IDKITDGLGN VVDFTSLPEE LKKRYDLSYN FNVHEVPRAA LEERFDPKSP TKRSIAIVFE 550 560 570 580 590 600 EIKNWISDIP YVISLSYTDA QDKSKKIMNV TTDSLTKVLQ ADLPGSYNLE YIESKFCPGE 610 620 630 640 650 660 IVGKSNVLVT MPVAPTMEVK SFPILDQCVG QVGLNFELSF TGAPPYYYNT KIYKLENGER 670 680 690 700 710 720 KLYDAKRYTS EGTRNRFSYS PPKEGNYEIV FDTVSNKLFT EPIKLEPVKE YTFKTSMRVK 730 740 750 760 770 780 PSASLKLHHD LKLCLGDHSS VPVALKGQGP FTLTYDIIET FSSKRKTFEI KEIKTNEYVI 790 800 810 820 830 840 KTPVFTTGGD YILSLVSIKD STGCVVGLSQ PDAKIQVRRD IPSAAFNFFE PIKEAKIKHG 850 860 870 880 890 900 SVTEIPLKLS GEGPFTVKFK HMDYDGNIVK EFENKFQNSY KPALKVSKEG LYQLVDIRDS 910 920 930 940 950 960 SCQGNVIYRN SLYKVSFLEK PKFAIQDNHH ITKVTENLFS KEEVCQGMEG TVDLALFGSP 970 980 990 1000 1010 1020 PFILEYDLMA PNGHISTKKI QVATKYASLK LPNQIPGEYI TTIKAIFDGN YGESDIHFRE 1030 1040 1050 1060 1070 1080 HQSELIIKQT VHPIPDVAFA DGGKTLRACA ANVDQISFLE PINLKFLQGE SPFSITFSVY 1090 1100 1110 1120 1130 1140 HESTSRTDQY TIDNIDSENF SFEKLYEGMK LGNHAITIDS VVDANGCVNS LISGPRNQIL 1150 1160 1170 1180 1190 1200 VSITDAPKIH ILDPSTEYCV GDYVAYQLNG VAPFMIKYEF NGIPLKSKER SSQFVRLASE 1210 1220 1230 1240 1250 1260 PGIISITSLQ DSSSQCIVDF TNPKLKSEFD DLSLNIHPIP SVTVSQGNYV TEDIREGDQA 1270 1280 1290 1300 1310 1320 EVIFSFEGTP PFSLTYVRTE ETDGKHGKRR SQVVETHKVT DIYSHEYKVI TSLQGTYEAI 1330 EITDAYCFAK NDLFFNN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"POM152 is an integral protein of the pore membrane domain of the yeast nuclear envelope." Wozniak R.W., Blobel G., Rout M.P. J. Cell Biol. 125:31-42(1994) [PubMed: 8138573] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, GLYCOSYLATION, REPEATS. Strain: ATCC 200060 / W303.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex." Nehrbass U., Rout M.P., Maguire S., Blobel G., Wozniak R.W. J. Cell Biol. 133:1153-1162(1996) [PubMed: 8682855] [Abstract] Cited for: FUNCTION, INTERACTION WITH NUP188.
[5]	"Topology and functional domains of the yeast pore membrane protein Pom152p." Tcheperegine S.E., Marelli M., Wozniak R.W. J. Biol. Chem. 274:5252-5258(1999) [PubMed: 9988776] [Abstract] Cited for: FUNCTION, TOPOLOGY.
[6]	"The yeast nuclear pore complex: composition, architecture, and transport mechanism." Rout M.P., Aitchison J.D., Suprapto A., Hjertaas K., Zhao Y., Chait B.T. J. Cell Biol. 148:635-651(2000) [PubMed: 10684247] [Abstract] Cited for: CHARACTERIZATION, NPC SUBUNIT LOCATION.
[7]	"A link between the synthesis of nucleoporins and the biogenesis of the nuclear envelope." Marelli M., Lusk C.P., Chan H., Aitchison J.D., Wozniak R.W. J. Cell Biol. 153:709-724(2001) [PubMed: 11352933] [Abstract] Cited for: FUNCTION, DE NOVO NPC ASSEMBLY.
[8]	"Peering through the pore: nuclear pore complex structure, assembly, and function." Suntharalingam M., Wente S.R. Dev. Cell 4:775-789(2003) [PubMed: 12791264] [Abstract] Cited for: REVIEW.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Targets of the cyclin-dependent kinase Cdk1." Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K., Shokat K.M., Morgan D.O. Nature 425:859-864(2003) [PubMed: 14574415] [Abstract] Cited for: PHOSPHORYLATION BY CDC28.
[11]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[12]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45, MASS SPECTROMETRY. Strain: ADR376.
[13]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-60, MASS SPECTROMETRY.
[14]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45, MASS SPECTROMETRY.
[15]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; THR-787 AND SER-797, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z31592 Genomic DNA. Translation: CAA83469.1. Z48622 Genomic DNA. Translation: CAA88554.1. BK006946 Genomic DNA. Translation: DAA10026.1.
PIR	A53824.
RefSeq	NP_013848.1. NM_001182630.1.
3D structure databases
ProteinModelPortal	P39685.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1520N.
IntAct	P39685. 5 interactions.
MINT	MINT-389828.
STRING	P39685.
Protein family/group databases
TCDB	9.A.14.1.1. nuclear pore complex (NPC) family.
Proteomic databases
PeptideAtlas	P39685.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR129W; YMR129W; YMR129W.
GeneID	855159.
KEGG	sce:YMR129W.
NMPDR	fig|4932.3.peg.4897.
Organism-specific databases
CYGD	YMR129w.
SGD	S000004736. POM152.
Phylogenomic databases
eggNOG	fuNOG05029.
GeneTree	EFGT00050000003458.
HOGENOM	HBG203438.
OMA	TPPFEFT.
OrthoDB	EOG4GBBF8.
Gene expression databases
ArrayExpress	P39685.
Genevestigator	P39685.
GermOnline	YMR129W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	978578.

Entry information

Entry name

PO152_YEAST

Accession

Primary (citable) accession number: P39685
Secondary accession number(s): D6VZV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program