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P39683 (NPT1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nicotinate phosphoribosyltransferase

Short name=NAPRTase
EC=6.3.4.21
Gene names
Name:NPT1
Ordered Locus Names:YOR209C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential for growth under anaerobic conditions. Ref.5

Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP By similarity. Ref.5

Catalytic activity

Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate.

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.

Subcellular location

Cytoplasm. Nucleus Ref.6.

Miscellaneous

Present with 32100 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the NAPRTase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PRP40P332032EBI-12218,EBI-701
RSP5P399402EBI-12218,EBI-16219

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429Nicotinate phosphoribosyltransferase
PRO_0000205862

Experimental info

Sequence conflict131 – 1377YEIPLLS → MRSLTV in CAA85352. Ref.1
Sequence conflict144 – 15815FKFVD…ENQLE → LIVTSTGLRNHR Ref.1
Sequence conflict175 – 1773SEF → RIH in CAA85352. Ref.1
Sequence conflict2431E → EL in CAA85352. Ref.1

Secondary structure

................................................................ 429
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P39683 [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: F3F7941476C3398B

FASTA42949,019
        10         20         30         40         50         60 
MSEPVIKSLL DTDMYKITMH AAVFTNFPDV TVTYKYTNRS SQLTFNKEAI NWLKEQFSYL 

        70         80         90        100        110        120 
GNLRFTEEEI EYLKQEIPYL PSAYIKYISS SNYKLHPEEQ ISFTSEEIEG KPTHYKLKIL 

       130        140        150        160        170        180 
VSGSWKDTIL YEIPLLSLIS EAYFKFVDID WDYENQLEQA EKKAETLFDN GIRFSEFGTR 

       190        200        210        220        230        240 
RRRSLKAQDL IMQGIMKAVN GNPDRNKSLL LGTSNILFAK KYGVKPIGTV AHEWVMGVAS 

       250        260        270        280        290        300 
ISEDYLHANK NAMDCWINTF GAKNAGLALT DTFGTDDFLK SFRPPYSDAY VGVRQDSGDP 

       310        320        330        340        350        360 
VEYTKKISHH YHDVLKLPKF SKIICYSDSL NVEKAITYSH AAKENGMLAT FGIGTNFTND 

       370        380        390        400        410        420 
FRKKSEPQVK SEPLNIVIKL LEVNGNHAIK ISDNLGKNMG DPATVKRVKE ELGYTERSWS 


GDNEAHRWT 

« Hide

References

« Hide 'large scale' references
[1]"The BRF1-STE4 region chromosome XV contains eleven genes: two of them define distinct RNA polymerase subunits and one encodes a putative nicotinate phosphorybosyl transferase."
Lalo D., Doira C., Thuriaux P.
Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Interactions between three common subunits of yeast RNA polymerases I and III."
Lalo D., Carles C., Sentenac A., Thuriaux P.
Proc. Natl. Acad. Sci. U.S.A. 90:5524-5528(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-43.
Strain: ATCC 28383 / FL100 / VTT C-80102.
[5]"Aerobic and anaerobic NAD+ metabolism in Saccharomyces cerevisiae."
Panozzo C., Nawara M., Suski C., Kucharczyka R., Skoneczny M., Becam A.-M., Rytka J., Herbert C.J.
FEBS Lett. 517:97-102(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z36878 Genomic DNA. Translation: CAA85352.1.
Z75117 Genomic DNA. Translation: CAA99424.1.
L11274 Genomic DNA. Translation: AAB59317.1.
BK006948 Genomic DNA. Translation: DAA10981.1.
PIRS67101.
RefSeqNP_014852.1. NM_001183628.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1VLPX-ray1.75A/B/C/D1-429[»]
ProteinModelPortalP39683.
SMRP39683. Positions 1-419.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34604. 163 interactions.
DIPDIP-4984N.
IntActP39683. 8 interactions.
MINTMINT-567492.
STRING4932.YOR209C.

Proteomic databases

MaxQBP39683.
PaxDbP39683.
PeptideAtlasP39683.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYOR209C; YOR209C; YOR209C.
GeneID854384.
KEGGsce:YOR209C.

Organism-specific databases

CYGDYOR209c.
SGDS000005735. NPT1.

Phylogenomic databases

eggNOGCOG1488.
HOGENOMHOG000284928.
KOK00763.
OMADWTYEGQ.
OrthoDBEOG71K6C8.

Enzyme and pathway databases

BioCycYEAST:YOR209C-MONOMER.
BRENDA2.4.2.11. 984.
UniPathwayUPA00253; UER00457.

Gene expression databases

GenevestigatorP39683.

Family and domain databases

InterProIPR006406. Nic_PRibTrfase.
IPR007229. Nic_PRibTrfase-Fam.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view]
PfamPF04095. NAPRTase. 1 hit.
[Graphical view]
PIRSFPIRSF000484. NAPRT. 1 hit.
SUPFAMSSF51690. SSF51690. 1 hit.
TIGRFAMsTIGR01514. NAPRTase. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP39683.
NextBio976529.
PROP39683.

Entry information

Entry nameNPT1_YEAST
AccessionPrimary (citable) accession number: P39683
Secondary accession number(s): D6W2R5, Q08626
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XV

Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways