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P39683

- NPT1_YEAST

UniProt

P39683 - NPT1_YEAST

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Protein
Nicotinate phosphoribosyltransferase
Gene
NPT1, YOR209C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential for growth under anaerobic conditions.1 Publication
Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP By similarity.1 Publication

Catalytic activityi

Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate.

Pathwayi

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. nicotinate phosphoribosyltransferase activity Source: SGD
  3. nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: InterPro
  4. protein binding Source: IntAct

GO - Biological processi

  1. NAD biosynthetic process Source: UniProtKB-UniPathway
  2. chromatin silencing at rDNA Source: SGD
  3. chromatin silencing at telomere Source: SGD
  4. nicotinate nucleotide salvage Source: SGD
  5. replicative cell aging Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YOR209C-MONOMER.
BRENDAi2.4.2.11. 984.
UniPathwayiUPA00253; UER00457.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate phosphoribosyltransferase (EC:6.3.4.21)
Short name:
NAPRTase
Gene namesi
Name:NPT1
Ordered Locus Names:YOR209C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

CYGDiYOR209c.
SGDiS000005735. NPT1.

Subcellular locationi

Cytoplasm. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 429429Nicotinate phosphoribosyltransferase
PRO_0000205862Add
BLAST

Proteomic databases

MaxQBiP39683.
PaxDbiP39683.
PeptideAtlasiP39683.

Expressioni

Gene expression databases

GenevestigatoriP39683.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP40P332032EBI-12218,EBI-701
RSP5P399402EBI-12218,EBI-16219

Protein-protein interaction databases

BioGridi34604. 163 interactions.
DIPiDIP-4984N.
IntActiP39683. 8 interactions.
MINTiMINT-567492.
STRINGi4932.YOR209C.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 133
Helixi14 – 2613
Beta strandi31 – 399
Helixi47 – 5913
Helixi60 – 623
Helixi67 – 7610
Helixi82 – 898
Helixi97 – 1004
Beta strandi101 – 1088
Beta strandi111 – 12414
Helixi125 – 1284
Helixi132 – 14615
Helixi156 – 16914
Beta strandi174 – 1763
Helixi185 – 20117
Helixi203 – 2064
Turni207 – 2093
Beta strandi210 – 2156
Helixi216 – 2227
Helixi232 – 24211
Helixi248 – 26013
Helixi262 – 2643
Beta strandi267 – 2693
Helixi275 – 2795
Helixi286 – 2894
Beta strandi292 – 2954
Helixi300 – 31213
Beta strandi321 – 3266
Helixi332 – 34413
Beta strandi348 – 3536
Helixi355 – 3584
Beta strandi364 – 3663
Beta strandi369 – 3713
Beta strandi376 – 3838
Helixi402 – 41110

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VLPX-ray1.75A/B/C/D1-429[»]
ProteinModelPortaliP39683.
SMRiP39683. Positions 1-419.

Miscellaneous databases

EvolutionaryTraceiP39683.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAPRTase family.

Phylogenomic databases

eggNOGiCOG1488.
HOGENOMiHOG000284928.
KOiK00763.
OMAiDWTYEGQ.
OrthoDBiEOG71K6C8.

Family and domain databases

InterProiIPR006406. Nic_PRibTrfase.
IPR007229. Nic_PRibTrfase-Fam.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view]
PANTHERiPTHR11098. PTHR11098. 1 hit.
PfamiPF04095. NAPRTase. 1 hit.
[Graphical view]
PIRSFiPIRSF000484. NAPRT. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR01514. NAPRTase. 1 hit.

Sequencei

Sequence statusi: Complete.

P39683-1 [UniParc]FASTAAdd to Basket

« Hide

MSEPVIKSLL DTDMYKITMH AAVFTNFPDV TVTYKYTNRS SQLTFNKEAI    50
NWLKEQFSYL GNLRFTEEEI EYLKQEIPYL PSAYIKYISS SNYKLHPEEQ 100
ISFTSEEIEG KPTHYKLKIL VSGSWKDTIL YEIPLLSLIS EAYFKFVDID 150
WDYENQLEQA EKKAETLFDN GIRFSEFGTR RRRSLKAQDL IMQGIMKAVN 200
GNPDRNKSLL LGTSNILFAK KYGVKPIGTV AHEWVMGVAS ISEDYLHANK 250
NAMDCWINTF GAKNAGLALT DTFGTDDFLK SFRPPYSDAY VGVRQDSGDP 300
VEYTKKISHH YHDVLKLPKF SKIICYSDSL NVEKAITYSH AAKENGMLAT 350
FGIGTNFTND FRKKSEPQVK SEPLNIVIKL LEVNGNHAIK ISDNLGKNMG 400
DPATVKRVKE ELGYTERSWS GDNEAHRWT 429
Length:429
Mass (Da):49,019
Last modified:November 1, 1997 - v3
Checksum:iF3F7941476C3398B
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti131 – 1377YEIPLLS → MRSLTV in CAA85352. 1 Publication
Sequence conflicti144 – 15815FKFVD…ENQLE → LIVTSTGLRNHR1 Publication
Add
BLAST
Sequence conflicti175 – 1773SEF → RIH in CAA85352. 1 Publication
Sequence conflicti243 – 2431E → EL in CAA85352. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z36878 Genomic DNA. Translation: CAA85352.1.
Z75117 Genomic DNA. Translation: CAA99424.1.
L11274 Genomic DNA. Translation: AAB59317.1.
BK006948 Genomic DNA. Translation: DAA10981.1.
PIRiS67101.
RefSeqiNP_014852.1. NM_001183628.1.

Genome annotation databases

EnsemblFungiiYOR209C; YOR209C; YOR209C.
GeneIDi854384.
KEGGisce:YOR209C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z36878 Genomic DNA. Translation: CAA85352.1 .
Z75117 Genomic DNA. Translation: CAA99424.1 .
L11274 Genomic DNA. Translation: AAB59317.1 .
BK006948 Genomic DNA. Translation: DAA10981.1 .
PIRi S67101.
RefSeqi NP_014852.1. NM_001183628.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VLP X-ray 1.75 A/B/C/D 1-429 [» ]
ProteinModelPortali P39683.
SMRi P39683. Positions 1-419.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34604. 163 interactions.
DIPi DIP-4984N.
IntActi P39683. 8 interactions.
MINTi MINT-567492.
STRINGi 4932.YOR209C.

Proteomic databases

MaxQBi P39683.
PaxDbi P39683.
PeptideAtlasi P39683.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR209C ; YOR209C ; YOR209C .
GeneIDi 854384.
KEGGi sce:YOR209C.

Organism-specific databases

CYGDi YOR209c.
SGDi S000005735. NPT1.

Phylogenomic databases

eggNOGi COG1488.
HOGENOMi HOG000284928.
KOi K00763.
OMAi DWTYEGQ.
OrthoDBi EOG71K6C8.

Enzyme and pathway databases

UniPathwayi UPA00253 ; UER00457 .
BioCyci YEAST:YOR209C-MONOMER.
BRENDAi 2.4.2.11. 984.

Miscellaneous databases

EvolutionaryTracei P39683.
NextBioi 976529.
PROi P39683.

Gene expression databases

Genevestigatori P39683.

Family and domain databases

InterProi IPR006406. Nic_PRibTrfase.
IPR007229. Nic_PRibTrfase-Fam.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view ]
PANTHERi PTHR11098. PTHR11098. 1 hit.
Pfami PF04095. NAPRTase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000484. NAPRT. 1 hit.
SUPFAMi SSF51690. SSF51690. 1 hit.
TIGRFAMsi TIGR01514. NAPRTase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The BRF1-STE4 region chromosome XV contains eleven genes: two of them define distinct RNA polymerase subunits and one encodes a putative nicotinate phosphorybosyl transferase."
    Lalo D., Doira C., Thuriaux P.
    Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Interactions between three common subunits of yeast RNA polymerases I and III."
    Lalo D., Carles C., Sentenac A., Thuriaux P.
    Proc. Natl. Acad. Sci. U.S.A. 90:5524-5528(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-43.
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  5. "Aerobic and anaerobic NAD+ metabolism in Saccharomyces cerevisiae."
    Panozzo C., Nawara M., Suski C., Kucharczyka R., Skoneczny M., Becam A.-M., Rytka J., Herbert C.J.
    FEBS Lett. 517:97-102(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNPT1_YEAST
AccessioniPrimary (citable) accession number: P39683
Secondary accession number(s): D6W2R5, Q08626
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 32100 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

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