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P39683

- NPT1_YEAST

UniProt

P39683 - NPT1_YEAST

Protein

Nicotinate phosphoribosyltransferase

Gene

NPT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 3 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Essential for growth under anaerobic conditions.1 Publication
    Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.By similarity

    Catalytic activityi

    Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate.

    Pathwayi

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: InterPro
    3. nicotinate phosphoribosyltransferase activity Source: SGD
    4. protein binding Source: IntAct

    GO - Biological processi

    1. chromatin silencing at rDNA Source: SGD
    2. chromatin silencing at telomere Source: SGD
    3. NAD biosynthetic process Source: UniProtKB-UniPathway
    4. nicotinate nucleotide salvage Source: SGD
    5. replicative cell aging Source: SGD

    Keywords - Molecular functioni

    Ligase, Transferase

    Keywords - Biological processi

    Pyridine nucleotide biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:YOR209C-MONOMER.
    BRENDAi2.4.2.11. 984.
    UniPathwayiUPA00253; UER00457.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nicotinate phosphoribosyltransferase (EC:6.3.4.21)
    Short name:
    NAPRTase
    Gene namesi
    Name:NPT1
    Ordered Locus Names:YOR209C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XV

    Organism-specific databases

    CYGDiYOR209c.
    SGDiS000005735. NPT1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 429429Nicotinate phosphoribosyltransferasePRO_0000205862Add
    BLAST

    Proteomic databases

    MaxQBiP39683.
    PaxDbiP39683.
    PeptideAtlasiP39683.

    Expressioni

    Gene expression databases

    GenevestigatoriP39683.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PRP40P332032EBI-12218,EBI-701
    RSP5P399402EBI-12218,EBI-16219

    Protein-protein interaction databases

    BioGridi34604. 163 interactions.
    DIPiDIP-4984N.
    IntActiP39683. 8 interactions.
    MINTiMINT-567492.
    STRINGi4932.YOR209C.

    Structurei

    Secondary structure

    1
    429
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi11 – 133
    Helixi14 – 2613
    Beta strandi31 – 399
    Helixi47 – 5913
    Helixi60 – 623
    Helixi67 – 7610
    Helixi82 – 898
    Helixi97 – 1004
    Beta strandi101 – 1088
    Beta strandi111 – 12414
    Helixi125 – 1284
    Helixi132 – 14615
    Helixi156 – 16914
    Beta strandi174 – 1763
    Helixi185 – 20117
    Helixi203 – 2064
    Turni207 – 2093
    Beta strandi210 – 2156
    Helixi216 – 2227
    Helixi232 – 24211
    Helixi248 – 26013
    Helixi262 – 2643
    Beta strandi267 – 2693
    Helixi275 – 2795
    Helixi286 – 2894
    Beta strandi292 – 2954
    Helixi300 – 31213
    Beta strandi321 – 3266
    Helixi332 – 34413
    Beta strandi348 – 3536
    Helixi355 – 3584
    Beta strandi364 – 3663
    Beta strandi369 – 3713
    Beta strandi376 – 3838
    Helixi402 – 41110

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1VLPX-ray1.75A/B/C/D1-429[»]
    ProteinModelPortaliP39683.
    SMRiP39683. Positions 1-419.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP39683.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAPRTase family.Curated

    Phylogenomic databases

    eggNOGiCOG1488.
    HOGENOMiHOG000284928.
    KOiK00763.
    OMAiDWTYEGQ.
    OrthoDBiEOG71K6C8.

    Family and domain databases

    InterProiIPR006406. Nic_PRibTrfase.
    IPR007229. Nic_PRibTrfase-Fam.
    IPR002638. Quinolinate_PRibosylTrfase_C.
    [Graphical view]
    PANTHERiPTHR11098. PTHR11098. 1 hit.
    PfamiPF04095. NAPRTase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000484. NAPRT. 1 hit.
    SUPFAMiSSF51690. SSF51690. 1 hit.
    TIGRFAMsiTIGR01514. NAPRTase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P39683-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEPVIKSLL DTDMYKITMH AAVFTNFPDV TVTYKYTNRS SQLTFNKEAI    50
    NWLKEQFSYL GNLRFTEEEI EYLKQEIPYL PSAYIKYISS SNYKLHPEEQ 100
    ISFTSEEIEG KPTHYKLKIL VSGSWKDTIL YEIPLLSLIS EAYFKFVDID 150
    WDYENQLEQA EKKAETLFDN GIRFSEFGTR RRRSLKAQDL IMQGIMKAVN 200
    GNPDRNKSLL LGTSNILFAK KYGVKPIGTV AHEWVMGVAS ISEDYLHANK 250
    NAMDCWINTF GAKNAGLALT DTFGTDDFLK SFRPPYSDAY VGVRQDSGDP 300
    VEYTKKISHH YHDVLKLPKF SKIICYSDSL NVEKAITYSH AAKENGMLAT 350
    FGIGTNFTND FRKKSEPQVK SEPLNIVIKL LEVNGNHAIK ISDNLGKNMG 400
    DPATVKRVKE ELGYTERSWS GDNEAHRWT 429
    Length:429
    Mass (Da):49,019
    Last modified:November 1, 1997 - v3
    Checksum:iF3F7941476C3398B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti131 – 1377YEIPLLS → MRSLTV in CAA85352. 1 PublicationCurated
    Sequence conflicti144 – 15815FKFVD…ENQLE → LIVTSTGLRNHR1 PublicationCuratedAdd
    BLAST
    Sequence conflicti175 – 1773SEF → RIH in CAA85352. 1 PublicationCurated
    Sequence conflicti243 – 2431E → EL in CAA85352. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36878 Genomic DNA. Translation: CAA85352.1.
    Z75117 Genomic DNA. Translation: CAA99424.1.
    L11274 Genomic DNA. Translation: AAB59317.1.
    BK006948 Genomic DNA. Translation: DAA10981.1.
    PIRiS67101.
    RefSeqiNP_014852.1. NM_001183628.1.

    Genome annotation databases

    EnsemblFungiiYOR209C; YOR209C; YOR209C.
    GeneIDi854384.
    KEGGisce:YOR209C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z36878 Genomic DNA. Translation: CAA85352.1 .
    Z75117 Genomic DNA. Translation: CAA99424.1 .
    L11274 Genomic DNA. Translation: AAB59317.1 .
    BK006948 Genomic DNA. Translation: DAA10981.1 .
    PIRi S67101.
    RefSeqi NP_014852.1. NM_001183628.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1VLP X-ray 1.75 A/B/C/D 1-429 [» ]
    ProteinModelPortali P39683.
    SMRi P39683. Positions 1-419.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34604. 163 interactions.
    DIPi DIP-4984N.
    IntActi P39683. 8 interactions.
    MINTi MINT-567492.
    STRINGi 4932.YOR209C.

    Proteomic databases

    MaxQBi P39683.
    PaxDbi P39683.
    PeptideAtlasi P39683.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YOR209C ; YOR209C ; YOR209C .
    GeneIDi 854384.
    KEGGi sce:YOR209C.

    Organism-specific databases

    CYGDi YOR209c.
    SGDi S000005735. NPT1.

    Phylogenomic databases

    eggNOGi COG1488.
    HOGENOMi HOG000284928.
    KOi K00763.
    OMAi DWTYEGQ.
    OrthoDBi EOG71K6C8.

    Enzyme and pathway databases

    UniPathwayi UPA00253 ; UER00457 .
    BioCyci YEAST:YOR209C-MONOMER.
    BRENDAi 2.4.2.11. 984.

    Miscellaneous databases

    EvolutionaryTracei P39683.
    NextBioi 976529.
    PROi P39683.

    Gene expression databases

    Genevestigatori P39683.

    Family and domain databases

    InterProi IPR006406. Nic_PRibTrfase.
    IPR007229. Nic_PRibTrfase-Fam.
    IPR002638. Quinolinate_PRibosylTrfase_C.
    [Graphical view ]
    PANTHERi PTHR11098. PTHR11098. 1 hit.
    Pfami PF04095. NAPRTase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000484. NAPRT. 1 hit.
    SUPFAMi SSF51690. SSF51690. 1 hit.
    TIGRFAMsi TIGR01514. NAPRTase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The BRF1-STE4 region chromosome XV contains eleven genes: two of them define distinct RNA polymerase subunits and one encodes a putative nicotinate phosphorybosyl transferase."
      Lalo D., Doira C., Thuriaux P.
      Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
      Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
      , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
      Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Interactions between three common subunits of yeast RNA polymerases I and III."
      Lalo D., Carles C., Sentenac A., Thuriaux P.
      Proc. Natl. Acad. Sci. U.S.A. 90:5524-5528(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-43.
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    5. "Aerobic and anaerobic NAD+ metabolism in Saccharomyces cerevisiae."
      Panozzo C., Nawara M., Suski C., Kucharczyka R., Skoneczny M., Becam A.-M., Rytka J., Herbert C.J.
      FEBS Lett. 517:97-102(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNPT1_YEAST
    AccessioniPrimary (citable) accession number: P39683
    Secondary accession number(s): D6W2R5, Q08626
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 130 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 32100 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome XV
      Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

    External Data

    Dasty 3