P39678 (MBP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription factor MBP1

Alternative name(s):
MBF subunit p120

Gene names

Name:	MBP1
Ordered Locus Names:	YDL056W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	833 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to MCB elements (Mlu I cell cycle box) found in the promoter of most DNA synthesis genes. Transcriptional activation by MBF has an important role in the transition from G1 to S phase. It may have a dual role in that it behaves as an activator of transcription at the G1-S boundary and as a repressor during other stages of the cell cycle.
Subunit structure	Component of the transcription complex MCB-binding factor (MBF) composed of SWI6 and MBP1. Interacts with MSA1. Ref.7
Subcellular location	Nucleus.
Miscellaneous	Present with 521 molecules/cell in log phase SD medium.
Sequence similarities	Contains 2 ANK repeats. Contains 1 HTH APSES-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	ANK repeat Repeat
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription involved in G1/S phase of mitotic cell cycle Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	MBF transcription complex Inferred from direct assay Ref.1. Source: SGD
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Inferred from physical interaction Ref.1. Source: SGD sequence-specific DNA binding Inferred from direct assay PubMed 19111667PubMed 19158363. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SWI6	P09959	5	EBI-10485,EBI-18641

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 833

833

Transcription factor MBP1

PRO_0000067062

Regions

Domain

5 – 111

107

HTH APSES-type

Repeat

394 – 423

30

ANK 1

Repeat

512 – 541

30

ANK 2

DNA binding

36 – 57

22

H-T-H motif

Amino acid modifications

Modified residue

110

1

Phosphoserine Ref.8

Modified residue

133

1

Phosphoserine Ref.8

Modified residue

135

1

Phosphoserine Ref.8

Modified residue

191

1

Phosphoserine Ref.6 Ref.8

Modified residue

212

1

Phosphoserine Ref.8

Modified residue

221

1

Phosphoserine Ref.8

Modified residue

326

1

Phosphoserine Ref.8

Modified residue

602

1

Phosphoserine Ref.8

Modified residue

827

1

Phosphoserine Ref.8

Secondary structure

1

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833

Helix Strand Turn

Details...

Beta strand

5 – 10

6

Beta strand

13 – 19

7

Beta strand

24 – 28

5

Turn

29 – 31

3

Beta strand

33 – 35

3

Helix

36 – 42

7

Helix

47 – 57

11

Turn

58 – 60

3

Beta strand

64 – 69

6

Turn

71 – 73

3

Beta strand

75 – 78

4

Helix

80 – 89

10

Helix

93 – 101

9

Sequences

	Sequence		Length	Mass (Da)
	P39678 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: BB7C35E29802BBD5	FASTA	833	93,908
	10 20 30 40 50 60 MSNQIYSARY SGVDVYEFIH STGSIMKRKK DDWVNATHIL KAANFAKAKR TRILEKEVLK 70 80 90 100 110 120 ETHEKVQGGF GKYQGTWVPL NIAKQLAEKF SVYDQLKPLF DFTQTDGSAS PPPAPKHHHA 130 140 150 160 170 180 SKVDRKKAIR SASTSAIMET KRNNKKAEEN QFQSSKILGN PTAAPRKRGR PVGSTRGSRR 190 200 210 220 230 240 KLGVNLQRSQ SDMGFPRPAI PNSSISTTQL PSIRSTMGPQ SPTLGILEEE RHDSRQQQPQ 250 260 270 280 290 300 QNNSAQFKEI DLEDGLSSDV EPSQQLQQVF NQNTGFVPQQ QSSLIQTQQT ESMATSVSSS 310 320 330 340 350 360 PSLPTSPGDF ADSNPFEERF PGGGTSPIIS MIPRYPVTSR PQTSDINDKV NKYLSKLVDY 370 380 390 400 410 420 FISNEMKSNK SLPQVLLHPP PHSAPYIDAP IDPELHTAFH WACSMGNLPI AEALYEAGTS 430 440 450 460 470 480 IRSTNSQGQT PLMRSSLFHN SYTRRTFPRI FQLLHETVFD IDSQSQTVIH HIVKRKSTTP 490 500 510 520 530 540 SAVYYLDVVL SKIKDFSPQY RIELLLNTQD KNGDTALHIA SKNGDVVFFN TLVKMGALTT 550 560 570 580 590 600 ISNKEGLTAN EIMNQQYEQM MIQNGTNQHV NSSNTDLNIH VNTNNIETKN DVNSMVIMSP 610 620 630 640 650 660 VSPSDYITYP SQIATNISRN IPNVVNSMKQ MASIYNDLHE QHDNEIKSLQ KTLKSISKTK 670 680 690 700 710 720 IQVSLKTLEV LKESSKDENG EAQTNDDFEI LSRLQEQNTK KLRKRLIRYK RLIKQKLEYR 730 740 750 760 770 780 QTVLLNKLIE DETQATTNNT VEKDNNTLER LELAQELTML QLQRKNKLSS LVKKFEDNAK 790 800 810 820 830 IHKYRRIIRE GTEMNIEEVD SSLDVILQTL IANNNKNKGA EQIITISNAN SHA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase." Koch C., Moll T., Neuberg M., Ahorn H., Nasmyth K. Science 261:1551-1557(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 372-387. Strain: K1107.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV." Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P. Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Over-expression of S. cerevisiae G1 cyclins restores the viability of alg1 N-glycosylation mutants." Benton B.K., Plump S.D., Roos J., Lennarz W.J., Cross F.R. Curr. Genet. 29:106-113(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 460-833.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-191, MASS SPECTROMETRY.
[7]	"The SBF- and MBF-associated protein Msa1 is required for proper timing of G1-specific transcription in Saccharomyces cerevisiae." Ashe M., de Bruin R.A.M., Kalashnikova T., McDonald W.H., Yates J.R. III, Wittenberg C. J. Biol. Chem. 283:6040-6049(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MSA1.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110; SER-133; SER-135; SER-191; SER-212; SER-221; SER-326; SER-602 AND SER-827, MASS SPECTROMETRY.
[9]	"The X-ray structure of the DNA-binding domain from the Saccharomyces cerevisiae cell-cycle transcription factor Mbp1 at 2.1-A resolution." Taylor I.A., Treiber M.K., Olivi L., Smerdon S.J. J. Mol. Biol. 272:1-8(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 1-100.
[10]	"Crystal structure of the DNA-binding domain of Mbp1, a transcription factor important in cell-cycle control of DNA synthesis." Xu R.M., Koch C., Liu Y., Horton J.R., Knapp D., Nasmyth K., Cheng X. Structure 5:349-358(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.71 ANGSTROMS) OF 1-102.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X74158 Genomic DNA. Translation: CAA52271.1.
Z74104 Genomic DNA. Translation: CAA98618.1.
U19608 Genomic DNA. Translation: AAC49290.1.
BK006938 Genomic DNA. Translation: DAA11800.1.

PIR

A47528.

RefSeq

NP_010227.1. NM_001180115.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BM8	X-ray	1.71	A	4-102	[»]
1L3G	NMR	-	A	2-124	[»]
1MB1	X-ray	2.10	A	1-124	[»]

ProteinModelPortal

P39678.

SMR

P39678. Positions 2-124, 349-547.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2391N.

IntAct

P39678. 29 interactions.

MINT

MINT-616665.

Proteomic databases

PaxDb

P39678.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YDL056W; YDL056W; YDL056W.

GeneID

851503.

KEGG

sce:YDL056W.

Organism-specific databases

CYGD

YDL056w.

SGD

S000002214. MBP1.

Phylogenomic databases

eggNOG

COG0666.

GeneTree

ENSGT00530000067565.

HOGENOM

HOG000113493.

KO

K06647.

OMA

HTAFHWA.

OrthoDB

EOG4PRWZZ.

Gene expression databases

ArrayExpress

P39678.

Genevestigator

P39678.

GermOnline

YDL056W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.25.40.20. 1 hit.
3.10.260.10. 1 hit.

InterPro

IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018004. KilA_N/APSES_HTH.
IPR003163. Tscrpt_reg_HTH_APSES-type.
[Graphical view]

Pfam

PF00023. Ank. 2 hits.
PF04383. KilA-N. 1 hit.
[Graphical view]

SMART

SM00248. ANK. 3 hits.
[Graphical view]

SUPFAM

SSF48403. ANK. 1 hit.
SSF54616. Mlu1-box-bd_prot_DNA-bd. 1 hit.

PROSITE

PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS51299. HTH_APSES. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P39678.

NextBio

968852.

Entry information

Entry name

MBP1_YEAST

Accession

Primary (citable) accession number: P39678
Secondary accession number(s): D6VRU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 139 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program