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Protein

Transcription factor MBP1

Gene

MBP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to MCB elements (Mlu I cell cycle box) found in the promoter of most DNA synthesis genes. Transcriptional activation by MBF has an important role in the transition from G1 to S phase. It may have a dual role in that it behaves as an activator of transcription at the G1-S boundary and as a repressor during other stages of the cell cycle.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi36 – 57H-T-H motifPROSITE-ProRule annotationAdd BLAST22

GO - Molecular functioni

GO - Biological processi

  • positive regulation of transcription involved in G1/S transition of mitotic cell cycle Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29472-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor MBP1
Alternative name(s):
MBF subunit p120
Gene namesi
Name:MBP1
Ordered Locus Names:YDL056W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL056W.
SGDiS000002214. MBP1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • MBF transcription complex Source: SGD
  • nuclear chromatin Source: GO_Central
  • SBF transcription complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670621 – 833Transcription factor MBP1Add BLAST833

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei110PhosphoserineCombined sources1
Modified residuei325PhosphothreonineCombined sources1
Modified residuei326PhosphoserineCombined sources1
Modified residuei330PhosphoserineCombined sources1
Modified residuei827PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39678.
PRIDEiP39678.

PTM databases

iPTMnetiP39678.

Interactioni

Subunit structurei

Component of the transcription complex MCB-binding factor (MBF) composed of SWI6 and MBP1. Interacts with MSA1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
SWI6P099595EBI-10485,EBI-18641

Protein-protein interaction databases

BioGridi32002. 95 interactors.
DIPiDIP-2391N.
IntActiP39678. 28 interactors.
MINTiMINT-616665.

Structurei

Secondary structure

1833
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Beta strandi13 – 19Combined sources7
Beta strandi24 – 28Combined sources5
Turni29 – 31Combined sources3
Beta strandi33 – 35Combined sources3
Helixi36 – 42Combined sources7
Helixi47 – 57Combined sources11
Turni58 – 60Combined sources3
Beta strandi64 – 69Combined sources6
Turni71 – 73Combined sources3
Beta strandi75 – 78Combined sources4
Helixi80 – 89Combined sources10
Helixi93 – 101Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BM8X-ray1.71A4-102[»]
1L3GNMR-A2-124[»]
1MB1X-ray2.10A1-124[»]
ProteinModelPortaliP39678.
SMRiP39678.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP39678.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 111HTH APSES-typePROSITE-ProRule annotationAdd BLAST107
Repeati394 – 423ANK 1Add BLAST30
Repeati512 – 541ANK 2Add BLAST30

Sequence similaritiesi

Contains 2 ANK repeats.PROSITE-ProRule annotation
Contains 1 HTH APSES-type DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

GeneTreeiENSGT00530000067565.
HOGENOMiHOG000113493.
InParanoidiP39678.
KOiK06647.
OMAiGFGKYQG.
OrthoDBiEOG092C16YL.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.10.260.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018004. KilA_N/APSES_HTH.
IPR029793. Mbp1/Res1/Res2.
IPR003163. Tscrpt_reg_HTH_APSES-type.
[Graphical view]
PANTHERiPTHR11707:SF30. PTHR11707:SF30. 2 hits.
PfamiPF04383. KilA-N. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM01252. KilA-N. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF54616. SSF54616. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS51299. HTH_APSES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39678-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNQIYSARY SGVDVYEFIH STGSIMKRKK DDWVNATHIL KAANFAKAKR
60 70 80 90 100
TRILEKEVLK ETHEKVQGGF GKYQGTWVPL NIAKQLAEKF SVYDQLKPLF
110 120 130 140 150
DFTQTDGSAS PPPAPKHHHA SKVDRKKAIR SASTSAIMET KRNNKKAEEN
160 170 180 190 200
QFQSSKILGN PTAAPRKRGR PVGSTRGSRR KLGVNLQRSQ SDMGFPRPAI
210 220 230 240 250
PNSSISTTQL PSIRSTMGPQ SPTLGILEEE RHDSRQQQPQ QNNSAQFKEI
260 270 280 290 300
DLEDGLSSDV EPSQQLQQVF NQNTGFVPQQ QSSLIQTQQT ESMATSVSSS
310 320 330 340 350
PSLPTSPGDF ADSNPFEERF PGGGTSPIIS MIPRYPVTSR PQTSDINDKV
360 370 380 390 400
NKYLSKLVDY FISNEMKSNK SLPQVLLHPP PHSAPYIDAP IDPELHTAFH
410 420 430 440 450
WACSMGNLPI AEALYEAGTS IRSTNSQGQT PLMRSSLFHN SYTRRTFPRI
460 470 480 490 500
FQLLHETVFD IDSQSQTVIH HIVKRKSTTP SAVYYLDVVL SKIKDFSPQY
510 520 530 540 550
RIELLLNTQD KNGDTALHIA SKNGDVVFFN TLVKMGALTT ISNKEGLTAN
560 570 580 590 600
EIMNQQYEQM MIQNGTNQHV NSSNTDLNIH VNTNNIETKN DVNSMVIMSP
610 620 630 640 650
VSPSDYITYP SQIATNISRN IPNVVNSMKQ MASIYNDLHE QHDNEIKSLQ
660 670 680 690 700
KTLKSISKTK IQVSLKTLEV LKESSKDENG EAQTNDDFEI LSRLQEQNTK
710 720 730 740 750
KLRKRLIRYK RLIKQKLEYR QTVLLNKLIE DETQATTNNT VEKDNNTLER
760 770 780 790 800
LELAQELTML QLQRKNKLSS LVKKFEDNAK IHKYRRIIRE GTEMNIEEVD
810 820 830
SSLDVILQTL IANNNKNKGA EQIITISNAN SHA
Length:833
Mass (Da):93,908
Last modified:February 1, 1995 - v1
Checksum:iBB7C35E29802BBD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74158 Genomic DNA. Translation: CAA52271.1.
Z74104 Genomic DNA. Translation: CAA98618.1.
U19608 Genomic DNA. Translation: AAC49290.1.
BK006938 Genomic DNA. Translation: DAA11800.1.
PIRiA47528.
RefSeqiNP_010227.1. NM_001180115.1.

Genome annotation databases

EnsemblFungiiYDL056W; YDL056W; YDL056W.
GeneIDi851503.
KEGGisce:YDL056W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74158 Genomic DNA. Translation: CAA52271.1.
Z74104 Genomic DNA. Translation: CAA98618.1.
U19608 Genomic DNA. Translation: AAC49290.1.
BK006938 Genomic DNA. Translation: DAA11800.1.
PIRiA47528.
RefSeqiNP_010227.1. NM_001180115.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BM8X-ray1.71A4-102[»]
1L3GNMR-A2-124[»]
1MB1X-ray2.10A1-124[»]
ProteinModelPortaliP39678.
SMRiP39678.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32002. 95 interactors.
DIPiDIP-2391N.
IntActiP39678. 28 interactors.
MINTiMINT-616665.

PTM databases

iPTMnetiP39678.

Proteomic databases

MaxQBiP39678.
PRIDEiP39678.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL056W; YDL056W; YDL056W.
GeneIDi851503.
KEGGisce:YDL056W.

Organism-specific databases

EuPathDBiFungiDB:YDL056W.
SGDiS000002214. MBP1.

Phylogenomic databases

GeneTreeiENSGT00530000067565.
HOGENOMiHOG000113493.
InParanoidiP39678.
KOiK06647.
OMAiGFGKYQG.
OrthoDBiEOG092C16YL.

Enzyme and pathway databases

BioCyciYEAST:G3O-29472-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP39678.
PROiP39678.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
3.10.260.10. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR018004. KilA_N/APSES_HTH.
IPR029793. Mbp1/Res1/Res2.
IPR003163. Tscrpt_reg_HTH_APSES-type.
[Graphical view]
PANTHERiPTHR11707:SF30. PTHR11707:SF30. 2 hits.
PfamiPF04383. KilA-N. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 3 hits.
SM01252. KilA-N. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF54616. SSF54616. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS51299. HTH_APSES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMBP1_YEAST
AccessioniPrimary (citable) accession number: P39678
Secondary accession number(s): D6VRU0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 171 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 521 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.