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P39677 (RRF2M_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosome-releasing factor 2, mitochondrial

Short name=RRF2mt
Alternative name(s):
Elongation factor G 2, mitochondrial
Short name=EF-G2mt
Short name=mEF-G 2
Gene names
Name:MEF2
Ordered Locus Names:YJL102W
ORF Names:D819, J0826
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length819 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Not involved in the GTP-dependent ribosomal translocation step during translation elongation By similarity. Required for respiratory growth and essential for mitochondrial genome maintenance. Ref.8

Subcellular location

Mitochondrion Ref.5 Ref.7.

Disruption phenotype

Results in loss of mitochondrial DNA. Causes a significant slow growth phenotype under normal growth conditions (glucose) and a reduced chronological life-span (CLS). Growth on a non-fermentable carbon source (gylcerol) is absent. Ref.8

Miscellaneous

Present with 1733 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3030Mitochondrion Potential
Chain31 – 819789Ribosome-releasing factor 2, mitochondrial HAMAP-Rule MF_03059
PRO_0000007453

Regions

Nucleotide binding48 – 558GTP By similarity
Nucleotide binding113 – 1175GTP By similarity
Nucleotide binding165 – 1684GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
P39677 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: DDACCD85FC6F6798

FASTA81991,283
        10         20         30         40         50         60 
MWKWNVRRWA GARVNISKNR LSVINVGSRY LSTARSPLSK VRNIGIIAHI DAGKTTTTER 

        70         80         90        100        110        120 
MLYYAGISKH IGDVDTGDTI TDFLEQERSR GITIQSAAIS FPWRNTFAIN LIDTPGHIDF 

       130        140        150        160        170        180 
TFEVIRALKV IDSCVVILDA VAGVEAQTEK VWKQSKSKPK ICFINKMDRM GASFNHTVND 

       190        200        210        220        230        240 
LINKFMRGTT TKPVLVNIPY YRKQPTSNDY VFQGVIDVVN GKRLTWNPEN PDEIIVDELD 

       250        260        270        280        290        300 
GTSLEQCNRC RESMIETLTE YDEDLVQHFL EEAEGDYSKV SAQFLNASIR KLTMKNMIVP 

       310        320        330        340        350        360 
VLCGASFKNI GVQPLLDAIV NYLPSPIEAE LPELNDKTVP MKYDPKVGCL VNNNKNLCIA 

       370        380        390        400        410        420 
LAFKVITDPI RGKQIFIRIY SGTLNSGNTV YNSTTGEKFK LGKLLIPHAG TSQPVNILTA 

       430        440        450        460        470        480 
GQIGLLTGST VENNISTGDT LITHSSKKDG LKSLDKKKEL TLKINSIFIP PPVFGVSIEP 

       490        500        510        520        530        540 
RTLSNKKSME EALNTLITED PSLSISQNDE TGQTVLNGMG ELHLEIAKDR LVNDLKADVE 

       550        560        570        580        590        600 
FGQLMVSYKE TINSETNIET YESDDGYRFS LSLLPNSDAL PNCLAYPLGV NENFLIMEKN 

       610        620        630        640        650        660 
GNWDKEWKYQ VSFESILNSI IASCIVGLQR GGKIANFPLY ACSIKINSDW SVPPDIETPQ 

       670        680        690        700        710        720 
EILKITRNLI FKALNDLKPE KYNLLEPIMN LDLTIPQSDV GTVLQDLTGA RKAQILSIED 

       730        740        750        760        770        780 
ESSVSNSGAS TCNSPENSNR IYIPSDAVTT LHATKDKKNT QETSSNVKKI IKAKVPLREI 

       790        800        810 
TTYTNKLRSL SQGRGEFNIE YSDMEKVTND RLQSILHDL 

« Hide

References

« Hide 'large scale' references
[1]"The yeast nuclear Mef2 gene encodes a second mitochondrial EF-G-like protein."
Welcsh P.L., Johnson D.R., Breitenberger C.A.
Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)."
Rasmussen S.W.
Yeast 11:873-883(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"The proteome of Saccharomyces cerevisiae mitochondria."
Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C.
Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: ATCC 76625 / YPH499.
[8]"The MEF2 gene is essential for yeast longevity, with a dual role in cell respiration and maintenance of mitochondrial membrane potential."
Callegari S., McKinnon R.A., Andrews S., de Barros Lopes M.A.
FEBS Lett. 585:1140-1146(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L25088 Genomic DNA. Translation: AAB59315.1.
X85021 Genomic DNA. Translation: CAA59392.1.
Z49377 Genomic DNA. Translation: CAA89397.1.
BK006943 Genomic DNA. Translation: DAA08698.1.
PIRS43748.
RefSeqNP_012433.1. NM_001181535.1.

3D structure databases

ProteinModelPortalP39677.
SMRP39677. Positions 38-812.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33654. 6 interactions.
DIPDIP-2761N.
IntActP39677. 2 interactions.
MINTMINT-573444.
STRING4932.YJL102W.

Proteomic databases

PaxDbP39677.
PeptideAtlasP39677.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL102W; YJL102W; YJL102W.
GeneID853342.
KEGGsce:YJL102W.

Organism-specific databases

CYGDYJL102w.
SGDS000003638. MEF2.

Phylogenomic databases

eggNOGCOG0480.
GeneTreeENSGT00550000074890.
HOGENOMHOG000231587.
KOK02355.
OMAEMIGYLS.
OrthoDBEOG7NCVCN.

Enzyme and pathway databases

BioCycYEAST:G3O-31556-MONOMER.

Gene expression databases

GenevestigatorP39677.

Family and domain databases

Gene3D3.30.70.240. 2 hits.
3.40.50.300. 1 hit.
HAMAPMF_03059. mEF_G_2.
InterProIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SMARTSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973730.
PROP39677.

Entry information

Entry nameRRF2M_YEAST
AccessionPrimary (citable) accession number: P39677
Secondary accession number(s): D6VW82
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families