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P39677

- RRF2M_YEAST

UniProt

P39677 - RRF2M_YEAST

Protein

Ribosome-releasing factor 2, mitochondrial

Gene

MEF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Not involved in the GTP-dependent ribosomal translocation step during translation elongation By similarity. Required for respiratory growth and essential for mitochondrial genome maintenance.1 PublicationUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi48 – 558GTPUniRule annotation
    Nucleotide bindingi113 – 1175GTPUniRule annotation
    Nucleotide bindingi165 – 1684GTPUniRule annotation

    GO - Molecular functioni

    1. GTPase activity Source: SGD
    2. GTP binding Source: UniProtKB-KW

    GO - Biological processi

    1. GTP catabolic process Source: GOC
    2. mitochondrial genome maintenance Source: SGD
    3. mitochondrial translation Source: SGD
    4. regulation of mitochondrial membrane potential Source: SGD
    5. ribosome disassembly Source: SGD

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31556-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosome-releasing factor 2, mitochondrialUniRule annotation
    Short name:
    RRF2mtUniRule annotation
    Alternative name(s):
    Elongation factor G 2, mitochondrialUniRule annotation
    Short name:
    EF-G2mtUniRule annotation
    Short name:
    mEF-G 2UniRule annotation
    Gene namesi
    Name:MEF2UniRule annotation
    Ordered Locus Names:YJL102W
    ORF Names:D819, J0826
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL102w.
    SGDiS000003638. MEF2.

    Subcellular locationi

    Mitochondrion 2 PublicationsUniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Results in loss of mitochondrial DNA. Causes a significant slow growth phenotype under normal growth conditions (glucose) and a reduced chronological life-span (CLS). Growth on a non-fermentable carbon source (gylcerol) is absent.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3030MitochondrionUniRule annotationAdd
    BLAST
    Chaini31 – 819789Ribosome-releasing factor 2, mitochondrialPRO_0000007453Add
    BLAST

    Proteomic databases

    MaxQBiP39677.
    PaxDbiP39677.
    PeptideAtlasiP39677.

    Expressioni

    Gene expression databases

    GenevestigatoriP39677.

    Interactioni

    Protein-protein interaction databases

    BioGridi33654. 6 interactions.
    DIPiDIP-2761N.
    IntActiP39677. 2 interactions.
    MINTiMINT-573444.
    STRINGi4932.YJL102W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39677.
    SMRiP39677. Positions 38-812.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 327289tr-type GAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0480.
    GeneTreeiENSGT00550000074890.
    HOGENOMiHOG000231587.
    KOiK02355.
    OMAiEMIGYLS.
    OrthoDBiEOG7NCVCN.

    Family and domain databases

    Gene3Di3.30.70.240. 2 hits.
    3.40.50.300. 1 hit.
    HAMAPiMF_03059. mEF_G_2.
    InterProiIPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00679. EFG_C. 1 hit.
    PF14492. EFG_II. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SMARTiSM00838. EFG_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF50447. SSF50447. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P39677-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWKWNVRRWA GARVNISKNR LSVINVGSRY LSTARSPLSK VRNIGIIAHI    50
    DAGKTTTTER MLYYAGISKH IGDVDTGDTI TDFLEQERSR GITIQSAAIS 100
    FPWRNTFAIN LIDTPGHIDF TFEVIRALKV IDSCVVILDA VAGVEAQTEK 150
    VWKQSKSKPK ICFINKMDRM GASFNHTVND LINKFMRGTT TKPVLVNIPY 200
    YRKQPTSNDY VFQGVIDVVN GKRLTWNPEN PDEIIVDELD GTSLEQCNRC 250
    RESMIETLTE YDEDLVQHFL EEAEGDYSKV SAQFLNASIR KLTMKNMIVP 300
    VLCGASFKNI GVQPLLDAIV NYLPSPIEAE LPELNDKTVP MKYDPKVGCL 350
    VNNNKNLCIA LAFKVITDPI RGKQIFIRIY SGTLNSGNTV YNSTTGEKFK 400
    LGKLLIPHAG TSQPVNILTA GQIGLLTGST VENNISTGDT LITHSSKKDG 450
    LKSLDKKKEL TLKINSIFIP PPVFGVSIEP RTLSNKKSME EALNTLITED 500
    PSLSISQNDE TGQTVLNGMG ELHLEIAKDR LVNDLKADVE FGQLMVSYKE 550
    TINSETNIET YESDDGYRFS LSLLPNSDAL PNCLAYPLGV NENFLIMEKN 600
    GNWDKEWKYQ VSFESILNSI IASCIVGLQR GGKIANFPLY ACSIKINSDW 650
    SVPPDIETPQ EILKITRNLI FKALNDLKPE KYNLLEPIMN LDLTIPQSDV 700
    GTVLQDLTGA RKAQILSIED ESSVSNSGAS TCNSPENSNR IYIPSDAVTT 750
    LHATKDKKNT QETSSNVKKI IKAKVPLREI TTYTNKLRSL SQGRGEFNIE 800
    YSDMEKVTND RLQSILHDL 819
    Length:819
    Mass (Da):91,283
    Last modified:February 1, 1995 - v1
    Checksum:iDDACCD85FC6F6798
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L25088 Genomic DNA. Translation: AAB59315.1.
    X85021 Genomic DNA. Translation: CAA59392.1.
    Z49377 Genomic DNA. Translation: CAA89397.1.
    BK006943 Genomic DNA. Translation: DAA08698.1.
    PIRiS43748.
    RefSeqiNP_012433.1. NM_001181535.1.

    Genome annotation databases

    EnsemblFungiiYJL102W; YJL102W; YJL102W.
    GeneIDi853342.
    KEGGisce:YJL102W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L25088 Genomic DNA. Translation: AAB59315.1 .
    X85021 Genomic DNA. Translation: CAA59392.1 .
    Z49377 Genomic DNA. Translation: CAA89397.1 .
    BK006943 Genomic DNA. Translation: DAA08698.1 .
    PIRi S43748.
    RefSeqi NP_012433.1. NM_001181535.1.

    3D structure databases

    ProteinModelPortali P39677.
    SMRi P39677. Positions 38-812.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33654. 6 interactions.
    DIPi DIP-2761N.
    IntActi P39677. 2 interactions.
    MINTi MINT-573444.
    STRINGi 4932.YJL102W.

    Proteomic databases

    MaxQBi P39677.
    PaxDbi P39677.
    PeptideAtlasi P39677.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL102W ; YJL102W ; YJL102W .
    GeneIDi 853342.
    KEGGi sce:YJL102W.

    Organism-specific databases

    CYGDi YJL102w.
    SGDi S000003638. MEF2.

    Phylogenomic databases

    eggNOGi COG0480.
    GeneTreei ENSGT00550000074890.
    HOGENOMi HOG000231587.
    KOi K02355.
    OMAi EMIGYLS.
    OrthoDBi EOG7NCVCN.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31556-MONOMER.

    Miscellaneous databases

    NextBioi 973730.
    PROi P39677.

    Gene expression databases

    Genevestigatori P39677.

    Family and domain databases

    Gene3Di 3.30.70.240. 2 hits.
    3.40.50.300. 1 hit.
    HAMAPi MF_03059. mEF_G_2.
    InterProi IPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR009000. Transl_B-barrel.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00679. EFG_C. 1 hit.
    PF14492. EFG_II. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SMARTi SM00838. EFG_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50447. SSF50447. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast nuclear Mef2 gene encodes a second mitochondrial EF-G-like protein."
      Welcsh P.L., Johnson D.R., Breitenberger C.A.
      Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)."
      Rasmussen S.W.
      Yeast 11:873-883(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: ATCC 76625 / YPH499.
    8. "The MEF2 gene is essential for yeast longevity, with a dual role in cell respiration and maintenance of mitochondrial membrane potential."
      Callegari S., McKinnon R.A., Andrews S., de Barros Lopes M.A.
      FEBS Lett. 585:1140-1146(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiRRF2M_YEAST
    AccessioniPrimary (citable) accession number: P39677
    Secondary accession number(s): D6VW82
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 118 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1733 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3