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P39677

- RRF2M_YEAST

UniProt

P39677 - RRF2M_YEAST

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Protein
Ribosome-releasing factor 2, mitochondrial
Gene
MEF2, YJL102W, D819, J0826
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Not involved in the GTP-dependent ribosomal translocation step during translation elongation By similarity. Required for respiratory growth and essential for mitochondrial genome maintenance.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi48 – 558GTP By similarity
Nucleotide bindingi113 – 1175GTP By similarity
Nucleotide bindingi165 – 1684GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW
  2. GTPase activity Source: SGD

GO - Biological processi

  1. GTP catabolic process Source: GOC
  2. mitochondrial genome maintenance Source: SGD
  3. mitochondrial translation Source: SGD
  4. regulation of mitochondrial membrane potential Source: SGD
  5. ribosome disassembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31556-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-releasing factor 2, mitochondrial
Short name:
RRF2mt
Alternative name(s):
Elongation factor G 2, mitochondrial
Short name:
EF-G2mt
Short name:
mEF-G 2
Gene namesi
Name:MEF2
Ordered Locus Names:YJL102W
ORF Names:D819, J0826
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL102w.
SGDiS000003638. MEF2.

Subcellular locationi

Mitochondrion 2 Publications

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Results in loss of mitochondrial DNA. Causes a significant slow growth phenotype under normal growth conditions (glucose) and a reduced chronological life-span (CLS). Growth on a non-fermentable carbon source (gylcerol) is absent.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3030Mitochondrion Reviewed prediction
Add
BLAST
Chaini31 – 819789Ribosome-releasing factor 2, mitochondrialUniRule annotation
PRO_0000007453Add
BLAST

Proteomic databases

MaxQBiP39677.
PaxDbiP39677.
PeptideAtlasiP39677.

Expressioni

Gene expression databases

GenevestigatoriP39677.

Interactioni

Protein-protein interaction databases

BioGridi33654. 6 interactions.
DIPiDIP-2761N.
IntActiP39677. 2 interactions.
MINTiMINT-573444.
STRINGi4932.YJL102W.

Structurei

3D structure databases

ProteinModelPortaliP39677.
SMRiP39677. Positions 38-812.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 327289tr-type G
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0480.
GeneTreeiENSGT00550000074890.
HOGENOMiHOG000231587.
KOiK02355.
OMAiEMIGYLS.
OrthoDBiEOG7NCVCN.

Family and domain databases

Gene3Di3.30.70.240. 2 hits.
3.40.50.300. 1 hit.
HAMAPiMF_03059. mEF_G_2.
InterProiIPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P39677-1 [UniParc]FASTAAdd to Basket

« Hide

MWKWNVRRWA GARVNISKNR LSVINVGSRY LSTARSPLSK VRNIGIIAHI    50
DAGKTTTTER MLYYAGISKH IGDVDTGDTI TDFLEQERSR GITIQSAAIS 100
FPWRNTFAIN LIDTPGHIDF TFEVIRALKV IDSCVVILDA VAGVEAQTEK 150
VWKQSKSKPK ICFINKMDRM GASFNHTVND LINKFMRGTT TKPVLVNIPY 200
YRKQPTSNDY VFQGVIDVVN GKRLTWNPEN PDEIIVDELD GTSLEQCNRC 250
RESMIETLTE YDEDLVQHFL EEAEGDYSKV SAQFLNASIR KLTMKNMIVP 300
VLCGASFKNI GVQPLLDAIV NYLPSPIEAE LPELNDKTVP MKYDPKVGCL 350
VNNNKNLCIA LAFKVITDPI RGKQIFIRIY SGTLNSGNTV YNSTTGEKFK 400
LGKLLIPHAG TSQPVNILTA GQIGLLTGST VENNISTGDT LITHSSKKDG 450
LKSLDKKKEL TLKINSIFIP PPVFGVSIEP RTLSNKKSME EALNTLITED 500
PSLSISQNDE TGQTVLNGMG ELHLEIAKDR LVNDLKADVE FGQLMVSYKE 550
TINSETNIET YESDDGYRFS LSLLPNSDAL PNCLAYPLGV NENFLIMEKN 600
GNWDKEWKYQ VSFESILNSI IASCIVGLQR GGKIANFPLY ACSIKINSDW 650
SVPPDIETPQ EILKITRNLI FKALNDLKPE KYNLLEPIMN LDLTIPQSDV 700
GTVLQDLTGA RKAQILSIED ESSVSNSGAS TCNSPENSNR IYIPSDAVTT 750
LHATKDKKNT QETSSNVKKI IKAKVPLREI TTYTNKLRSL SQGRGEFNIE 800
YSDMEKVTND RLQSILHDL 819
Length:819
Mass (Da):91,283
Last modified:February 1, 1995 - v1
Checksum:iDDACCD85FC6F6798
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L25088 Genomic DNA. Translation: AAB59315.1.
X85021 Genomic DNA. Translation: CAA59392.1.
Z49377 Genomic DNA. Translation: CAA89397.1.
BK006943 Genomic DNA. Translation: DAA08698.1.
PIRiS43748.
RefSeqiNP_012433.1. NM_001181535.1.

Genome annotation databases

EnsemblFungiiYJL102W; YJL102W; YJL102W.
GeneIDi853342.
KEGGisce:YJL102W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L25088 Genomic DNA. Translation: AAB59315.1 .
X85021 Genomic DNA. Translation: CAA59392.1 .
Z49377 Genomic DNA. Translation: CAA89397.1 .
BK006943 Genomic DNA. Translation: DAA08698.1 .
PIRi S43748.
RefSeqi NP_012433.1. NM_001181535.1.

3D structure databases

ProteinModelPortali P39677.
SMRi P39677. Positions 38-812.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33654. 6 interactions.
DIPi DIP-2761N.
IntActi P39677. 2 interactions.
MINTi MINT-573444.
STRINGi 4932.YJL102W.

Proteomic databases

MaxQBi P39677.
PaxDbi P39677.
PeptideAtlasi P39677.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL102W ; YJL102W ; YJL102W .
GeneIDi 853342.
KEGGi sce:YJL102W.

Organism-specific databases

CYGDi YJL102w.
SGDi S000003638. MEF2.

Phylogenomic databases

eggNOGi COG0480.
GeneTreei ENSGT00550000074890.
HOGENOMi HOG000231587.
KOi K02355.
OMAi EMIGYLS.
OrthoDBi EOG7NCVCN.

Enzyme and pathway databases

BioCyci YEAST:G3O-31556-MONOMER.

Miscellaneous databases

NextBioi 973730.
PROi P39677.

Gene expression databases

Genevestigatori P39677.

Family and domain databases

Gene3Di 3.30.70.240. 2 hits.
3.40.50.300. 1 hit.
HAMAPi MF_03059. mEF_G_2.
InterProi IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
Pfami PF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SMARTi SM00838. EFG_C. 1 hit.
[Graphical view ]
SUPFAMi SSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast nuclear Mef2 gene encodes a second mitochondrial EF-G-like protein."
    Welcsh P.L., Johnson D.R., Breitenberger C.A.
    Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "A 37.5 kb region of yeast chromosome X includes the SME1, MEF2, GSH1 and CSD3 genes, a TCP-1-related gene, an open reading frame similar to the DAL80 gene, and a tRNA(Arg)."
    Rasmussen S.W.
    Yeast 11:873-883(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: ATCC 76625 / YPH499.
  8. "The MEF2 gene is essential for yeast longevity, with a dual role in cell respiration and maintenance of mitochondrial membrane potential."
    Callegari S., McKinnon R.A., Andrews S., de Barros Lopes M.A.
    FEBS Lett. 585:1140-1146(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiRRF2M_YEAST
AccessioniPrimary (citable) accession number: P39677
Secondary accession number(s): D6VW82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1733 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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