Reviewed, UniProtKB/Swiss-Prot P39661 (NIR_SYNE7)

Names and origin

Protein names

Recommended name:
    Ferredoxin--nitrite reductase
    EC=1.7.7.1

Gene names

Name:	nirA
Ordered Locus Names:	Synpcc7942_1240

Organism

Synechococcus elongatus (strain PCC 7942) (Anacystis nidulans R2) [Complete proteome] [HAMAP]

Taxonomic identifier

1140 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	NH3 + 2 H2O + 6 oxidized ferredoxin = nitrite + 6 reduced ferredoxin + 7 H+.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW ferredoxin-nitrite reductase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Ferredoxin--nitrite reductase		PRO_0000199957
Sites
	Metal binding	396	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	402	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	437	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	441	1	Iron (siroheme axial ligand) By similarity
	Metal binding	441	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39661-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 9F469CAF60E816F0	FASTA	512	56,541
	10 20 30 40 50 60 MAQATATTEK LNKFEKLKLE KDGLAVRDQI QHFASIGWEA MDPGDREHRL KWLGIFWRPV 70 80 90 100 110 120 TPGRFMARLR IPSGILQSQQ LNALANFLQR YGDQASIDIT TRQNLQLRGL LLEDTPEFLE 130 140 150 160 170 180 RLHAVGLTSV QSGMDNVRNI TGSPVAGLDA AELFDTRSLI QALQDDLTAA GQGNSEFTNL 190 200 210 220 230 240 PRKFNIAIEG GRDNSIHAEI NDLAFTPAYQ DGTLGFNVWV GGFFSSTRVA PAIPLNAWVP 250 260 270 280 290 300 ADHSVIRLSR AILEVFRDNG SRGNRQKTRL MWLIDEWGIE RFRQVVSEAY GAPLAAAAPE 310 320 330 340 350 360 LMDWEKRDFL GVHPQKQAGL NFVGLHVPVG RLTTEDLYEL ARLADTYGQG EVRLTVEQNV 370 380 390 400 410 420 ILTHIPDAQL PTLLAEPLLT RFSPQPAPLS RGTVSCTGSQ YCNFALIETK QRAIAIAQSL 430 440 450 460 470 480 EAELDLPRPV RIHWTGCPNS CGQPQVADIG LMGAKVRKDG QMVEGVDIFL GGKVGYDAHL 490 500 510 GEKAMTGVAC EDLPDVLRQL LIERFGAQAR SH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Omata T. Submitted (JUL-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Nitrite reductase gene from Synechococcus sp. PCC 7942: homology between cyanobacterial and higher-plant nitrite reductases." Luque I., Flores E., Herrero A. Plant Mol. Biol. 21:1201-1205(1993) [PubMed: 8490140] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete sequence of chromosome 1 of Synechococcus elongatus PCC 7942." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Larimer F., Land M., Kyrpides N., Lykidis A., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Cloning and characterization of the nrtA gene that encodes a 45-kDa protein involved in nitrate transport in the cyanobacterium Synechococcus PCC 7942." Omata T. Plant Cell Physiol. 32:151-157(1991) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 468-512.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12723 Genomic DNA. Translation: BAA02217.1. X67680 Genomic DNA. Translation: CAA47912.1. CP000100 Genomic DNA. Translation: ABB57270.1.
PIR	PQ0646. S33530.
RefSeq	YP_400257.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P39661.
Genome annotation databases
GeneID	3773528.
GenomeReviews	Gene locus Synpcc7942_1240 in contig CP000100_GR.
KEGG	syf:Synpcc7942_1240.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0155.
HOGENOM	HBG578916.
Enzyme and pathway databases
BioCyc	MetaCyc:SYNPCC7942_1240-MONOMER. SELO1140:SYNPCC7942_1240-MONOMER.
Family and domain databases
InterPro	IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Pfam	PF01077. NIR_SIR. 2 hits. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIR_SYNE7

Accession

Primary (citable) accession number: P39661
Secondary accession number(s): Q31NU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 19, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)