Reviewed,
UniProtKB/Swiss-Prot P39656 (OST48_HUMAN)
Last modified
June 16, 2009.
Version 87.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Short name=Oligosaccharyl transferase 48 kDa subunit Short name=DDOST 48 kDa subunit EC=2.4.1.119 | ||||||
| Gene names |
| ||||||
| Organism | Homo sapiens (Human) | ||||||
| Taxonomic identifier | 9606 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 456 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. |
| Catalytic activity | Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine. |
| Pathway | |
| Subunit structure | Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes even after release from the ribosome By similarity. |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass type I membrane protein. |
| Sequence similarities | Belongs to the DDOST 48 kDa subunit family. |
| Caution | It is uncertain whether Met-1 or Met-18 is the initiator. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Membrane |
| Coding sequence diversity | Polymorphism |
| Domain | Signal Transmembrane |
| Molecular function | Transferase |
| Gene Ontology (GO) | |
| Biological process | T cell activation Inferred from direct assay. Source: UniProtKB protein amino acid N-linked glycosylation via asparagineInferred by curator. Source: HGNC protein amino acid terminal N-glycosylationInferred from direct assay. Source: UniProtKB response to cytokine stimulusInferred from direct assay. Source: UniProtKB |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsomeInferred from direct assay. Source: UniProtKB oligosaccharyltransferase complexTraceable author statement. Source: HGNC |
| Molecular function | dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| GSTK1 | Q9Y2Q3 | 1 | EBI-358866,EBI-1053767 | |
| MAGEA6 | P43360 | 1 | EBI-358866,EBI-1045155 | |
| PGRMC1 | O00264 | 1 | EBI-358866,EBI-1045534 | |
| RFXANK | O14593 | 1 | EBI-358866,EBI-1057665 | |
| SAP18 | O00422 | 1 | EBI-358866,EBI-1044156 | |
| TMBIM4 | Q9HC24 | 1 | EBI-358866,EBI-1045545 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 42 | 42 | By similarity | ||||||
| Chain | 43 – 456 | 414 | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit | PRO_0000021957 | |||||
Regions | |||||||||
| Topological domain | 43 – 427 | 385 | Lumenal Potential | ||||||
| Transmembrane | 428 – 447 | 20 | Potential | ||||||
| Topological domain | 448 – 456 | 9 | Cytoplasmic Potential | ||||||
Natural variations | |||||||||
| Natural variant | 8 | 1 | R → G: dbSNP rs537816. | VAR_047911 | |||||
Experimental info | |||||||||
| Sequence conflict | 343 | 1 | G → A in BAA23670. Ref.1 | ||||||
| Sequence conflict | 434 | 1 | S → P in BAA23670. Ref.1 | ||||||
| Sequence conflict | 434 | 1 | S → P in BAA06126. Ref.2 | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Genome organization of human 48-kDa oligosaccharyltransferase (DDOST)." Yamagata T., Tsuru T., Momoi M.Y., Suwa K., Nozaki Y., Mukasa T., Ohashi H., Fukushima Y., Momoi T. Genomics 45:535-540(1997) [PubMed: 9367678] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLY-8. |
| [2] | "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Miyajima N., Tanaka A., Sazuka T., Seki N., Sato S., Tabata S., Ishikawa K., Kawarabayasi Y., Kotani H., Nomura N. DNA Res. 2:37-43(1995) [PubMed: 7788527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-8. Tissue: Bone marrow. |
| [3] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-8. Tissue: Synovium. |
| [4] | "The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. Bentley D.R.Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT GLY-8. |
| [6] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT GLY-8. Tissue: Brain. |
| [7] | "Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients." Shichijo S., Itoh K. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-456. Tissue: Colon adenocarcinoma. |
| [8] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION BY MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| D89060 Genomic DNA. Translation: BAA23670.1. D29643 mRNA. Translation: BAA06126.1. AK315633 mRNA. Translation: BAG38001.1. AL391357 Genomic DNA. Translation: CAH73476.1. CH471134 Genomic DNA. Translation: EAW94938.1. BC002594 mRNA. Translation: AAH02594.1. AB062391 mRNA. Translation: BAB93478.1. Different initiation. | |
| IPI | IPI00297084. |
| PIR | A44654. S66254. |
| RefSeq | NP_005207.2. |
| UniGene | Hs.389171 Hs.523145 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P39656. 16 interactions. |
Genome annotation databases | |
| Ensembl | ENSG00000117242. Homo sapiens. [Contig view] |
| GeneID | 1650. |
| KEGG | hsa:1650. |
Organism-specific databases | |
| GeneCards | GC01M020841. |
| H-InvDB | HIX0000219. |
| HGNC | HGNC:2728. DDOST. |
| MIM | 602202. gene. |
| PharmGKB | PA27195. |
| HUGE | Search... |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOVERGEN | P39656. |
Enzyme and pathway databases | |
| BRENDA | 2.4.1.119. 247. |
Gene expression databases | |
| Bgee | P39656. |
| CleanEx | HS_DDOST. |
| GermOnline | ENSG00000117242. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR005013. DDOST_48kDa. [Graphical view] |
| PANTHER | PTHR10830. DDOST_48kDa. 1 hit. |
| Pfam | PF03345. DDOST_48kD. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 6796. |
| SOURCE | Search... |
Entry information
| Entry name | OST48_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P39656 Secondary accession number(s): B2RDQ4 Q9BUI2 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


