Reviewed, UniProtKB/Swiss-Prot P39656 (OST48_HUMAN)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
      Short name=Oligosaccharyl transferase 48 kDa subunit
      Short name=DDOST 48 kDa subunit
    EC=2.4.1.119

Gene names

Name:	DDOST
Synonyms:	KIAA0115, OST48
ORF Names:	OK/SW-cl.45

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyl transferase (OST) complex By similarity. OST seems to exist in different forms which contain at least RPN1/ribophorin I, RPN2/ribophorin II, OST48, DAD1, and either STT3A or STT3B.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the DDOST 48 kDa subunit family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane
Molecular function	Transferase
Gene Ontology (GO)
Biological process	T cell activation Inferred from direct assay. Source: UniProtKB protein amino acid N-linked glycosylation via asparagine Ref.5 Inferred by curator. Source: HGNC protein amino acid terminal N-glycosylation Inferred from direct assay. Source: UniProtKB response to cytokine stimulus Inferred from direct assay. Source: UniProtKB
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from direct assay. Source: UniProtKB oligosaccharyltransferase complex Ref.5 Traceable author statement. Source: HGNC
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Ref.5 Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GSTK1	Q9Y2Q3	1	EBI-358866,EBI-1053767
MAGEA6	P43360	1	EBI-358866,EBI-1045155
PGRMC1	O00264	1	EBI-358866,EBI-1045534
RFXANK	O14593	1	EBI-358866,EBI-1057665
SAP18	O00422	1	EBI-358866,EBI-1044156
TMBIM4	Q9HC24	1	EBI-358866,EBI-1045545

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	By similarity
	Chain	27 – 439	413	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit		PRO_0000021957
Regions
	Topological domain	27 – 410	384	Lumenal Potential
	Transmembrane	411 – 430	20	Potential
	Topological domain	431 – 439	9	Cytoplasmic Potential
Experimental info
	Sequence conflict	326	1	G → A in BAA23670. Ref.2
	Sequence conflict	417	1	P → S Ref.3 Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P39656-1 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: 32EB6766B6F65ACD	FASTA	439	48,810
	10 20 30 40 50 60 MEPSTAARAW ALFWLLLPLL GAVCASGPRT LVLLDNLNVR ETHSLFFRSL KDRGFELTFK 70 80 90 100 110 120 TADDPSLSLI KYGEFLYDNL IIFSPSVEDF GGNINVETIS AFIDGGGSVL VAASSDIGDP 130 140 150 160 170 180 LRELGSECGI EFDEEKTAVI DHHNYDISDL GQHTLIVADT ENLLKAPTIV GKSSLNPILF 190 200 210 220 230 240 RGVGMVADPD NPLVLDILTG SSTSYSFFPD KPITQYPHAV GKNTLLIAGL QARNNARVIF 250 260 270 280 290 300 SGSLDFFSDS FFNSAVQKAA PGSQRYSQTG NYELAVALSR WVFKEEGVLR VGPVSHHRVG 310 320 330 340 350 360 ETAPPNAYTV TDLVEYSIVI QQLSNGKWVP FDGDDIQLEF VRIDPFVRTF LKKKGGKYSV 370 380 390 400 410 420 QFKLPDVYGV FQFKVDYNRL GYTHLYSSTQ VSVRPLQHTQ YERFIPSAYP YYASAFPMML 430 GLFIFSIVFL HMKEKEKSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Miyajima N., Tanaka A., Sazuka T., Seki N., Sato S., Tabata S., Ishikawa K., Kawarabayasi Y., Kotani H., Nomura N. DNA Res. 2:37-43(1995) [PubMed: 7788527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow.
[2]	"Genome organization of human 48-kDa oligosaccharyltransferase (DDOST)." Yamagata T., Tsuru T., Momoi M.Y., Suwa K., Nozaki Y., Mukasa T., Ohashi H., Fukushima Y., Momoi T. Genomics 45:535-540(1997) [PubMed: 9367678] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients." Shichijo S., Itoh K. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon adenocarcinoma.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits." Shibatani T., David L.L., McCormack A.L., Frueh K., Skach W.R. Biochemistry 44:5982-5992(2005) [PubMed: 15835887] [Abstract] Cited for: COMPOSITION OF THE OLIGOSACCHARYL TRANSFERASE COMPLEX.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D29643 mRNA. Translation: BAA06126.1. Different initiation. D89060 Genomic DNA. Translation: BAA23670.1. Different initiation. AB062391 mRNA. Translation: BAB93478.1. BC002594 mRNA. Translation: AAH02594.1. Different initiation.
PIR	A44654. S66254.
RefSeq	NP_005207.2.
UniGene	Hs.523145
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P39656.
Genome annotation databases
Ensembl	ENSG00000117242. Homo sapiens. [Contig view]
GeneID	1650.
KEGG	hsa:1650.
Organism-specific databases
H-InvDB	HIX0000219.
HGNC	HGNC:2728. DDOST.
HPA	CAB009746.
MIM	602202. gene.
PharmGKB	PA27195.
HUGE	Search...
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	P39656.
Gene expression databases
CleanEx	HS_DDOST.
GermOnline	ENSG00000117242. Homo sapiens.
Family and domain databases
InterPro	IPR005013. DDOST_48kDa. [Graphical view]
PANTHER	PTHR10830. DDOST_48kDa. 1 hit.
Pfam	PF03345. DDOST_48kD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	6796.
SOURCE	Search...

Entry information

Entry name

OST48_HUMAN

Accession

Primary (citable) accession number: P39656
Secondary accession number(s): O43244, Q8NI93, Q9BUI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	April 13, 2004
Last modified:	November 25, 2008
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)