Reviewed,
UniProtKB/Swiss-Prot P39656 (OST48_HUMAN)
Last modified
November 25, 2008.
Version 81.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Short name=Oligosaccharyl transferase 48 kDa subunit Short name=DDOST 48 kDa subunit EC=2.4.1.119 | ||||||
| Gene names |
| ||||||
| Organism | Homo sapiens (Human) | ||||||
| Taxonomic identifier | 9606 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 439 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. |
| Catalytic activity | Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine. |
| Pathway | |
| Subunit structure | Component of the oligosaccharyl transferase (OST) complex By similarity. OST seems to exist in different forms which contain at least RPN1/ribophorin I, RPN2/ribophorin II, OST48, DAD1, and either STT3A or STT3B. |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass type I membrane protein. |
| Sequence similarities | Belongs to the DDOST 48 kDa subunit family. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Membrane |
| Domain | Signal Transmembrane |
| Molecular function | Transferase |
Gene Ontology (GO) | |
| Biological process | T cell activation Inferred from direct assay. Source: UniProtKB protein amino acid N-linked glycosylation via asparagine Ref.5Inferred by curator. Source: HGNC protein amino acid terminal N-glycosylationInferred from direct assay. Source: UniProtKB response to cytokine stimulusInferred from direct assay. Source: UniProtKB |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsomeInferred from direct assay. Source: UniProtKB oligosaccharyltransferase complex Ref.5Traceable author statement. Source: HGNC |
| Molecular function | dolichyl-diphosphooligosaccharide-protein glycotransferase activity Ref.5 Inferred from electronic annotation. Source: InterPro protein bindingInferred from physical interaction. Source: IntAct |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| GSTK1 | Q9Y2Q3 | 1 | EBI-358866,EBI-1053767 | |
| MAGEA6 | P43360 | 1 | EBI-358866,EBI-1045155 | |
| PGRMC1 | O00264 | 1 | EBI-358866,EBI-1045534 | |
| RFXANK | O14593 | 1 | EBI-358866,EBI-1057665 | |
| SAP18 | O00422 | 1 | EBI-358866,EBI-1044156 | |
| TMBIM4 | Q9HC24 | 1 | EBI-358866,EBI-1045545 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 26 | 26 | By similarity | ||||||
| Chain | 27 – 439 | 413 | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit | PRO_0000021957 | |||||
Regions | |||||||||
| Topological domain | 27 – 410 | 384 | Lumenal Potential | ||||||
| Transmembrane | 411 – 430 | 20 | Potential | ||||||
| Topological domain | 431 – 439 | 9 | Cytoplasmic Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 326 | 1 | G → A in BAA23670. Ref.2 | ||||||
| Sequence conflict | 417 | 1 | P → S Ref.3 Ref.4 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1." Nagase T., Miyajima N., Tanaka A., Sazuka T., Seki N., Sato S., Tabata S., Ishikawa K., Kawarabayasi Y., Kotani H., Nomura N. DNA Res. 2:37-43(1995) [PubMed: 7788527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow. |
| [2] | "Genome organization of human 48-kDa oligosaccharyltransferase (DDOST)." Yamagata T., Tsuru T., Momoi M.Y., Suwa K., Nozaki Y., Mukasa T., Ohashi H., Fukushima Y., Momoi T. Genomics 45:535-540(1997) [PubMed: 9367678] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients." Shichijo S., Itoh K. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Colon adenocarcinoma. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. |
| [5] | "Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits." Shibatani T., David L.L., McCormack A.L., Frueh K., Skach W.R. Biochemistry 44:5982-5992(2005) [PubMed: 15835887] [Abstract] Cited for: COMPOSITION OF THE OLIGOSACCHARYL TRANSFERASE COMPLEX. |
Cross-references
Sequence databases | |
|---|---|
| D29643 mRNA. Translation: BAA06126.1. Different initiation. D89060 Genomic DNA. Translation: BAA23670.1. Different initiation. AB062391 mRNA. Translation: BAB93478.1. BC002594 mRNA. Translation: AAH02594.1. Different initiation. | |
| PIR | A44654. S66254. |
| RefSeq | NP_005207.2. |
| UniGene | Hs.523145 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P39656. |
Genome annotation databases | |
| Ensembl | ENSG00000117242. Homo sapiens. [Contig view] |
| GeneID | 1650. |
| KEGG | hsa:1650. |
Organism-specific databases | |
| H-InvDB | HIX0000219. |
| HGNC | HGNC:2728. DDOST. |
| HPA | CAB009746. |
| MIM | 602202. gene. |
| PharmGKB | PA27195. |
| HUGE | Search... |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOVERGEN | P39656. |
Gene expression databases | |
| CleanEx | HS_DDOST. |
| GermOnline | ENSG00000117242. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR005013. DDOST_48kDa. [Graphical view] |
| PANTHER | PTHR10830. DDOST_48kDa. 1 hit. |
| Pfam | PF03345. DDOST_48kD. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 6796. |
| SOURCE | Search... |
Entry information
| Entry name | OST48_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P39656 Secondary accession number(s): O43244, Q8NI93, Q9BUI2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 1 Human chromosome 1: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


