Reviewed,
UniProtKB/Swiss-Prot P39655 (LOX12_MOUSE)
Last modified
November 25, 2008.
Version 74.
History...
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Arachidonate 12-lipoxygenase, 12S-type Short name=12-LOX EC=1.13.11.31 Alternative name(s): Platelet-type lipoxygenase 12 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 663 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Oxygenase and 14,15-leukotriene A4 synthase activity. |
| Catalytic activity | Arachidonate + O(2) = (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate. |
| Cofactor | Binds 1 iron ion per subunit. |
| Pathway | |
| Subcellular location | |
| Tissue specificity | Found primarily in platelets and in microsomal and cytosolic fractions of the epidermis. |
| Sequence similarities | Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 663 | 662 | Arachidonate 12-lipoxygenase, 12S-type | PRO_0000220683 | |||||
Regions | |||||||||
| Domain | 2 – 114 | 113 | PLAT | ||||||
| Domain | 115 – 663 | 549 | Lipoxygenase | ||||||
Sites | |||||||||
| Metal binding | 360 | 1 | Iron; catalytic By similarity | ||||||
| Metal binding | 365 | 1 | Iron; catalytic By similarity | ||||||
| Metal binding | 540 | 1 | Iron; catalytic By similarity | ||||||
| Metal binding | 544 | 1 | Iron; catalytic By similarity | ||||||
| Metal binding | 663 | 1 | Iron; via carboxylate; catalytic By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 663 | 1 | I → D, R, K or G: Loss of activity | ||||||
| Mutagenesis | 663 | 1 | I → S or N: Little activity (8-15%) | ||||||
| Mutagenesis | 663 | 1 | I → V: Nearly full activity | ||||||
| Sequence conflict | 3 | 1 | R → A in AAA20659. Ref.1 | ||||||
| Sequence conflict | 112 | 1 | T → Q in AAA20659. Ref.1 | ||||||
| Sequence conflict | 112 | 1 | T → Q in AAB36013. Ref.2 | ||||||
| Sequence conflict | 403 | 1 | S → T in AAA20659. Ref.1 | ||||||
| Sequence conflict | 403 | 1 | S → T in AAB36013. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "cDNA cloning, expression, mutagenesis of C-terminal isoleucine, genomic structure, and chromosomal localizations of murine 12-lipoxygenases." Chen X.-S., Kurre U., Jenkins N.A., Copeland N.G., Funk C.D. J. Biol. Chem. 269:13979-13987(1994) [PubMed: 8188678] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ILE-663. Strain: C57BL/6 and ICR. Tissue: Spleen. |
| [2] | "12-lipoxygenase isoenzymes in mouse skin tumor development." Krieg P., Kinzig A., Ress-Loschke M., Vogel S., Vanlandingham B., Stephan M., Lehmann W.D., Marks F., Furstenberger G. Mol. Carcinog. 14:118-129(1995) [PubMed: 7576099] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Ovary and Vagina. |
| [4] | The mouse genome sequencing consortium Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J. |
| [5] | "12-lipoxygenase in Lewis lung carcinoma cells: molecular identity, intracellular distribution of activity and protein, and Ca(2+)-dependent translocation from cytosol to membranes." Hagmann W., Gao X., Zacharek A., Wojciechowski L.A., Honn K.V. Prostaglandins 49:49-62(1995) [PubMed: 7792391] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 126-218. Tissue: Lung. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U04334 mRNA. Translation: AAA20659.1. S80446 mRNA. Translation: AAB36013.1. AK036898 mRNA. Translation: BAC29629.1. AK087724 mRNA. Translation: BAC39981.1. AL669869 Genomic DNA. Translation: CAI35235.1. S77511 mRNA. Translation: AAB34667.1. | |
| PIR | A54075. |
| RefSeq | NP_031466.2. |
| UniGene | Mm.12286 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1LOX based on UniProtKB P12530. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | P39655. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000000320. Mus musculus. [Contig view] |
| GeneID | 11684. |
| KEGG | mmu:11684. |
Organism-specific databases | |
| MGI | MGI:87998. Alox12. |
Phylogenomic databases | |
| HOGENOM | P39655. |
| HOVERGEN | P39655. |
Gene expression databases | |
| ArrayExpress | P39655. |
| CleanEx | MM_ALOX12. |
| GermOnline | ENSMUSG00000000320. Mus musculus. |
Family and domain databases | |
| InterPro | IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001885. LipOase_mml. [Graphical view] |
| Gene3D | G3DSA:2.60.60.20. Lipase_LipOase. 1 hit. |
| PANTHER | PTHR11771. LipOase. 1 hit. |
| Pfam | PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view] |
| PRINTS | PR00087. LIPOXYGENASE. PR00467. MAMLPOXGNASE. |
| SMART | SM00308. LH2. 1 hit. [Graphical view] |
| PROSITE | PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 279319. |
| SOURCE | Search... |
Entry information
| Entry name | LOX12_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P39655 Secondary accession number(s): Q8BHG4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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