P39655 (LOX12_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 113.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Arachidonate 12-lipoxygenase, 12S-type Short name=12S-LOX Short name=12S-lipoxygenase EC=1.13.11.31 Alternative name(s): Platelet-type lipoxygenase 12 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) [Reference proteome] | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus![]() |
Protein attributes
| Sequence length | 663 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Oxygenase and 14,15-leukotriene A4 synthase activity. |
| Catalytic activity | Arachidonate + O2 = (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate. |
| Cofactor | Binds 1 iron ion per subunit. |
| Pathway | |
| Subcellular location | |
| Tissue specificity | Found primarily in platelets and in microsomal and cytosolic fractions of the epidermis. |
| Sequence similarities | Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 663 | 662 | Arachidonate 12-lipoxygenase, 12S-type | PRO_0000220683 | |||||
Regions | |||||||||
| Domain | 2 – 114 | 113 | PLAT | ||||||
| Domain | 115 – 663 | 549 | Lipoxygenase | ||||||
Sites | |||||||||
| Metal binding | 360 | 1 | Iron; catalytic By similarity | ||||||
| Metal binding | 365 | 1 | Iron; catalytic By similarity | ||||||
| Metal binding | 540 | 1 | Iron; catalytic By similarity | ||||||
| Metal binding | 544 | 1 | Iron; catalytic By similarity | ||||||
| Metal binding | 663 | 1 | Iron; via carboxylate; catalytic By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 663 | 1 | I → D, R, K or G: Loss of activity. Ref.1 | ||||||
| Mutagenesis | 663 | 1 | I → S or N: Little activity (8-15%). Ref.1 | ||||||
| Mutagenesis | 663 | 1 | I → V: Nearly full activity. Ref.1 | ||||||
| Sequence conflict | 3 | 1 | R → A in AAA20659. Ref.1 | ||||||
| Sequence conflict | 112 | 1 | T → Q in AAA20659. Ref.1 | ||||||
| Sequence conflict | 112 | 1 | T → Q in AAB36013. Ref.2 | ||||||
| Sequence conflict | 403 | 1 | S → T in AAA20659. Ref.1 | ||||||
| Sequence conflict | 403 | 1 | S → T in AAB36013. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "cDNA cloning, expression, mutagenesis of C-terminal isoleucine, genomic structure, and chromosomal localizations of murine 12-lipoxygenases." Chen X.-S., Kurre U., Jenkins N.A., Copeland N.G., Funk C.D. J. Biol. Chem. 269:13979-13987(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF ILE-663. Strain: C57BL/6 and ICR. Tissue: Spleen. |
| [2] | "12-lipoxygenase isoenzymes in mouse skin tumor development." Krieg P., Kinzig A., Ress-Loschke M., Vogel S., Vanlandingham B., Stephan M., Lehmann W.D., Marks F., Furstenberger G. Mol. Carcinog. 14:118-129(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Ovary and Vagina. |
| [4] | "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J. |
| [5] | "12-lipoxygenase in Lewis lung carcinoma cells: molecular identity, intracellular distribution of activity and protein, and Ca(2+)-dependent translocation from cytosol to membranes." Hagmann W., Gao X., Zacharek A., Wojciechowski L.A., Honn K.V. Prostaglandins 49:49-62(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 126-218. Tissue: Lung. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U04334 mRNA. Translation: AAA20659.1. S80446 mRNA. Translation: AAB36013.1. AK036898 mRNA. Translation: BAC29629.1. AK087724 mRNA. Translation: BAC39981.1. AL669869 Genomic DNA. Translation: CAI35235.1. S77511 mRNA. Translation: AAB34667.1. |
| IPI | IPI00308268. |
| PIR | A54075. |
| RefSeq | NP_031466.2. NM_007440.4. |
| UniGene | Mm.12286. |
3D structure databases | |
| ProteinModelPortal | P39655. |
| SMR | P39655. Positions 2-663. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | P39655. |
Proteomic databases | |
| PaxDb | P39655. |
| PRIDE | P39655. |
Protocols and materials databases | |
| DNASU | 11684. |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000000329; ENSMUSP00000000329; ENSMUSG00000000320. |
| GeneID | 11684. |
| KEGG | mmu:11684. |
Organism-specific databases | |
| CTD | 239. |
| MGI | MGI:87998. Alox12. |
Phylogenomic databases | |
| eggNOG | NOG69653. |
| GeneTree | ENSGT00550000074415. |
| HOGENOM | HOG000234358. |
| HOVERGEN | HBG005150. |
| InParanoid | P39655. |
| KO | K00458. |
| OMA | HHKEKYF. |
| OrthoDB | EOG4W0XCM. |
Enzyme and pathway databases | |
| UniPathway | UPA00880. |
Gene expression databases | |
| ArrayExpress | P39655. |
| Bgee | P39655. |
| CleanEx | MM_ALOX12. |
| Genevestigator | P39655. |
| GermOnline | ENSMUSG00000000320. Mus musculus. |
Family and domain databases | |
| Gene3D | 2.60.60.20. 1 hit. |
| InterPro | IPR008976. Lipase_LipOase. IPR000907. LipOase. IPR013819. LipOase_C. IPR020834. LipOase_CS. IPR020833. LipOase_Fe_BS. IPR001024. LipOase_LH2. IPR001885. LipOase_mml. [Graphical view] |
| PANTHER | PTHR11771. PTHR11771. 1 hit. |
| Pfam | PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view] |
| PRINTS | PR00087. LIPOXYGENASE. PR00467. MAMLPOXGNASE. |
| SMART | SM00308. LH2. 1 hit. [Graphical view] |
| SUPFAM | SSF49723. Lipase_LipOase. 1 hit. SSF48484. Lipoxygenase. 1 hit. |
| PROSITE | PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| BindingDB | P39655. |
| ChEMBL | CHEMBL3225. |
| NextBio | 279319. |
| SOURCE | Search... |
Entry information
| Entry name | LOX12_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P39655 Secondary accession number(s): Q8BHG4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
