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P39653

- DEXT_STRDO

UniProt

P39653 - DEXT_STRDO

Protein

Dextranase

Gene

dex

Organism
Streptococcus downei (Streptococcus sobrinus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    May play a role in sucrose-independent adherence to the pellicle-coated tooth surface.

    Catalytic activityi

    Endohydrolysis of (1->6)-alpha-D-glucosidic linkages in dextran.

    pH dependencei

    Optimum pH is 5.3.

    Temperature dependencei

    Optimum temperature is 39 degrees Celsius.

    GO - Molecular functioni

    1. dextranase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BRENDAi3.2.1.11. 5954.

    Protein family/group databases

    CAZyiGH66. Glycoside Hydrolase Family 66.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dextranase (EC:3.2.1.11)
    Alternative name(s):
    Alpha-1,6-glucan-6-glucanohydrolase
    Gene namesi
    Name:dex
    Encoded oniPlasmid pYA9020 Publication
    OrganismiStreptococcus downei (Streptococcus sobrinus)
    Taxonomic identifieri1317 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

    Subcellular locationi

    Secretedcell wall PROSITE-ProRule annotation; Peptidoglycan-anchor PROSITE-ProRule annotation

    GO - Cellular componenti

    1. cell wall Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 30301 PublicationAdd
    BLAST
    Chaini31 – 13081278DextranasePRO_0000012236Add
    BLAST
    Propeptidei1309 – 133729Removed by sortasePROSITE-ProRule annotationPRO_0000012237Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1308 – 13081Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation

    Keywords - PTMi

    Peptidoglycan-anchor

    Interactioni

    Subunit structurei

    Homodimer.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1305 – 13095LPXTG sorting signal

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 66 family.Curated

    Keywords - Domaini

    Repeat, Signal

    Family and domain databases

    InterProiIPR025092. Glyco_hydro_66.
    IPR019931. LPXTG_anchor.
    [Graphical view]
    PfamiPF13199. Glyco_hydro_66. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01167. LPXTG_anchor. 1 hit.
    PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P39653-1 [UniParc]FASTAAdd to Basket

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    MNNRMLSFPS MLFLLAFGIV LSVSAGTTHA DELANQTAKV ADEASIVVST     50
    SQAAVEQTQS QEKEISPAME EDTSNLSLKP NAQQESQSPD SSTELQDPAE 100
    QTPPETSDAS APATTSADSV EKYAQDATQN QSSTSNGPGV IRATSAQVTA 150
    TRSVVSSQSG DAIVDLSADK ASYRQGEDVN LSVDFKNTTD KEQDVTVYAD 200
    VYYIDNKLGT YKFSKHLKAG EGYKMQSGDL KIPASQFENN HGYLLKVRVR 250
    DADNNTLSEV NKAIAVESDW TKFPRYGIVG GSQDTNNSLL SKDADRYRAE 300
    IEKMKNMNIN SYFFYDVYKT ATNPFPSDEA TFKQDWNTWS GSEIDTQAVK 350
    DIVNQVHDGG AVAMLYNMIL AENTNTGEAP VLPETEYAYN SDDRGYGAQG 400
    QPMSYTVKIP KDGQEEDVEI QRYYNPTSKL WQDYIADKMG QAMKNGGFDG 450
    WQGDTIGDNE VYSYADKDSN DPSKKFWLTE GYAEFLRAIK EKLPNYYLTV 500
    NDVNGEQIYR LKDGNQDVIY NEIWPFGPAL PSEMAAVKPN TVTSRPVLTK 550
    VRQGDWKISI VGAYMEGSEN GGSKADAEAG KSLQTDAVLL TSASIAAAGG 600
    YHMSLAALAN QQDETDGGQG IGVLQTAYYP TQSLKTSSEL TRKNNDYQQF 650
    ITAYENVLRD GVENDDAQVN TFDSNGQKLS TDAKGITGNQ VWTYGKKGDN 700
    FRTVQLLNLM GINSDWKNED GSAANKTPDE QTNLTVKYAL GDVSMEDAQR 750
    MANQTYVTSP DDWSKSNLQK VSASVKTDEN GKPVLVINVP KLTLWDVVYI 800
    SNANQESAPE ADQAQTPAAQ SSDDKVAENE TSQPAAEDAK EQTSEPAQDQ 850
    AAPAEQGQAI NQAESPATEP EAEVTPATAE PAKVDAPEAN QAADQAVSPE 900
    PASQEQAASQ SQPEANQTPA SNETPATQGN SEQPELNEPT AQTQPSSQVS 950
    PANTSVTPVA EQPTNQGQAA DKADQAPTNS TSTPESTSPV EPAATDQSSD 1000
    TPIVTAGNLS VQPAETETPT VPDKQGDSKA NQSSTETPVA DQVPAVAEQP 1050
    QATEPNQAKP SVDKAAAPEA LSLIQLKQQT PAIQAKEADD PEVDETKSEV 1100
    TPDSGTDKAP EAGQVDSDKA PTVKPSTPEN NDNQPNNAND ADKNKTNEAD 1150
    SNKANQDSTK GSSADQSGKS TTPEDGPDNS SPEDPETKPS DPNTDTSDQE 1200
    QVKPSLPVVP NQTVDDPKTD DTDTPANTDS AKSKKVADAD KNKVATDSEG 1250
    RQKSSEFPKE ATDLEKVGQP ASPQVAGVKS SVATSPEKKS EPVSKTSTTS 1300
    SSDKLPKTGD HKTVVLIIVL GLVFVGMTGL LARHEKK 1337
    Length:1,337
    Mass (Da):143,298
    Last modified:February 1, 1995 - v1
    Checksum:iB494275A77A2E3D0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96978 Genomic DNA. Translation: AAA21772.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96978 Genomic DNA. Translation: AAA21772.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH66. Glycoside Hydrolase Family 66.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 3.2.1.11. 5954.

    Family and domain databases

    InterProi IPR025092. Glyco_hydro_66.
    IPR019931. LPXTG_anchor.
    [Graphical view ]
    Pfami PF13199. Glyco_hydro_66. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01167. LPXTG_anchor. 1 hit.
    PROSITEi PS50847. GRAM_POS_ANCHORING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Purification and characterization of Streptococcus sobrinus dextranase produced in recombinant Escherichia coli and sequence analysis of the dextranase gene."
      Wanda S.-Y., Curtiss R. III
      J. Bacteriol. 176:3839-3850(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-36.
      Strain: 6715 / UAB66.

    Entry informationi

    Entry nameiDEXT_STRDO
    AccessioniPrimary (citable) accession number: P39653
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Caution

    It is uncertain whether Met-1 or Met-5 is the initiator.Curated

    Keywords - Technical termi

    Direct protein sequencing, Plasmid

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3