Reviewed, UniProtKB/Swiss-Prot P39653 (DEXT_STRDO)

Names and origin

Protein names

Recommended name:
    Dextranase
    EC=3.2.1.11
Alternative name(s):
    Alpha-1,6-glucan-6-glucanohydrolase

Gene names

Name:

dex

Encoded on

Plasmid pYA902

Organism

Streptococcus downei (Streptococcus sobrinus)

Taxonomic identifier

1317 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	1337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in sucrose-independent adherence to the pellicle-coated tooth surface.
Catalytic activity	Endohydrolysis of (1->6)-alpha-D-glucosidic linkages in dextran.
Subunit structure	Homodimer.
Subcellular location	Secreted › cell wall; Peptidoglycan-anchor Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 66 family.
Caution	It is uncertain whether Met-1 or Met-5 is the initiator.
Biophysicochemical properties	pH dependence: Optimum pH is 5.3. Temperature dependence: Optimum temperature is 39 degrees Celsius.

Ontologies

Keywords
Cellular component	Cell wall Secreted
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Peptidoglycan-anchor
Technical term	Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cell surface Inferred from electronic annotation. Source: InterPro cell wall Inferred from electronic annotation. Source: UniProtKB-KW extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dextranase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Ref.1
	Chain	31 – 1308	1278	Dextranase		PRO_0000012236
	Propeptide	1309 – 1337	29	Removed by sortase Potential		PRO_0000012237
Regions
	Motif	1305 – 1309	5	LPXTG sorting signal Potential
Amino acid modifications
	Modified residue	1308	1	Pentaglycyl murein peptidoglycan amidated threonine Potential

Sequences

	Sequence		Length	Mass (Da)
	P39653-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: B494275A77A2E3D0	FASTA	1,337	143,298
	10 20 30 40 50 60 MNNRMLSFPS MLFLLAFGIV LSVSAGTTHA DELANQTAKV ADEASIVVST SQAAVEQTQS 70 80 90 100 110 120 QEKEISPAME EDTSNLSLKP NAQQESQSPD SSTELQDPAE QTPPETSDAS APATTSADSV 130 140 150 160 170 180 EKYAQDATQN QSSTSNGPGV IRATSAQVTA TRSVVSSQSG DAIVDLSADK ASYRQGEDVN 190 200 210 220 230 240 LSVDFKNTTD KEQDVTVYAD VYYIDNKLGT YKFSKHLKAG EGYKMQSGDL KIPASQFENN 250 260 270 280 290 300 HGYLLKVRVR DADNNTLSEV NKAIAVESDW TKFPRYGIVG GSQDTNNSLL SKDADRYRAE 310 320 330 340 350 360 IEKMKNMNIN SYFFYDVYKT ATNPFPSDEA TFKQDWNTWS GSEIDTQAVK DIVNQVHDGG 370 380 390 400 410 420 AVAMLYNMIL AENTNTGEAP VLPETEYAYN SDDRGYGAQG QPMSYTVKIP KDGQEEDVEI 430 440 450 460 470 480 QRYYNPTSKL WQDYIADKMG QAMKNGGFDG WQGDTIGDNE VYSYADKDSN DPSKKFWLTE 490 500 510 520 530 540 GYAEFLRAIK EKLPNYYLTV NDVNGEQIYR LKDGNQDVIY NEIWPFGPAL PSEMAAVKPN 550 560 570 580 590 600 TVTSRPVLTK VRQGDWKISI VGAYMEGSEN GGSKADAEAG KSLQTDAVLL TSASIAAAGG 610 620 630 640 650 660 YHMSLAALAN QQDETDGGQG IGVLQTAYYP TQSLKTSSEL TRKNNDYQQF ITAYENVLRD 670 680 690 700 710 720 GVENDDAQVN TFDSNGQKLS TDAKGITGNQ VWTYGKKGDN FRTVQLLNLM GINSDWKNED 730 740 750 760 770 780 GSAANKTPDE QTNLTVKYAL GDVSMEDAQR MANQTYVTSP DDWSKSNLQK VSASVKTDEN 790 800 810 820 830 840 GKPVLVINVP KLTLWDVVYI SNANQESAPE ADQAQTPAAQ SSDDKVAENE TSQPAAEDAK 850 860 870 880 890 900 EQTSEPAQDQ AAPAEQGQAI NQAESPATEP EAEVTPATAE PAKVDAPEAN QAADQAVSPE 910 920 930 940 950 960 PASQEQAASQ SQPEANQTPA SNETPATQGN SEQPELNEPT AQTQPSSQVS PANTSVTPVA 970 980 990 1000 1010 1020 EQPTNQGQAA DKADQAPTNS TSTPESTSPV EPAATDQSSD TPIVTAGNLS VQPAETETPT 1030 1040 1050 1060 1070 1080 VPDKQGDSKA NQSSTETPVA DQVPAVAEQP QATEPNQAKP SVDKAAAPEA LSLIQLKQQT 1090 1100 1110 1120 1130 1140 PAIQAKEADD PEVDETKSEV TPDSGTDKAP EAGQVDSDKA PTVKPSTPEN NDNQPNNAND 1150 1160 1170 1180 1190 1200 ADKNKTNEAD SNKANQDSTK GSSADQSGKS TTPEDGPDNS SPEDPETKPS DPNTDTSDQE 1210 1220 1230 1240 1250 1260 QVKPSLPVVP NQTVDDPKTD DTDTPANTDS AKSKKVADAD KNKVATDSEG RQKSSEFPKE 1270 1280 1290 1300 1310 1320 ATDLEKVGQP ASPQVAGVKS SVATSPEKKS EPVSKTSTTS SSDKLPKTGD HKTVVLIIVL 1330 GLVFVGMTGL LARHEKK

References

[1]

"Purification and characterization of Streptococcus sobrinus dextranase produced in recombinant Escherichia coli and sequence analysis of the dextranase gene." Wanda S.-Y., Curtiss R. III J. Bacteriol. 176:3839-3850(1994) [PubMed: 8021165] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 31-36. Strain: 6715 / UAB66.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96978 Genomic DNA. Translation: AAA21772.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH66. Glycoside Hydrolase Family 66.
Enzyme and pathway databases
BRENDA	3.2.1.11. 290403.
Family and domain databases
InterPro	IPR019931. LPXTG-motif_cell_wall_anchor. IPR001899. Surface_protein_Gram_pos_cocci. [Graphical view]
TIGRFAMs	TIGR01167. LPXTG_anchor. 1 hit.
PROSITE	PS50847. GRAM_POS_ANCHORING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DEXT_STRDO

Accession

Primary (citable) accession number: P39653

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)