Reviewed, UniProtKB/Swiss-Prot P39634 (ROCA_BACSU)

Names and originHide

Protein names

Recommended name:
1-pyrroline-5-carboxylate dehydrogenase
Short name=P5C dehydrogenase
EC=1.5.1.12

Gene names

Name:	rocA
Ordered Locus Names:	BSU37780
ORF Names:	ipa-76d

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributesHide

Sequence length	515 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)Hide

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. HAMAP MF_00733
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. HAMAP MF_00733
Induction	Expression is sigma L dependent and induced by arginine. HAMAP MF_00733
Sequence similarities	Belongs to the aldehyde dehydrogenase family. RocA subfamily.

OntologiesHide

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)Hide

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 515	515	1-pyrroline-5-carboxylate dehydrogenase HAMAP MF_00733		PRO_0000056511
Sites
	Active site	286	1	By similarity
	Active site	320	1	By similarity

SequencesHide

	Sequence		Length	Mass (Da)
	P39634-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: C8115B4AEB4C979F	FASTA	515	56,320
	10 20 30 40 50 60 MTVTYAHEPF TDFTEAKNKT AFGESLAFVN TQLGKHYPLV INGEKIETDR KIISINPANK 70 80 90 100 110 120 EEIIGYASTA DQELAEKAMQ AALQAFDSWK KQRPEHRANI LFKAAAILRR RKHEFSSYLV 130 140 150 160 170 180 KEAGKPWKEA DADTAEAIDF LEFYARQMLK LKEGAPVKSR AGEVNQYHYE ALGVGIVISP 190 200 210 220 230 240 FNFPLAIMAG TAVAAIVTGN TILLKPADAA PVVAAKFVEV MEEAGLPNGV LNYIPGDGAE 250 260 270 280 290 300 IGDFLVEHPK TRFVSFTGSR AVGCRIYERA AKVQPGQKWL KRVIAEMGGK DTVLVDKDAD 310 320 330 340 350 360 LDLAASSIVY SAFGYSGQKC SAGSRAVIHQ DVYDEVVEKA VALTKTLTVG NPEDPDTYMG 370 380 390 400 410 420 PVIHEASYNK VMKYIEIGKS EGKLLAGGEG DDSKGYFIQP TIFADVDENA RLMQEEIFGP 430 440 450 460 470 480 VVAICKARDF DHMLEIANNT EYGLTGALLT KNRAHIERAR EDFHVGNLYF NRGCTGAIVG 490 500 510 YQPFGGFNMS GTDSKAGGPD YLILHMQAKT TSEAF

ReferencesHide

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325 degrees to 333 degrees." Glaser P., Kunst F., Arnaud M., Coudart M.P., Gonzales W., Hullo M.-F., Ionescu M., Lubochinsky B., Marcelino L., Moszer I., Presecan E., Santana M., Schneider E., Schweizer J., Vertes A., Rapoport G., Danchin A. Mol. Microbiol. 10:371-384(1993) [PubMed: 7934828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-referencesHide

Sequence databases
EMBL GenBank DDBJ	X73124 Genomic DNA. Translation: CAA51632.1. AL009126 Genomic DNA. Translation: CAB15805.1.
PIR	S39731.
RefSeq	NP_391658.1.
3D structure databases
SMR	P39634. Positions 4-515.
ModBase	Search...
PTM databases
PhosSite	P39634.
Genome annotation databases
EnsemblBacteria	EBBACT00000001608; EBBACP00000001608; EBBACG00000001606.
GeneID	937222.
GenomeReviews	Gene locus BSU37780 in contig AL009126_GR.
KEGG	bsu:BSU37780.
NMPDR	fig|224308.1.peg.3784.
Organism-specific databases
GenoList	BSU37780. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	HBG752218.
OMA	INERASK.
PhylomeDB	P39634.
ProtClustDB	PRK03137.
Enzyme and pathway databases
BRENDA	1.5.1.12. 150.
Family and domain databases
HAMAP	MF_00733. RocA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR005932. d-1-pyrroline-5-COlate_DH-2. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR01237. D1pyr5carbox2. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry informationHide

Entry name

ROCA_BACSU

Accession

Primary (citable) accession number: P39634

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	July 13, 2010
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)