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Protein

Uracil-DNA glycosylase

Gene

ung

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.By similarity

Catalytic activityi

Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Proton acceptorBy similarity

GO - Molecular functioni

  1. uracil DNA N-glycosylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. base-excision repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciBSUB:BSU37970-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil-DNA glycosylase (EC:3.2.2.27)
Short name:
UDG
Gene namesi
Name:ung
Synonyms:ywdG
Ordered Locus Names:BSU37970
ORF Names:ipa-57d
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU37970. [Micado]

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225Uracil-DNA glycosylasePRO_0000176067Add
BLAST

Proteomic databases

PaxDbiP39615.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU37970.

Structurei

Secondary structure

1
225
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 1711Combined sources
Helixi20 – 3516Combined sources
Beta strandi38 – 403Combined sources
Helixi42 – 443Combined sources
Helixi47 – 515Combined sources
Turni54 – 563Combined sources
Beta strandi59 – 613Combined sources
Turni70 – 723Combined sources
Beta strandi75 – 784Combined sources
Helixi88 – 10013Combined sources
Helixi112 – 1165Combined sources
Beta strandi123 – 1253Combined sources
Turni133 – 1386Combined sources
Helixi141 – 15414Combined sources
Beta strandi160 – 1656Combined sources
Helixi166 – 1694Combined sources
Helixi170 – 1745Combined sources
Turni177 – 1793Combined sources
Beta strandi181 – 1855Combined sources
Turni190 – 1923Combined sources
Helixi193 – 1953Combined sources
Turni196 – 1994Combined sources
Helixi202 – 21211Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZOQX-ray1.45A1-225[»]
3ZORX-ray2.95A1-225[»]
ProteinModelPortaliP39615.
SMRiP39615. Positions 1-224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the uracil-DNA glycosylase family.Curated

Phylogenomic databases

eggNOGiCOG0692.
HOGENOMiHOG000229528.
InParanoidiP39615.
KOiK03648.
OMAiEIYPPGP.
OrthoDBiEOG6MSS63.
PhylomeDBiP39615.

Family and domain databases

Gene3Di3.40.470.10. 1 hit.
HAMAPiMF_00148. UDG.
InterProiIPR018085. Ura-DNA_Glyclase_AS.
IPR002043. Ura_DNA_glycsylse.
IPR005122. Uracil-DNA_glycosylase-like.
[Graphical view]
PANTHERiPTHR11264. PTHR11264. 1 hit.
PfamiPF03167. UDG. 1 hit.
[Graphical view]
SMARTiSM00986. UDG. 1 hit.
[Graphical view]
SUPFAMiSSF52141. SSF52141. 1 hit.
TIGRFAMsiTIGR00628. ung. 1 hit.
PROSITEiPS00130. U_DNA_GLYCOSYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39615-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKQLLQDSWW NQLKEEFEKP YYQELREMLK REYAEQTIYP DSRDIFNALH
60 70 80 90 100
YTSYDDVKVV ILGQDPYHGP GQAQGLSFSV KPGVKQPPSL KNIFLELQQD
110 120 130 140 150
IGCSIPNHGS LVSWAKQGVL LLNTVLTVRR GQANSHKGKG WERLTDRIID
160 170 180 190 200
VLSERERPVI FILWGRHAQM KKERIDTSKH FIIESTHPSP FSARNGFFGS
210 220
RPFSRANAYL EKMGEAPIDW CIKDL
Length:225
Mass (Da):26,047
Last modified:February 1, 1995 - v1
Checksum:i649052A9C2904F9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73124 Genomic DNA. Translation: CAA51613.1.
AL009126 Genomic DNA. Translation: CAB15823.1.
PIRiS39712.
RefSeqiNP_391676.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15823; CAB15823; BSU37970.
GeneIDi937265.
KEGGibsu:BSU37970.
PATRICi18979638. VBIBacSub10457_3981.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73124 Genomic DNA. Translation: CAA51613.1.
AL009126 Genomic DNA. Translation: CAB15823.1.
PIRiS39712.
RefSeqiNP_391676.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ZOQX-ray1.45A1-225[»]
3ZORX-ray2.95A1-225[»]
ProteinModelPortaliP39615.
SMRiP39615. Positions 1-224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU37970.

Proteomic databases

PaxDbiP39615.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15823; CAB15823; BSU37970.
GeneIDi937265.
KEGGibsu:BSU37970.
PATRICi18979638. VBIBacSub10457_3981.

Organism-specific databases

GenoListiBSU37970. [Micado]

Phylogenomic databases

eggNOGiCOG0692.
HOGENOMiHOG000229528.
InParanoidiP39615.
KOiK03648.
OMAiEIYPPGP.
OrthoDBiEOG6MSS63.
PhylomeDBiP39615.

Enzyme and pathway databases

BioCyciBSUB:BSU37970-MONOMER.

Family and domain databases

Gene3Di3.40.470.10. 1 hit.
HAMAPiMF_00148. UDG.
InterProiIPR018085. Ura-DNA_Glyclase_AS.
IPR002043. Ura_DNA_glycsylse.
IPR005122. Uracil-DNA_glycosylase-like.
[Graphical view]
PANTHERiPTHR11264. PTHR11264. 1 hit.
PfamiPF03167. UDG. 1 hit.
[Graphical view]
SMARTiSM00986. UDG. 1 hit.
[Graphical view]
SUPFAMiSSF52141. SSF52141. 1 hit.
TIGRFAMsiTIGR00628. ung. 1 hit.
PROSITEiPS00130. U_DNA_GLYCOSYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiUNG_BACSU
AccessioniPrimary (citable) accession number: P39615
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 7, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.