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Protein

Uracil-DNA glycosylase

Gene

ung

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.By similarity

Catalytic activityi

Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Enzyme and pathway databases

BioCyciBSUB:BSU37970-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil-DNA glycosylase (EC:3.2.2.27)
Short name:
UDG
Gene namesi
Name:ung
Synonyms:ywdG
Ordered Locus Names:BSU37970
ORF Names:ipa-57d
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001760671 – 225Uracil-DNA glycosylaseAdd BLAST225

Proteomic databases

PaxDbiP39615.

Interactioni

Subunit structurei

Interacts with bacillus phage phi29 protein p56; this interaction inhibits the uracil-DNA glycosylase.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100020506.

Structurei

Secondary structure

1225
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 17Combined sources11
Helixi20 – 35Combined sources16
Beta strandi38 – 40Combined sources3
Helixi42 – 44Combined sources3
Helixi47 – 51Combined sources5
Turni54 – 56Combined sources3
Beta strandi59 – 61Combined sources3
Turni70 – 72Combined sources3
Beta strandi75 – 78Combined sources4
Helixi88 – 100Combined sources13
Helixi112 – 116Combined sources5
Beta strandi123 – 125Combined sources3
Turni133 – 138Combined sources6
Helixi141 – 154Combined sources14
Beta strandi160 – 165Combined sources6
Helixi166 – 169Combined sources4
Helixi170 – 174Combined sources5
Turni177 – 179Combined sources3
Beta strandi181 – 185Combined sources5
Turni190 – 192Combined sources3
Helixi193 – 195Combined sources3
Turni196 – 199Combined sources4
Helixi202 – 212Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZOQX-ray1.45A1-225[»]
3ZORX-ray2.95A1-225[»]
ProteinModelPortaliP39615.
SMRiP39615.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the uracil-DNA glycosylase family.Curated

Phylogenomic databases

eggNOGiENOG4105D5S. Bacteria.
COG0692. LUCA.
HOGENOMiHOG000229528.
InParanoidiP39615.
KOiK03648.
OMAiIALIPKN.
PhylomeDBiP39615.

Family and domain databases

CDDicd10027. UDG_F1. 1 hit.
Gene3Di3.40.470.10. 1 hit.
HAMAPiMF_00148. UDG. 1 hit.
InterProiIPR002043. UDG_fam1.
IPR018085. Ura-DNA_Glyclase_AS.
IPR005122. Uracil-DNA_glycosylase-like.
[Graphical view]
PANTHERiPTHR11264. PTHR11264. 1 hit.
PfamiPF03167. UDG. 1 hit.
[Graphical view]
SMARTiSM00986. UDG. 1 hit.
[Graphical view]
SUPFAMiSSF52141. SSF52141. 1 hit.
TIGRFAMsiTIGR00628. ung. 1 hit.
PROSITEiPS00130. U_DNA_GLYCOSYLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39615-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQLLQDSWW NQLKEEFEKP YYQELREMLK REYAEQTIYP DSRDIFNALH
60 70 80 90 100
YTSYDDVKVV ILGQDPYHGP GQAQGLSFSV KPGVKQPPSL KNIFLELQQD
110 120 130 140 150
IGCSIPNHGS LVSWAKQGVL LLNTVLTVRR GQANSHKGKG WERLTDRIID
160 170 180 190 200
VLSERERPVI FILWGRHAQM KKERIDTSKH FIIESTHPSP FSARNGFFGS
210 220
RPFSRANAYL EKMGEAPIDW CIKDL
Length:225
Mass (Da):26,047
Last modified:February 1, 1995 - v1
Checksum:i649052A9C2904F9C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73124 Genomic DNA. Translation: CAA51613.1.
AL009126 Genomic DNA. Translation: CAB15823.1.
PIRiS39712.
RefSeqiNP_391676.1. NC_000964.3.
WP_003242965.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB15823; CAB15823; BSU37970.
GeneIDi937265.
KEGGibsu:BSU37970.
PATRICi18979638. VBIBacSub10457_3981.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73124 Genomic DNA. Translation: CAA51613.1.
AL009126 Genomic DNA. Translation: CAB15823.1.
PIRiS39712.
RefSeqiNP_391676.1. NC_000964.3.
WP_003242965.1. NZ_JNCM01000034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ZOQX-ray1.45A1-225[»]
3ZORX-ray2.95A1-225[»]
ProteinModelPortaliP39615.
SMRiP39615.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100020506.

Proteomic databases

PaxDbiP39615.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15823; CAB15823; BSU37970.
GeneIDi937265.
KEGGibsu:BSU37970.
PATRICi18979638. VBIBacSub10457_3981.

Phylogenomic databases

eggNOGiENOG4105D5S. Bacteria.
COG0692. LUCA.
HOGENOMiHOG000229528.
InParanoidiP39615.
KOiK03648.
OMAiIALIPKN.
PhylomeDBiP39615.

Enzyme and pathway databases

BioCyciBSUB:BSU37970-MONOMER.

Family and domain databases

CDDicd10027. UDG_F1. 1 hit.
Gene3Di3.40.470.10. 1 hit.
HAMAPiMF_00148. UDG. 1 hit.
InterProiIPR002043. UDG_fam1.
IPR018085. Ura-DNA_Glyclase_AS.
IPR005122. Uracil-DNA_glycosylase-like.
[Graphical view]
PANTHERiPTHR11264. PTHR11264. 1 hit.
PfamiPF03167. UDG. 1 hit.
[Graphical view]
SMARTiSM00986. UDG. 1 hit.
[Graphical view]
SUPFAMiSSF52141. SSF52141. 1 hit.
TIGRFAMsiTIGR00628. ung. 1 hit.
PROSITEiPS00130. U_DNA_GLYCOSYLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUNG_BACSU
AccessioniPrimary (citable) accession number: P39615
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.