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P39583 (YWAC_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP pyrophosphokinase YwaC

EC=2.7.6.5
Alternative name(s):
(p)ppGpp synthase YwaC
Small alarmone synthase 2
Short name=SAS 2
Gene names
Name:ywaC
Ordered Locus Names:BSU38480
ORF Names:ipa-7d
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. activities in response to changes in nutritional abundance. YwaC has probably a minor role in stringent response. Ref.3

Catalytic activity

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Pathway

Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.

Induction

Expressed during exponential growth with a marked and transient increase at the onset of the stationary phase. Induced by alkaline shock. Ref.3

Miscellaneous

GDP can be used instead of GTP, resulting in an increase of (p)ppGpp synthesis. The synthase activity of YwaC is weaker than that of YjbM.

Sequence similarities

Belongs to the RelA/SpoT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210GTP pyrophosphokinase YwaC
PRO_0000049949

Sequences

Sequence LengthMass (Da)Tools
P39583 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 6F981DF0D31A14FC

FASTA21024,600
        10         20         30         40         50         60 
MDLSVTHMDD LKTVMEDWKN ELLVYKFALD ALDTKFSIIS QEYNLIHGHN PIEHTKSRVK 

        70         80         90        100        110        120 
SFESIVNKLM RKGCEITTKE MKEHIHDIAG VRIICSFISD IYNVVNVLKQ HEDLRIVKVK 

       130        140        150        160        170        180 
DYIQTPKPNG YRSLHLIIEM PVNLTNRVEY VKAEIQIRTI AMDFWASLEH KIYYKLNNDV 

       190        200        210 
PKQLTDELKE AAEIAHYLDE KMLGIKKEVD 

« Hide

References

« Hide 'large scale' references
[1]"Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325 degrees to 333 degrees."
Glaser P., Kunst F., Arnaud M., Coudart M.P., Gonzales W., Hullo M.-F., Ionescu M., Lubochinsky B., Marcelino L., Moszer I., Presecan E., Santana M., Schneider E., Schweizer J., Vertes A., Rapoport G., Danchin A.
Mol. Microbiol. 10:371-384(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis."
Nanamiya H., Kasai K., Nozawa A., Yun C.S., Narisawa T., Murakami K., Natori Y., Kawamura F., Tozawa Y.
Mol. Microbiol. 67:291-304(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A (P)PPGPP SYNTHASE, INDUCTION.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X73124 Genomic DNA. Translation: CAA51563.1.
AL009126 Genomic DNA. Translation: CAB15874.1.
PIRS39662.
RefSeqNP_391727.1. NC_000964.3.

3D structure databases

ProteinModelPortalP39583.
SMRP39583. Positions 16-210.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP39583. 1 interaction.
STRING224308.BSU38480.

Proteomic databases

PaxDbP39583.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15874; CAB15874; BSU38480.
GeneID937355.
KEGGbsu:BSU38480.
PATRIC18979742. VBIBacSub10457_4033.

Organism-specific databases

GenoListBSU38480.

Phylogenomic databases

eggNOGCOG2357.
HOGENOMHOG000263234.
OMAKIYYKLN.
OrthoDBEOG6P5ZJ6.
ProtClustDBCLSK2502636.

Enzyme and pathway databases

BioCycBSUB:BSU38480-MONOMER.
UniPathwayUPA00908; UER00884.

Family and domain databases

InterProIPR007685. RelA_SpoT.
[Graphical view]
PfamPF04607. RelA_SpoT. 1 hit.
[Graphical view]
SMARTSM00954. RelA_SpoT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameYWAC_BACSU
AccessionPrimary (citable) accession number: P39583
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 13, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList