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Protein

GTP pyrophosphokinase YwaC

Gene

ywaC

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. activities in response to changes in nutritional abundance. YwaC has probably a minor role in stringent response.1 Publication

Catalytic activityi

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. GTP binding Source: UniProtKB-KW
  3. GTP diphosphokinase activity Source: UniProtKB-EC
  4. kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU38480-MONOMER.
UniPathwayiUPA00908; UER00884.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP pyrophosphokinase YwaC (EC:2.7.6.5)
Alternative name(s):
(p)ppGpp synthase YwaC
Small alarmone synthase 2
Short name:
SAS 2
Gene namesi
Name:ywaC
Ordered Locus Names:BSU38480
ORF Names:ipa-7d
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU38480.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210GTP pyrophosphokinase YwaCPRO_0000049949Add
BLAST

Proteomic databases

PaxDbiP39583.

Expressioni

Inductioni

Expressed during exponential growth with a marked and transient increase at the onset of the stationary phase. Induced by alkaline shock.1 Publication

Interactioni

Protein-protein interaction databases

IntActiP39583. 1 interaction.
STRINGi224308.BSU38480.

Structurei

3D structure databases

SMRiP39583. Positions 16-210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated

Phylogenomic databases

eggNOGiCOG2357.
HOGENOMiHOG000263234.
InParanoidiP39583.
OMAiHINDIAG.
OrthoDBiEOG6P5ZJ6.
PhylomeDBiP39583.

Family and domain databases

InterProiIPR007685. RelA_SpoT.
[Graphical view]
PfamiPF04607. RelA_SpoT. 1 hit.
[Graphical view]
SMARTiSM00954. RelA_SpoT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLSVTHMDD LKTVMEDWKN ELLVYKFALD ALDTKFSIIS QEYNLIHGHN
60 70 80 90 100
PIEHTKSRVK SFESIVNKLM RKGCEITTKE MKEHIHDIAG VRIICSFISD
110 120 130 140 150
IYNVVNVLKQ HEDLRIVKVK DYIQTPKPNG YRSLHLIIEM PVNLTNRVEY
160 170 180 190 200
VKAEIQIRTI AMDFWASLEH KIYYKLNNDV PKQLTDELKE AAEIAHYLDE
210
KMLGIKKEVD
Length:210
Mass (Da):24,600
Last modified:February 1, 1995 - v1
Checksum:i6F981DF0D31A14FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73124 Genomic DNA. Translation: CAA51563.1.
AL009126 Genomic DNA. Translation: CAB15874.1.
PIRiS39662.
RefSeqiNP_391727.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15874; CAB15874; BSU38480.
GeneIDi937355.
KEGGibsu:BSU38480.
PATRICi18979742. VBIBacSub10457_4033.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73124 Genomic DNA. Translation: CAA51563.1.
AL009126 Genomic DNA. Translation: CAB15874.1.
PIRiS39662.
RefSeqiNP_391727.1. NC_000964.3.

3D structure databases

SMRiP39583. Positions 16-210.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP39583. 1 interaction.
STRINGi224308.BSU38480.

Proteomic databases

PaxDbiP39583.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15874; CAB15874; BSU38480.
GeneIDi937355.
KEGGibsu:BSU38480.
PATRICi18979742. VBIBacSub10457_4033.

Organism-specific databases

GenoListiBSU38480.

Phylogenomic databases

eggNOGiCOG2357.
HOGENOMiHOG000263234.
InParanoidiP39583.
OMAiHINDIAG.
OrthoDBiEOG6P5ZJ6.
PhylomeDBiP39583.

Enzyme and pathway databases

UniPathwayiUPA00908; UER00884.
BioCyciBSUB:BSU38480-MONOMER.

Family and domain databases

InterProiIPR007685. RelA_SpoT.
[Graphical view]
PfamiPF04607. RelA_SpoT. 1 hit.
[Graphical view]
SMARTiSM00954. RelA_SpoT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis."
    Nanamiya H., Kasai K., Nozawa A., Yun C.S., Narisawa T., Murakami K., Natori Y., Kawamura F., Tozawa Y.
    Mol. Microbiol. 67:291-304(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A (P)PPGPP SYNTHASE, INDUCTION.
    Strain: 168.

Entry informationi

Entry nameiYWAC_BACSU
AccessioniPrimary (citable) accession number: P39583
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 1, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

GDP can be used instead of GTP, resulting in an increase of (p)ppGpp synthesis. The synthase activity of YwaC is weaker than that of YjbM.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.