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Protein

Protein giant

Gene

gt

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Represses the expression of both the krueppel and knirps segmentation gap genes. Binds, in vitro, to the krueppel regulatory elements CD1 and CD2. It is required in the early embryo for the development of portions of the head and abdomen.

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase
  • RNA polymerase II distal enhancer sequence-specific DNA binding Source: FlyBase
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: FlyBase
  • sequence-specific DNA binding Source: FlyBase
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: GO_Central
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: FlyBase
  • transcription factor activity, sequence-specific DNA binding Source: FlyBase

GO - Biological processi

  • axon guidance Source: FlyBase
  • negative regulation of multicellular organism growth Source: FlyBase
  • negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • phagocytosis Source: FlyBase
  • posterior head segmentation Source: FlyBase
  • regulation of cell size Source: FlyBase
  • regulation of gene expression Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • regulation of transcription involved in anterior/posterior axis specification Source: FlyBase
  • ring gland development Source: FlyBase
  • salivary gland development Source: FlyBase
  • specification of segmental identity, labial segment Source: FlyBase
  • terminal region determination Source: FlyBase
  • torso signaling pathway Source: FlyBase
  • zygotic determination of anterior/posterior axis, embryo Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

SignaLinkiP39572.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein giant
Gene namesi
Name:gt
ORF Names:CG7952
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0001150. gt.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000766371 – 448Protein giantAdd BLAST448

Post-translational modificationi

Phosphorylated at multiple sites.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP39572.
PRIDEiP39572.

Expressioni

Gene expression databases

BgeeiFBgn0001150.
GenevisibleiP39572. DM.

Interactioni

Subunit structurei

Homodimer or heterodimer.Curated

GO - Molecular functioni

  • protein heterodimerization activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi57763. 21 interactors.
DIPiDIP-19651N.
IntActiP39572. 2 interactors.
MINTiMINT-286974.
STRINGi7227.FBpp0070444.

Structurei

3D structure databases

ProteinModelPortaliP39572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini384 – 447bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni390 – 406Basic motifPROSITE-ProRule annotationAdd BLAST17
Regioni407 – 414Leucine-zipperPROSITE-ProRule annotation8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi24 – 41His-richAdd BLAST18
Compositional biasi74 – 91Poly-GlnAdd BLAST18
Compositional biasi144 – 160Poly-GlnAdd BLAST17
Compositional biasi198 – 210Poly-AlaAdd BLAST13

Sequence similaritiesi

Belongs to the bZIP family.Curated

Phylogenomic databases

eggNOGiKOG3119. Eukaryota.
ENOG410YJ8G. LUCA.
GeneTreeiENSGT00440000039070.
InParanoidiP39572.
KOiK09062.
OMAiPRVERYT.
OrthoDBiEOG091G0IW2.
PhylomeDBiP39572.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiView protein in InterPro
IPR004827. bZIP.
IPR029859. Giant.
IPR015943. WD40/YVTN_repeat-like_dom_sf.
PANTHERiPTHR11988:SF25. PTHR11988:SF25. 4 hits.
PfamiView protein in Pfam
PF07716. bZIP_2. 1 hit.
SMARTiView protein in SMART
SM00338. BRLZ. 1 hit.
PROSITEiView protein in PROSITE
PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.

Sequencei

Sequence statusi: Complete.

P39572-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLMHEKLMAG QFFDLKTDRK PLMHHHQYQH HQQQPLHHLP HSQLPVQGSL
60 70 80 90 100
GLPKMDLYTA YAYQQQLLGA ALSQQQQQQQ QQQQHQQLQQ QHTSSAEVLD
110 120 130 140 150
LSRRCDSVET PRKTPSPYQT SYSYGSGSPS ASPTSNLLYA AQMQQQQHQQ
160 170 180 190 200
QQQQQQQQQQ LASLYPAFYY SNIKQEQATP TAAPPKVTPT ANLLQTFAAA
210 220 230 240 250
SAAAAAAAAA SSTNSPRPAS NASTMQIDVL ENPLSPAVEA TTPTTSSSGE
260 270 280 290 300
AGKNTRPFKA FPRDPLVIAA NFAATDVLLD NPRVERYTEY RKRVLEQIRS
310 320 330 340 350
SNGGSRTVTN PKMRRTNSRS GSVNEGSSSN NNSESEDRAA AEESSDCDSQ
360 370 380 390 400
AGNFESKSAT SSSSNLANAT AANSGISSGS QVKDAAYYER RRKNNAAAKK
410 420 430 440
SRDRRRIKED EIAIRAAYLE RQNIELLCQI DALKVQLAAF TSAKVTTA
Length:448
Mass (Da):49,169
Last modified:November 2, 2001 - v2
Checksum:i98EB75D082129439
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti234L → Q in CAA43456 (PubMed:1576969).Curated1
Sequence conflicti383K → R in AAQ22473 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61148 Genomic DNA. Translation: CAA43456.1.
AE014298 Genomic DNA. Translation: AAF45780.1.
AL133504 Genomic DNA. Translation: CAB63520.1.
BT010004 mRNA. Translation: AAQ22473.1.
BT057998 mRNA. Translation: ACM16708.1.
PIRiS17370.
RefSeqiNP_525049.1. NM_080310.3.
UniGeneiDm.5740.

Genome annotation databases

EnsemblMetazoaiFBtr0070460; FBpp0070444; FBgn0001150.
GeneIDi31227.
KEGGidme:Dmel_CG7952.
UCSCiCG7952-RB. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiGIANT_DROME
AccessioniPrimary (citable) accession number: P39572
Secondary accession number(s): B9EQS3, Q7YU39, Q9V3N3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 2, 2001
Last modified: November 22, 2017
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Caution

It is uncertain whether Met-1, Met-3, Met-8 or Met-23 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families