Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P39552

- MST28_YEAST

UniProt

P39552 - MST28_YEAST

Protein

Multicopy suppressor of SEC21 protein 28

Gene

MST28

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in protein trafficking vesicle formation, probably by stabilizing of coatomer at the Golgi membrane and thus allowing the efficient formation of COPI coated vesicles.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW
    2. vesicle-mediated transport Source: UniProtKB-KW
    3. vesicle organization Source: SGD

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28882-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Multicopy suppressor of SEC21 protein 28
    Alternative name(s):
    DUP240 protein MST28
    Gene namesi
    Name:MST28
    Ordered Locus Names:YAR033W
    ORF Names:FUN59
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAR033w.
    SGDiS000000079. MST28.

    Subcellular locationi

    GO - Cellular componenti

    1. COPI-coated vesicle membrane Source: UniProtKB-SubCell
    2. endoplasmic reticulum Source: SGD
    3. ER to Golgi transport vesicle membrane Source: UniProtKB-SubCell
    4. Golgi apparatus Source: SGD
    5. integral component of membrane Source: SGD
    6. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 234234Multicopy suppressor of SEC21 protein 28PRO_0000207526Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei3 – 31Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39552.

    Expressioni

    Gene expression databases

    GenevestigatoriP39552.

    Interactioni

    Subunit structurei

    Interacts with MST27. Binds to coatomer proteins of COPI and SEC23/SEC24 of COPII coated vesicles.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MST27P531763EBI-20785,EBI-6205

    Protein-protein interaction databases

    BioGridi31810. 17 interactions.
    DIPiDIP-1853N.
    IntActiP39552. 6 interactions.
    MINTiMINT-387935.
    STRINGi4932.YAR033W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39552.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 4747CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini69 – 724ExtracellularSequence Analysis
    Topological domaini94 – 234141CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei48 – 6821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei73 – 9321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni231 – 2344COPI binding

    Sequence similaritiesi

    Belongs to the DUP/COS family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00390000017646.
    HOGENOMiHOG000000896.
    OrthoDBiEOG72JWSS.

    Family and domain databases

    InterProiIPR001142. DUP.
    [Graphical view]
    PfamiPF00674. DUP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39552-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQTPPESTDV KLDTLNEPSA HLIEKNVALP KDIFRSYLSY WIYEIARYTP    50
    VMILSLVIGV LVLLIIFFND NEACVFNSAI FAFTSLVGLL IILSDGNPKL 100
    VSRRNFRTEL LVDVITRKPA VEGKEWRIIT YNMNQYLFNH GQWHTPYYFY 150
    SDEDCYRYFL RLVEGVTPKK QTATSIGNSP VTAKPEDAIE SASPSSRLNY 200
    QNFLLKAAEI ERQAQENYWR RRHPNIDALL KKTE 234
    Length:234
    Mass (Da):27,091
    Last modified:February 1, 1995 - v1
    Checksum:iB23AAD4D26831319
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L28920 Genomic DNA. Translation: AAC09494.1.
    BK006935 Genomic DNA. Translation: DAA07003.1.
    PIRiS53484.
    RefSeqiNP_009419.1. NM_001178225.1.

    Genome annotation databases

    EnsemblFungiiYAR033W; YAR033W; YAR033W.
    GeneIDi851284.
    KEGGisce:YAR033W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L28920 Genomic DNA. Translation: AAC09494.1 .
    BK006935 Genomic DNA. Translation: DAA07003.1 .
    PIRi S53484.
    RefSeqi NP_009419.1. NM_001178225.1.

    3D structure databases

    ProteinModelPortali P39552.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31810. 17 interactions.
    DIPi DIP-1853N.
    IntActi P39552. 6 interactions.
    MINTi MINT-387935.
    STRINGi 4932.YAR033W.

    Proteomic databases

    MaxQBi P39552.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAR033W ; YAR033W ; YAR033W .
    GeneIDi 851284.
    KEGGi sce:YAR033W.

    Organism-specific databases

    CYGDi YAR033w.
    SGDi S000000079. MST28.

    Phylogenomic databases

    GeneTreei ENSGT00390000017646.
    HOGENOMi HOG000000896.
    OrthoDBi EOG72JWSS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28882-MONOMER.

    Miscellaneous databases

    NextBioi 968282.

    Gene expression databases

    Genevestigatori P39552.

    Family and domain databases

    InterProi IPR001142. DUP.
    [Graphical view ]
    Pfami PF00674. DUP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 52 Kbp CDC15-FLO1-PHO11-YAR074 region."
      Bussey H., Keng T., Storms R.K., Vo D., Zhong W., Fortin N., Barton A.B., Kaback D.B., Clark M.W.
      Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability."
      Poirey R., Despons L., Leh V., Lafuente M.-J., Potier S., Souciet J.-L., Jauniaux J.-C.
      Microbiology 148:2111-2123(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    5. "Suppression of coatomer mutants by a new protein family with COPI and COPII binding motifs in Saccharomyces cerevisiae."
      Sandmann T., Herrmann J.M., Dengjel J., Schwarz H., Spang A.
      Mol. Biol. Cell 14:3097-3113(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MST27.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-3, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMST28_YEAST
    AccessioniPrimary (citable) accession number: P39552
    Secondary accession number(s): D6VPN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Members of the DUP240 multigene family are specific to S.cerevisiae sensu strictu. Cells lacking all 10 DUP240 proteins show no obvious alterations in mating, sporulation and cell growth.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3