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P39552 (MST28_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multicopy suppressor of SEC21 protein 28
Alternative name(s):
DUP240 protein MST28
Gene names
Name:MST28
Ordered Locus Names:YAR033W
ORF Names:FUN59
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length234 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in protein trafficking vesicle formation, probably by stabilizing of coatomer at the Golgi membrane and thus allowing the efficient formation of COPI coated vesicles. Ref.5

Subunit structure

Interacts with MST27. Binds to coatomer proteins of COPI and SEC23/SEC24 of COPII coated vesicles. Ref.5

Subcellular location

Endoplasmic reticulum. Golgi apparatus. Cytoplasmic vesicleCOPI-coated vesicle membrane; Multi-pass membrane protein Potential. Cytoplasmic vesicleCOPII-coated vesicle membrane; Multi-pass membrane protein Potential Ref.4 Ref.5.

Miscellaneous

Members of the DUP240 multigene family are specific to S.cerevisiae sensu strictu. Cells lacking all 10 DUP240 proteins show no obvious alterations in mating, sporulation and cell growth.

Sequence similarities

Belongs to the DUP/COS family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MST27P531763EBI-20785,EBI-6205

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 234234Multicopy suppressor of SEC21 protein 28
PRO_0000207526

Regions

Topological domain1 – 4747Cytoplasmic Potential
Transmembrane48 – 6821Helical; Potential
Topological domain69 – 724Extracellular Potential
Transmembrane73 – 9321Helical; Potential
Topological domain94 – 234141Cytoplasmic Potential
Region231 – 2344COPI binding

Amino acid modifications

Modified residue31Phosphothreonine Ref.6

Sequences

Sequence LengthMass (Da)Tools
P39552 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: B23AAD4D26831319

FASTA23427,091
        10         20         30         40         50         60 
MQTPPESTDV KLDTLNEPSA HLIEKNVALP KDIFRSYLSY WIYEIARYTP VMILSLVIGV 

        70         80         90        100        110        120 
LVLLIIFFND NEACVFNSAI FAFTSLVGLL IILSDGNPKL VSRRNFRTEL LVDVITRKPA 

       130        140        150        160        170        180 
VEGKEWRIIT YNMNQYLFNH GQWHTPYYFY SDEDCYRYFL RLVEGVTPKK QTATSIGNSP 

       190        200        210        220        230 
VTAKPEDAIE SASPSSRLNY QNFLLKAAEI ERQAQENYWR RRHPNIDALL KKTE 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 52 Kbp CDC15-FLO1-PHO11-YAR074 region."
Bussey H., Keng T., Storms R.K., Vo D., Zhong W., Fortin N., Barton A.B., Kaback D.B., Clark M.W.
Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability."
Poirey R., Despons L., Leh V., Lafuente M.-J., Potier S., Souciet J.-L., Jauniaux J.-C.
Microbiology 148:2111-2123(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[5]"Suppression of coatomer mutants by a new protein family with COPI and COPII binding motifs in Saccharomyces cerevisiae."
Sandmann T., Herrmann J.M., Dengjel J., Schwarz H., Spang A.
Mol. Biol. Cell 14:3097-3113(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MST27.
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-3, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L28920 Genomic DNA. Translation: AAC09494.1.
BK006935 Genomic DNA. Translation: DAA07003.1.
PIRS53484.
RefSeqNP_009419.1. NM_001178225.1.

3D structure databases

ProteinModelPortalP39552.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31810. 17 interactions.
DIPDIP-1853N.
IntActP39552. 6 interactions.
MINTMINT-387935.
STRING4932.YAR033W.

Proteomic databases

MaxQBP39552.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAR033W; YAR033W; YAR033W.
GeneID851284.
KEGGsce:YAR033W.

Organism-specific databases

CYGDYAR033w.
SGDS000000079. MST28.

Phylogenomic databases

GeneTreeENSGT00390000017646.
HOGENOMHOG000000896.
OrthoDBEOG72JWSS.

Enzyme and pathway databases

BioCycYEAST:G3O-28882-MONOMER.

Gene expression databases

GenevestigatorP39552.

Family and domain databases

InterProIPR001142. DUP.
[Graphical view]
PfamPF00674. DUP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968282.

Entry information

Entry nameMST28_YEAST
AccessionPrimary (citable) accession number: P39552
Secondary accession number(s): D6VPN3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families