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Protein

Multicopy suppressor of SEC21 protein 28

Gene

MST28

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in protein trafficking vesicle formation, probably by stabilizing of coatomer at the Golgi membrane and thus allowing the efficient formation of COPI coated vesicles.1 Publication

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
  2. vesicle-mediated transport Source: UniProtKB-KW
  3. vesicle organization Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-28882-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Multicopy suppressor of SEC21 protein 28
Alternative name(s):
DUP240 protein MST28
Gene namesi
Name:MST28
Ordered Locus Names:YAR033W
ORF Names:FUN59
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAR033w.
SGDiS000000079. MST28.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4747CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei48 – 6821HelicalSequence AnalysisAdd
BLAST
Topological domaini69 – 724ExtracellularSequence Analysis
Transmembranei73 – 9321HelicalSequence AnalysisAdd
BLAST
Topological domaini94 – 234141CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. COPI-coated vesicle membrane Source: UniProtKB-SubCell
  2. endoplasmic reticulum Source: SGD
  3. ER to Golgi transport vesicle membrane Source: UniProtKB-SubCell
  4. Golgi apparatus Source: SGD
  5. integral component of membrane Source: SGD
  6. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 234234Multicopy suppressor of SEC21 protein 28PRO_0000207526Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei3 – 31Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39552.

Expressioni

Gene expression databases

GenevestigatoriP39552.

Interactioni

Subunit structurei

Interacts with MST27. Binds to coatomer proteins of COPI and SEC23/SEC24 of COPII coated vesicles.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MST27P531763EBI-20785,EBI-6205

Protein-protein interaction databases

BioGridi31810. 17 interactions.
DIPiDIP-1853N.
IntActiP39552. 6 interactions.
MINTiMINT-387935.
STRINGi4932.YAR033W.

Structurei

3D structure databases

ProteinModelPortaliP39552.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni231 – 2344COPI binding

Sequence similaritiesi

Belongs to the DUP/COS family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000017646.
HOGENOMiHOG000000896.
InParanoidiP39552.
OrthoDBiEOG72JWSS.

Family and domain databases

InterProiIPR001142. DUP.
[Graphical view]
PfamiPF00674. DUP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39552-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQTPPESTDV KLDTLNEPSA HLIEKNVALP KDIFRSYLSY WIYEIARYTP
60 70 80 90 100
VMILSLVIGV LVLLIIFFND NEACVFNSAI FAFTSLVGLL IILSDGNPKL
110 120 130 140 150
VSRRNFRTEL LVDVITRKPA VEGKEWRIIT YNMNQYLFNH GQWHTPYYFY
160 170 180 190 200
SDEDCYRYFL RLVEGVTPKK QTATSIGNSP VTAKPEDAIE SASPSSRLNY
210 220 230
QNFLLKAAEI ERQAQENYWR RRHPNIDALL KKTE
Length:234
Mass (Da):27,091
Last modified:February 1, 1995 - v1
Checksum:iB23AAD4D26831319
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28920 Genomic DNA. Translation: AAC09494.1.
BK006935 Genomic DNA. Translation: DAA07003.1.
PIRiS53484.
RefSeqiNP_009419.1. NM_001178225.1.

Genome annotation databases

EnsemblFungiiYAR033W; YAR033W; YAR033W.
GeneIDi851284.
KEGGisce:YAR033W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L28920 Genomic DNA. Translation: AAC09494.1.
BK006935 Genomic DNA. Translation: DAA07003.1.
PIRiS53484.
RefSeqiNP_009419.1. NM_001178225.1.

3D structure databases

ProteinModelPortaliP39552.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31810. 17 interactions.
DIPiDIP-1853N.
IntActiP39552. 6 interactions.
MINTiMINT-387935.
STRINGi4932.YAR033W.

Proteomic databases

MaxQBiP39552.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAR033W; YAR033W; YAR033W.
GeneIDi851284.
KEGGisce:YAR033W.

Organism-specific databases

CYGDiYAR033w.
SGDiS000000079. MST28.

Phylogenomic databases

GeneTreeiENSGT00390000017646.
HOGENOMiHOG000000896.
InParanoidiP39552.
OrthoDBiEOG72JWSS.

Enzyme and pathway databases

BioCyciYEAST:G3O-28882-MONOMER.

Miscellaneous databases

NextBioi968282.

Gene expression databases

GenevestigatoriP39552.

Family and domain databases

InterProiIPR001142. DUP.
[Graphical view]
PfamiPF00674. DUP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 52 Kbp CDC15-FLO1-PHO11-YAR074 region."
    Bussey H., Keng T., Storms R.K., Vo D., Zhong W., Fortin N., Barton A.B., Kaback D.B., Clark M.W.
    Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability."
    Poirey R., Despons L., Leh V., Lafuente M.-J., Potier S., Souciet J.-L., Jauniaux J.-C.
    Microbiology 148:2111-2123(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  5. "Suppression of coatomer mutants by a new protein family with COPI and COPII binding motifs in Saccharomyces cerevisiae."
    Sandmann T., Herrmann J.M., Dengjel J., Schwarz H., Spang A.
    Mol. Biol. Cell 14:3097-3113(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MST27.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-3, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMST28_YEAST
AccessioniPrimary (citable) accession number: P39552
Secondary accession number(s): D6VPN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 7, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Members of the DUP240 multigene family are specific to S.cerevisiae sensu strictu. Cells lacking all 10 DUP240 proteins show no obvious alterations in mating, sporulation and cell growth.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.