Multicopy suppressor of SEC21 protein 28 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P39552 (MST28_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Multicopy suppressor of SEC21 protein 28

Alternative name(s):
DUP240 protein MST28

Gene names

Name:	MST28
Ordered Locus Names:	YAR033W
ORF Names:	FUN59

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in protein trafficking vesicle formation, probably by stabilizing of coatomer at the Golgi membrane and thus allowing the efficient formation of COPI coated vesicles. Ref.5
Subunit structure	Interacts with MST27. Binds to coatomer proteins of COPI and SEC23/SEC24 of COPII coated vesicles. Ref.5
Subcellular location	Endoplasmic reticulum. Golgi apparatus. Cytoplasmic vesicle › COPI-coated vesicle membrane; Multi-pass membrane protein Potential. Cytoplasmic vesicle › COPII-coated vesicle membrane; Multi-pass membrane protein Potential Ref.4 Ref.5.
Miscellaneous	Members of the DUP240 multigene family are specific to S.cerevisiae sensu strictu. Cells lacking all 10 DUP240 proteins show no obvious alterations in mating, sporulation and cell growth.
Sequence similarities	Belongs to the DUP/COS family.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasmic vesicle Endoplasmic reticulum Golgi apparatus Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle organization Inferred from physical interaction Ref.5. Source: SGD vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	COPI-coated vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell ER to Golgi transport vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi apparatus Inferred from physical interaction Ref.5. Source: SGD endoplasmic reticulum Inferred from mutant phenotype Ref.4. Source: SGD integral to membrane Inferred from direct assay Ref.5. Source: SGD plasma membrane Inferred from mutant phenotype Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-20785,EBI-20785
MST27	P53176	2	EBI-20785,EBI-6205

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 234

234

Multicopy suppressor of SEC21 protein 28

PRO_0000207526

Regions

Topological domain

1 – 47

Cytoplasmic Potential

Transmembrane

48 – 68

Helical; Potential

Topological domain

69 – 72

Extracellular Potential

Transmembrane

73 – 93

Helical; Potential

Topological domain

94 – 234

141

Cytoplasmic Potential

Region

231 – 234

COPI binding

Amino acid modifications

Modified residue

Phosphothreonine Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

P39552 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: B23AAD4D26831319
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FASTA

234

27,091

        10         20         30         40         50         60 
MQTPPESTDV KLDTLNEPSA HLIEKNVALP KDIFRSYLSY WIYEIARYTP VMILSLVIGV 

        70         80         90        100        110        120 
LVLLIIFFND NEACVFNSAI FAFTSLVGLL IILSDGNPKL VSRRNFRTEL LVDVITRKPA 

       130        140        150        160        170        180 
VEGKEWRIIT YNMNQYLFNH GQWHTPYYFY SDEDCYRYFL RLVEGVTPKK QTATSIGNSP 

       190        200        210        220        230 
VTAKPEDAIE SASPSSRLNY QNFLLKAAEI ERQAQENYWR RRHPNIDALL KKTE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 52 Kbp CDC15-FLO1-PHO11-YAR074 region." Bussey H., Keng T., Storms R.K., Vo D., Zhong W., Fortin N., Barton A.B., Kaback D.B., Clark M.W. Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability." Poirey R., Despons L., Leh V., Lafuente M.-J., Potier S., Souciet J.-L., Jauniaux J.-C. Microbiology 148:2111-2123(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"Suppression of coatomer mutants by a new protein family with COPI and COPII binding motifs in Saccharomyces cerevisiae." Sandmann T., Herrmann J.M., Dengjel J., Schwarz H., Spang A. Mol. Biol. Cell 14:3097-3113(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MST27.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-3, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L28920 Genomic DNA. Translation: AAC09494.1. BK006935 Genomic DNA. Translation: DAA07003.1.
PIR	S53484.
RefSeq	NP_009419.1. NM_001178225.1.
3D structure databases
ProteinModelPortal	P39552.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1853N.
IntAct	P39552. 4 interactions.
MINT	MINT-387935.
STRING	4932.YAR033W.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAR033W; YAR033W; YAR033W.
GeneID	851284.
KEGG	sce:YAR033W.
Organism-specific databases
CYGD	YAR033w.
SGD	S000000079. MST28.
Phylogenomic databases
GeneTree	ENSGT00390000017646.
HOGENOM	HOG000000896.
Gene expression databases
ArrayExpress	P39552.
Genevestigator	P39552.
GermOnline	YAR033W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001142. DUP. [Graphical view]
Pfam	PF00674. DUP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968282.

Entry information

Entry name

MST28_YEAST

Accession

Primary (citable) accession number: P39552
Secondary accession number(s): D6VPN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents