P39547 (UIP3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ULP1-interacting protein 3

Alternative name(s):
DUP240 protein UIP3

Gene names

Name:	UIP3
Ordered Locus Names:	YAR027W
ORF Names:	FUN55

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Interacts with ULP1. Ref.4
Subcellular location	Nucleus membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein Ref.4.
Miscellaneous	Present with 1695 molecules/cell in log phase SD medium. Members of the DUP240 multigene family are specific to S.cerevisiae sensu strictu. Cells lacking all 10 DUP240 proteins show no obvious alterations in mating, sporulation and cell growth.
Sequence similarities	Belongs to the DUP/COS family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Nucleus
Domain	Transmembrane Transmembrane helix
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nuclear envelope Inferred from direct assay Ref.4. Source: SGD nuclear membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	ULP1-interacting protein 3		PRO_0000207528
Regions
	Transmembrane	51 – 71	21	Helical; Potential
	Transmembrane	73 – 93	21	Helical; Potential
Amino acid modifications
	Cross-link	11		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7
	Cross-link	218		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P39547 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 9DBF29F9C2395AB2	FASTA	235	27,117
	10 20 30 40 50 60 MQTPSENTDV KLDTLDEPSA HLIEENVALP EDTFNSYWSY ILNEIARCKP LMIMFLIPVC 70 80 90 100 110 120 LVLLITFFHD IKGILVFLVI SLILSIIILL IGITAFVSET LNKGFIIKLL VEVITRKPAV 130 140 150 160 170 180 GGKEWRIIAY NMNQYLFDHG IWHTPYYFFC EHRCHKFFKS LIKQTRSNAH LSSPTNGAEN 190 200 210 220 230 TQSNTPAKEV SNEMVKPYIF SSDPVLEAYL IKAAEIHKEA EFEYWRKQYP EVDLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 52 Kbp CDC15-FLO1-PHO11-YAR074 region." Bussey H., Keng T., Storms R.K., Vo D., Zhong W., Fortin N., Barton A.B., Kaback D.B., Clark M.W. Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Yeast Ulp1, an Smt3-specific protease, associates with nucleoporins." Takahashi Y., Mizoi J., Toh-e A., Kikuchi Y. J. Biochem. 128:723-725(2000) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH ULP1.
[5]	"Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability." Poirey R., Despons L., Leh V., Lafuente M.-J., Potier S., Souciet J.-L., Jauniaux J.-C. Microbiology 148:2111-2123(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NULL MUTANT.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-11 AND LYS-218, MASS SPECTROMETRY. Strain: SUB592.
[8]	"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution." Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O. Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]	"Sites of ubiquitin attachment in Saccharomyces cerevisiae." Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S. Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L28920 Genomic DNA. Translation: AAC09489.1. BK006935 Genomic DNA. Translation: DAA06999.1.
PIR	S53479.
RefSeq	NP_009414.1. NM_001178221.1.
3D structure databases
ProteinModelPortal	P39547.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2031N.
IntAct	P39547. 52 interactions.
MINT	MINT-516292.
STRING	4932.YAR027W.
Proteomic databases
PaxDb	P39547.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAR027W; YAR027W; YAR027W.
GeneID	851279.
KEGG	sce:YAR027W.
Organism-specific databases
CYGD	YAR027w.
SGD	S000000075. UIP3.
Phylogenomic databases
GeneTree	ENSGT00390000017646.
HOGENOM	HOG000000896.
OrthoDB	EOG72JWSS.
Enzyme and pathway databases
BioCyc	YEAST:G3O-28878-MONOMER.
Gene expression databases
ArrayExpress	P39547.
Genevestigator	P39547.
Family and domain databases
InterPro	IPR001142. DUP. [Graphical view]
Pfam	PF00674. DUP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968270.

Entry information

Entry name

UIP3_YEAST

Accession

Primary (citable) accession number: P39547
Secondary accession number(s): D6VPM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 13, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program