Ubiquitin carboxyl-terminal hydrolase 12 - Saccharomyces cerevisiae (Baker's yeast)

Names and origin

Protein names

Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 12
    EC=3.1.2.15
Alternative name(s):
    Ubiquitin thioesterase 12
    Ubiquitin-specific-processing protease 12
    Deubiquitinating enzyme 12

Gene names

Name:	UBP12
Ordered Locus Names:	YJL197W
ORF Names:	J0340

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length	1254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity

Ubiquitin C-terminal thioester + H₂O = ubiquitin + a thiol.

Miscellaneous

Present with 7110 molecules/cell in log phase SD medium. Ref.3

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 DUSP domain.

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Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: EC ubiquitin-specific protease activity Traceable author statement. Source: SGD
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1254

1254

Ubiquitin carboxyl-terminal hydrolase 12

PRO_0000080597

Regions

Domain

97 – 199

103

DUSP

Sites

Active site

373

1

By similarity

Active site

1060

1

By similarity

Active site

1068

1

By similarity

Amino acid modifications

Modified residue

12

1

Phosphoserine Ref.4

Modified residue

84

1

Phosphoserine Ref.4

Modified residue

86

1

Phosphothreonine Ref.4

Modified residue

430

1

Phosphoserine Ref.4

Modified residue

1153

1

Phosphothreonine Ref.4

Modified residue

1226

1

Phosphoserine Ref.4

Modified residue

1227

1

Phosphothreonine Ref.4

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Sequences

Sequence

Length

Mass (Da)

Tools

P39538-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 349AFA4C4CEE0EA5
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FASTA

1,254

143,192

        10         20         30         40         50         60 
MGSSDVSSRE CSLVYNEDPD FTDGTTPCDR LGVDLMNVLD DKDEIKQESV PVSDREIEDT 

        70         80         90        100        110        120 
ESDASAVSSF ASANELIAEP HAASETNLGT NGQDGRNVLE QQRDVVARLI EENKETQKEG 

       130        140        150        160        170        180 
DKVCIVPKVW YDKFFDPDVT DPEDIGPINT RMICRDFENF VLEDYNRCPY LSIAEPVFNF 

       190        200        210        220        230        240 
LSEIYGMTSG SYPVVTNLVI NQTTGELETE YNKWFFRLHY LTEKQDGRKR RHGQDDSIMY 

       250        260        270        280        290        300 
LSMSALNLVR DLVEKSMNLF FEKADHLDVN AVDFKIWFVS EGSDIATDSN VSTFLNSSYE 

       310        320        330        340        350        360 
ITPLQFLELP IKKLLIPDMF ENRLDKITSN PSDLVIEIKP IEGNHHWPSN YFAYNKLEPA 

       370        380        390        400        410        420 
SGTTGLVNLG NTCYMNSALQ CLVHIPQLRD YFLYDGYEDE INEENPLGYH GYVARAFSDL 

       430        440        450        460        470        480 
VQKLFQNRMS IMQRNAAFPP SMFKSTIGHF NSMFSGYMQQ DSQEFLAFLL DSLHEDLNRI 

       490        500        510        520        530        540 
IKKEYTEKPS LSPGDDVNDW NVVKKLADDT WEMHLKRNCS VITDLFVGMY KSTLYCPECQ 

       550        560        570        580        590        600 
NVSITFDPYN DVTLPLPVDT VWDKTIKIFP MNSPPLLLEV ELSKSSTYMD LKNYVGKMSG 

       610        620        630        640        650        660 
LDPNTLFGCE IFSNQIYVNY ESTESNAQFL TLQELIKPAD DVIFYELPVT NDNEVIVPVL 

       670        680        690        700        710        720 
NTRIEKGYKN AMLFGVPFFI TLKEDELNNP GAIRMKLQNR FVHLSGGYIP FPEPVGNRTD 

       730        740        750        760        770        780 
FADAFPLLVE KYPDVEFEQY KDILQYTSIK VTDKDKSFFS IKILSVEKEQ QFASNNRTGP 

       790        800        810        820        830        840 
NFWTPISQLN LDKATDIDDK LEDVVKDIYN YSSLVDCAEG VLMQVDDEGD TEGSEAKNFS 

       850        860        870        880        890        900 
KPFQSGDDEE NKETVTNNEN VNNTNDRDED MELTDDVEED ASTEPELTDK PEALDKIKDS 

       910        920        930        940        950        960 
LTSTPFAILS MNDIIVCEWS ELGSNEAFSD DKIYNWENPA TLPNKELENA KLERSNAKER 

       970        980        990       1000       1010       1020 
TITLDDCLQL FSKPEILGLT DSWYCPTCKE HRQATKQIQL WNTPDILLIH LKRFESQRSF 

      1030       1040       1050       1060       1070       1080 
SDKIDATVNF PITDLDLSRY VVYKDDPRGL IYDLYAVDNH YGGLGGGHYT AYVKNFADNK 

      1090       1100       1110       1120       1130       1140 
WYYFDDSRVT ETAPENSIAG SAYLLFYIRR HKDGNGLGSS KLQEIIQKSR HGYDERIKKI 

      1150       1160       1170       1180       1190       1200 
YDEQMKLYEF NKTDEEEDVS DDMIECNEDV QAPEYSNRSL EVGHIETQDC NDEDDNDDGE 

      1210       1220       1230       1240       1250 
RTNSGRRKLR LLKKVYKNNS GLGSSSTSEI SEGCPENEVA DLNLKNGVTL ESPE

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes." Purnelle B., Coster F., Goffeau A. Yeast 10:1235-1249(1994) [PubMed: 7754713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[4]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; SER-84; THR-86; SER-430; THR-1153; SER-1226 AND THR-1227, MASS SPECTROMETRY.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X77688 Genomic DNA. Translation: CAA54762.1. Z49472 Genomic DNA. Translation: CAA89492.1.
PIR	S46636.
RefSeq	NP_012338.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6312N.
IntAct	P39538. 4 interactions.
STRING	P39538.
Proteomic databases
PeptideAtlas	P39538.
PRIDE	P39538.
Genome annotation databases
Ensembl	YJL197W; YJL197W; YJL197W; Saccharomyces cerevisiae. [Genome view]
GeneID	853242.
KEGG	sce:YJL197W.
NMPDR	fig\|4932.3.peg.3301.
Organism-specific databases
CYGD	YJL197w.
SGD	S000003733. UBP12.
Phylogenomic databases
HOGENOM	P39538.
OMA	WYHTFTI
OrthoDB	EOG9GQRNC
Enzyme and pathway databases
BRENDA	3.1.2.15. 250.
Gene expression databases
ArrayExpress	P39538.
Genevestigator	P39538.
GermOnline	YJL197W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006615. Pept_C19_DUSP. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
SMART	SM00695. DUSP. 1 hit. [Graphical view]
PROSITE	PS51283. DUSP. 1 hit. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973472.

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Entry information

Entry name

UBP12_YEAST

Accession

Primary (citable) accession number: P39538

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 24, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents