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P39538

- UBP12_YEAST

UniProt

P39538 - UBP12_YEAST

Protein

Ubiquitin carboxyl-terminal hydrolase 12

Gene

UBP12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Ubiquitin carboxyl-terminal hydrolase that recognizes ubiquitin chains that stabilize FZO1 and promote mitochondrial fusion. UBP12 deubiquitylates FZO1 only after oligomerization.1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei373 – 3731NucleophilePROSITE-ProRule annotation
    Active sitei1068 – 10681Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: SGD

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31628-MONOMER.

    Protein family/group databases

    MEROPSiC19.103.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 12 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 12
    Ubiquitin thioesterase 12
    Ubiquitin-specific-processing protease 12
    Gene namesi
    Name:UBP12
    Ordered Locus Names:YJL197W
    ORF Names:J0340
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL197w.
    SGDiS000003733. UBP12.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12541254Ubiquitin carboxyl-terminal hydrolase 12PRO_0000080597Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei84 – 841Phosphoserine1 Publication
    Modified residuei1160 – 11601Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39538.
    PaxDbiP39538.
    PeptideAtlasiP39538.
    PRIDEiP39538.

    Expressioni

    Gene expression databases

    GenevestigatoriP39538.

    Interactioni

    Subunit structurei

    Interacts with FZO1.1 Publication

    Protein-protein interaction databases

    BioGridi33567. 26 interactions.
    DIPiDIP-6312N.
    IntActiP39538. 6 interactions.
    MINTiMINT-704453.
    STRINGi4932.YJL197W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39538.
    SMRiP39538. Positions 362-561, 961-1110.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini97 – 199103DUSPPROSITE-ProRule annotationAdd
    BLAST
    Domaini364 – 1110747USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 DUSP domain.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5560.
    GeneTreeiENSGT00670000097750.
    HOGENOMiHOG000057142.
    KOiK11835.
    OMAiKWYHTFT.
    OrthoDBiEOG7R2BSX.

    Family and domain databases

    Gene3Di3.30.2230.10. 1 hit.
    InterProiIPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00695. DUSP. 1 hit.
    [Graphical view]
    SUPFAMiSSF143791. SSF143791. 1 hit.
    PROSITEiPS51283. DUSP. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39538-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSSDVSSRE CSLVYNEDPD FTDGTTPCDR LGVDLMNVLD DKDEIKQESV     50
    PVSDREIEDT ESDASAVSSF ASANELIAEP HAASETNLGT NGQDGRNVLE 100
    QQRDVVARLI EENKETQKEG DKVCIVPKVW YDKFFDPDVT DPEDIGPINT 150
    RMICRDFENF VLEDYNRCPY LSIAEPVFNF LSEIYGMTSG SYPVVTNLVI 200
    NQTTGELETE YNKWFFRLHY LTEKQDGRKR RHGQDDSIMY LSMSALNLVR 250
    DLVEKSMNLF FEKADHLDVN AVDFKIWFVS EGSDIATDSN VSTFLNSSYE 300
    ITPLQFLELP IKKLLIPDMF ENRLDKITSN PSDLVIEIKP IEGNHHWPSN 350
    YFAYNKLEPA SGTTGLVNLG NTCYMNSALQ CLVHIPQLRD YFLYDGYEDE 400
    INEENPLGYH GYVARAFSDL VQKLFQNRMS IMQRNAAFPP SMFKSTIGHF 450
    NSMFSGYMQQ DSQEFLAFLL DSLHEDLNRI IKKEYTEKPS LSPGDDVNDW 500
    NVVKKLADDT WEMHLKRNCS VITDLFVGMY KSTLYCPECQ NVSITFDPYN 550
    DVTLPLPVDT VWDKTIKIFP MNSPPLLLEV ELSKSSTYMD LKNYVGKMSG 600
    LDPNTLFGCE IFSNQIYVNY ESTESNAQFL TLQELIKPAD DVIFYELPVT 650
    NDNEVIVPVL NTRIEKGYKN AMLFGVPFFI TLKEDELNNP GAIRMKLQNR 700
    FVHLSGGYIP FPEPVGNRTD FADAFPLLVE KYPDVEFEQY KDILQYTSIK 750
    VTDKDKSFFS IKILSVEKEQ QFASNNRTGP NFWTPISQLN LDKATDIDDK 800
    LEDVVKDIYN YSSLVDCAEG VLMQVDDEGD TEGSEAKNFS KPFQSGDDEE 850
    NKETVTNNEN VNNTNDRDED MELTDDVEED ASTEPELTDK PEALDKIKDS 900
    LTSTPFAILS MNDIIVCEWS ELGSNEAFSD DKIYNWENPA TLPNKELENA 950
    KLERSNAKER TITLDDCLQL FSKPEILGLT DSWYCPTCKE HRQATKQIQL 1000
    WNTPDILLIH LKRFESQRSF SDKIDATVNF PITDLDLSRY VVYKDDPRGL 1050
    IYDLYAVDNH YGGLGGGHYT AYVKNFADNK WYYFDDSRVT ETAPENSIAG 1100
    SAYLLFYIRR HKDGNGLGSS KLQEIIQKSR HGYDERIKKI YDEQMKLYEF 1150
    NKTDEEEDVS DDMIECNEDV QAPEYSNRSL EVGHIETQDC NDEDDNDDGE 1200
    RTNSGRRKLR LLKKVYKNNS GLGSSSTSEI SEGCPENEVA DLNLKNGVTL 1250
    ESPE 1254
    Length:1,254
    Mass (Da):143,192
    Last modified:February 1, 1995 - v1
    Checksum:i349AFA4C4CEE0EA5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77688 Genomic DNA. Translation: CAA54762.1.
    Z49472 Genomic DNA. Translation: CAA89492.1.
    BK006943 Genomic DNA. Translation: DAA08611.1.
    PIRiS46636.
    RefSeqiNP_012338.1. NM_001181630.1.

    Genome annotation databases

    EnsemblFungiiYJL197W; YJL197W; YJL197W.
    GeneIDi853242.
    KEGGisce:YJL197W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77688 Genomic DNA. Translation: CAA54762.1 .
    Z49472 Genomic DNA. Translation: CAA89492.1 .
    BK006943 Genomic DNA. Translation: DAA08611.1 .
    PIRi S46636.
    RefSeqi NP_012338.1. NM_001181630.1.

    3D structure databases

    ProteinModelPortali P39538.
    SMRi P39538. Positions 362-561, 961-1110.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33567. 26 interactions.
    DIPi DIP-6312N.
    IntActi P39538. 6 interactions.
    MINTi MINT-704453.
    STRINGi 4932.YJL197W.

    Protein family/group databases

    MEROPSi C19.103.

    Proteomic databases

    MaxQBi P39538.
    PaxDbi P39538.
    PeptideAtlasi P39538.
    PRIDEi P39538.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL197W ; YJL197W ; YJL197W .
    GeneIDi 853242.
    KEGGi sce:YJL197W.

    Organism-specific databases

    CYGDi YJL197w.
    SGDi S000003733. UBP12.

    Phylogenomic databases

    eggNOGi COG5560.
    GeneTreei ENSGT00670000097750.
    HOGENOMi HOG000057142.
    KOi K11835.
    OMAi KWYHTFT.
    OrthoDBi EOG7R2BSX.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31628-MONOMER.

    Miscellaneous databases

    NextBioi 973472.

    Gene expression databases

    Genevestigatori P39538.

    Family and domain databases

    Gene3Di 3.30.2230.10. 1 hit.
    InterProi IPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00695. DUSP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF143791. SSF143791. 1 hit.
    PROSITEi PS51283. DUSP. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes."
      Purnelle B., Coster F., Goffeau A.
      Yeast 10:1235-1249(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-84, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1160, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Two deubiquitylases act on mitofusin and regulate mitochondrial fusion along independent pathways."
      Anton F., Dittmar G., Langer T., Escobar-Henriques M.
      Mol. Cell 49:487-498(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DEUBIQUITINATION OF FZO1, INTERACTION WITH FZO1.

    Entry informationi

    Entry nameiUBP12_YEAST
    AccessioniPrimary (citable) accession number: P39538
    Secondary accession number(s): D6VVZ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 7110 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3