P39538 (UBP12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 12
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 12
Ubiquitin thioesterase 12
Ubiquitin-specific-processing protease 12

Gene names

Name:	UBP12
Ordered Locus Names:	YJL197W
ORF Names:	J0340

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1254 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ubiquitin carboxyl-terminal hydrolase that recognizes ubiquitin chains that stabilize FZO1 and promote mitochondrial fusion. UBP12 deubiquitylates FZO1 only after oligomerization. Ref.6
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Interacts with FZO1. Ref.6
Miscellaneous	Present with 7110 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 DUSP domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro ubiquitin-specific protease activity Traceable author statement PubMed 8982460. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1254	1254	Ubiquitin carboxyl-terminal hydrolase 12		PRO_0000080597
Regions
	Domain	97 – 199	103	DUSP
Sites
	Active site	373	1	Nucleophile By similarity
	Active site	1068	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	12	1	Phosphoserine Ref.5
	Modified residue	84	1	Phosphoserine Ref.5
	Modified residue	86	1	Phosphothreonine Ref.5
	Modified residue	430	1	Phosphoserine Ref.5
	Modified residue	1153	1	Phosphothreonine Ref.5
	Modified residue	1226	1	Phosphoserine Ref.5
	Modified residue	1227	1	Phosphothreonine Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P39538 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 349AFA4C4CEE0EA5	FASTA	1,254	143,192
	10 20 30 40 50 60 MGSSDVSSRE CSLVYNEDPD FTDGTTPCDR LGVDLMNVLD DKDEIKQESV PVSDREIEDT 70 80 90 100 110 120 ESDASAVSSF ASANELIAEP HAASETNLGT NGQDGRNVLE QQRDVVARLI EENKETQKEG 130 140 150 160 170 180 DKVCIVPKVW YDKFFDPDVT DPEDIGPINT RMICRDFENF VLEDYNRCPY LSIAEPVFNF 190 200 210 220 230 240 LSEIYGMTSG SYPVVTNLVI NQTTGELETE YNKWFFRLHY LTEKQDGRKR RHGQDDSIMY 250 260 270 280 290 300 LSMSALNLVR DLVEKSMNLF FEKADHLDVN AVDFKIWFVS EGSDIATDSN VSTFLNSSYE 310 320 330 340 350 360 ITPLQFLELP IKKLLIPDMF ENRLDKITSN PSDLVIEIKP IEGNHHWPSN YFAYNKLEPA 370 380 390 400 410 420 SGTTGLVNLG NTCYMNSALQ CLVHIPQLRD YFLYDGYEDE INEENPLGYH GYVARAFSDL 430 440 450 460 470 480 VQKLFQNRMS IMQRNAAFPP SMFKSTIGHF NSMFSGYMQQ DSQEFLAFLL DSLHEDLNRI 490 500 510 520 530 540 IKKEYTEKPS LSPGDDVNDW NVVKKLADDT WEMHLKRNCS VITDLFVGMY KSTLYCPECQ 550 560 570 580 590 600 NVSITFDPYN DVTLPLPVDT VWDKTIKIFP MNSPPLLLEV ELSKSSTYMD LKNYVGKMSG 610 620 630 640 650 660 LDPNTLFGCE IFSNQIYVNY ESTESNAQFL TLQELIKPAD DVIFYELPVT NDNEVIVPVL 670 680 690 700 710 720 NTRIEKGYKN AMLFGVPFFI TLKEDELNNP GAIRMKLQNR FVHLSGGYIP FPEPVGNRTD 730 740 750 760 770 780 FADAFPLLVE KYPDVEFEQY KDILQYTSIK VTDKDKSFFS IKILSVEKEQ QFASNNRTGP 790 800 810 820 830 840 NFWTPISQLN LDKATDIDDK LEDVVKDIYN YSSLVDCAEG VLMQVDDEGD TEGSEAKNFS 850 860 870 880 890 900 KPFQSGDDEE NKETVTNNEN VNNTNDRDED MELTDDVEED ASTEPELTDK PEALDKIKDS 910 920 930 940 950 960 LTSTPFAILS MNDIIVCEWS ELGSNEAFSD DKIYNWENPA TLPNKELENA KLERSNAKER 970 980 990 1000 1010 1020 TITLDDCLQL FSKPEILGLT DSWYCPTCKE HRQATKQIQL WNTPDILLIH LKRFESQRSF 1030 1040 1050 1060 1070 1080 SDKIDATVNF PITDLDLSRY VVYKDDPRGL IYDLYAVDNH YGGLGGGHYT AYVKNFADNK 1090 1100 1110 1120 1130 1140 WYYFDDSRVT ETAPENSIAG SAYLLFYIRR HKDGNGLGSS KLQEIIQKSR HGYDERIKKI 1150 1160 1170 1180 1190 1200 YDEQMKLYEF NKTDEEEDVS DDMIECNEDV QAPEYSNRSL EVGHIETQDC NDEDDNDDGE 1210 1220 1230 1240 1250 RTNSGRRKLR LLKKVYKNNS GLGSSSTSEI SEGCPENEVA DLNLKNGVTL ESPE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes." Purnelle B., Coster F., Goffeau A. Yeast 10:1235-1249(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; SER-84; THR-86; SER-430; THR-1153; SER-1226 AND THR-1227, MASS SPECTROMETRY.
[6]	"Two deubiquitylases act on mitofusin and regulate mitochondrial fusion along independent pathways." Anton F., Dittmar G., Langer T., Escobar-Henriques M. Mol. Cell 49:487-498(2013) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN DEUBIQUITINATION OF FZO1, INTERACTION WITH FZO1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77688 Genomic DNA. Translation: CAA54762.1. Z49472 Genomic DNA. Translation: CAA89492.1. BK006943 Genomic DNA. Translation: DAA08611.1.
PIR	S46636.
RefSeq	NP_012338.1. NM_001181630.1.
3D structure databases
ProteinModelPortal	P39538.
SMR	P39538. Positions 362-561, 961-1110.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6312N.
IntAct	P39538. 6 interactions.
MINT	MINT-704453.
STRING	4932.YJL197W.
Protein family/group databases
MEROPS	C19.103.
Proteomic databases
PaxDb	P39538.
PeptideAtlas	P39538.
PRIDE	P39538.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL197W; YJL197W; YJL197W.
GeneID	853242.
KEGG	sce:YJL197W.
Organism-specific databases
CYGD	YJL197w.
SGD	S000003733. UBP12.
Phylogenomic databases
eggNOG	COG5560.
GeneTree	ENSGT00670000097750.
HOGENOM	HOG000057142.
KO	K11835.
OMA	WYHTFTI.
OrthoDB	EOG4FR40X.
Gene expression databases
Genevestigator	P39538.
GermOnline	YJL197W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006615. Pept_C19_DUSP. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
SMART	SM00695. DUSP. 1 hit. [Graphical view]
PROSITE	PS51283. DUSP. 1 hit. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973472.

Entry information

Entry name

UBP12_YEAST

Accession

Primary (citable) accession number: P39538
Secondary accession number(s): D6VVZ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program