Low-affinity phosphate transporter PHO90 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P39535 (PHO90_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 97. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Low-affinity phosphate transporter PHO90

Gene names

Name:	PHO90
Ordered Locus Names:	YJL198W
ORF Names:	J0336

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	881 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Low-affinity phosphate transporter involved in the control of cellular phosphate levels. Ref.4 Ref.7
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Induction	Expression is constitutive and independent of inorganic phosphate concentration and PHO4 activity. Ref.5
Miscellaneous	Present with 3430 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the CitM (TC 2.A.11) transporter family. [View classification] Contains 1 SPX domain.

Ontologies

Keywords
Biological process	Phosphate transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	sodium ion transport Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Molecular_function	phosphate ion transmembrane transporter activity Inferred from genetic interaction Ref.4. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 881

881

Low-affinity phosphate transporter PHO90

PRO_0000172518

Regions

Transmembrane

417 – 437

Helical; Potential

Transmembrane

456 – 476

Helical; Potential

Transmembrane

493 – 513

Helical; Potential

Transmembrane

514 – 534

Helical; Potential

Transmembrane

539 – 559

Helical; Potential

Transmembrane

581 – 601

Helical; Potential

Transmembrane

663 – 683

Helical; Potential

Transmembrane

691 – 711

Helical; Potential

Transmembrane

718 – 738

Helical; Potential

Transmembrane

758 – 778

Helical; Potential

Transmembrane

805 – 825

Helical; Potential

Transmembrane

854 – 874

Helical; Potential

Domain

1 – 288

288

SPX

Sequences

Sequence

Length

Mass (Da)

Tools

P39535 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: BB4466BC4D176714
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FASTA

881

97,689

        10         20         30         40         50         60 
MRFSHFLKYN AVPEWQNHYM DYSELKNLIY TLQTDELQVG DNEEGFGAGK SSNITDRFKN 

        70         80         90        100        110        120 
KFSFKNAKED TSSGMNKDAG IVEETIELRE LPTAQTVAAK PSPFRRMKEK IFYKRRSSSA 

       130        140        150        160        170        180 
SSVSSTANEN LQLDTYDTFV GDLTAEKQKV DDFYKRTEAK FYDKFDALVK DLKKIGVIEY 

       190        200        210        220        230        240 
DIDDDTLFNE PIASTNDEVP PLDLDDDEDD DEFYDDQSNI EDNTALLHHS QYNIKSQKKS 

       250        260        270        280        290        300 
LLKKSIVNLY IDLCQLKSFI ELNRIGFAKI TKKSDKVLHL NTRTELIESE QFFKDTYAFQ 

       310        320        330        340        350        360 
AETIELLNSK ISQLVTFYAR ITDRPHNISH SKQELKSYLH DHIVWERSNT WKDMLGLLSQ 

       370        380        390        400        410        420 
ADELTPKETE YNANKLVGKL DLEYYRWPLP RPINLKFTSI NNVALPKLFF TKKAYKIYFI 

       430        440        450        460        470        480 
ILVTGLLLGI KTFNDAAQHR CMALVECVAF LWASEAIPLH ITAFLVPLLV VLFKVLKTSD 

       490        500        510        520        530        540 
GAIMSAASAS SEILAAMWSS TIMILLAGFT LGEVLAQYNI AKVLASWLLA FAGCKPRNVL 

       550        560        570        580        590        600 
LMAMCVVFFL SMWISNVAAP VLTYSLLSPL LDAMDADSPF AQALVLGVAL AANIGGMSSP 

       610        620        630        640        650        660 
ISSPQNIISM SYLKPYGIGW GQFFAVALPS GILAMLLVWI LLFTTFKMNK TKLEKFKPIK 

       670        680        690        700        710        720 
TKFTVKQYYI ITVTVATILL WCVESQIEGA FGSSGQIAII PIVLFFGTGL LSTQDLNAFP 

       730        740        750        760        770        780 
WSIVILAMGG IALGKAVSSS GLLSTIAKAL QKKIENDGVF AILCIFGILM LVVGTFVSHT 

       790        800        810        820        830        840 
VSAIIIIPLV QEVGDKLGNP KAAPILVFGC ALLSSCGMGL ASSGFPNVTA ISKVDRKGDR 

       850        860        870        880 
YLSVMTFLTR GVPASILAFL CVITLGYGIM ASVVKGNATS A

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes." Purnelle B., Coster F., Goffeau A. Yeast 10:1235-1249(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Phosphate transport and sensing in Saccharomyces cerevisiae." Wykoff D.D., O'Shea E.K. Genetics 159:1491-1499(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Transcriptional regulation of phosphate-responsive genes in low-affinity phosphate-transporter-defective mutants in Saccharomyces cerevisiae." Auesukaree C., Homma T., Kaneko Y., Harashima S. Biochem. Biophys. Res. Commun. 306:843-850(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Transport and signaling through the phosphate-binding site of the yeast Pho84 phosphate transceptor." Popova Y., Thayumanavan P., Lonati E., Agrochao M., Thevelein J.M. Proc. Natl. Acad. Sci. U.S.A. 107:2890-2895(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77688 Genomic DNA. Translation: CAA54759.1. Z49473 Genomic DNA. Translation: CAA89493.1. BK006943 Genomic DNA. Translation: DAA08610.1.
PIR	S46633.
RefSeq	NP_012337.1. NM_001181631.1.
3D structure databases
ProteinModelPortal	P39535.
SMR	P39535. Positions 411-871.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4491371.
STRING	4932.YJL198W.
Proteomic databases
PaxDb	P39535.
PRIDE	P39535.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL198W; YJL198W; YJL198W.
GeneID	853241.
KEGG	sce:YJL198W.
Organism-specific databases
CYGD	YJL198w.
SGD	S000003734. PHO90.
Phylogenomic databases
eggNOG	COG5408.
GeneTree	ENSGT00550000074480.
KO	K14430.
OrthoDB	EOG437VP2.
Gene expression databases
Genevestigator	P39535.
GermOnline	YJL198W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001898. Na/sul_symport. IPR004331. SPX_N. [Graphical view]
Pfam	PF00939. Na_sulph_symp. 1 hit. PF03105. SPX. 1 hit. [Graphical view]
PROSITE	PS51382. SPX. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973469.

Entry information

Entry name

PHO90_YEAST

Accession

Primary (citable) accession number: P39535

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents