P39531 (RCY1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Recyclin-1

Gene names

Name:	RCY1
Ordered Locus Names:	YJL204C
ORF Names:	J0320, J0318

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	840 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in recycling plasma membrane proteins internalized by endocytosis. Required for recycling of the v-SNARE SNC1. Ref.5 Ref.6
Subunit structure	Interacts with SKP1. Ref.6
Subcellular location	Cytoplasm. Bud neck. Cell tip. Note: In unpolarized G1 cells this protein is found in patches in the cytoplasm, while after bud emergence it is concentrated in nascent buds and at the mother-bud neck. Accumulates at the shmoo tips of cells treated with pheromone. Localization depends on an intact actin cytoskeleton and a functional secretory pathway. Ref.6
Miscellaneous	Present with 2700 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Contains 1 F-box domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	early endosome to Golgi transport Inferred from mutant phenotype. Source: SGD endocytosis Inferred from mutant phenotype Ref.5. Source: SGD exocytosis Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle docking Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from direct assay. Source: SGD cell tip Inferred from electronic annotation. Source: UniProtKB-SubCell cellular bud neck Inferred from electronic annotation. Source: UniProtKB-SubCell endosome Inferred from direct assay. Source: SGD
Molecular function	SNARE binding Inferred from physical interaction. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 840	840	Recyclin-1		PRO_0000119953
Regions
	Domain	1 – 48	48	F-box
Amino acid modifications
	Modified residue	407	1	Phosphoserine Ref.8 Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P39531 [UniParc]. Last modified December 7, 2004. Version 4. Checksum: E8CDB82E00490886	FASTA	840	97,471
	10 20 30 40 50 60 MDDLLKVPEI VTNIASYLST VDYLSFQQVN KRVYAIINGK NDSKYWSLKL TRMGLQQVHS 70 80 90 100 110 120 NEEEEITLLD ENDNQNSLRI FEIYKSFTAQ NSKKIFVKFY RCYNSYARKL YNNNLANFFP 130 140 150 160 170 180 TSYSNDPLKQ TRILNFIKKY NFSNKNDIET FTRIETNFNI LREIFINSVL KESELNYQSN 190 200 210 220 230 240 NLAAVARFMK ILLISNEESN AIEFFKSKAD LPPSLTVLPS NDELFWAEQP REEDSGGSTV 250 260 270 280 290 300 IFNSKNLDTF LNQLRDFLNE KIKLADILFK DEFPVILQFI ESFIQDILLD ILNNILLSYS 310 320 330 340 350 360 EFLKENGKDS KANYECVPEL YFTFIKKFDT ELNDSVNAGA NFRKVVRDLL NLYLEPFVVN 370 380 390 400 410 420 YMNQTTRVFE SLINSQLANY DTQVQDKQRE QNAKIYNTLK DQTDASSASN NELPNDLSII 430 440 450 460 470 480 TETSKTVPEA DSKPSTIHQS VHSTDISNDK LDFLSSFTKI FKFSNNENQR LKQQLQLAYN 490 500 510 520 530 540 LNLISNNLQN IKSLISLDLC YKILQETSEK TDQIYKFHTI ESLLPLIKLR CQEIFKILIT 550 560 570 580 590 600 QLNKNHVKPA FEKAILLLQK YNPNEIEQIE IKFNSLSPAN TQVEPLVQFT ELINIGDIIL 610 620 630 640 650 660 QMISIFYKNE LIPKKIIDKN KDFLNDVIQL KKNFETSIDD FVAEGLNIGI NKLMDEISFV 670 680 690 700 710 720 FKTLQLPDDY NPPPPSRNSP IRDIKPTKCA IRVVELLSNH CFLLTGATDK GTIDVYQQEI 730 740 750 760 770 780 GERFFNEIVK HLKKCFISTE GAIWLICDLN YFYDFIANKL KQKNVVPYFV GLKSIGQLYI 790 800 810 820 830 840 ISGKDSKELG KLISDLGKFN GIFTQEEIYE FVQRRSDWVR VRKDVEKVMY GLGIRDCCIM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes." Purnelle B., Coster F., Goffeau A. Yeast 10:1235-1249(1994) [PubMed: 7754713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	Purnelle B. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The F-box protein Rcy1p is involved in endocytic membrane traffic and recycling out of an early endosome in Saccharomyces cerevisiae." Wiederkehr A., Avaro S., Prescianotto-Baschong C., Haguenauer-Tsapis R., Riezman H. J. Cell Biol. 149:397-410(2000) [PubMed: 10769031] [Abstract] Cited for: FUNCTION.
[6]	"Skp1p and the F-box protein Rcy1p form a non-SCF complex involved in recycling of the SNARE Snc1p in yeast." Galan J.M., Wiederkehr A., Seol J.H., Haguenauer-Tsapis R., Deshaies R.J., Riezman H., Peter M. Mol. Cell. Biol. 21:3105-3117(2001) [PubMed: 11287615] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SKP1.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-407, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-407, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77688 Genomic DNA. Translation: CAA54753.1. Z49479 Genomic DNA. Translation: CAA89499.1. Z49480 Genomic DNA. Translation: CAA89501.1. BK006943 Genomic DNA. Translation: DAA08606.1.
PIR	S77615.
RefSeq	NP_012331.1. NM_001181637.1.
3D structure databases
ProteinModelPortal	P39531.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7247N.
IntAct	P39531. 1 interaction.
STRING	P39531.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL204C; YJL204C; YJL204C.
GeneID	853226.
KEGG	sce:YJL204C.
NMPDR	fig|4932.3.peg.3294.
Organism-specific databases
CYGD	YJL204c.
SGD	S000003740. RCY1.
Phylogenomic databases
eggNOG	fuNOG07204.
GeneTree	EFGT00050000004903.
HOGENOM	HBG202213.
OMA	FTQEEIY.
OrthoDB	EOG4MGWGZ.
Gene expression databases
ArrayExpress	P39531.
Genevestigator	P39531.
GermOnline	YJL204C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR009976. Sec10. [Graphical view]
KO	K15071.
Pfam	PF07393. Sec10. 1 hit. [Graphical view]
PROSITE	PS50181. FBOX. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	973434.

Entry information

Entry name

RCY1_YEAST

Accession

Primary (citable) accession number: P39531
Secondary accession number(s): D6VVZ0, P39530, P87193

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 7, 2004
Last modified:	December 14, 2011
This is version 84 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program