P39526 (LAA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AP-1 accessory protein LAA1

Alternative name(s):
Large AP-1 accessory protein 1

Gene names

Name:	LAA1
Ordered Locus Names:	YJL207C
ORF Names:	J0312, HRD550

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	2014 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the trans-Golgi network (TGN)-endosome transport. Important for the correct localization of the adapter protein complex AP-1. Ref.9
Subunit structure	Interacts with the clathrin-associated adapter complex AP-1. Ref.9
Subcellular location	Golgi apparatus. Note: Colocalizes with AP-1 adapter complex, with clathrin-coated vesicles to the late-Golgi apparatus and with ribosomes. Localization depends on ADP-ribosylation factor (ARF). Ref.6 Ref.8 Ref.9
Disruption phenotype	Deletion, when combined with a conditional mutation in clathrin heavy chain or deletion of GGA genes, accentuates growth defects and increases disruption of clathrin-dependent alpha-factor maturation and transport of carboxypeptidase Y to the vacuole. Causes mislocalization of AP-1, especially in cells at high density (postdiauxic shift), but doesn't affect GGA protein distribution. Sensitive to diltiazem-HCl and hypersensitive to chlorpromazine. Ref.9
Miscellaneous	Present with 300 molecules/cell in log phase SD medium. Ref.7

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW retrograde transport, endosome to Golgi Inferred from mutant phenotype Ref.9. Source: SGD
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2014	2014	AP-1 accessory protein LAA1		PRO_0000203015

Sequences

	Sequence		Length	Mass (Da)
	P39526 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 6A296796F2FA5791	FASTA	2,014	229,900
	10 20 30 40 50 60 MANRSLKKVI ETSSNNGHDL LTWITTNLEK LICLKEVNDN EIQEVKEIHT QLDEFVRYIS 70 80 90 100 110 120 VLENTDDLEL HSVFISLSQL YTISIWRLKD EYPGVVFDSA AFLTNVLCEE DVSIDDGDTD 130 140 150 160 170 180 PNQKKKKKKS STKKKKYIYS PAKDIACTIL VQLFENFGSS ISSLIPLLFN AIFKNLKKIM 190 200 210 220 230 240 EKSKYYHATF MTTLLQLFNA ILRNSNNDDK ILDPATYAKF SKLSKTVFDS ISTDEKDFSV 250 260 270 280 290 300 TFVSVLIECW TAHFKQTNFI REHSHDIIET IYSRFTEGEI GVYGFANDET RIFTAKSLAE 310 320 330 340 350 360 ILFDYYFSKN ILTLQEVWSI YVKIFLNCDT RDVESGCFES IIHLINLNLL ADNTFLSNSK 370 380 390 400 410 420 YLDIVLSLSG VFSSYEVNNR SMNTLSRYLR YFQHMHEVIL PHLNDSAKTQ MLYYILGCSD 430 440 450 460 470 480 TYQSSSKSDS ASNFKYSIDA KPETQWLTLL QLDFTYVLIS DLGSTFTTEE NTVKEIRDKL 490 500 510 520 530 540 VDLATCEIFT IRVHTVEILK VFLNNCPEYL SETIENSLRA LSTDFKSTGK FIFHKNHGHA 550 560 570 580 590 600 FIIANLIKGA ESDYISYELI MRITVFSTSF IKNNTTSTSS NLYFKGLLCW ILLIGLMNYK 610 620 630 640 650 660 DEQYLKLQIP QLFLFWKVLL THTYTYHDED ELYKNLEIRN HALTCLLTYL SNTTIDKEMA 670 680 690 700 710 720 KQVSYLLTKC SNFNHSIDLK SKNIDNALLH NENRILQVYL KLEKYINSDF NSSLLILIVK 730 740 750 760 770 780 NFSDPNLYTE SSSSVLGSLK DIGNRKVSNK DDMESNIVLE SSINTLLRQN NGFAFGLSSK 790 800 810 820 830 840 ITGDRIVNLS MSSAYKYDES ISGSWPSKDY NWYNIFEVEV SKPISPILSL DSLILLYGSG 850 860 870 880 890 900 SYSQIDRYAP QVTTSLIDSS MELFSSVFPF LNSKIQYSIM ETLNLSMFSK MTTPLRSVAV 910 920 930 940 950 960 AANVCSALHN ALRIMQENNL ELDYSVGQLI IESIKKIQFF NDIFLTKIKA DCVGLLTAAI 970 980 990 1000 1010 1020 ARTLGDEERQ KFLTEQSRIF IKNVADMDEP YLRMFHVLSL ATIFKYNSQY ANFEEYFDVI 1030 1040 1050 1060 1070 1080 FALMRDPHPV VHSWSLKAMH ILLEKHLVID LKTAALLLSS MEELLVQDKY GIYGRSTLRC 1090 1100 1110 1120 1130 1140 NYNRDFNSHV AIGEISRTLT ETVGPNFLEL NTKVLDSFRN ITLSMLISNN ILNSITSIKM 1150 1160 1170 1180 1190 1200 FENIATFKMK NILNYEIFIL ASKSIIKSSI VTGIGSSYFD TTFTGSNELI SRTSSLKGAF 1210 1220 1230 1240 1250 1260 ENFDLLTLLY KLQMEEFFMK EMENLSWRYL ALFPNSGSVK NYFTEWILHT FKRDNHWFDK 1270 1280 1290 1300 1310 1320 LYSIFNMSLG RLFQSYNRDV SALLEVNGLK KSSEKEIKGE EEESIANVNQ LTDTDAGGLD 1330 1340 1350 1360 1370 1380 SENLQWKSRQ IILNLILMLC LESEKYENLL LALSNKIADL IKISFRGSTV RNEGMKLTGL 1390 1400 1410 1420 1430 1440 HILNFVLKNY STMRDPQVPG SSILEQQEAQ ITSALMPAFS KGSSPTVMSF AITVAAEVLA 1450 1460 1470 1480 1490 1500 SNIMPPDKLG RISQLLIDLL GNFKDPNSGI RIGEAIIVTP KAKRKIELAV LDAWAEVVQR 1510 1520 1530 1540 1550 1560 SITSSNDALF SFTRKYWSIL VPLWIISLRE YMMIKYNDND STVQVKNDSK ENSLIEPRST 1570 1580 1590 1600 1610 1620 KIELYEPVWL NFVEALGCTL DSDVQVILAS LNDEELEYFL FILFSQCLEA IVKNIDDHSV 1630 1640 1650 1660 1670 1680 KMQVLPALHN VLKSNLCIKS IFEDDIITEV VEIMDRLIST GDSKEEFLLV DIISDLIIGY 1690 1700 1710 1720 1730 1740 SKCNATPETF LQDIDKLYEL LRLLMTIISE RLPFIKYNVL TSEEDDNEIK ISPTDISLLK 1750 1760 1770 1780 1790 1800 KTFIAFESNI SNFDNMFKVD LYSCLLFIIG KIYECSHREV IIPIILPLFK ALVKALTESE 1810 1820 1830 1840 1850 1860 DEKNIVLLEI FYGSIKDVIY HKLDSKNKVA TILILLSNGY SKLSFQELNQ CANILSEALN 1870 1880 1890 1900 1910 1920 NPATQPIALQ GFKRIISNIF KYPLLQYFMK LVIKRFFQDI QTNDSLSQAS IKTKLIIQFS 1930 1940 1950 1960 1970 1980 EEVIKQDHQK ASLSIALCLS FFAAYHSAYT EKIDNEVASG IVALAKLDKN SFKEAISSTI 1990 2000 2010 SPQQKAIIGS VMEAYVKSQS LGSVEEAFQL KSFD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes." Purnelle B., Coster F., Goffeau A. Yeast 10:1235-1249(1994) [PubMed: 7754713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Sequence analysis of a 40.2 kb DNA fragment located near the left telomere of yeast chromosome X." Vandenbol M., Durand P., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:1657-1662(1994) [PubMed: 7725802] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1465-2014. Strain: ATCC 204508 / S288c.
[5]	"Chemotyping of yeast mutants using robotics." Rieger K.-J., El-Alama M., Stein G., Bradshaw C., Slonimski P.P., Maundrell K. Yeast 15:973-986(1999) [PubMed: 10407277] [Abstract] Cited for: DRUG SENSITIVITY.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Systematic identification and functional screens of uncharacterized proteins associated with eukaryotic ribosomal complexes." Fleischer T.C., Weaver C.M., McAfee K.J., Jennings J.L., Link A.J. Genes Dev. 20:1294-1307(2006) [PubMed: 16702403] [Abstract] Cited for: SUBCELLULAR LOCATION.
[9]	"Laa1p, a conserved AP-1 accessory protein important for AP-1 localization in yeast." Fernandez G.E., Payne G.S. Mol. Biol. Cell 17:3304-3317(2006) [PubMed: 16687571] [Abstract] Cited for: FUNCTION, INTERACTION WITH AP-1, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77688 Genomic DNA. Translation: CAA54749.1. Z34098 Genomic DNA. Translation: CAA84004.1. Z49482 Genomic DNA. Translation: CAA89504.1. BK006943 Genomic DNA. Translation: DAA08603.1.
PIR	S46622.
RefSeq	NP_012328.1. NM_001181640.1.
3D structure databases
ProteinModelPortal	P39526.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6552N.
IntAct	P39526. 21 interactions.
MINT	MINT-686856.
STRING	P39526.
Proteomic databases
PeptideAtlas	P39526.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL207C; YJL207C; YJL207C.
GeneID	853223.
KEGG	sce:YJL207C.
NMPDR	fig|4932.3.peg.3291.
Organism-specific databases
CYGD	YJL207c.
SGD	S000003743. LAA1.
Phylogenomic databases
eggNOG	fuNOG04570.
GeneTree	EFGT00050000003863.
HOGENOM	HBG202196.
OMA	WEFKLFI.
OrthoDB	EOG42NN7D.
Gene expression databases
ArrayExpress	P39526.
Genevestigator	P39526.
GermOnline	YJL207C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 5 hits.
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
ProtoNet	Search...
Other
NextBio	973425.

Entry information

Entry name

LAA1_YEAST

Accession

Primary (citable) accession number: P39526
Secondary accession number(s): D6VVY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program