P39525 (CEM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase homolog
EC=2.3.1.41

Alternative name(s):
Beta-ketoacyl-ACP synthase homolog

Gene names

Name:	CEM1
Ordered Locus Names:	YER061C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Possibly involved in the synthesis of a specialized molecule, probably related to a fatty acid, which is essential for mitochondrial respiration. Is essential for oxygen uptake and the presence of cytochromes A and B.
Catalytic activity	Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].
Subcellular location	Mitochondrion Ref.4.
Miscellaneous	Present with 1660 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the beta-ketoacyl-ACP synthases family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Cellular component	Mitochondrion
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from sequence alignment PubMed 15668256. Source: SGD
Cellular_component	mitochondrion Inferred from direct assay Ref.4PubMed 16823961. Source: SGD
Molecular_function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from sequence alignment PubMed 15668256. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	3-oxoacyl-[acyl-carrier-protein] synthase homolog		PRO_0000180356
Sites
	Active site	187	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39525 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: DAF2FF358A7AA067	FASTA	442	47,555
	10 20 30 40 50 60 MSRRVVITGL GCVTPLGRSL SESWGNLLSS KNGLTPITSL PNYNEDYKLR EKSIPSTITV 70 80 90 100 110 120 GKIPENFQNE NSAINKLLFT SQDERRTSSF IKLALRTTYE ALHNAGLLNP NDITINTSLC 130 140 150 160 170 180 NLDHFGCLIG SGIGSIQDIY QTSLQFHNDN KRINPYFVPK ILTNMAAGNV SIKFNLRGLS 190 200 210 220 230 240 HSVSTACATG NNSIGDAFNF IRLGMQDICV AGASETSLHP LSLAGFIRAK SITTNGISRP 250 260 270 280 290 300 FDTQRSGFVL GEGCGMIVME SLEHAQKRNA NIISELVGYG LSSDACHITS PPADGNGAKR 310 320 330 340 350 360 AIEMALKMAR LEPTDVDYVN AHATSTLLGD KAECLAVASA LLPGRSKSKP LYISSNKGAI 370 380 390 400 410 420 GHLLGAAGAV ESIFTICSLK DDKMPHTLNL DNVLTLENNE ADKLHFIRDK PIVGANPKYA 430 440 LCNSFGFGGV NTSLLFKKWE GS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a new nuclear gene (CEM1) encoding a protein homologous to a beta-keto-acyl synthase which is essential for mitochondrial respiration in Saccharomyces cerevisiae." Harington A., Herbert C.J., Tung B., Getz G.S., Slonimski P.P. Mol. Microbiol. 9:545-555(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CW04.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X73488 Genomic DNA. Translation: CAB58180.1. U18813 Genomic DNA. Translation: AAB64597.1. BK006939 Genomic DNA. Translation: DAA07720.2.
PIR	S36204.
RefSeq	NP_010983.2. NM_001178952.2.
3D structure databases
ProteinModelPortal	P39525.
SMR	P39525. Positions 3-436.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5342N.
IntAct	P39525. 1 interaction.
MINT	MINT-515613.
STRING	4932.YER061C.
Proteomic databases
PaxDb	P39525.
PeptideAtlas	P39525.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YER061C; YER061C; YER061C.
GeneID	856790.
KEGG	sce:YER061C.
Organism-specific databases
SGD	S000000863. CEM1.
Phylogenomic databases
eggNOG	COG0304.
GeneTree	ENSGT00530000063309.
HOGENOM	HOG000060166.
KO	K09458.
OMA	MSSPTIM.
OrthoDB	EOG44N21Z.
Enzyme and pathway databases
BioCyc	YEAST:YER061C-MONOMER.
Gene expression databases
Genevestigator	P39525.
GermOnline	YER061C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.47.10. 2 hits.
InterPro	IPR017568. 3-oxoacyl-ACP_synth-2. IPR018201. Ketoacyl_synth_AS. IPR014031. Ketoacyl_synth_C. IPR014030. Ketoacyl_synth_N. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF00109. ketoacyl-synt. 1 hit. PF02801. Ketoacyl-synt_C. 1 hit. [Graphical view]
PIRSF	PIRSF000447. KAS_II. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 1 hit.
PROSITE	PS00606. B_KETOACYL_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	983018.

Entry information

Entry name

CEM1_YEAST

Accession

Primary (citable) accession number: P39525
Secondary accession number(s): D3DLW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program