Reviewed, UniProtKB/Swiss-Prot P39525 (CEM1_YEAST)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] synthase homolog
    EC=2.3.1.41
Alternative name(s):
    Beta-ketoacyl-ACP synthase homolog

Gene names

Name:	CEM1
Ordered Locus Names:	YER061C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Possibly involved in the synthesis of a specialized molecule, probably related to a fatty acid, which is essential for mitochondrial respiration. Is essential for oxygen uptake and the presence of cytochromes A and B.
Catalytic activity	Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].
Subcellular location	Mitochondrion Potential.
Miscellaneous	Present with 1660 molecules/cell in log phase SD medium. Ref.3
Sequence similarities	Belongs to the beta-ketoacyl-ACP synthases family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Mitochondrion
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: EC protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38799	1	EBI-4467,EBI-24593
	P38800	1	EBI-4467,EBI-24597
MGM1	P32266	1	EBI-4467,EBI-10865
UBC13	P52490	1	EBI-4467,EBI-19777

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	3-oxoacyl-[acyl-carrier-protein] synthase homolog		PRO_0000180356
Sites
	Active site	187	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39525-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: DAF2FF358A7AA067	FASTA	442	47,555
	10 20 30 40 50 60 MSRRVVITGL GCVTPLGRSL SESWGNLLSS KNGLTPITSL PNYNEDYKLR EKSIPSTITV 70 80 90 100 110 120 GKIPENFQNE NSAINKLLFT SQDERRTSSF IKLALRTTYE ALHNAGLLNP NDITINTSLC 130 140 150 160 170 180 NLDHFGCLIG SGIGSIQDIY QTSLQFHNDN KRINPYFVPK ILTNMAAGNV SIKFNLRGLS 190 200 210 220 230 240 HSVSTACATG NNSIGDAFNF IRLGMQDICV AGASETSLHP LSLAGFIRAK SITTNGISRP 250 260 270 280 290 300 FDTQRSGFVL GEGCGMIVME SLEHAQKRNA NIISELVGYG LSSDACHITS PPADGNGAKR 310 320 330 340 350 360 AIEMALKMAR LEPTDVDYVN AHATSTLLGD KAECLAVASA LLPGRSKSKP LYISSNKGAI 370 380 390 400 410 420 GHLLGAAGAV ESIFTICSLK DDKMPHTLNL DNVLTLENNE ADKLHFIRDK PIVGANPKYA 430 440 LCNSFGFGGV NTSLLFKKWE GS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a new nuclear gene (CEM1) encoding a protein homologous to a beta-keto-acyl synthase which is essential for mitochondrial respiration in Saccharomyces cerevisiae." Harington A., Herbert C.J., Tung B., Getz G.S., Slonimski P.P. Mol. Microbiol. 9:545-555(1993) [PubMed: 8412701] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CW04.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome V." Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W. Nature 387:78-81(1997) [PubMed: 9169868] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X73488 Genomic DNA. Translation: CAB58180.1. U18813 Genomic DNA. Translation: AAB64597.1.
PIR	S36204.
3D structure databases
HSSP	HSSP built from PDB template 1KAS based on UniProtKB P39435.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5342N.
IntAct	P39525. 7 interactions.
STRING	P39525.
Proteomic databases
PeptideAtlas	P39525.
Genome annotation databases
Ensembl	YER061C; YER061C; YER061C; Saccharomyces cerevisiae. [Genome view]
GenomeReviews	Gene locus YER061C in contig U00092_GR.
Organism-specific databases
CYGD	YER061c.
SGD	S000000863. CEM1.
Phylogenomic databases
HOGENOM	P39525.
Enzyme and pathway databases
BRENDA	2.3.1.41. 250.
Gene expression databases
ArrayExpress	P39525.
Genevestigator	P39525.
GermOnline	YER061C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000794. Beta-ketoacyl_synthase. IPR018201. Ketoacyl_synth_AS. IPR014031. Ketoacyl_synth_C. IPR014030. Ketoacyl_synth_N. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
PANTHER	PTHR11712. Ketoacyl_synth. 1 hit.
Pfam	PF00109. ketoacyl-synt. 1 hit. PF02801. Ketoacyl-synt_C. 1 hit. [Graphical view]
PROSITE	PS00606. B_KETOACYL_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CEM1_YEAST

Accession

Primary (citable) accession number: P39525

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	October 13, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)