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P39524

- ATC3_YEAST

UniProt

P39524 - ATC3_YEAST

Protein

Probable phospholipid-transporting ATPase DRS2

Gene

DRS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids Potential. Seems to be involved in ribosome assembly.Curated

    Catalytic activityi

    ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei560 – 56014-aspartylphosphate intermediateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cation-transporting ATPase activity Source: InterPro
    3. magnesium ion binding Source: InterPro
    4. phospholipid-translocating ATPase activity Source: SGD

    GO - Biological processi

    1. endocytic recycling Source: SGD
    2. endocytosis Source: SGD
    3. intracellular protein transport Source: SGD
    4. phospholipid translocation Source: SGD
    5. post-Golgi vesicle-mediated transport Source: SGD
    6. ribosomal small subunit assembly Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28837-MONOMER.
    ReactomeiREACT_96459. Ion transport by P-type ATPases.

    Protein family/group databases

    TCDBi3.A.3.8.2. the p-type atpase (p-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable phospholipid-transporting ATPase DRS2 (EC:3.6.3.1)
    Gene namesi
    Name:DRS2
    Ordered Locus Names:YAL026C
    ORF Names:FUN38
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAL026c.
    SGDiS000000024. DRS2.

    Subcellular locationi

    Golgi apparatustrans-Golgi network membrane 2 Publications; Multi-pass membrane protein 2 Publications

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. trans-Golgi network Source: SGD

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13551355Probable phospholipid-transporting ATPase DRS2PRO_0000046233Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei102 – 1021Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39524.
    PaxDbiP39524.

    Expressioni

    Gene expression databases

    GenevestigatoriP39524.

    Interactioni

    Subunit structurei

    Interacts with CDC50.1 Publication

    Protein-protein interaction databases

    BioGridi31740. 190 interactions.
    DIPiDIP-2216N.
    IntActiP39524. 10 interactions.
    MINTiMINT-520976.
    STRINGi4932.YAL026C.

    Structurei

    3D structure databases

    ProteinModelPortaliP39524.
    SMRiP39524. Positions 216-1019.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 221221CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini243 – 2464LumenalSequence Analysis
    Topological domaini268 – 449182CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini471 – 49020LumenalSequence AnalysisAdd
    BLAST
    Topological domaini512 – 1012501CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1034 – 104310LumenalSequence Analysis
    Topological domaini1065 – 109430CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini1116 – 113116LumenalSequence AnalysisAdd
    BLAST
    Topological domaini1153 – 11619CytoplasmicSequence Analysis
    Topological domaini1183 – 120220LumenalSequence AnalysisAdd
    BLAST
    Topological domaini1224 – 1355132CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei222 – 24221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei247 – 26721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei450 – 47021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei491 – 51121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1013 – 103321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1044 – 106421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1095 – 111521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1132 – 115221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1162 – 118221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1203 – 122321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    GeneTreeiENSGT00740000115297.
    HOGENOMiHOG000202528.
    KOiK14802.
    OMAiIPRIYES.
    OrthoDBiEOG7FR7QR.

    Family and domain databases

    Gene3Di2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    3.40.50.1000. 2 hits.
    InterProiIPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR006539. ATPase_P-typ_Plipid-transp.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PANTHERiPTHR24092. PTHR24092. 1 hit.
    PfamiPF00122. E1-E2_ATPase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SUPFAMiSSF56784. SSF56784. 3 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
    TIGR01494. ATPase_P-type. 1 hit.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39524-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNDDRETPPK RKPGEDDTLF DIDFLDDTTS HSGSRSKVTN SHANANYIPP     50
    SHVLPEETID LDADDDNIEN DVHENLFMSN NHDDQTSWNA NRFDSDAYQP 100
    QSLRAVKPPG LFARFGNGLK NAFTFKRKKG PESFEMNHYN AVTNNELDDN 150
    YLDSRNKFNI KILFNRYILR KNVGDAEGNG EPRVIHINDS LANSSFGYSD 200
    NHISTTKYNF ATFLPKFLFQ EFSKYANLFF LCTSAIQQVP HVSPTNRYTT 250
    IGTLLVVLIV SAMKECIEDI KRANSDKELN NSTAEIFSEA HDDFVEKRWI 300
    DIRVGDIIRV KSEEPIPADT IILSSSEPEG LCYIETANLD GETNLKIKQS 350
    RVETAKFIDV KTLKNMNGKV VSEQPNSSLY TYEGTMTLND RQIPLSPDQM 400
    ILRGATLRNT AWIFGLVIFT GHETKLLRNA TATPIKRTAV EKIINRQIIA 450
    LFTVLIVLIL ISSIGNVIMS TADAKHLSYL YLEGTNKAGL FFKDFLTFWI 500
    LFSNLVPISL FVTVELIKYY QAFMIGSDLD LYYEKTDTPT VVRTSSLVEE 550
    LGQIEYIFSD KTGTLTRNIM EFKSCSIAGH CYIDKIPEDK TATVEDGIEV 600
    GYRKFDDLKK KLNDPSDEDS PIINDFLTLL ATCHTVIPEF QSDGSIKYQA 650
    ASPDEGALVQ GGADLGYKFI IRKPNSVTVL LEETGEEKEY QLLNICEFNS 700
    TRKRMSAIFR FPDGSIKLFC KGADTVILER LDDEANQYVE ATMRHLEDYA 750
    SEGLRTLCLA MRDISEGEYE EWNSIYNEAA TTLDNRAEKL DEAANLIEKN 800
    LILIGATAIE DKLQDGVPET IHTLQEAGIK IWVLTGDRQE TAINIGMSCR 850
    LLSEDMNLLI INEETRDDTE RNLLEKINAL NEHQLSTHDM NTLALVIDGK 900
    SLGFALEPEL EDYLLTVAKL CKAVICCRVS PLQKALVVKM VKRKSSSLLL 950
    AIGDGANDVS MIQAAHVGVG ISGMEGMQAA RSADIAVGQF KFLKKLLLVH 1000
    GSWSYQRISV AILYSFYKNT ALYMTQFWYV FANAFSGQSI MESWTMSFYN 1050
    LFFTVWPPFV IGVFDQFVSS RLLERYPQLY KLGQKGQFFS VYIFWGWIIN 1100
    GFFHSAIVFI GTILIYRYGF ALNMHGELAD HWSWGVTVYT TSVIIVLGKA 1150
    ALVTNQWTKF TLIAIPGSLL FWLIFFPIYA SIFPHANISR EYYGVVKHTY 1200
    GSGVFWLTLI VLPIFALVRD FLWKYYKRMY EPETYHVIQE MQKYNISDSR 1250
    PHVQQFQNAI RKVRQVQRMK KQRGFAFSQA EEGGQEKIVR MYDTTQKRGK 1300
    YGELQDASAN PFNDNNGLGS NDFESAEPFI ENPFADGNQN SNRFSSSRDD 1350
    ISFDI 1355
    Length:1,355
    Mass (Da):153,765
    Last modified:July 27, 2011 - v2
    Checksum:i5745B92901F8E1AE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti45 – 462AN → GY in AAA16891. (PubMed:8247005)Curated
    Sequence conflicti45 – 462AN → GY in AAC05006. (PubMed:7731988)Curated
    Sequence conflicti450 – 4501A → R in AAA16891. (PubMed:8247005)Curated
    Sequence conflicti450 – 4501A → R in AAC05006. (PubMed:7731988)Curated
    Sequence conflicti674 – 6741P → G in AAA16891. (PubMed:8247005)Curated
    Sequence conflicti674 – 6741P → G in AAC05006. (PubMed:7731988)Curated
    Sequence conflicti891 – 8922NT → KS in AAA16891. (PubMed:8247005)Curated
    Sequence conflicti891 – 8922NT → KS in AAC05006. (PubMed:7731988)Curated
    Sequence conflicti953 – 9542GD → AS in AAA16891. (PubMed:8247005)Curated
    Sequence conflicti953 – 9542GD → AS in AAC05006. (PubMed:7731988)Curated
    Sequence conflicti987 – 9871V → L in AAA16891. (PubMed:8247005)Curated
    Sequence conflicti987 – 9871V → L in AAC05006. (PubMed:7731988)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01795 Unassigned RNA. Translation: AAA16891.1.
    U12980 Genomic DNA. Translation: AAC05006.1.
    BK006935 Genomic DNA. Translation: DAA06962.2.
    PIRiS51995.
    RefSeqiNP_009376.2. NM_001178171.2.

    Genome annotation databases

    EnsemblFungiiYAL026C; YAL026C; YAL026C.
    GeneIDi851207.
    KEGGisce:YAL026C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L01795 Unassigned RNA. Translation: AAA16891.1 .
    U12980 Genomic DNA. Translation: AAC05006.1 .
    BK006935 Genomic DNA. Translation: DAA06962.2 .
    PIRi S51995.
    RefSeqi NP_009376.2. NM_001178171.2.

    3D structure databases

    ProteinModelPortali P39524.
    SMRi P39524. Positions 216-1019.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31740. 190 interactions.
    DIPi DIP-2216N.
    IntActi P39524. 10 interactions.
    MINTi MINT-520976.
    STRINGi 4932.YAL026C.

    Protein family/group databases

    TCDBi 3.A.3.8.2. the p-type atpase (p-atpase) superfamily.

    Proteomic databases

    MaxQBi P39524.
    PaxDbi P39524.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL026C ; YAL026C ; YAL026C .
    GeneIDi 851207.
    KEGGi sce:YAL026C.

    Organism-specific databases

    CYGDi YAL026c.
    SGDi S000000024. DRS2.

    Phylogenomic databases

    eggNOGi COG0474.
    GeneTreei ENSGT00740000115297.
    HOGENOMi HOG000202528.
    KOi K14802.
    OMAi IPRIYES.
    OrthoDBi EOG7FR7QR.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28837-MONOMER.
    Reactomei REACT_96459. Ion transport by P-type ATPases.

    Miscellaneous databases

    NextBioi 968076.
    PROi P39524.

    Gene expression databases

    Genevestigatori P39524.

    Family and domain databases

    Gene3Di 2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    3.40.50.1000. 2 hits.
    InterProi IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR006539. ATPase_P-typ_Plipid-transp.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    PANTHERi PTHR24092. PTHR24092. 1 hit.
    Pfami PF00122. E1-E2_ATPase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SUPFAMi SSF56784. SSF56784. 3 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01652. ATPase-Plipid. 1 hit.
    TIGR01494. ATPase_P-type. 1 hit.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "DRS1 to DRS7, novel genes required for ribosome assembly and function in Saccharomyces cerevisiae."
      Ripmaster T.L., Vaughn G.P., Woolford J.L. Jr.
      Mol. Cell. Biol. 13:7901-7912(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 45-46; 450; 674; 891-892; 953-954 AND 987.
      Strain: ATCC 204508 / S288c.
    4. "Drs2p-dependent formation of exocytic clathrin-coated vesicles in vivo."
      Gall W.E., Geething N.C., Hua Z., Ingram M.F., Liu K., Chen S.I., Graham T.R.
      Curr. Biol. 12:1623-1627(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.
    5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae."
      Saito K., Fujimura-Kamada K., Furuta N., Kato U., Umeda M., Tanaka K.
      Mol. Biol. Cell 15:3418-3432(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CDC50.
    8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiATC3_YEAST
    AccessioniPrimary (citable) accession number: P39524
    Secondary accession number(s): D6VPJ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 144 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 606 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3