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P39524

- ATC3_YEAST

UniProt

P39524 - ATC3_YEAST

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Protein

Probable phospholipid-transporting ATPase DRS2

Gene
DRS2, YAL026C, FUN38
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids Reviewed prediction. Seems to be involved in ribosome assembly.

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei560 – 56014-aspartylphosphate intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cation-transporting ATPase activity Source: InterPro
  3. magnesium ion binding Source: InterPro
  4. phospholipid-translocating ATPase activity Source: SGD

GO - Biological processi

  1. endocytic recycling Source: SGD
  2. endocytosis Source: SGD
  3. intracellular protein transport Source: SGD
  4. phospholipid translocation Source: SGD
  5. post-Golgi vesicle-mediated transport Source: SGD
  6. ribosomal small subunit assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28837-MONOMER.
ReactomeiREACT_96459. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.8.2. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid-transporting ATPase DRS2 (EC:3.6.3.1)
Gene namesi
Name:DRS2
Ordered Locus Names:YAL026C
ORF Names:FUN38
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL026c.
SGDiS000000024. DRS2.

Subcellular locationi

Golgi apparatustrans-Golgi network membrane; Multi-pass membrane protein 2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 221221Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei222 – 24221Helical; Reviewed predictionAdd
BLAST
Topological domaini243 – 2464Lumenal Reviewed prediction
Transmembranei247 – 26721Helical; Reviewed predictionAdd
BLAST
Topological domaini268 – 449182Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei450 – 47021Helical; Reviewed predictionAdd
BLAST
Topological domaini471 – 49020Lumenal Reviewed predictionAdd
BLAST
Transmembranei491 – 51121Helical; Reviewed predictionAdd
BLAST
Topological domaini512 – 1012501Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei1013 – 103321Helical; Reviewed predictionAdd
BLAST
Topological domaini1034 – 104310Lumenal Reviewed prediction
Transmembranei1044 – 106421Helical; Reviewed predictionAdd
BLAST
Topological domaini1065 – 109430Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei1095 – 111521Helical; Reviewed predictionAdd
BLAST
Topological domaini1116 – 113116Lumenal Reviewed predictionAdd
BLAST
Transmembranei1132 – 115221Helical; Reviewed predictionAdd
BLAST
Topological domaini1153 – 11619Cytoplasmic Reviewed prediction
Transmembranei1162 – 118221Helical; Reviewed predictionAdd
BLAST
Topological domaini1183 – 120220Lumenal Reviewed predictionAdd
BLAST
Transmembranei1203 – 122321Helical; Reviewed predictionAdd
BLAST
Topological domaini1224 – 1355132Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. trans-Golgi network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13551355Probable phospholipid-transporting ATPase DRS2PRO_0000046233Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei102 – 1021Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39524.
PaxDbiP39524.

Expressioni

Gene expression databases

GenevestigatoriP39524.

Interactioni

Subunit structurei

Interacts with CDC50.1 Publication

Protein-protein interaction databases

BioGridi31740. 189 interactions.
DIPiDIP-2216N.
IntActiP39524. 10 interactions.
MINTiMINT-520976.
STRINGi4932.YAL026C.

Structurei

3D structure databases

ProteinModelPortaliP39524.
SMRiP39524. Positions 216-1019.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
GeneTreeiENSGT00740000115297.
HOGENOMiHOG000202528.
KOiK14802.
OMAiIPRIYES.
OrthoDBiEOG7FR7QR.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR006539. ATPase_P-typ_Plipid-transp.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39524-1 [UniParc]FASTAAdd to Basket

« Hide

MNDDRETPPK RKPGEDDTLF DIDFLDDTTS HSGSRSKVTN SHANANYIPP     50
SHVLPEETID LDADDDNIEN DVHENLFMSN NHDDQTSWNA NRFDSDAYQP 100
QSLRAVKPPG LFARFGNGLK NAFTFKRKKG PESFEMNHYN AVTNNELDDN 150
YLDSRNKFNI KILFNRYILR KNVGDAEGNG EPRVIHINDS LANSSFGYSD 200
NHISTTKYNF ATFLPKFLFQ EFSKYANLFF LCTSAIQQVP HVSPTNRYTT 250
IGTLLVVLIV SAMKECIEDI KRANSDKELN NSTAEIFSEA HDDFVEKRWI 300
DIRVGDIIRV KSEEPIPADT IILSSSEPEG LCYIETANLD GETNLKIKQS 350
RVETAKFIDV KTLKNMNGKV VSEQPNSSLY TYEGTMTLND RQIPLSPDQM 400
ILRGATLRNT AWIFGLVIFT GHETKLLRNA TATPIKRTAV EKIINRQIIA 450
LFTVLIVLIL ISSIGNVIMS TADAKHLSYL YLEGTNKAGL FFKDFLTFWI 500
LFSNLVPISL FVTVELIKYY QAFMIGSDLD LYYEKTDTPT VVRTSSLVEE 550
LGQIEYIFSD KTGTLTRNIM EFKSCSIAGH CYIDKIPEDK TATVEDGIEV 600
GYRKFDDLKK KLNDPSDEDS PIINDFLTLL ATCHTVIPEF QSDGSIKYQA 650
ASPDEGALVQ GGADLGYKFI IRKPNSVTVL LEETGEEKEY QLLNICEFNS 700
TRKRMSAIFR FPDGSIKLFC KGADTVILER LDDEANQYVE ATMRHLEDYA 750
SEGLRTLCLA MRDISEGEYE EWNSIYNEAA TTLDNRAEKL DEAANLIEKN 800
LILIGATAIE DKLQDGVPET IHTLQEAGIK IWVLTGDRQE TAINIGMSCR 850
LLSEDMNLLI INEETRDDTE RNLLEKINAL NEHQLSTHDM NTLALVIDGK 900
SLGFALEPEL EDYLLTVAKL CKAVICCRVS PLQKALVVKM VKRKSSSLLL 950
AIGDGANDVS MIQAAHVGVG ISGMEGMQAA RSADIAVGQF KFLKKLLLVH 1000
GSWSYQRISV AILYSFYKNT ALYMTQFWYV FANAFSGQSI MESWTMSFYN 1050
LFFTVWPPFV IGVFDQFVSS RLLERYPQLY KLGQKGQFFS VYIFWGWIIN 1100
GFFHSAIVFI GTILIYRYGF ALNMHGELAD HWSWGVTVYT TSVIIVLGKA 1150
ALVTNQWTKF TLIAIPGSLL FWLIFFPIYA SIFPHANISR EYYGVVKHTY 1200
GSGVFWLTLI VLPIFALVRD FLWKYYKRMY EPETYHVIQE MQKYNISDSR 1250
PHVQQFQNAI RKVRQVQRMK KQRGFAFSQA EEGGQEKIVR MYDTTQKRGK 1300
YGELQDASAN PFNDNNGLGS NDFESAEPFI ENPFADGNQN SNRFSSSRDD 1350
ISFDI 1355
Length:1,355
Mass (Da):153,765
Last modified:July 27, 2011 - v2
Checksum:i5745B92901F8E1AE
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 462AN → GY in AAA16891. 1 Publication
Sequence conflicti45 – 462AN → GY in AAC05006. 1 Publication
Sequence conflicti450 – 4501A → R in AAA16891. 1 Publication
Sequence conflicti450 – 4501A → R in AAC05006. 1 Publication
Sequence conflicti674 – 6741P → G in AAA16891. 1 Publication
Sequence conflicti674 – 6741P → G in AAC05006. 1 Publication
Sequence conflicti891 – 8922NT → KS in AAA16891. 1 Publication
Sequence conflicti891 – 8922NT → KS in AAC05006. 1 Publication
Sequence conflicti953 – 9542GD → AS in AAA16891. 1 Publication
Sequence conflicti953 – 9542GD → AS in AAC05006. 1 Publication
Sequence conflicti987 – 9871V → L in AAA16891. 1 Publication
Sequence conflicti987 – 9871V → L in AAC05006. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L01795 Unassigned RNA. Translation: AAA16891.1.
U12980 Genomic DNA. Translation: AAC05006.1.
BK006935 Genomic DNA. Translation: DAA06962.2.
PIRiS51995.
RefSeqiNP_009376.2. NM_001178171.2.

Genome annotation databases

EnsemblFungiiYAL026C; YAL026C; YAL026C.
GeneIDi851207.
KEGGisce:YAL026C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L01795 Unassigned RNA. Translation: AAA16891.1 .
U12980 Genomic DNA. Translation: AAC05006.1 .
BK006935 Genomic DNA. Translation: DAA06962.2 .
PIRi S51995.
RefSeqi NP_009376.2. NM_001178171.2.

3D structure databases

ProteinModelPortali P39524.
SMRi P39524. Positions 216-1019.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31740. 189 interactions.
DIPi DIP-2216N.
IntActi P39524. 10 interactions.
MINTi MINT-520976.
STRINGi 4932.YAL026C.

Protein family/group databases

TCDBi 3.A.3.8.2. the p-type atpase (p-atpase) superfamily.

Proteomic databases

MaxQBi P39524.
PaxDbi P39524.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL026C ; YAL026C ; YAL026C .
GeneIDi 851207.
KEGGi sce:YAL026C.

Organism-specific databases

CYGDi YAL026c.
SGDi S000000024. DRS2.

Phylogenomic databases

eggNOGi COG0474.
GeneTreei ENSGT00740000115297.
HOGENOMi HOG000202528.
KOi K14802.
OMAi IPRIYES.
OrthoDBi EOG7FR7QR.

Enzyme and pathway databases

BioCyci YEAST:G3O-28837-MONOMER.
Reactomei REACT_96459. Ion transport by P-type ATPases.

Miscellaneous databases

NextBioi 968076.
PROi P39524.

Gene expression databases

Genevestigatori P39524.

Family and domain databases

Gene3Di 2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProi IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR006539. ATPase_P-typ_Plipid-transp.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
PANTHERi PTHR24092. PTHR24092. 1 hit.
Pfami PF00122. E1-E2_ATPase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SUPFAMi SSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "DRS1 to DRS7, novel genes required for ribosome assembly and function in Saccharomyces cerevisiae."
    Ripmaster T.L., Vaughn G.P., Woolford J.L. Jr.
    Mol. Cell. Biol. 13:7901-7912(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 45-46; 450; 674; 891-892; 953-954 AND 987.
    Strain: ATCC 204508 / S288c.
  4. "Drs2p-dependent formation of exocytic clathrin-coated vesicles in vivo."
    Gall W.E., Geething N.C., Hua Z., Ingram M.F., Liu K., Chen S.I., Graham T.R.
    Curr. Biol. 12:1623-1627(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae."
    Saito K., Fujimura-Kamada K., Furuta N., Kato U., Umeda M., Tanaka K.
    Mol. Biol. Cell 15:3418-3432(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CDC50.
  8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-102, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiATC3_YEAST
AccessioniPrimary (citable) accession number: P39524
Secondary accession number(s): D6VPJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 606 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

Similar proteinsi