P39522 (ILV3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase, mitochondrial
Short name=DAD
EC=4.2.1.9

Alternative name(s):
2,3-dihydroxy acid hydrolyase

Gene names

Name:	ILV3
Ordered Locus Names:	YJR016C
ORF Names:	J1450

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O.
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.
Subunit structure	Homodimer.
Subcellular location	Mitochondrion.
Miscellaneous	Present with 171000 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from mutant phenotype. Source: SGD
Cellular component	mitochondrion Inferred from direct assay. Source: SGD
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from mutant phenotype. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 585		Dihydroxy-acid dehydratase, mitochondrial		PRO_0000015636
Sites
	Metal binding	143	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	221	1	Iron-sulfur (4Fe-4S) Potential
Experimental info
	Sequence conflict	82 – 89	8	SQSIEKAG → MFSIIEKR in AAA34568. Ref.3
	Sequence conflict	238	1	G → S in AAA34568. Ref.3
	Sequence conflict	242	1	P → S in AAA34568. Ref.3
	Sequence conflict	492	1	A → T in AAA34568. Ref.3
	Sequence conflict	520	1	P → R in AAA34568. Ref.3
	Sequence conflict	551	1	R → A in AAA34568. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P39522 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 35AB3C679BA6E8D0	FASTA	585	62,861
	10 20 30 40 50 60 MGLLTKVATS RQFSTTRCVA KKLNKYSYII TEPKGQGASQ AMLYATGFKK EDFKKPQVGV 70 80 90 100 110 120 GSCWWSGNPC NMHLLDLNNR CSQSIEKAGL KAMQFNTIGV SDGISMGTKG MRYSLQSREI 130 140 150 160 170 180 IADSFETIMM AQHYDANIAI PSCDKNMPGV MMAMGRHNRP SIMVYGGTIL PGHPTCGSSK 190 200 210 220 230 240 ISKNIDIVSA FQSYGEYISK QFTEEEREDV VEHACPGPGS CGGMYTANTM ASAAEVLGLT 250 260 270 280 290 300 IPNSSSFPAV SKEKLAECDN IGEYIKKTME LGILPRDILT KEAFENAITY VVATGGSTNA 310 320 330 340 350 360 VLHLVAVAHS AGVKLSPDDF QRISDTTPLI GDFKPSGKYV MADLINVGGT QSVIKYLYEN 370 380 390 400 410 420 NMLHGNTMTV TGDTLAERAK KAPSLPEGQE IIKPLSHPIK ANGHLQILYG SLAPGGAVGK 430 440 450 460 470 480 ITGKEGTYFK GRARVFEEEG AFIEALERGE IKKGEKTVVV IRYEGPRGAP GMPEMLKPSS 490 500 510 520 530 540 ALMGYGLGKD VALLTDGRFS GGSHGFLIGH IVPEAAEGGP IGLVRDGDEI IIDADNNKID 550 560 570 580 LLVSDKEMAQ RKQSWVAPPP RYTRGTLSKY AKLVSNASNG CVLDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Cloning of the dihydroxyacid dehydratase-encoding gene (ILV3) from Saccharomyces cerevisiae." Velasco J.A., Cansado J., Pena M.C., Kawakami T., Laborda J., Notario V. Gene 137:179-185(1993) [PubMed: 8299945] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 82-585. Strain: ATCC 26109 / X2180.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X87611 Genomic DNA. Translation: CAA60939.1. Z49516 Genomic DNA. Translation: CAA89540.1. L13975 Genomic DNA. Translation: AAA34568.1. Different termination. BK006943 Genomic DNA. Translation: DAA08808.1.
PIR	S55205.
RefSeq	NP_012550.1. NM_001181674.1.
3D structure databases
ProteinModelPortal	P39522.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6456N.
IntAct	P39522. 30 interactions.
MINT	MINT-700348.
STRING	P39522.
Proteomic databases
PeptideAtlas	P39522.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR016C; YJR016C; YJR016C.
GeneID	853473.
KEGG	sce:YJR016C.
NMPDR	fig|4932.3.peg.3524.
Organism-specific databases
CYGD	YJR016c.
SGD	S000003777. ILV3.
Phylogenomic databases
eggNOG	fuNOG06287.
GeneTree	EFGT00050000003650.
HOGENOM	HBG671001.
OMA	FENAITY.
OrthoDB	EOG4VDT6Q.
Gene expression databases
ArrayExpress	P39522.
Genevestigator	P39522.
GermOnline	YJR016C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974077.

Entry information

Entry name

ILV3_YEAST

Accession

Primary (citable) accession number: P39522
Secondary accession number(s): D6VWJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 104 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program