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P39521

- FHL1_YEAST

UniProt

P39521 - FHL1_YEAST

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Protein

Pre-rRNA-processing protein FHL1

Gene

FHL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Controls the pre-rRNA processing machinery in conjunction with IFH1. Presumably acts as a transcriptional regulator of genes specifically involved in that process. IFH1 convert FHL1 from a repressor to an activator.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi460 – 55293Fork-headPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. RNA polymerase II transcription coactivator binding Source: SGD
  2. RNA polymerase II transcription corepressor binding Source: SGD
  3. sequence-specific DNA binding Source: InterPro
  4. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter Source: SGD
  2. positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter Source: SGD
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34244-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-rRNA-processing protein FHL1
Gene namesi
Name:FHL1
Ordered Locus Names:YPR104C
ORF Names:P8283.15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XVI

Organism-specific databases

CYGDiYPR104c.
SGDiS000006308. FHL1.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nuclear chromatin Source: SGD
  2. nucleolus Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 936936Pre-rRNA-processing protein FHL1PRO_0000091901Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei44 – 441Phosphoserine2 Publications
Modified residuei228 – 2281Phosphoserine1 Publication
Modified residuei230 – 2301Phosphothreonine1 Publication
Modified residuei247 – 2471Phosphothreonine1 Publication
Modified residuei264 – 2641Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP39521.
PaxDbiP39521.
PeptideAtlasiP39521.

Expressioni

Gene expression databases

GenevestigatoriP39521.

Interactioni

Protein-protein interaction databases

BioGridi36270. 42 interactions.
DIPiDIP-3821N.
IntActiP39521. 1 interaction.
MINTiMINT-495767.
STRINGi4932.YPR104C.

Structurei

3D structure databases

ProteinModelPortaliP39521.
SMRiP39521. Positions 461-534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini300 – 35758FHAPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi816 – 86247Ser-richAdd
BLAST

Sequence similaritiesi

Contains 1 FHA domain.PROSITE-ProRule annotation
Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5025.
GeneTreeiENSGT00760000118794.
HOGENOMiHOG000000999.
InParanoidiP39521.
OrthoDBiEOG7J70RS.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.20. 1 hit.
InterProiIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
IPR001766. TF_fork_head.
IPR018122. TF_fork_head_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
PF00250. Fork_head. 1 hit.
[Graphical view]
PRINTSiPR00053. FORKHEAD.
SMARTiSM00339. FH. 1 hit.
SM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39521-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDGEMAIIES SNHVGTSSPT TETQFTIDSS ALKDQETKES ITNSPTSEVP
60 70 80 90 100
IETKLPKSSD IVTEEKHPQN TTTDIENEVE NPVTDDNGNL KLELPDNLDN
110 120 130 140 150
ADFSKLLEFD AKNDEALFNS NELLSHTMDP VNNIDLTHDH SREVSSKEDI
160 170 180 190 200
NIEPVNPDED EREKTQDNTA AVKTEGIRNS EDTSIQKDEP TADAIYTDVH
210 220 230 240 250
KLSVNKDTET LPTLVDEKNN MLHMRNNSIT PIMFQQHELV GQPPQNTVTE
260 270 280 290 300
NNSTDAETTQ RKLSEPIDAS LPLPNEQPTI FAYARLDFQS FTFYVQTLHA
310 320 330 340 350
IIGRRSENDF SHKVDVNLGP SKSISRRHAQ IFYNFGTGRF ELSIIGKNGA
360 370 380 390 400
FVDDIFVEKG NTVPLRNKTK IQIGQIPFQF ILPEQERNDD SKSPENADIA
410 420 430 440 450
ESEINTRNLK KNEPKSKKKI TTGAKPKKAQ TKPAVKKEKK PPKIPKKVYT
460 470 480 490 500
LEEIPVEYRT KPTVSYSAML TTCIRKYSTA KGMSLSEIYA GIRELFPYYK
510 520 530 540 550
YCPDGWQSSV RHNLSLNKSF RKVSKEGKGW LWGLDEEYIA ERERQKKKQS
560 570 580 590 600
EIAVAKAQAA QLKLEQQQHK LQQVPQRGKK DIVSQRSNVN ARKQNISQTL
610 620 630 640 650
AANRAASNRK NTASDNQRTM KYLQEQLVIL TRDRKGLSKQ VIAAILTQAL
660 670 680 690 700
AMTINQVTQA AKNKGITGNP LTALMDKNPQ HLNLILAAAV NAATAKVTKG
710 720 730 740 750
EVKQLVNPET TAAAALAAKA QHSKPIRQPI VQTPHVPDRP PSQLSASASS
760 770 780 790 800
HPNNYLHDKQ PGSFDPSSLS RFFQPRQNAR ATSSVAATSV PAAASQNVDA
810 820 830 840 850
QPKPKPAQDN DLESESGTSS SSSSSSESGS ESDSGSDDGS ASGSGDNSST
860 870 880 890 900
SSESESESDS GSEVDEKNNK NEKIDSESIK NNESKDDIPS KDENSSNDNR
910 920 930
EISKTDEEGH DSKRRKVSED INEGITEVNV SLEEKL
Length:936
Mass (Da):103,502
Last modified:February 1, 1995 - v1
Checksum:i2FC02DFC0DFFF503
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z28348 Genomic DNA. Translation: CAA82202.1.
U32445 Genomic DNA. Translation: AAB68074.1.
BK006949 Genomic DNA. Translation: DAA11518.1.
PIRiS43738.
RefSeqiNP_015429.1. NM_001184201.1.

Genome annotation databases

EnsemblFungiiYPR104C; YPR104C; YPR104C.
GeneIDi856219.
KEGGisce:YPR104C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z28348 Genomic DNA. Translation: CAA82202.1 .
U32445 Genomic DNA. Translation: AAB68074.1 .
BK006949 Genomic DNA. Translation: DAA11518.1 .
PIRi S43738.
RefSeqi NP_015429.1. NM_001184201.1.

3D structure databases

ProteinModelPortali P39521.
SMRi P39521. Positions 461-534.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 36270. 42 interactions.
DIPi DIP-3821N.
IntActi P39521. 1 interaction.
MINTi MINT-495767.
STRINGi 4932.YPR104C.

Proteomic databases

MaxQBi P39521.
PaxDbi P39521.
PeptideAtlasi P39521.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YPR104C ; YPR104C ; YPR104C .
GeneIDi 856219.
KEGGi sce:YPR104C.

Organism-specific databases

CYGDi YPR104c.
SGDi S000006308. FHL1.

Phylogenomic databases

eggNOGi COG5025.
GeneTreei ENSGT00760000118794.
HOGENOMi HOG000000999.
InParanoidi P39521.
OrthoDBi EOG7J70RS.

Enzyme and pathway databases

BioCyci YEAST:G3O-34244-MONOMER.

Miscellaneous databases

NextBioi 981444.

Gene expression databases

Genevestigatori P39521.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
2.60.200.20. 1 hit.
InterProi IPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
IPR001766. TF_fork_head.
IPR018122. TF_fork_head_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00498. FHA. 1 hit.
PF00250. Fork_head. 1 hit.
[Graphical view ]
PRINTSi PR00053. FORKHEAD.
SMARTi SM00339. FH. 1 hit.
SM00240. FHA. 1 hit.
[Graphical view ]
SUPFAMi SSF49879. SSF49879. 1 hit.
PROSITEi PS50006. FHA_DOMAIN. 1 hit.
PS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Suppression of yeast RNA polymerase III mutations by FHL1, a gene coding for a fork head protein involved in rRNA processing."
    Hermann-Ledenmat S., Werner M., Sentenac A., Thuriaux P.
    Mol. Cell. Biol. 14:2905-2913(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-228; THR-230; THR-247 AND SER-264, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFHL1_YEAST
AccessioniPrimary (citable) accession number: P39521
Secondary accession number(s): D6W4A2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 639 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3