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P39521 (FHL1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-rRNA-processing protein FHL1
Gene names
Name:FHL1
Ordered Locus Names:YPR104C
ORF Names:P8283.15
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length936 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Controls the pre-rRNA processing machinery in conjunction with IFH1. Presumably acts as a transcriptional regulator of genes specifically involved in that process. IFH1 convert FHL1 from a repressor to an activator.

Subcellular location

Nucleus Probable.

Miscellaneous

Present with 639 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 FHA domain.

Contains 1 fork-head DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 936936Pre-rRNA-processing protein FHL1
PRO_0000091901

Regions

Domain300 – 35758FHA
DNA binding460 – 55293Fork-head
Compositional bias816 – 86247Ser-rich

Amino acid modifications

Modified residue441Phosphoserine Ref.5 Ref.6
Modified residue2281Phosphoserine Ref.8
Modified residue2301Phosphothreonine Ref.8
Modified residue2471Phosphothreonine Ref.8
Modified residue2641Phosphoserine Ref.7 Ref.8

Sequences

Sequence LengthMass (Da)Tools
P39521 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 2FC02DFC0DFFF503

FASTA936103,502
        10         20         30         40         50         60 
MDGEMAIIES SNHVGTSSPT TETQFTIDSS ALKDQETKES ITNSPTSEVP IETKLPKSSD 

        70         80         90        100        110        120 
IVTEEKHPQN TTTDIENEVE NPVTDDNGNL KLELPDNLDN ADFSKLLEFD AKNDEALFNS 

       130        140        150        160        170        180 
NELLSHTMDP VNNIDLTHDH SREVSSKEDI NIEPVNPDED EREKTQDNTA AVKTEGIRNS 

       190        200        210        220        230        240 
EDTSIQKDEP TADAIYTDVH KLSVNKDTET LPTLVDEKNN MLHMRNNSIT PIMFQQHELV 

       250        260        270        280        290        300 
GQPPQNTVTE NNSTDAETTQ RKLSEPIDAS LPLPNEQPTI FAYARLDFQS FTFYVQTLHA 

       310        320        330        340        350        360 
IIGRRSENDF SHKVDVNLGP SKSISRRHAQ IFYNFGTGRF ELSIIGKNGA FVDDIFVEKG 

       370        380        390        400        410        420 
NTVPLRNKTK IQIGQIPFQF ILPEQERNDD SKSPENADIA ESEINTRNLK KNEPKSKKKI 

       430        440        450        460        470        480 
TTGAKPKKAQ TKPAVKKEKK PPKIPKKVYT LEEIPVEYRT KPTVSYSAML TTCIRKYSTA 

       490        500        510        520        530        540 
KGMSLSEIYA GIRELFPYYK YCPDGWQSSV RHNLSLNKSF RKVSKEGKGW LWGLDEEYIA 

       550        560        570        580        590        600 
ERERQKKKQS EIAVAKAQAA QLKLEQQQHK LQQVPQRGKK DIVSQRSNVN ARKQNISQTL 

       610        620        630        640        650        660 
AANRAASNRK NTASDNQRTM KYLQEQLVIL TRDRKGLSKQ VIAAILTQAL AMTINQVTQA 

       670        680        690        700        710        720 
AKNKGITGNP LTALMDKNPQ HLNLILAAAV NAATAKVTKG EVKQLVNPET TAAAALAAKA 

       730        740        750        760        770        780 
QHSKPIRQPI VQTPHVPDRP PSQLSASASS HPNNYLHDKQ PGSFDPSSLS RFFQPRQNAR 

       790        800        810        820        830        840 
ATSSVAATSV PAAASQNVDA QPKPKPAQDN DLESESGTSS SSSSSSESGS ESDSGSDDGS 

       850        860        870        880        890        900 
ASGSGDNSST SSESESESDS GSEVDEKNNK NEKIDSESIK NNESKDDIPS KDENSSNDNR 

       910        920        930 
EISKTDEEGH DSKRRKVSED INEGITEVNV SLEEKL 

« Hide

References

« Hide 'large scale' references
[1]"Suppression of yeast RNA polymerase III mutations by FHL1, a gene coding for a fork head protein involved in rRNA processing."
Hermann-Ledenmat S., Werner M., Sentenac A., Thuriaux P.
Mol. Cell. Biol. 14:2905-2913(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-228; THR-230; THR-247 AND SER-264, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z28348 Genomic DNA. Translation: CAA82202.1.
U32445 Genomic DNA. Translation: AAB68074.1.
BK006949 Genomic DNA. Translation: DAA11518.1.
PIRS43738.
RefSeqNP_015429.1. NM_001184201.1.

3D structure databases

ProteinModelPortalP39521.
SMRP39521. Positions 292-385, 461-534.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36270. 42 interactions.
DIPDIP-3821N.
IntActP39521. 1 interaction.
MINTMINT-495767.
STRING4932.YPR104C.

Proteomic databases

MaxQBP39521.
PaxDbP39521.
PeptideAtlasP39521.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR104C; YPR104C; YPR104C.
GeneID856219.
KEGGsce:YPR104C.

Organism-specific databases

CYGDYPR104c.
SGDS000006308. FHL1.

Phylogenomic databases

eggNOGCOG5025.
GeneTreeENSGT00750000117242.
HOGENOMHOG000000999.
OrthoDBEOG7J70RS.

Enzyme and pathway databases

BioCycYEAST:G3O-34244-MONOMER.

Gene expression databases

GenevestigatorP39521.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
2.60.200.20. 1 hit.
InterProIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
IPR001766. TF_fork_head.
IPR018122. TF_fork_head_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00498. FHA. 1 hit.
PF00250. Fork_head. 1 hit.
[Graphical view]
PRINTSPR00053. FORKHEAD.
SMARTSM00339. FH. 1 hit.
SM00240. FHA. 1 hit.
[Graphical view]
SUPFAMSSF49879. SSF49879. 1 hit.
PROSITEPS50006. FHA_DOMAIN. 1 hit.
PS00657. FORK_HEAD_1. 1 hit.
PS00658. FORK_HEAD_2. 1 hit.
PS50039. FORK_HEAD_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio981444.

Entry information

Entry nameFHL1_YEAST
AccessionPrimary (citable) accession number: P39521
Secondary accession number(s): D6W4A2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families