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P39521

- FHL1_YEAST

UniProt

P39521 - FHL1_YEAST

Protein

Pre-rRNA-processing protein FHL1

Gene

FHL1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Controls the pre-rRNA processing machinery in conjunction with IFH1. Presumably acts as a transcriptional regulator of genes specifically involved in that process. IFH1 convert FHL1 from a repressor to an activator.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi460 – 55293Fork-headPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA polymerase II transcription coactivator binding Source: SGD
    2. RNA polymerase II transcription corepressor binding Source: SGD
    3. sequence-specific DNA binding Source: InterPro
    4. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter Source: SGD
    2. positive regulation of ribosomal protein gene transcription from RNA polymerase II promoter Source: SGD
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34244-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-rRNA-processing protein FHL1
    Gene namesi
    Name:FHL1
    Ordered Locus Names:YPR104C
    ORF Names:P8283.15
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR104c.
    SGDiS000006308. FHL1.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nuclear chromatin Source: SGD
    2. nucleolus Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 936936Pre-rRNA-processing protein FHL1PRO_0000091901Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei44 – 441Phosphoserine2 Publications
    Modified residuei228 – 2281Phosphoserine1 Publication
    Modified residuei230 – 2301Phosphothreonine1 Publication
    Modified residuei247 – 2471Phosphothreonine1 Publication
    Modified residuei264 – 2641Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP39521.
    PaxDbiP39521.
    PeptideAtlasiP39521.

    Expressioni

    Gene expression databases

    GenevestigatoriP39521.

    Interactioni

    Protein-protein interaction databases

    BioGridi36270. 42 interactions.
    DIPiDIP-3821N.
    IntActiP39521. 1 interaction.
    MINTiMINT-495767.
    STRINGi4932.YPR104C.

    Structurei

    3D structure databases

    ProteinModelPortaliP39521.
    SMRiP39521. Positions 292-385, 461-534.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini300 – 35758FHAPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi816 – 86247Ser-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FHA domain.PROSITE-ProRule annotation
    Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5025.
    GeneTreeiENSGT00750000117242.
    HOGENOMiHOG000000999.
    OrthoDBiEOG7J70RS.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.200.20. 1 hit.
    InterProiIPR000253. FHA_dom.
    IPR008984. SMAD_FHA_domain.
    IPR001766. TF_fork_head.
    IPR018122. TF_fork_head_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00498. FHA. 1 hit.
    PF00250. Fork_head. 1 hit.
    [Graphical view]
    PRINTSiPR00053. FORKHEAD.
    SMARTiSM00339. FH. 1 hit.
    SM00240. FHA. 1 hit.
    [Graphical view]
    SUPFAMiSSF49879. SSF49879. 1 hit.
    PROSITEiPS50006. FHA_DOMAIN. 1 hit.
    PS00657. FORK_HEAD_1. 1 hit.
    PS00658. FORK_HEAD_2. 1 hit.
    PS50039. FORK_HEAD_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P39521-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDGEMAIIES SNHVGTSSPT TETQFTIDSS ALKDQETKES ITNSPTSEVP    50
    IETKLPKSSD IVTEEKHPQN TTTDIENEVE NPVTDDNGNL KLELPDNLDN 100
    ADFSKLLEFD AKNDEALFNS NELLSHTMDP VNNIDLTHDH SREVSSKEDI 150
    NIEPVNPDED EREKTQDNTA AVKTEGIRNS EDTSIQKDEP TADAIYTDVH 200
    KLSVNKDTET LPTLVDEKNN MLHMRNNSIT PIMFQQHELV GQPPQNTVTE 250
    NNSTDAETTQ RKLSEPIDAS LPLPNEQPTI FAYARLDFQS FTFYVQTLHA 300
    IIGRRSENDF SHKVDVNLGP SKSISRRHAQ IFYNFGTGRF ELSIIGKNGA 350
    FVDDIFVEKG NTVPLRNKTK IQIGQIPFQF ILPEQERNDD SKSPENADIA 400
    ESEINTRNLK KNEPKSKKKI TTGAKPKKAQ TKPAVKKEKK PPKIPKKVYT 450
    LEEIPVEYRT KPTVSYSAML TTCIRKYSTA KGMSLSEIYA GIRELFPYYK 500
    YCPDGWQSSV RHNLSLNKSF RKVSKEGKGW LWGLDEEYIA ERERQKKKQS 550
    EIAVAKAQAA QLKLEQQQHK LQQVPQRGKK DIVSQRSNVN ARKQNISQTL 600
    AANRAASNRK NTASDNQRTM KYLQEQLVIL TRDRKGLSKQ VIAAILTQAL 650
    AMTINQVTQA AKNKGITGNP LTALMDKNPQ HLNLILAAAV NAATAKVTKG 700
    EVKQLVNPET TAAAALAAKA QHSKPIRQPI VQTPHVPDRP PSQLSASASS 750
    HPNNYLHDKQ PGSFDPSSLS RFFQPRQNAR ATSSVAATSV PAAASQNVDA 800
    QPKPKPAQDN DLESESGTSS SSSSSSESGS ESDSGSDDGS ASGSGDNSST 850
    SSESESESDS GSEVDEKNNK NEKIDSESIK NNESKDDIPS KDENSSNDNR 900
    EISKTDEEGH DSKRRKVSED INEGITEVNV SLEEKL 936
    Length:936
    Mass (Da):103,502
    Last modified:February 1, 1995 - v1
    Checksum:i2FC02DFC0DFFF503
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28348 Genomic DNA. Translation: CAA82202.1.
    U32445 Genomic DNA. Translation: AAB68074.1.
    BK006949 Genomic DNA. Translation: DAA11518.1.
    PIRiS43738.
    RefSeqiNP_015429.1. NM_001184201.1.

    Genome annotation databases

    EnsemblFungiiYPR104C; YPR104C; YPR104C.
    GeneIDi856219.
    KEGGisce:YPR104C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28348 Genomic DNA. Translation: CAA82202.1 .
    U32445 Genomic DNA. Translation: AAB68074.1 .
    BK006949 Genomic DNA. Translation: DAA11518.1 .
    PIRi S43738.
    RefSeqi NP_015429.1. NM_001184201.1.

    3D structure databases

    ProteinModelPortali P39521.
    SMRi P39521. Positions 292-385, 461-534.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36270. 42 interactions.
    DIPi DIP-3821N.
    IntActi P39521. 1 interaction.
    MINTi MINT-495767.
    STRINGi 4932.YPR104C.

    Proteomic databases

    MaxQBi P39521.
    PaxDbi P39521.
    PeptideAtlasi P39521.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR104C ; YPR104C ; YPR104C .
    GeneIDi 856219.
    KEGGi sce:YPR104C.

    Organism-specific databases

    CYGDi YPR104c.
    SGDi S000006308. FHL1.

    Phylogenomic databases

    eggNOGi COG5025.
    GeneTreei ENSGT00750000117242.
    HOGENOMi HOG000000999.
    OrthoDBi EOG7J70RS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34244-MONOMER.

    Miscellaneous databases

    NextBioi 981444.

    Gene expression databases

    Genevestigatori P39521.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.200.20. 1 hit.
    InterProi IPR000253. FHA_dom.
    IPR008984. SMAD_FHA_domain.
    IPR001766. TF_fork_head.
    IPR018122. TF_fork_head_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00498. FHA. 1 hit.
    PF00250. Fork_head. 1 hit.
    [Graphical view ]
    PRINTSi PR00053. FORKHEAD.
    SMARTi SM00339. FH. 1 hit.
    SM00240. FHA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49879. SSF49879. 1 hit.
    PROSITEi PS50006. FHA_DOMAIN. 1 hit.
    PS00657. FORK_HEAD_1. 1 hit.
    PS00658. FORK_HEAD_2. 1 hit.
    PS50039. FORK_HEAD_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Suppression of yeast RNA polymerase III mutations by FHL1, a gene coding for a fork head protein involved in rRNA processing."
      Hermann-Ledenmat S., Werner M., Sentenac A., Thuriaux P.
      Mol. Cell. Biol. 14:2905-2913(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-264, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-228; THR-230; THR-247 AND SER-264, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiFHL1_YEAST
    AccessioniPrimary (citable) accession number: P39521
    Secondary accession number(s): D6W4A2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 138 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 639 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3