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Protein

Long-chain-fatty-acid--CoA ligase 2

Gene

FAA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Esterification, concomitant with transport, of endogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. Preferentially acts on C9:0-C13:0 fatty acids although C7:0-C17:0 fatty acids are tolerated.

Miscellaneous

The FAM1-1 suppressor mutant has a single base mutation that creates a new initiator methionine and adds 18 residues at the N-terminus of the protein. This extra sequence acts as a transit peptide and the resulting protein is imported in the mitochondria.
Present with 358 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity

Temperature dependencei

Optimum temperature is 25 degrees Celsius.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • decanoate-CoA ligase activity Source: UniProtKB-EC
  • long-chain fatty acid-CoA ligase activity Source: SGD
  • medium-chain fatty acid-CoA ligase activity Source: SGD
  • very long-chain fatty acid-CoA ligase activity Source: SGD

GO - Biological processi

  • fatty acid beta-oxidation Source: UniProtKB
  • fatty-acyl-CoA transport Source: UniProtKB
  • long-chain fatty acid metabolic process Source: SGD
  • peroxisomal long-chain fatty acid import Source: UniProtKB
  • very long-chain fatty acid catabolic process Source: UniProtKB

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YER015W-MONOMER
YEAST:YER015W-MONOMER
BRENDAi6.2.1.3 984
ReactomeiR-SCE-2046105 Linoleic acid (LA) metabolism
R-SCE-2046106 alpha-linolenic acid (ALA) metabolism
R-SCE-75876 Synthesis of very long-chain fatty acyl-CoAs

Protein family/group databases

TCDBi4.C.1.1.15 the fatty acid transporter (fat) family

Chemistry databases

SwissLipidsiSLP:000001032

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 2 (EC:6.2.1.3)
Alternative name(s):
Fatty acid activator 2
Long-chain acyl-CoA synthetase 2
Gene namesi
Name:FAA2
Synonyms:FAM1
Ordered Locus Names:YER015W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YER015W
SGDiS000000817 FAA2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1M → MWKNAGYKKRIRTNLFRNM in FAM1-1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931201 – 744Long-chain-fatty-acid--CoA ligase 2Add BLAST744

Proteomic databases

MaxQBiP39518
PaxDbiP39518
PRIDEiP39518

Interactioni

Protein-protein interaction databases

BioGridi36747, 17 interactors
DIPiDIP-6565N
IntActiP39518, 5 interactors
MINTiP39518
STRINGi4932.YER015W

Structurei

3D structure databases

ProteinModelPortaliP39518
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00690000101725
HOGENOMiHOG000159459
InParanoidiP39518
KOiK01897
OMAiECVMLCH
OrthoDBiEOG092C0RYI

Family and domain databases

InterProiView protein in InterPro
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

P39518-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPDYALTD LIESDPRFES LKTRLAGYTK GSDEYIEELY SQLPLTSYPR
60 70 80 90 100
YKTFLKKQAV AISNPDNEAG FSSIYRSSLS SENLVSCVDK NLRTAYDHFM
110 120 130 140 150
FSARRWPQRD CLGSRPIDKA TGTWEETFRF ESYSTVSKRC HNIGSGILSL
160 170 180 190 200
VNTKRKRPLE ANDFVVAILS HNNPEWILTD LACQAYSLTN TALYETLGPN
210 220 230 240 250
TSEYILNLTE APILIFAKSN MYHVLKMVPD MKFVNTLVCM DELTHDELRM
260 270 280 290 300
LNESLLPVKC NSLNEKITFF SLEQVEQVGC FNKIPAIPPT PDSLYTISFT
310 320 330 340 350
SGTTGLPKGV EMSHRNIASG IAFAFSTFRI PPDKRNQQLY DMCFLPLAHI
360 370 380 390 400
FERMVIAYDL AIGFGIGFLH KPDPTVLVED LKILKPYAVA LVPRILTRFE
410 420 430 440 450
AGIKNALDKS TVQRNVANTI LDSKSARFTA RGGPDKSIMN FLVYHRVLID
460 470 480 490 500
KIRDSLGLSN NSFIITGSAP ISKDTLLFLR SALDIGIRQG YGLTETFAGV
510 520 530 540 550
CLSEPFEKDV GSCGAIGISA ECRLKSVPEM GYHADKDLKG ELQIRGPQVF
560 570 580 590 600
ERYFKNPNET SKAVDQDGWF STGDVAFIDG KGRISVIDRV KNFFKLAHGE
610 620 630 640 650
YIAPEKIENI YLSSCPYITQ IFVFGDPLKT FLVGIVGVDV DAAQPILAAK
660 670 680 690 700
HPEVKTWTKE VLVENLNRNK KLRKEFLNKI NKCTDGLQGF EKLHNIKVGL
710 720 730 740
EPLTLEDDVV TPTFKIKRAK ASKFFKDTLD QLYAEGSLVK TEKL
Length:744
Mass (Da):83,438
Last modified:February 1, 1995 - v1
Checksum:i1E023C18682805BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77783 Genomic DNA Translation: CAA54817.1
X82364 Genomic DNA Translation: CAA57780.1
U18778 Genomic DNA Translation: AAB64548.1
BK006939 Genomic DNA Translation: DAA07667.1
PIRiA54901
RefSeqiNP_010931.3, NM_001178906.3

Genome annotation databases

EnsemblFungiiYER015W; YER015W; YER015W
GeneIDi856734
KEGGisce:YER015W

Similar proteinsi

Entry informationi

Entry nameiLCF2_YEAST
AccessioniPrimary (citable) accession number: P39518
Secondary accession number(s): D3DLR3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

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