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P39518 (LCF2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Long-chain-fatty-acid--CoA ligase 2

EC=6.2.1.3
Alternative name(s):
Fatty acid activator 2
Long-chain acyl-CoA synthetase 2
Gene names
Name:FAA2
Synonyms:FAM1
Ordered Locus Names:YER015W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length744 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Esterification, concomitant with transport, of endogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. Preferentially acts on C9:0-C13:0 fatty acids although C7:0-C17:0 fatty acids are tolerated.

Catalytic activity

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactor

Magnesium By similarity.

Subcellular location

Cytoplasm. Mitochondrion. Note: The FAM1-1 mutant is imported in the mitochondria.

Miscellaneous

The FAM1-1 suppressor mutant has a single base mutation that creates a new initiator methionine and adds 18 residues at the N-terminus of the protein. This extra sequence acts as a transit peptide and the resulting protein is imported in the mitochondria.

Present with 358 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

Biophysicochemical properties

Temperature dependence:

Optimum temperature is 25 degrees Celsius.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 744744Long-chain-fatty-acid--CoA ligase 2
PRO_0000193120

Experimental info

Mutagenesis11M → MWKNAGYKKRIRTNLFRNM in FAM1-1. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P39518 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 1E023C18682805BC

FASTA74483,438
        10         20         30         40         50         60 
MAAPDYALTD LIESDPRFES LKTRLAGYTK GSDEYIEELY SQLPLTSYPR YKTFLKKQAV 

        70         80         90        100        110        120 
AISNPDNEAG FSSIYRSSLS SENLVSCVDK NLRTAYDHFM FSARRWPQRD CLGSRPIDKA 

       130        140        150        160        170        180 
TGTWEETFRF ESYSTVSKRC HNIGSGILSL VNTKRKRPLE ANDFVVAILS HNNPEWILTD 

       190        200        210        220        230        240 
LACQAYSLTN TALYETLGPN TSEYILNLTE APILIFAKSN MYHVLKMVPD MKFVNTLVCM 

       250        260        270        280        290        300 
DELTHDELRM LNESLLPVKC NSLNEKITFF SLEQVEQVGC FNKIPAIPPT PDSLYTISFT 

       310        320        330        340        350        360 
SGTTGLPKGV EMSHRNIASG IAFAFSTFRI PPDKRNQQLY DMCFLPLAHI FERMVIAYDL 

       370        380        390        400        410        420 
AIGFGIGFLH KPDPTVLVED LKILKPYAVA LVPRILTRFE AGIKNALDKS TVQRNVANTI 

       430        440        450        460        470        480 
LDSKSARFTA RGGPDKSIMN FLVYHRVLID KIRDSLGLSN NSFIITGSAP ISKDTLLFLR 

       490        500        510        520        530        540 
SALDIGIRQG YGLTETFAGV CLSEPFEKDV GSCGAIGISA ECRLKSVPEM GYHADKDLKG 

       550        560        570        580        590        600 
ELQIRGPQVF ERYFKNPNET SKAVDQDGWF STGDVAFIDG KGRISVIDRV KNFFKLAHGE 

       610        620        630        640        650        660 
YIAPEKIENI YLSSCPYITQ IFVFGDPLKT FLVGIVGVDV DAAQPILAAK HPEVKTWTKE 

       670        680        690        700        710        720 
VLVENLNRNK KLRKEFLNKI NKCTDGLQGF EKLHNIKVGL EPLTLEDDVV TPTFKIKRAK 

       730        740 
ASKFFKDTLD QLYAEGSLVK TEKL 

« Hide

References

« Hide 'large scale' references
[1]"Genetic analysis of the role of Saccharomyces cerevisiae acyl-CoA synthetase genes in regulating protein N-myristoylation."
Johnson D.R., Knoll L.J., Rowley N., Gordon J.I.
J. Biol. Chem. 269:18037-18046(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"Subcellular relocalization of a long-chain fatty acid CoA ligase by a suppressor mutation alleviates a respiration deficiency in Saccharomyces cerevisiae."
Harington A., Schwarz E., Slonimski P.P., Herbert C.J.
EMBO J. 13:5531-5538(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF MET-1.
Strain: CW04.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome V."
Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E., Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S., Hyman R.W. expand/collapse author list , Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X., Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y., Botstein D., Davis R.W.
Nature 387:78-81(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77783 Genomic DNA. Translation: CAA54817.1.
X82364 Genomic DNA. Translation: CAA57780.1.
U18778 Genomic DNA. Translation: AAB64548.1.
BK006939 Genomic DNA. Translation: DAA07667.1.
PIRA54901.
RefSeqNP_010931.3. NM_001178906.3.

3D structure databases

ProteinModelPortalP39518.
SMRP39518. Positions 162-682.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36747. 9 interactions.
DIPDIP-6565N.
IntActP39518. 4 interactions.
MINTMINT-676865.
STRING4932.YER015W.

Protein family/group databases

TCDB4.C.1.1.15. the proposed fatty acid transporter (fat) family.

Proteomic databases

PaxDbP39518.
PeptideAtlasP39518.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYER015W; YER015W; YER015W.
GeneID856734.
KEGGsce:YER015W.

Organism-specific databases

CYGDYER015w.
SGDS000000817. FAA2.

Phylogenomic databases

eggNOGCOG1022.
GeneTreeENSGT00690000101725.
HOGENOMHOG000159459.
KOK01897.
OMAPERIENQ.
OrthoDBEOG7TTQH5.

Enzyme and pathway databases

BioCycMetaCyc:YER015W-MONOMER.
YEAST:YER015W-MONOMER.
BRENDA6.2.1.3. 984.

Gene expression databases

GenevestigatorP39518.

Family and domain databases

InterProIPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio982852.

Entry information

Entry nameLCF2_YEAST
AccessionPrimary (citable) accession number: P39518
Secondary accession number(s): D3DLR3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome V

Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families