Reviewed, UniProtKB/Swiss-Prot P39516 (RS14B_YEAST)

Names and origin

Protein names

Recommended name:
    40S ribosomal protein S14-B
Alternative name(s):
    RP59B

Gene names

Name:	RPS14B
Synonyms:	CRY2
Ordered Locus Names:	YJL191W
ORF Names:	J0354

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. Ref.7
Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Interacts with snoRNA U3. Interacts with MPP10. Component of the ribosomal small subunit (SSU) processome composed of at least 40 protein subunits and snoRNA U3. Ref.7 Ref.4
Subcellular location	Cytoplasm. Nucleus › nucleolus Ref.7 Ref.5.
Miscellaneous	Present with 3370 molecules/cell in log phase SD medium. Ref.6 There are 2 genes for S14 in yeast.
Sequence similarities	Belongs to the ribosomal protein S11P family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm Nucleus
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from mutant phenotype. Source: SGD ribosomal small subunit assembly Inferred from mutant phenotype. Source: UniProtKB translation Ref.4 Traceable author statement. Source: UniProtKB
Cellular component	cytosolic small ribosomal subunit Ref.4 Traceable author statement. Source: UniProtKB nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell small-subunit processome Ref.7 Inferred from direct assay. Source: SGD
Molecular function	SSU rRNA binding Inferred from direct assay. Source: SGD structural constituent of ribosome Ref.4 Traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 138	137	40S ribosomal protein S14-B		PRO_0000123360
Experimental info
	Sequence conflict	3	1	N → K in CAA54769. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P39516-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 6BBACF61D38538AA	FASTA	138	14,650
	10 20 30 40 50 60 MANDLVQARD NSQVFGVARI YASFNDTFVH VTDLSGKETI ARVTGGMKVK ADRDESSPYA 70 80 90 100 110 120 AMLAAQDVAA KCKEVGITAV HVKIRATGGT RTKTPGPGGQ AALRALARSG LRIGRIEDVT 130 PVPSDSTRKK GGRRGRRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular genetics of cryptopleurine resistance in Saccharomyces cerevisiae: expression of a ribosomal protein gene family." Paulovich A.G., Thompson J.R., Larkin J.C., Li Z., Woolford J.L. Jr. Genetics 135:719-730(1993) [PubMed: 8293976] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of a 36 kb segment on the left arm of yeast chromosome X identifies 24 open reading frames including NUC1, PRP21 (SPP91), CDC6, CRY2, the gene for S24, a homologue to the aconitase gene ACO1 and two homologues to chromosome III genes." Purnelle B., Coster F., Goffeau A. Yeast 10:1235-1249(1994) [PubMed: 7754713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed: 9559554] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"The small-subunit processome is a ribosome assembly intermediate." Bernstein K.A., Gallagher J.E.G., Mitchell B.M., Granneman S., Baserga S.J. Eukaryot. Cell 3:1619-1626(2004) [PubMed: 15590835] [Abstract] Cited for: FUNCTION, INTERACTION WITH MPP10 AND SNORNA U3, IDENTIFICATION IN SSU PROCESSOME, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L12564 Genomic DNA. Translation: AAA17764.1. X77688 Genomic DNA. Translation: CAA54769.1. Z49466 Genomic DNA. Translation: CAA89486.1.
PIR	S46643.
RefSeq	NP_012344.1.
3D structure databases
SMR	P39516. Positions 10-134.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5688N.
IntAct	P39516. 34 interactions.
STRING	P39516.
Proteomic databases
PRIDE	P39516.
Genome annotation databases
Ensembl	YJL191W; YJL191W; YJL191W; Saccharomyces cerevisiae. [Genome view]
GeneID	853248.
KEGG	sce:YJL191W.
NMPDR	fig|4932.3.peg.3308.
Organism-specific databases
CYGD	YJL191w.
SGD	S000003727. RPS14B.
Phylogenomic databases
HOGENOM	HBG385416.
OMA	QIESMEL.
OrthoDB	EOG90CK0G.
Gene expression databases
ArrayExpress	P39516.
Genevestigator	P39516.
GermOnline	YJL191W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001971. Ribosomal_S11. IPR018102. Ribosomal_S11_CS. [Graphical view]
Gene3D	G3DSA:3.30.420.80. Ribosomal_S11. 1 hit.
PANTHER	PTHR11759. Ribosomal_S11. 1 hit.
Pfam	PF00411. Ribosomal_S11. 1 hit. [Graphical view]
PIRSF	PIRSF002131. Ribosomal_S11. 1 hit.
PROSITE	PS00054. RIBOSOMAL_S11. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	973487.

Entry information

Entry name

RS14B_YEAST

Accession

Primary (citable) accession number: P39516

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	December 15, 2009
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)