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P39515

- TIM17_YEAST

UniProt

P39515 - TIM17_YEAST

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Protein

Mitochondrial import inner membrane translocase subunit TIM17

Gene
TIM17, MIM17, MPI2, SMS1, YJL143W, J0648
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

GO - Molecular functioni

  1. P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: InterPro
  2. protein binding Source: IntAct

GO - Biological processi

  1. mitochondrial genome maintenance Source: SGD
  2. protein import into mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31587-MONOMER.
ReactomeiREACT_189012. Mitochondrial protein import.

Protein family/group databases

TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit TIM17
Alternative name(s):
Mitochondrial inner membrane protein MIM17
Mitochondrial protein import protein 2
Gene namesi
Name:TIM17
Synonyms:MIM17, MPI2, SMS1
Ordered Locus Names:YJL143W
ORF Names:J0648
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL143w.
SGDiS000003679. TIM17.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111Mitochondrial intermembrane Reviewed predictionAdd
BLAST
Transmembranei12 – 3221Helical; Reviewed predictionAdd
BLAST
Topological domaini33 – 5826Mitochondrial matrix Reviewed predictionAdd
BLAST
Transmembranei59 – 7921Helical; Reviewed predictionAdd
BLAST
Topological domaini80 – 878Mitochondrial intermembrane Reviewed prediction
Transmembranei88 – 10821Helical; Reviewed predictionAdd
BLAST
Topological domaini109 – 1124Mitochondrial matrix Reviewed prediction
Transmembranei113 – 13321Helical; Reviewed predictionAdd
BLAST
Topological domaini134 – 15825Mitochondrial intermembrane Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: Reactome
  3. mitochondrial inner membrane presequence translocase complex Source: SGD
  4. mitochondrial intermembrane space Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi4 – 41D → R: Induces abolition of mitochondrial import of transit peptide-containing proteins; when associated with K-8. 1 Publication
Mutagenesisi8 – 81D → K: Induces abolition of mitochondrial import of transit peptide-containing proteins; when associated with K-4. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 158158Mitochondrial import inner membrane translocase subunit TIM17PRO_0000210296Add
BLAST

Proteomic databases

MaxQBiP39515.
PaxDbiP39515.

Expressioni

Gene expression databases

GenevestigatoriP39515.

Interactioni

Subunit structurei

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. The complex interacts with the TIM44 component of the PAM complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PAM16P429494EBI-9127,EBI-26019
PAM18Q079143EBI-9127,EBI-31963
TIM23P3289713EBI-9127,EBI-9136
TIM44Q018522EBI-9127,EBI-9141

Protein-protein interaction databases

BioGridi33615. 26 interactions.
DIPiDIP-2108N.
IntActiP39515. 11 interactions.
MINTiMINT-510240.
STRINGi4932.YJL143W.

Structurei

3D structure databases

ProteinModelPortaliP39515.

Family & Domainsi

Sequence similaritiesi

Belongs to the Tim17/Tim22/Tim23 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5596.
GeneTreeiENSGT00390000017780.
HOGENOMiHOG000183190.
KOiK17795.
OMAiMRAPVLG.
OrthoDBiEOG7F7WN2.

Family and domain databases

InterProiIPR005678. Tim17.
IPR003397. Tim17/Tim22/Tim23/PMP24.
[Graphical view]
PfamiPF02466. Tim17. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00980. 3a0801so1tim17. 1 hit.

Sequencei

Sequence statusi: Complete.

P39515-1 [UniParc]FASTAAdd to Basket

« Hide

MSADHSRDPC PIVILNDFGG AFAMGAIGGV VWHGIKGFRN SPLGERGSGA    50
MSAIKARAPV LGGNFGVWGG LFSTFDCAVK AVRKREDPWN AIIAGFFTGG 100
ALAVRGGWRH TRNSSITCAC LLGVIEGVGL MFQRYAAWQA KPMAPPLPEA 150
PSSQPLQA 158
Length:158
Mass (Da):16,584
Last modified:February 1, 1995 - v1
Checksum:i1760B08F3B60D378
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti33 – 331H → T in AAB32164. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77796 Genomic DNA. Translation: CAA54823.1.
S74018 Genomic DNA. Translation: AAB32164.1.
X87371 Genomic DNA. Translation: CAA60812.1.
Z49418 Genomic DNA. Translation: CAA89438.1.
AY557852 Genomic DNA. Translation: AAS56178.1.
BK006943 Genomic DNA. Translation: DAA08658.1.
PIRiS46257.
RefSeqiNP_012392.1. NM_001181576.1.

Genome annotation databases

EnsemblFungiiYJL143W; YJL143W; YJL143W.
GeneIDi853298.
KEGGisce:YJL143W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77796 Genomic DNA. Translation: CAA54823.1 .
S74018 Genomic DNA. Translation: AAB32164.1 .
X87371 Genomic DNA. Translation: CAA60812.1 .
Z49418 Genomic DNA. Translation: CAA89438.1 .
AY557852 Genomic DNA. Translation: AAS56178.1 .
BK006943 Genomic DNA. Translation: DAA08658.1 .
PIRi S46257.
RefSeqi NP_012392.1. NM_001181576.1.

3D structure databases

ProteinModelPortali P39515.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33615. 26 interactions.
DIPi DIP-2108N.
IntActi P39515. 11 interactions.
MINTi MINT-510240.
STRINGi 4932.YJL143W.

Protein family/group databases

TCDBi 3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBi P39515.
PaxDbi P39515.

Protocols and materials databases

DNASUi 853298.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL143W ; YJL143W ; YJL143W .
GeneIDi 853298.
KEGGi sce:YJL143W.

Organism-specific databases

CYGDi YJL143w.
SGDi S000003679. TIM17.

Phylogenomic databases

eggNOGi COG5596.
GeneTreei ENSGT00390000017780.
HOGENOMi HOG000183190.
KOi K17795.
OMAi MRAPVLG.
OrthoDBi EOG7F7WN2.

Enzyme and pathway databases

BioCyci YEAST:G3O-31587-MONOMER.
Reactomei REACT_189012. Mitochondrial protein import.

Miscellaneous databases

NextBioi 973615.
PROi P39515.

Gene expression databases

Genevestigatori P39515.

Family and domain databases

InterProi IPR005678. Tim17.
IPR003397. Tim17/Tim22/Tim23/PMP24.
[Graphical view ]
Pfami PF02466. Tim17. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00980. 3a0801so1tim17. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of the essential yeast protein MIM17, an integral mitochondrial inner membrane protein involved in protein import."
    Maarse A.C., Blom J., Keil P., Pfanner N., Meijer M.
    FEBS Lett. 349:215-221(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 77162.
  2. "SMS1, a high-copy suppressor of the yeast mas6 mutant, encodes an essential inner membrane protein required for mitochondrial protein import."
    Ryan K.R., Menold M.M., Garrett S., Jensen R.E.
    Mol. Biol. Cell 5:529-538(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI."
    Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D.
    Yeast 12:787-797(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. "The polytopic mitochondrial inner membrane proteins MIM17 and MIM23 operate at the same preprotein import site."
    Kuebrich M., Keil P., Rassow J., Dekker P.J.T., Blom J., Meijer M., Pfanner N.
    FEBS Lett. 349:222-228(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, TOPOLOGY.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17."
    Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P.
    Cell 120:817-829(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TIM23 COMPLEX.
  10. "Conserved N-terminal negative charges in the Tim17 subunit of the TIM23 translocase play a critical role in the import of preproteins into mitochondria."
    Meier S., Neupert W., Herrmann J.M.
    J. Biol. Chem. 280:7777-7785(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASP-4 AND ASP-8.

Entry informationi

Entry nameiTIM17_YEAST
AccessioniPrimary (citable) accession number: P39515
Secondary accession number(s): D6VW42, Q02310
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 3, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1210 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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