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P39515

- TIM17_YEAST

UniProt

P39515 - TIM17_YEAST

Protein

Mitochondrial import inner membrane translocase subunit TIM17

Gene

TIM17

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

    GO - Molecular functioni

    1. P-P-bond-hydrolysis-driven protein transmembrane transporter activity Source: InterPro
    2. protein binding Source: IntAct

    GO - Biological processi

    1. mitochondrial genome maintenance Source: SGD
    2. protein import into mitochondrial matrix Source: SGD

    Keywords - Biological processi

    Protein transport, Translocation, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31587-MONOMER.
    ReactomeiREACT_189012. Mitochondrial protein import.

    Protein family/group databases

    TCDBi3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial import inner membrane translocase subunit TIM17
    Alternative name(s):
    Mitochondrial inner membrane protein MIM17
    Mitochondrial protein import protein 2
    Gene namesi
    Name:TIM17
    Synonyms:MIM17, MPI2, SMS1
    Ordered Locus Names:YJL143W
    ORF Names:J0648
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL143w.
    SGDiS000003679. TIM17.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: Reactome
    3. mitochondrial inner membrane presequence translocase complex Source: SGD
    4. mitochondrial intermembrane space Source: Reactome

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi4 – 41D → R: Induces abolition of mitochondrial import of transit peptide-containing proteins; when associated with K-8. 1 Publication
    Mutagenesisi8 – 81D → K: Induces abolition of mitochondrial import of transit peptide-containing proteins; when associated with K-4. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 158158Mitochondrial import inner membrane translocase subunit TIM17PRO_0000210296Add
    BLAST

    Proteomic databases

    MaxQBiP39515.
    PaxDbiP39515.

    Expressioni

    Gene expression databases

    GenevestigatoriP39515.

    Interactioni

    Subunit structurei

    Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. The complex interacts with the TIM44 component of the PAM complex.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PAM16P429494EBI-9127,EBI-26019
    PAM18Q079143EBI-9127,EBI-31963
    TIM23P3289713EBI-9127,EBI-9136
    TIM44Q018522EBI-9127,EBI-9141

    Protein-protein interaction databases

    BioGridi33615. 26 interactions.
    DIPiDIP-2108N.
    IntActiP39515. 11 interactions.
    MINTiMINT-510240.
    STRINGi4932.YJL143W.

    Structurei

    3D structure databases

    ProteinModelPortaliP39515.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111Mitochondrial intermembraneSequence AnalysisAdd
    BLAST
    Topological domaini33 – 5826Mitochondrial matrixSequence AnalysisAdd
    BLAST
    Topological domaini80 – 878Mitochondrial intermembraneSequence Analysis
    Topological domaini109 – 1124Mitochondrial matrixSequence Analysis
    Topological domaini134 – 15825Mitochondrial intermembraneSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei59 – 7921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei88 – 10821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei113 – 13321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the Tim17/Tim22/Tim23 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5596.
    GeneTreeiENSGT00390000017780.
    HOGENOMiHOG000183190.
    KOiK17795.
    OMAiMRAPVLG.
    OrthoDBiEOG7F7WN2.

    Family and domain databases

    InterProiIPR005678. Tim17.
    IPR003397. Tim17/Tim22/Tim23/PMP24.
    [Graphical view]
    PfamiPF02466. Tim17. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00980. 3a0801so1tim17. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P39515-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSADHSRDPC PIVILNDFGG AFAMGAIGGV VWHGIKGFRN SPLGERGSGA    50
    MSAIKARAPV LGGNFGVWGG LFSTFDCAVK AVRKREDPWN AIIAGFFTGG 100
    ALAVRGGWRH TRNSSITCAC LLGVIEGVGL MFQRYAAWQA KPMAPPLPEA 150
    PSSQPLQA 158
    Length:158
    Mass (Da):16,584
    Last modified:February 1, 1995 - v1
    Checksum:i1760B08F3B60D378
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti33 – 331H → T in AAB32164. (PubMed:7919535)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77796 Genomic DNA. Translation: CAA54823.1.
    S74018 Genomic DNA. Translation: AAB32164.1.
    X87371 Genomic DNA. Translation: CAA60812.1.
    Z49418 Genomic DNA. Translation: CAA89438.1.
    AY557852 Genomic DNA. Translation: AAS56178.1.
    BK006943 Genomic DNA. Translation: DAA08658.1.
    PIRiS46257.
    RefSeqiNP_012392.1. NM_001181576.1.

    Genome annotation databases

    EnsemblFungiiYJL143W; YJL143W; YJL143W.
    GeneIDi853298.
    KEGGisce:YJL143W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77796 Genomic DNA. Translation: CAA54823.1 .
    S74018 Genomic DNA. Translation: AAB32164.1 .
    X87371 Genomic DNA. Translation: CAA60812.1 .
    Z49418 Genomic DNA. Translation: CAA89438.1 .
    AY557852 Genomic DNA. Translation: AAS56178.1 .
    BK006943 Genomic DNA. Translation: DAA08658.1 .
    PIRi S46257.
    RefSeqi NP_012392.1. NM_001181576.1.

    3D structure databases

    ProteinModelPortali P39515.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33615. 26 interactions.
    DIPi DIP-2108N.
    IntActi P39515. 11 interactions.
    MINTi MINT-510240.
    STRINGi 4932.YJL143W.

    Protein family/group databases

    TCDBi 3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

    Proteomic databases

    MaxQBi P39515.
    PaxDbi P39515.

    Protocols and materials databases

    DNASUi 853298.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL143W ; YJL143W ; YJL143W .
    GeneIDi 853298.
    KEGGi sce:YJL143W.

    Organism-specific databases

    CYGDi YJL143w.
    SGDi S000003679. TIM17.

    Phylogenomic databases

    eggNOGi COG5596.
    GeneTreei ENSGT00390000017780.
    HOGENOMi HOG000183190.
    KOi K17795.
    OMAi MRAPVLG.
    OrthoDBi EOG7F7WN2.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31587-MONOMER.
    Reactomei REACT_189012. Mitochondrial protein import.

    Miscellaneous databases

    NextBioi 973615.
    PROi P39515.

    Gene expression databases

    Genevestigatori P39515.

    Family and domain databases

    InterProi IPR005678. Tim17.
    IPR003397. Tim17/Tim22/Tim23/PMP24.
    [Graphical view ]
    Pfami PF02466. Tim17. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00980. 3a0801so1tim17. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification of the essential yeast protein MIM17, an integral mitochondrial inner membrane protein involved in protein import."
      Maarse A.C., Blom J., Keil P., Pfanner N., Meijer M.
      FEBS Lett. 349:215-221(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 77162.
    2. "SMS1, a high-copy suppressor of the yeast mas6 mutant, encodes an essential inner membrane protein required for mitochondrial protein import."
      Ryan K.R., Menold M.M., Garrett S., Jensen R.E.
      Mol. Biol. Cell 5:529-538(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI."
      Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D.
      Yeast 12:787-797(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    7. "The polytopic mitochondrial inner membrane proteins MIM17 and MIM23 operate at the same preprotein import site."
      Kuebrich M., Keil P., Rassow J., Dekker P.J.T., Blom J., Meijer M., Pfanner N.
      FEBS Lett. 349:222-228(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION, TOPOLOGY.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17."
      Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P.
      Cell 120:817-829(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TIM23 COMPLEX.
    10. "Conserved N-terminal negative charges in the Tim17 subunit of the TIM23 translocase play a critical role in the import of preproteins into mitochondria."
      Meier S., Neupert W., Herrmann J.M.
      J. Biol. Chem. 280:7777-7785(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ASP-4 AND ASP-8.

    Entry informationi

    Entry nameiTIM17_YEAST
    AccessioniPrimary (citable) accession number: P39515
    Secondary accession number(s): D6VW42, Q02310
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1210 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3