Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P39515 (TIM17_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial import inner membrane translocase subunit TIM17
Alternative name(s):
Mitochondrial inner membrane protein MIM17
Mitochondrial protein import protein 2
Gene names
Name:TIM17
Synonyms:MIM17, MPI2, SMS1
Ordered Locus Names:YJL143W
ORF Names:J0648
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length158 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.

Subunit structure

Component of the TIM23 complex, at least composed of TIM23, TIM17, TIM50 and TIM21. The complex interacts with the TIM44 component of the PAM complex. Ref.9

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein.

Miscellaneous

Present with 1210 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the Tim17/Tim22/Tim23 family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 158158Mitochondrial import inner membrane translocase subunit TIM17
PRO_0000210296

Regions

Topological domain1 – 1111Mitochondrial intermembrane Potential
Transmembrane12 – 3221Helical; Potential
Topological domain33 – 5826Mitochondrial matrix Potential
Transmembrane59 – 7921Helical; Potential
Topological domain80 – 878Mitochondrial intermembrane Potential
Transmembrane88 – 10821Helical; Potential
Topological domain109 – 1124Mitochondrial matrix Potential
Transmembrane113 – 13321Helical; Potential
Topological domain134 – 15825Mitochondrial intermembrane Potential

Experimental info

Mutagenesis41D → R: Induces abolition of mitochondrial import of transit peptide-containing proteins; when associated with K-8. Ref.10
Mutagenesis81D → K: Induces abolition of mitochondrial import of transit peptide-containing proteins; when associated with K-4. Ref.10
Sequence conflict331H → T in AAB32164. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P39515 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 1760B08F3B60D378

FASTA15816,584
        10         20         30         40         50         60 
MSADHSRDPC PIVILNDFGG AFAMGAIGGV VWHGIKGFRN SPLGERGSGA MSAIKARAPV 

        70         80         90        100        110        120 
LGGNFGVWGG LFSTFDCAVK AVRKREDPWN AIIAGFFTGG ALAVRGGWRH TRNSSITCAC 

       130        140        150 
LLGVIEGVGL MFQRYAAWQA KPMAPPLPEA PSSQPLQA 

« Hide

References

« Hide 'large scale' references
[1]"Identification of the essential yeast protein MIM17, an integral mitochondrial inner membrane protein involved in protein import."
Maarse A.C., Blom J., Keil P., Pfanner N., Meijer M.
FEBS Lett. 349:215-221(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 77162.
[2]"SMS1, a high-copy suppressor of the yeast mas6 mutant, encodes an essential inner membrane protein required for mitochondrial protein import."
Ryan K.R., Menold M.M., Garrett S., Jensen R.E.
Mol. Biol. Cell 5:529-538(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI."
Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D.
Yeast 12:787-797(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[7]"The polytopic mitochondrial inner membrane proteins MIM17 and MIM23 operate at the same preprotein import site."
Kuebrich M., Keil P., Rassow J., Dekker P.J.T., Blom J., Meijer M., Pfanner N.
FEBS Lett. 349:222-228(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, TOPOLOGY.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17."
Chacinska A., Lind M., Frazier A.E., Dudek J., Meisinger C., Geissler A., Sickmann A., Meyer H.E., Truscott K.N., Guiard B., Pfanner N., Rehling P.
Cell 120:817-829(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TIM23 COMPLEX.
[10]"Conserved N-terminal negative charges in the Tim17 subunit of the TIM23 translocase play a critical role in the import of preproteins into mitochondria."
Meier S., Neupert W., Herrmann J.M.
J. Biol. Chem. 280:7777-7785(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ASP-4 AND ASP-8.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X77796 Genomic DNA. Translation: CAA54823.1.
S74018 Genomic DNA. Translation: AAB32164.1.
X87371 Genomic DNA. Translation: CAA60812.1.
Z49418 Genomic DNA. Translation: CAA89438.1.
AY557852 Genomic DNA. Translation: AAS56178.1.
BK006943 Genomic DNA. Translation: DAA08658.1.
PIRS46257.
RefSeqNP_012392.1. NM_001181576.1.

3D structure databases

ProteinModelPortalP39515.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33615. 26 interactions.
DIPDIP-2108N.
IntActP39515. 10 interactions.
MINTMINT-510240.
STRING4932.YJL143W.

Protein family/group databases

TCDB3.A.8.1.1. the mitochondrial protein translocase (mpt) family.

Proteomic databases

MaxQBP39515.
PaxDbP39515.

Protocols and materials databases

DNASU853298.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL143W; YJL143W; YJL143W.
GeneID853298.
KEGGsce:YJL143W.

Organism-specific databases

CYGDYJL143w.
SGDS000003679. TIM17.

Phylogenomic databases

eggNOGCOG5596.
GeneTreeENSGT00390000017780.
HOGENOMHOG000183190.
KOK17795.
OMAMRAPVLG.
OrthoDBEOG7F7WN2.

Enzyme and pathway databases

BioCycYEAST:G3O-31587-MONOMER.
ReactomeREACT_118590. Mitochondrial Protein Import (yeast).

Gene expression databases

GenevestigatorP39515.

Family and domain databases

InterProIPR005678. Tim17.
IPR003397. Tim17/Tim22/Tim23/PMP24.
[Graphical view]
PfamPF02466. Tim17. 1 hit.
[Graphical view]
TIGRFAMsTIGR00980. 3a0801so1tim17. 1 hit.
ProtoNetSearch...

Other

NextBio973615.
PROP39515.

Entry information

Entry nameTIM17_YEAST
AccessionPrimary (citable) accession number: P39515
Secondary accession number(s): D6VW42, Q02310
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families