P39485 (DHG4_BACME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose 1-dehydrogenase 4
EC=1.1.1.47

Alternative name(s):
GLCDH-IV

Gene names

Name:

gdhIV

Organism

Bacillus megaterium

Taxonomic identifier

1404 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Beta-D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H.
Subunit structure	Homotetramer.
Miscellaneous	Prefers NAD to NADP; 2M NaCl enhances its pH and thermostability.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Molecular_function	glucose 1-dehydrogenase [NAD(P)] activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 261

261

Glucose 1-dehydrogenase 4

PRO_0000054612

Regions

Nucleotide binding

11 – 35

25

NAD By similarity

Sites

Active site

158

1

Proton acceptor By similarity

Binding site

145

1

Substrate By similarity

Secondary structure

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261

Helix Strand Turn

Details...

Helix

3 – 5

3

Beta strand

9 – 12

4

Turn

13 – 16

4

Helix

18 – 29

12

Beta strand

33 – 40

8

Helix

42 – 55

14

Beta strand

58 – 63

6

Helix

69 – 83

15

Beta strand

88 – 91

4

Helix

101 – 103

3

Helix

106 – 116

11

Helix

118 – 133

16

Beta strand

139 – 143

5

Helix

146 – 148

3

Helix

156 – 176

21

Helix

177 – 179

3

Beta strand

182 – 188

7

Beta strand

190 – 193

4

Helix

194 – 196

3

Helix

197 – 201

5

Helix

203 – 211

9

Helix

221 – 232

12

Helix

234 – 236

3

Beta strand

243 – 247

5

Helix

250 – 252

3

Helix

254 – 256

3

Sequences

	Sequence		Length	Mass (Da)
	P39485 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 6FBEC9397BCF417C	FASTA	261	28,157
	10 20 30 40 50 60 MYTDLKDKVV VITGGSTGLG RAMAVRFGQE EAKVVINYYN NEEEALDAKK EVEEAGGQAI 70 80 90 100 110 120 IVQGDVTKEE DVVNLVQTAI KEFGTLDVMI NNAGVENPVP SHELSLDNWN KVIDTNLTGA 130 140 150 160 170 180 FLGSREAIKY FVENDIKGNV INMSSVHEMI PWPLFVHYAA SKGGMKLMTE TLALEYAPKG 190 200 210 220 230 240 IRVNNIGPGA MNTPINAEKF ADPVQRADVE SMIPMGYIGK PEEVAAVAAF LASSQASYVT 250 260 GITLFADGGM TKYPSFQAGR G

References

[1]	"Cloning, nucleotide sequences, and enzymatic properties of glucose dehydrogenase isozymes from Bacillus megaterium IAM1030." Nagao T., Mitamura T., Wang X.H., Negoro S., Yomo T., Urabe I., Okada H. J. Bacteriol. 174:5013-5020(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IAM 1030.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D10626 Genomic DNA. Translation: BAA01476.1.

PIR

I40225.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3AUS	X-ray	2.00	A/B	1-261	[»]
3AUT	X-ray	2.00	A/B	1-261	[»]
3AUU	X-ray	2.00	A/B	1-261	[»]
3AY6	X-ray	2.10	A/B/C/D	1-261	[»]
3AY7	X-ray	1.90	A/B	1-261	[»]

ProteinModelPortal

P39485.

SMR

P39485. Positions 1-261.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

SABIO-RK

P39485.

Family and domain databases

Gene3D

3.40.50.720. 1 hit.

InterPro

IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]

Pfam

PF00106. adh_short. 1 hit.
[Graphical view]

PRINTS

PR00081. GDHRDH.
PR00080. SDRFAMILY.

PROSITE

PS00061. ADH_SHORT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DHG4_BACME

Accession

Primary (citable) accession number: P39485

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program