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P39485

- DHG4_BACME

UniProt

P39485 - DHG4_BACME

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Protein

Glucose 1-dehydrogenase 4

Gene
gdhIV
Organism
Bacillus megaterium
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Beta-D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei145 – 1451Substrate By similarity
Active sitei158 – 1581Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 3525NAD By similarityAdd
BLAST

GO - Molecular functioni

  1. glucose 1-dehydrogenase [NAD(P)] activity Source: UniProtKB-EC
  2. identical protein binding Source: IntAct
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

SABIO-RKP39485.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose 1-dehydrogenase 4 (EC:1.1.1.47)
Alternative name(s):
GLCDH-IV
Gene namesi
Name:gdhIV
OrganismiBacillus megaterium
Taxonomic identifieri1404 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Glucose 1-dehydrogenase 4PRO_0000054612Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-7977646,EBI-7977646

Protein-protein interaction databases

MINTiMINT-8386740.

Structurei

Secondary structure

1
261
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 53
Beta strandi9 – 124
Turni13 – 164
Helixi18 – 2912
Beta strandi33 – 408
Helixi42 – 5514
Beta strandi58 – 636
Helixi69 – 8315
Beta strandi88 – 914
Helixi101 – 1033
Helixi106 – 11611
Helixi118 – 13316
Beta strandi139 – 1435
Helixi146 – 1483
Helixi156 – 17621
Helixi177 – 1793
Beta strandi182 – 1887
Beta strandi190 – 1934
Helixi194 – 1963
Helixi197 – 2015
Helixi203 – 2119
Helixi221 – 23212
Helixi234 – 2363
Beta strandi243 – 2475
Helixi250 – 2523
Helixi254 – 2563

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3AUSX-ray2.00A/B1-261[»]
3AUTX-ray2.00A/B1-261[»]
3AUUX-ray2.00A/B1-261[»]
3AY6X-ray2.10A/B/C/D1-261[»]
3AY7X-ray1.90A/B1-261[»]
ProteinModelPortaliP39485.
SMRiP39485. Positions 1-261.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39485-1 [UniParc]FASTAAdd to Basket

« Hide

MYTDLKDKVV VITGGSTGLG RAMAVRFGQE EAKVVINYYN NEEEALDAKK    50
EVEEAGGQAI IVQGDVTKEE DVVNLVQTAI KEFGTLDVMI NNAGVENPVP 100
SHELSLDNWN KVIDTNLTGA FLGSREAIKY FVENDIKGNV INMSSVHEMI 150
PWPLFVHYAA SKGGMKLMTE TLALEYAPKG IRVNNIGPGA MNTPINAEKF 200
ADPVQRADVE SMIPMGYIGK PEEVAAVAAF LASSQASYVT GITLFADGGM 250
TKYPSFQAGR G 261
Length:261
Mass (Da):28,157
Last modified:February 1, 1995 - v1
Checksum:i6FBEC9397BCF417C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10626 Genomic DNA. Translation: BAA01476.1.
PIRiI40225.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10626 Genomic DNA. Translation: BAA01476.1 .
PIRi I40225.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3AUS X-ray 2.00 A/B 1-261 [» ]
3AUT X-ray 2.00 A/B 1-261 [» ]
3AUU X-ray 2.00 A/B 1-261 [» ]
3AY6 X-ray 2.10 A/B/C/D 1-261 [» ]
3AY7 X-ray 1.90 A/B 1-261 [» ]
ProteinModelPortali P39485.
SMRi P39485. Positions 1-261.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-8386740.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P39485.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, nucleotide sequences, and enzymatic properties of glucose dehydrogenase isozymes from Bacillus megaterium IAM1030."
    Nagao T., Mitamura T., Wang X.H., Negoro S., Yomo T., Urabe I., Okada H.
    J. Bacteriol. 174:5013-5020(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: IAM 1030.

Entry informationi

Entry nameiDHG4_BACME
AccessioniPrimary (citable) accession number: P39485
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Prefers NAD to NADP; 2M NaCl enhances its pH and thermostability.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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