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Protein

Glucose 1-dehydrogenase 4

Gene

gdhIV

Organism
Bacillus megaterium
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei145SubstrateBy similarity1
Active sitei158Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 35NADBy similarityAdd BLAST25

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.1.1.47. 656.
SABIO-RKP39485.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose 1-dehydrogenase 4 (EC:1.1.1.47)
Alternative name(s):
GLCDH-IV
Gene namesi
Name:gdhIV
OrganismiBacillus megaterium
Taxonomic identifieri1404 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000546121 – 261Glucose 1-dehydrogenase 4Add BLAST261

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-7977646,EBI-7977646

Protein-protein interaction databases

MINTiMINT-8386740.
STRINGi545693.BMQ_1051.

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 5Combined sources3
Beta strandi9 – 12Combined sources4
Turni13 – 16Combined sources4
Helixi18 – 29Combined sources12
Beta strandi33 – 40Combined sources8
Helixi42 – 55Combined sources14
Beta strandi58 – 63Combined sources6
Helixi69 – 83Combined sources15
Beta strandi88 – 91Combined sources4
Helixi101 – 103Combined sources3
Helixi106 – 116Combined sources11
Helixi118 – 133Combined sources16
Beta strandi139 – 143Combined sources5
Helixi146 – 148Combined sources3
Helixi156 – 176Combined sources21
Helixi177 – 179Combined sources3
Beta strandi182 – 188Combined sources7
Beta strandi190 – 193Combined sources4
Helixi194 – 196Combined sources3
Helixi197 – 201Combined sources5
Helixi203 – 211Combined sources9
Helixi221 – 232Combined sources12
Helixi234 – 236Combined sources3
Beta strandi243 – 247Combined sources5
Helixi250 – 252Combined sources3
Helixi254 – 256Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AUSX-ray2.00A/B1-261[»]
3AUTX-ray2.00A/B1-261[»]
3AUUX-ray2.00A/B1-261[»]
3AY6X-ray2.10A/B/C/D1-261[»]
3AY7X-ray1.90A/B1-261[»]
ProteinModelPortaliP39485.
SMRiP39485.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107EXU. Bacteria.
COG1028. LUCA.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 3 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P39485-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYTDLKDKVV VITGGSTGLG RAMAVRFGQE EAKVVINYYN NEEEALDAKK
60 70 80 90 100
EVEEAGGQAI IVQGDVTKEE DVVNLVQTAI KEFGTLDVMI NNAGVENPVP
110 120 130 140 150
SHELSLDNWN KVIDTNLTGA FLGSREAIKY FVENDIKGNV INMSSVHEMI
160 170 180 190 200
PWPLFVHYAA SKGGMKLMTE TLALEYAPKG IRVNNIGPGA MNTPINAEKF
210 220 230 240 250
ADPVQRADVE SMIPMGYIGK PEEVAAVAAF LASSQASYVT GITLFADGGM
260
TKYPSFQAGR G
Length:261
Mass (Da):28,157
Last modified:February 1, 1995 - v1
Checksum:i6FBEC9397BCF417C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10626 Genomic DNA. Translation: BAA01476.1.
PIRiI40225.
RefSeqiWP_013055759.1. NZ_LUCO01000002.1.

Genome annotation databases

GeneIDi9116445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10626 Genomic DNA. Translation: BAA01476.1.
PIRiI40225.
RefSeqiWP_013055759.1. NZ_LUCO01000002.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AUSX-ray2.00A/B1-261[»]
3AUTX-ray2.00A/B1-261[»]
3AUUX-ray2.00A/B1-261[»]
3AY6X-ray2.10A/B/C/D1-261[»]
3AY7X-ray1.90A/B1-261[»]
ProteinModelPortaliP39485.
SMRiP39485.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-8386740.
STRINGi545693.BMQ_1051.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi9116445.

Phylogenomic databases

eggNOGiENOG4107EXU. Bacteria.
COG1028. LUCA.

Enzyme and pathway databases

BRENDAi1.1.1.47. 656.
SABIO-RKP39485.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 3 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHG4_BACME
AccessioniPrimary (citable) accession number: P39485
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Prefers NAD to NADP; 2M NaCl enhances its pH and thermostability.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.