P39484 (DHG3_BACME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucose 1-dehydrogenase 3
EC=1.1.1.47

Alternative name(s):
GLCDH-III

Gene names

Name:

gdhIII

Organism

Bacillus megaterium

Taxonomic identifier

1404 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Beta-D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H.
Subunit structure	Homotetramer.
Miscellaneous	Prefers NAD to NADP; very unstable compared to the other isoenzymes and 2M NaCl enhances its pH and thermostability.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Molecular function	glucose 1-dehydrogenase [NAD(P)] activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Glucose 1-dehydrogenase 3		PRO_0000054611
Regions
	Nucleotide binding	11 – 35	25	NAD By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	145	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P39484 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7F50C7C5F53F5DB1	FASTA	261	28,202
	10 20 30 40 50 60 MYTDLKDKVV VITGGSTGLG RAMAVRFGQE EAKVVINYYN NEEEALDAKK EVEEAGGQAI 70 80 90 100 110 120 IVQGDVTKEE DVVNLVQTAI KEFGTLDVMI NNAGVENPVP SHELSLDNWN KVIDTNLTGA 130 140 150 160 170 180 FLGSREAIKY FVENDIKGNV INMSSVHEMI PWPLFVHYAA SKGGMKQMTE TLALEYAPKG 190 200 210 220 230 240 IRVNNIGPGA MNTPINAEKF ADPVQRADVE SMIPMGYIGK PEEVAAVAAF LASSQASYVT 250 260 GITLFADGGM TKYPSFQTGR G

References

[1]	"Cloning, nucleotide sequences, and enzymatic properties of glucose dehydrogenase isozymes from Bacillus megaterium IAM1030." Nagao T., Mitamura T., Wang X.H., Negoro S., Yomo T., Urabe I., Okada H. J. Bacteriol. 174:5013-5020(1992) [PubMed: 1629157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IAM 1030.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10625 Genomic DNA. Translation: BAA01475.1.
PIR	I40224.
3D structure databases
ProteinModelPortal	P39484.
SMR	P39484. Positions 1-261.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHG3_BACME

Accession

Primary (citable) accession number: P39484

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	December 14, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program